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젖소 말초혈액 림프구로부터 소백혈병 바이러스 배양 및 전자현미경적 관찰
윤순식,박중원,변재원,강문일,유한상,한홍율 韓國電子顯微鏡學會 2005 Applied microscopy Vol.35 No.1
국내 젖소의 54.2%가 BLV에 감염되어 있지만 현재까지 국내에서는 소백혈병 바이러스 (BLV) 입자를 확인한 연구 보고가 없기 때문에 BLV 항체 양성 소의 말초혈액림프구를 배양, BLV를 발현시켜 전자현미경으로 바이러스 입자를 검출하였고 배양조건에 따른 바이러스 발현율 및 발현 시간을 비교하였다. 검사 결과 전형적인 C-형 바이러스를 확인할 수 있었으며 BLV 단크론 항체를 이용한 면역염색결과 BLV 항원 양성으로 확인되었다. BLV 는 대부분 세포 외부에 분포하고 있었으며 세포질 막에서 생성, 발아되어 나오는 것도 관찰되었다. 전체 바이러스의 크기는 90~100 nm였으며 nucleocapsid는 40~60nm였다. 소태아혈청 (FBS)과 T- 및 B-림프구 분열촉진물질(mitogen)을 각각 첨가하여 배양한 결과 두 군 모두에서 BLV 발현이 확인되었다. Lipopolysaccharide 첨가군은 배양 12시간, Conconavalin A 첨가군은 배양 24시간에 각각 림프구의 10%에서 바이러스가 관찰되었다. 또한 FBS만 첨가한 군과 FBS와 mitogen을 모두 첨가하지 않은 군에서도 관찰되었으나 바이러스의 수는 적었다. 본 연구에서 확립된 BLV 배양 기법을 활용하면 BLV에 감염된 소 중 바이러스를 발현하는 소, 즉 전파능이 있는 개체를 찾아내어 우선적으로 도태할 수 있기 때문에 BLV 감염으로 인한 피해를 막는데 효과적으로 이용될 수 있을 것으로 사료된다. Many studies have been performed on the bovine leukemia virus (BLV) since bovine leukosis had been reported in 1968 in Korea. However, there was no report on the ultrastructural examination of BLV. An attempt to detect C-type viral particles in the cultured peripheral blood lymphocytes of Holstein-Friesian dairy cattle, was made to determine whether in vitro viral expression might be used as a reliable method to identify the cow which is likely to transmit BLV. In transmission electron microscopic (TEM) examination, the virus particles were found predominantly outside of the lymphocytes even though a few particles were also observed within the membrane bound cytoplasmic vacuoles. All of them were C-type particles consisting of a central, electron-dense core separated by a clear area from a limiting envelope with a unit membrane structure. Virus particles were easily detected in the lymphocyte which was cultured with medium supplemented with either T-lymphocyte mitogen (conconavalin A) or B-lymphocyte mitogen (lipopolysaccharide). Identical viral particles, although fewer, were also consistently present in the lymphocytes cultured with medium which was containing foetal bovine serum (FBS) only and which was containing neither FBS or mitogen. By contrast, no virus particle was detected in extensive examination of lymphocytes before culture. In conclusion, the BLV cultivation and detection methods established in this study could be used as a tool to identify and eliminate the cattle which can transmit the BLV.
(Soon Wook Choi),(Eun Jung Lee),(Young Seek Lee),(Young Sook Yoo) 생화학분자생물학회 2000 BMB Reports Vol.33 No.6
The nerve growth factor (NGF) induces neuronal differentiation and neurite outgrowth of PC12 cells, whereas epidermal growth factors (EGF) stimulate growth and proliferation of the cells. In spite of this difference, NGF-or EGF-treated PC12 cells share various properties in cellularsignaling pathways. These include the activation of the phosphoinositide (PI)-3 kinase, 70 kDa S6 kinase, and in the mitogen-activated protein (MAP) kinase pathway, following the binding of these growth factors to intrinsic receptor tyrosine kinases (RTKs). Therefore, many studies have been atkempted to access the critical signaling events in determining the differentiation and proliferation of PC12 cells. In this study, we investigated the cytoso6c phospholipase A₂ (cPLA₂) in neurite behavior in order to identify the differences of signaling pathways between the NFF-induced differentiation and the EGF-induced proliferation of PC12 cells. We have showed here that the cPLA₂ was translocated from cytosol to membrane only in NGF-treated cells. We also demonstrated that this translocation is associated with NGF-induced activation of phospholipase C-γ(PLC-γ), which elevates intracellular Ca^(2+) concentration. These results reveal that the translocation of cPLA₂ may be a requisite event in the neuronal differentiation of PC12 cells. Various phospholipase inhibitors were used to confirm the importance of these enzymes in the differentiation of PC12 cells. Neomycin B, a PLC inhibitor, dramatically inhibited the neurite outgrowth, and two distinct PLA₂ inhibitors, 4-bromophenacyl bromide (BPB) and arachidonyltritluoro-methyl ketone (AACOCF₃) also suppressed the neurite outgrowth of the cells, as well. Taken together, these data indicated that cPLA₂ is involved in NGF-induced neuronal differentiation and neurite outgrowth of PC12 cells.
Choi, Soon-Wook,Yu, Eun-Ah,Lee, Young-Seek,Yoo, Young-Sook Korean Society for Biochemistry and Molecular Biol 2000 Journal of biochemistry and molecular biology Vol.33 No.6
The nerve growth factor (NGF) induces neuronal differentiation and neurite outgrowth of PC12 cells, whereas epidermal growth factors (EGF) stimulate growth and proliferation of the cells. In spite of this difference, NGF-or EGF-treated PC12 cells share various properties in cellular-signaling pathways. These include the activation of the phosphoinositide (PI)-3 kinase, 70 kDa S6 kinase, and in the mitogen-activated protein (MAP) kinase pathway, following the binding of these growth factors to intrinsic receptor tyrosine kinases (RTKs). Therefore, many studies have been attempted to access the critical signaling events in determining the differentiation and proliferation of PC12 cells. In this study, we investigated the cytosolic phospholipase $A_2$ ($cPLA_2$) in neurite behavior in order to identify the differences of signaling pathways between the NGF-induced differentiation and the EGF-induced proliferation of PC12 cells. We have showed here that the $cPLA_2$ was translocated from cytosol to membrane only in NGF-treated cells. We also demonstrated that this translocation is associated with NGF-induced activation of phospholipase $C-{\gamma}(PLC-{\gamma})$, which elevates intracellular $Ca^{2+}$ concentration. These results reveal that the translocation of $cPLA_2$ may be a requisite event in the neuronal differentiation of PC12 cells. Various phospholipase inhibitors were used to confirm the importance of these enzymes in the differentiation of PC12 cells. Neomycin B, a PLC inhibitor, dramatically inhibited the neurite outgrowth, and two distinct $PLA_2$ inhibitors, 4-bromophenacyl bromide (BPB) and arachidonyltrifluoro-methyl ketone ($AACOCF_3$) also suppressed the neurite outgrowth of the cells, as well Taken together, these data indicated that $cPLA_2$ is involved in NGF-induced neuronal differentiation and neurite outgrowth of PC12 cells.
Relationship analysis of honeybee diseases and unexpected colony loss
Yun Sang Cho,Mi-Sun Yoo,A-Tai Truong,Thi-Thu Nguyen,So-Youn Youn,Se-Ji Lee,Su-Kyoung Seo,Chae I Oh,Soon-Seek Yoon 한국응용곤충학회 2023 한국응용곤충학회 학술대회논문집 Vol.2023 No.10
최근 국내에서는 꿀벌 대량소실 현상이 2022년부터 전국적으로 발생하고 있다. 우리나라 뿐 만 아니라, 전세계 적으로 양봉산업에 큰 위협이 되고 있는 봉군붕괴현상은 2016년 미국에서 세계 최초로 보고되었다. 국내에서는 2022년 민관 합동조사 결과, 이상기온, 응애, 말벌 등이 주요 원인으로 지목되었다. 대량소실 현상을 보인 양봉농 가와 정상 농가의 병원체 검출 비교 결과, 유의성있게 검출이 증가되는 병원체는 발견되지 않았다. 그러나, Tyrophagus mite, Trypanosome, Lake Sinai virus, Apis mellifera filamentous virus 등의 신종 응애, 원충 및 바이러 스 감염이 추가로 확인되었다. 국내에서 새롭게 감염이 확인된 기생충과 병원체가 대량소실, 나아가 봉군붕괴현 상에 직간접적으로 영향을 주었을 것으로 사료되며, 지속적인 조사와 연구개발을 통해 기후등 환경변화에 따른 신종 질병 검색과 대책을 마련해야 할 것이다.
Isolation and identification of Moraxella cuniculi from a rabbit with keratoconjunctivitis
Yang, Dong-Kun,Kim, Ha-Hyun,Yoo, Jae-Young,Lim, Suk-Kyung,Yoon, Soon-Seek,Cho, In-Soo The Korean Society of Veterinary Science 2017 大韓獸醫學會誌 Vol.57 No.3
A Gram-negative, catalase- and oxidase-positive, coccus-shaped bacterium was isolated from a rabbit with keratoconjunctivitis. Colonies of the isolate were round, smooth, and exhibited hemolytic activity on 5% sheep blood agar. Scanning electron microscopy revealed 0.4 to $0.5{\mu}m$ diameter oval cocci. Partial 16S rRNA gene (1446 bp) sequence analysis demonstrated the isolate had significant homology with the Moraxella cuniculi CCUG2154 strain isolated from a rabbit in Germany in 1973. Our isolate was designated as APQAB1701. Antibiotic susceptibility tests demonstrated that APQAB1701 was sensitive to 24 antibiotics; 3 of the antibiotics (nalidixic acid, spectinomycin, and colistin) had minimal inhibitory concentrations ${\geq}32{\mu}g/mL$ against the isolate.
Molecular Surveillance for Tick-Borne Rickettsial and Protozoal Infectious Diseases of Dog in Korea
Keun-Ho Kim(Keun-Ho Kim),Mi-Sun Yoo(Mi-Sun Yoo),Hyun-Ji Seo(Hyun-Ji Seo),Kyu-Won Kwak(Kyu-Won Kwak),Hyunkyoung Lee(Hyunkyoung Lee),Jung-Won Park(Jung-Won Park),Seunghee Lee(Seunghee Lee),Soon-Seek Yoo 한국예방수의학회 2017 한국예방수의학회 학술대회자료집 Vol.2017 No.-
Distribution of Dog Ticks in the Republic of Korea, 2017
Hyun-Ji Seo(Hyun-Ji Seo),Mi-Sun Yoo(Mi-Sun Yoo),Keun-Ho Kim(Keun-Ho Kim),Kyu-Won Kwak(Kyu-Won Kwak),Hyunkyoung Lee(Hyunkyoung Lee),Jung-Won Park(Jung-Won Park),Seunghee Lee(Seunghee Lee),Soon-Seek Yoo 한국예방수의학회 2017 한국예방수의학회 학술대회자료집 Vol.2017 No.-
중합효소연쇄반응을 이용한 돼지 증식성 장염 진단기법 확립
임숙경,이희수,우승룡,윤순식,문운경,이유영,고홍범,Lym, Suk-kyung,Lee, Hee-soo,Woo, Sung-ryong,Yoon, Soon-seek,Moon, Oun-kyong,Lee, Yoo-young,Koh, Hong-bum 대한수의학회 1999 大韓獸醫學會誌 Vol.39 No.1
Porcine Proliferative Enteropathy(PPE) is an infectious enteric disease and a major cause of economic loss in swine industry due to weight loss, poor growth and sudden death in growing and finishing pigs at 6 to 20 weeks of age. PPE has been diagnosed by clinical signs, syndrom and lesions in the intestine in Korea. However, the diagnostic method had several problems in the detection of infected or carrier pigs. Therefore, in this study, we established the polymerase chain reaction(PCR) which was a fast, specific and sensitive method for identification of Lawsonia intracellularis (L intracellularis). We designed and synthesized primer on the 16S rDNA and p78 gene encoding L intracellularis. Specificity of the method was confirmed by comparison of the PCR results using other enteric bacteria and the study has shown that PCR method was sensitive to detect 1ng of genomic DNA as a template. Identity of the PCR products was confirmed by comparison of pattern of restriction endonuclease analysis with restriction enzyme Hae III and Pst I. Also, the PCR method was applicable to the naturally affected pigs with PPE. Based on the results from this study, the PCR method could be used as a fast and specific diagnostic tool for PPE.