축우 부루셀라병의 ELISA 진단법에 관한 연구

임윤규,이두식,박전홍,양기천,김승호,김공식,현관종,김우택,이영순 濟州大學校 農科大學 動物科學硏究所 1993 動物科學論叢 Vol.8 No.1

Enzyme-linked Immuno sorbent Assay (ELISA) for the serological diagnosis oi Bruceiia abortus was developed and compared with plate aggluhnation test Cell wall antigen was extracted from Brucella abortus 1119-3 by sonicabon and with a sodium deoxychlate solution Optimum protein concentra tion of coating antigen 0.4㎍/100㎕ protein on each microtiter plate well. Horse radish peroxidase(HRP) labled protein-G was used as a tracer of reacted antibodies ELISA confirmed the agreeable results of 40 rases out of 43 cases by plate aggulutination test ELISA diagnosed positive cases (10 out of 12) and negatiw cases (1 out of 12) with dubious sera by plate agglutination test From this results EL ISA could be used for the early diagnostic tools of Brucellosis in cattle.

치과용 콤포짓트 레진의 적정중합을 위한 최소 광조사 시간 평가

임범순,이용근,김철위,최기열,이중배 大韓齒科器材學會 2004 대한치과재료학회지 Vol.31 No.1

The aim of this study was to estimate the minimum irradiation time for dental composites using a dynamic mechanical analyzer (DMA) and FT-IR. Six commercially available dental composites with A3 shade were tested: Heliomolar RO (Vivadent, Liechtenstein), Charisma (Kulzer, Germany), Herculite XRV Enamel (Kerr, USA), Aelitefil (Bisco, USA), Z100 (3M, USA), and Z250 (3M, USA). Storage modulus was measured by using DMA (StressTech Rheometer, Rheologica Instrument, Sweden) with fast oscillation mode (1 Hz). After disk-type (4 ㎜ ?1 ㎜) samples were irradiated with 500 mW/cm2 for 1, 2, 3, 4, 5, 10, 15, 20, and 30 s, storage modulus was recorded continuously for 60 min. Degree of conversion was also measured using FT-IR spectroscopy (FTS-165, Biorad Win-IR, Perkin-Elmer, USA) at 60 min after irradiation with same curing condition as DMA test. Sample irradiated with 500 mW/cm2 for 120 s was used as a control. The average of results for five specimens was compared using Tukey multiple comparison test (p=0.05) and the minimum irradiation time of composites was determined. The minimum irradiation time to get adequate polymerization was different depending on the dental composites. Both Z100 and Z250 require short irradiation times (5 s) and Charisma requires long irradiation time (15 s).

치과용 콤포짓트 레진의 중합수축 응력

임범순,이용근,김철위 대한치과기재학회 2001 대한치과재료학회지 Vol.28 No.2

AMESim를 이용한 유압 굴삭기용 Main Control Valve의 해석에 관한 연구

임태형,최종환,양순용,이병룡,안경관 한국공작기계학회 2003 한국공작기계학회 춘계학술대회논문집 Vol.2003 No.-

The hydraulic excavator has been a popular research object for automation because of its multi-workings and economic efficiency. When it works crane tasks, most of disasters happen. The objective of this paper is to design each components and to construct boom, arm, bucket circuit. These models modeled with AMESim show us change of variables and behavior of excavator. Simulation model will be used for simulator of excavator.

미국정치의 특색

임용순 한국외국어대학교 북미연구소 2000 영미연구 Vol.6 No.-

티타늄 표면처리에 따른 조골세포의 반응

임영준,이용근,임범순,김철위 大韓齒科器材學會 2004 대한치과재료학회지 Vol.31 No.1

It is well known that surface modification of titanium for implant has been shown to improve the adhesion, migration and differentiation of adjacent cells and tissues. This study presented the results of an experimental study on the interaction between MC3T3-E1 (mouse calvarian osteoblast cell line) cells and cp-titanium treated by thermal oxidation at four different temperatures after TiO2 blasting and NaOH treatment, which could provide the benefits of surface roughening and favorable oxide layer formation. No heat treatment group was used as a control. Cell interactions were studied using the combination of phase contrast microscope and scanning electron microscope. Potential cytotoxicity was tested by MTT assay. The response of osteoblast cells was different according to the condition of surface modifications. Thermal oxidation at 600℃ showed the best early cell attachment and calcium nodule formation compared to other groups. Thermal oxidation at 800℃ showed the worst cell attachment and proliferation. In general, osteoblast cells were attached to accompanying the formation of cellular process, spreaded and differentiated on the specimen surfaces. Calcium nodules were formed on the surface after these cells were developed multiple cell layers. These early osetoblast/titanium interactions might play a critical role in the osteoinduction stage of osseointegration. There was no statistically significant difference in cytotoxicity among the heat treated surfaces from the MTT assay(p>0.05). The results of microstructure analysis of the characterized cp-titanium surface of this study will be published separately.

Detection of Antibokies to Sendai Virus Using protein G-based ELISA

임윤규,우희종,이영순 濟州大學校 農科大學 動物科學硏究所 1993 動物科學論叢 Vol.8 No.1

We have established an enzyme-linked immunosorbent assay(EL1SA) using enzyme labeled protein G(Protein G-ELISA) to detect Sendai virus-specific antibodies in laboratory animals. Best results were obtained at fl 6.0 of diluent and at 1 to 10 dilution of sera. Antigen concen-tration for solid phase matrix was 5μg /㎖ and the conjugate of horseradish peroxidase (HRP) and prqtein G was used at 1 to 100 dilution. We, also, investigated the sensitivity and speci-ficity of protein G and protein A. Protein C showed relatively high O. D. values than protein A in general. Furthermore, only Protein G-ELISA could detect the Sendai virus specific anti-bodies in rat. though both methods were effective in other species, mouse and guinea pig. No siginificant nonspecific reation was observed at our experimental conditions in both methods.

간세포암의 크기와 알파 피토프로틴의 연관성

임효순,정용연,김재규,윤웅,강형근,박진균 全南大醫大 2001 전남의대학술지 Vol.37 No.4

치과용 합금의 부식성에 관한 연구

임범순,이용근,윤숙진,김철위 大韓齒科器材學會 1995 대한치과재료학회지 Vol.22 No.1

Corrosion of dental alloys in the oral cavity can affect the mechanical, esthetic properties and efficiency of metallic restorations, and has great influence upon the local and systemic health of patients. The purpose of this study was to clarify the corrosion behavior of various dental alloys, and to measure the galvanic currents between dissimilar alloys. Fifty-eight kinds of dental casting alloys such as twenty-nine high gold alloys, ten low gold alloys, fourteen silver-palladium alloys, five base metal alloys, and twenty kinds of ceramic alloys such as seventeen noble metal alloys, three base metal alloys, were used as the experimental alloys. Modified Fusayama's artificial saliva titrated to pH 6.7 with phosphoric acid was used as an electrolyte. The specimens were immersed in the modified artificial saliva of 37℃. The rest potentials, anodic polarization curves, corrosion potentials, and galvanic currents were measured by using the potentiostat / galvanostat and zero shunt ammeter. From the experiment, the following results were obtained : 1) The gold based alloys showed the high rest potentials and the low current densities within experimental potential range. 2) The rest potentials and the current densities of the palladium-based alloys were influenced by the content of platinum, palladium, and silver. 3) In the nickel-chromium base alloys, the formations of passivation film were influenced by chromium content. The copper-aluminum alloy showed very high current density which was more than 150 mV . 4) The corrosion potentials of ceramic alloys were lower than those of dental casting alloys, but the trend of anodic polarizations were generally similar to the other alloys. 5) The amount of galvanic current and the polarity in the bimetallic galvanic cell could not be predicted considering only the alloy composition. The galvanic current decreased while the measuring time passed, but flew continuously.

시판 요구르트 Starter의 생리적 특성

임용숙,김순희,이신호 대구효성가톨릭대학교 식품과학연구소 1994 식품과학지 Vol.6 No.-

시판 호상 요구르트의 품질 검사와 분리된 유산균의 starter 특성을 조사한 결과 시판 요구르트의 유산균 수는 9개 회사 시료중 1개 회사가 1.66×10^6CFU/g을 나타내었고 그외 8개 회사제품은 10^8~10^9CFU/㎖ 범위를 나타내었다. 제품의 pH 범위는 4.00~4.33으로 비교적 산미가 강하였으며, 적정산도는 1.063~1.321%였다. 순수 분리한 요구르트 starter의 산 생성 속도는 배양 시간이 경과함에 따라 적정산도가 모두 증가하는 것으로 나타났다. 특히, 배양 12시간째 starter B가 산 생성이 가장 좋았으며, starter E의 산 생성 속도가 가장 늦었다. 9개 회사 starter는 공히 위액과 담즙산에 대한 내성이 관찰되었으며 특히 D, E, G 균주의 경우 위액과 담즙산에 거의 생존하여 가장 내성이 강한 경향을 나타내었다. Viable cells, pH and titrable acidity of nine kinds of marketing gel-type yoghurts and acid production and survival in digestive fluids and bile salts of the isolated starters were investigated. Viable cells of one sample was 1.66×10^6(CFU/g) and other eight samples showed about 10^8 to 10^9(CFU/g). All samples tested had a strong acidic taste with the pH of 4.0 to 4.3 and titrable acidity with 1.063-1.321% lactic acid/㎖. Acid production of isolated yoghurt starter increased during incubation. After 12 hr incubation, starter B showed the highest acid production ability but starter E was the lowest. All of starters were survived in artificial gastric juice and bile salts, especially starter B, E and I showed the strong resistance in artificial gastric juice and bile salts.