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Dobnik, David,Baebler, Spela,Kogovsek, Polona,Pompe-Novak, Marusa,Stebih, Dejan,Panter, Gabriela,Janez, Nikolaja,Morisset, Dany,Zel, Jana,Gruden, Kristina 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.4
Glucanases are enzymes regulating the size exclusion limit and permeability of plasmodesmata and play a role in biotic stress. In plant genomes, they are encoded as relatively large gene families divided into four classes. Most studies of plant virus interactions have focused on glucanases from classes I and II. In our study, we have evaluated the role of the ${\beta}$-1,3-glucanase class III (Glu-III) gene in the potato-potato virus $Y^{NTN}$ ($PVY^{NTN}$) interaction and implemented the findings to plant biotechnology application. Potato cultivars D$\acute{e}$sir$\acute{e}$e and Sant$\acute{e}$, which are tolerant and extremely resistant to $PVY^{NTN}$, respectively, were stably transformed with Agrobacterium tumefaciens harbouring constructs for Glu-III overexpression. Localization of Glu-III protein in patches within the cell wall was determined by tagging the Glu-III protein with green fluorescent protein. Transgenic and nontransgenic plants were challenged with $PVY^{NTN}$ and its multiplication and spreading was followed. Differences in viral spread were observed between transgenic lines overexpressing Glu-III and non-transgenic lines, with stronger and faster viral spread in transgenic D$\acute{e}$sir$\acute{e}$e, and some multiplication in transgenic Sant$\acute{e}$. In addition, the ability of Glu-III to improve in planta protein production after agroinfiltration was tested. The results have shown that Glu-III overexpression enables faster spreading of vectors between cells and better protein production, which could be beneficial in improving in planta protein production system using viral vectors.