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- 저자 : Sora Yun(윤소라) (검색결과 2 건)
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수상태양광 설치 등 수면활동에 관한 법규제의 타당성에 관한 연구
윤소라(Yun, Sora) 충북대학교 법학연구소 2019 과학기술과 법 Vol.10 No.2
Recently, floating solar power has come into the spotlight as a renewable energy. However, because of uncertainty of its environmental impact, conflict between green and green (regulation on environmental protection restricts renewable energy supply) has been caused. Even though there is no direct regulation regarding floating solar power, Water Supply and Waterworks Installation Act restricts installation of a floating solar power plant within water source conservation area. Therefore, currently the floating solar power plants has been installed and operated in reservoirs for agriculture or reservoirs for drinking water designated as non-water source conservation area. This study reviews whether it is proper to distinguish the water source conservation area and non-water source conservation area in regard to installation of the floating solar power plant, even though standards for designation of the conservation area are not specificly clear. In addition, it suggests measures to overcome the conflict between green and green, which are conservation of water quality and aquatic ecosystem and supply of floating solar power. Considering studies on environmental impact of floating solar power is in its primary stage, areas where environmental impact is estimated low should be selected as a site for the floating solar power plant, relevant study should be proceeded to accumulate data, and based on the data, unnecessary restriction should be relaxed. Moreover, by clarifying the standards for designation of the conservation area under the Water Supply and Waterworks Installation Act, regulation for differential restriction should be developed based on the standards in order to flexibly restrict the floating solar power in accordance with location, scale, structure, etc. Above measures should be based on that environmental impact of floating solar power should be considered importantly so that renewable energy should be utilized in a “environmental-friendly” way.
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Yeon Hee Choi(최연희),Jun Seung Lee(이준승),Sora Yun(윤소라),Hyung Suk Baik(백형석) 한국생명과학회 2015 생명과학회지 Vol.25 No.2
Pseudomonas syringae pv. tabaci 11528은 담배를 숙주로 하여 wildfire disease를 일으키는 식물 병원성 세균이다. P. syringae pv. tabaci psyR deletion mutant를 이용하여 swarming motility, tabtoxin 생산능, siderophore 생산능, AHL 생산능 등의 phenotypic test를 수행하였다. psyR deletion mutant는 wild-type 균주보다 swarming motility가 증가하였고, tabtoxin 생산 또한 증가하였다. 하지만 siderophore와 AHL 생산능은 감소하였고 virulence 또한 지연되었다. 이러한 결과로 PsyR이 QS regulator로 작용한다는 사실과 더불어 병원성 유전자의 조절에도관여한다는 것을 확인하였다. PsyR이 각각의 병원성 유전자의 발현을 조절하는 regulator들에게 미치는 영향을 전사단계에서 확인하기 위해 fur, gacA, psyI, prhI, prhA, hrpR, hrpA 유전자들을 정량적 real-time PCR (qRT-PCR)방법으로 확인하였다. 또한 PsyR에 의한 병원성 유전자 조절이 DNA상에 직접적으로 결합하여 일어나는 것인지 아니면 다른 경로를 통해 간접적으로 일어나는 것인지를 확인할 필요가 있어 정제한 PsyR 단백질과 병원성 관련 유전자들의 upstream region 서열을 이용하여 electrophoretic mobility shift assay (EMSA)를 수행한 결과 본 연구에서 선정한 병원성 관련 유전자들이 PsyR에 의해 직접적으로 조절되지는 않는다는 사실을 밝혔다. Pseudomonas syringae pathovar tabaci is a plant pathogenic bacterium that causes wildfire disease in tobacco plants. In P. syringae pv. tabaci, PsyI, a LuxI-type protein, acts as an AHL synthase, while primary and secondary sequence analysis of PsyR has revealed that it is a homolog of the LuxR-type transcriptional regulator that responds to AHL molecules. In this study, using phenotypic and genetic analyses in P. syringae pv. tabaci, we show the effect of PsyR protein as a quorum-sensing (QS) transcriptional regulator. Regulatory effects of PsyR on swarming motility and production of siderophores, tabtoxin, and N-acyl homoserine lactones were examined via phenotypic assays, and confirmed by quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR). Further qRT-PCR showed that PsyR regulates expression of these virulence genes in response to environmental signals. However, an upstream region of the gene was not bound with purified MBP-PsyR protein; rather, PsyR was only able to shift the upstream region of psyI. These results suggested that PsyR may be indirectly controlled via intermediate-regulatory systems and that auto-regulation by PsyR does not occur.
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