Effect of Ionizing Radiation on Rat Tissue: Proteomic and Biochemical Analysis

Park*, Eui‐,Chul,Yoon, Jong‐,Bok,Seong*, Jin‐,Sil,Choi, Kyoung‐,Soo,Kong, Eung‐,Sik,Kim, Yun‐,Jeong,Park, Young‐,Mee,Park, Eun‐,Mi Taylor Francis 2006 Preparative biochemistry & biotechnology Vol.36 No.1

<P>Reactive oxygen species (ROS), generated by ionizing radiation, has been implicated in its effect on living tissues. We confirmed the changes in the oxidative stress markers upon irradiation. We characterized the changes in the proteome profile in rat liver after administering irradiation, and the affected proteins were identified by MALDI-TOF-MS and ESI-MS/MS. The identified proteins represent diverse sets of proteins participating in the cellular metabolism. Our results demonstrated that proteomics analysis is a useful method for characterization of a global proteome change caused by ionizing radiation to unravel the molecular mechanisms involved in the cellular responses to ionizing radiation.</P>

관여도에 따른 TV 광고의 컬러 표현 방법이 인지효과 및 광고반응에 미치는 영향

박현수,이윤철 한국방송광고공사 2008 광고연구 Vol.0 No.80

The purpose of this study is to determine how color-expressing methods in TV advertisements affect cognitive effects and advertising responses according to involvement. The color-expressing methods were classified into color advertisements, partial color advertisements, and blackand-white advertisements; indexes for advertising responses were limited to good quality perception for a product, attitudes toward the advertisement, attitudes toward the brand, attitudes toward the sponsor, and purchasing intention. College students participated in this experiment were divided into three experimental groups, each of which consisted of 90 students. The three experimental groups were divided according to different kinds of color (blackand-white, color, partial color) and involvement (high- or low-involvement). The results demonstrated that partial-color advertisements were effective in cognitive effects, compared with color advertisements, as partialcolor ones showed statistically significant differences in all measurements. The high-involvement group perfectly supported the hypothesis that there would no difference in advertising responses by the kinds of color, while the low-involvement group supported the hypothesis that there were more positive advertising responses to black-and-white and partial-color advertisements than to color advertisements. There was a significant difference in purchasing intention between low-involvement partial-color advertisements and low-involvement color advertisements.

동물의 장에서 분리한 Enterococcus sp.의 특성 및 분말화

박종진,변정수,조윤경,홍승서,이현수 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.4

의약품 및 동물약품으로 사용되고 있는 분말유산균을 개발하고자 동물의 장에서 산성 pH 및 담즙산에 대한 내성이 강하고 대장균 생육억제력이 좋은 Enterococcus faecium L20을 분리 동정하고 이를 이용하여 고농도의 생균이 함유된 분말 유산균을 제조하였다. 분리된 균주는 pH 3.0에서 90% 이상의 내성과 0.3% 담즙산이 함유된 배지에서 100%의 내성을 나타냈으며 MRS 배지에서 대장균과 혼합배양시 24시간 이내에 대장균을 사멸시켰다. 분리된 균주를 산업용배지에서 배양한 후 동결건조시켜 분말을 만들었을 때 생균수는 5.0×0^11/g 이상이었다. 이것은 18℃에 보관하였을 경우 11개월 동안 80%의 생존율을 나타내었다. In order to develop a lactic acid bacterial powder which can be used as a probiotic for human and animal, a lactic acid bacteria which has high resistance against low pH and ox-gall, and shows a good growth inhibition against low pH and ox-gall, and shows a good growth inhibition against E. coli, was isolated from an animal intestine and characterized. The isolated strain was identified as Enterococcus faecium. It had more than 90% of survival at low pH for 2 hours and almost 100% of survival in the presence of 0.3% ox-gall. When co-cultured with E. coli in MRS broth, all of the E. coli cells were killed within 24 hours. The final powdered product of the isolated strain was manufactured after a freeze drying process suing an industrial media, and then checked its stability. Its storage stability was 80% for 11 months at 18℃.

열 변화에 의한 구리전선의 미세조직 및 상 변화에 관한 연구(Ⅲ)

박성우,김윤회,김상현,박남규,김수철,김영만 內務部 國立科學搜査硏究所 2002 國立 科學 搜査 硏究所 年報 Vol.34 No.-

藥鍼用蜂毒液이 黑色腫細胞에 미치는 抗癌效果에 對한 分子生物學的 硏究

朴贊烈,南相水,金昌煥,李栽東,姜成吉,李潤浩,安秉哲 경희대학교 동서의학연구소 2001 東西醫學硏究所 論文集 Vol.2000 No.-

To study anti-cancer effect and molecular biological mechanism of bee venom for aqua-acupuncture, the effects of bee venom on cell viability, apoptosis, and cell cycle were analyzed using MTT assay, tryphan blue assay, [3H]thymidnine release assay, flow cytometric analysis, activity of caspase-3 protease activity assay, and immunocytometric analysis of PCNA. To explore whether anti-cancer effects of bee venom are associated with the transcriptional control of gene expression, quantitative RT-PCR analysis of apoptosis- and cell cycle-related genes was performed. The obtained results are summarized as follows: 1.The MTT assay demonstrated that cell viability was decreased by bee venom in a dose-dependant manner. 2.Significant induction of apoptosis was identified using tryphan blue assay. [³H]thymidine release assay, and flow cytometric analysis of sub G₁fraction. 3.In analysis of caspase-3 protease activity, the activity had increased significantly, in a dose-dependant manner. 4.Quantitative RT-PCR analysis of the apoptosis-related genes showed that Bcl-2 and Bcl-X□ were down-regulated whereas Bax was up-regulated by bee venom treatment. 5.In flow cytometric analysis of cell cycle and immunocytometric analysis of PCNA expression, cell numbers of G₁phase was increased by a dose-dependant manner. 6.In quantitative RT-PCR analysis of the cell cycle-related genes, p21, p27, and p57 were increased, while Cyclin D1, CDK4, c-Myc, c-Fos, and Histone H3 were decreased. In contrast, there were no remarkable changes in expression levels of CDC2 and c-Jun.

행동억제(behavioral inhibition) 연구의 최근 동향

박성연,박수연 이화여자대학교 생활환경대학 인간생활환경연구소 2004 인간생활환경연구소 논집 Vol.- No.2

The purpose of this study was to review empirical research on behavioral inhibition in children. This study examined a stream of preceding researches by taking a view of relationships of behavioral inhibition in children and characteristics of children and parents. Characteristics of children included temperament, sex, change of age, order of birth and characteristics of parents included parenting behavior such as sensitive parenting, controlled parenting, disapproval parenting, and overprotective Parenting. Moreover, this study analyzed thee cross-cultural researches of behavioral inhibition.

산지하천의 강우특성에 의한 부유물질 유출에 관한 연구

윤인규,박수진,최한규 강원대학교 산업기술연구소 2007 産業技術硏究 Vol.27 No.A

In this research, we have selected the region of Jawoon river that is the area of high land vegetable growing in the upper Soyang Reservoir and we have observed actual floating materials that generate negative nutrition and turbidity of the Reservoir water and the changes of water quality by raining of each month for one year of 2005 in order to monitor the relationship of pollution sources by the outflow of rain water. In addition, we also have conducted statistical inspection methods such as correlation analysis and regression analysis on strength of raining force and rain continuance time among the elements affecting the outflow of floating materials.

구내방사선사진의 프랙탈 분석을 이용한 골다공증 예측

박금미,정연화,나경수 대한구강악안면방사선학회 2005 Imaging Science in Dentistry Vol.35 No.1

Purpose : The purpose of this study was to investigate whether the fractal dimension and radiographic image brightness of periapical radiograph were useful in predicting osteoporosis. Materials and Methods : Ninety-two postmenopausal women were classified as normal, osteopenia and osteoporosis group according to the bone mineral density of lumbar vertebrae and periapical radiographs of both mandibular molar areas were taken. The ROIs of 358 areas were selected at periapical and interdental areas and fractal dimension and radiographic image brightness were measured. Results : The fractal dimension in normal group was significantly higher than that in osteoporosis group at periapical ROI (P<0.05). The radiographic image brightness in normal group was higher than that in osteopenia and osteoporosis group. There was significant difference not only between normal and osteopenia group (P<0.05) but also within osteopenia and osteoporosis group (P<0.01) at periapical ROI. Significant difference was observed not only between normal and osteopenia group but also between normal and osteoporosis group at interdental ROI (P<0.01). Positive linear relationship was weakly shown at Pearson correlation analysis between fractal dimension and radiographic image brightness. BMD significantly correlated with fractal dimension at periapical ROI (P<0.01), and BMD and radiographic image brightness significantly correlated at both periapical and interdental ROIs (P<0.01). Conclusion : This study suggests that the fractal dimension and radiographic image brightness of periapical ROI may predict BMD.

소장 질환이 의심되는 환자에서 캡슐내시경과 CT 소견의 비교

이성수,홍성숙,김정훈,김연수,김진오,권석범,박성태,장윤우,최득린,권귀향 순천향의학연구소 2007 Journal of Soonchunhyang Medical Science Vol.13 No.1

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Bioceramics 종합공영균이 과채류 생육에 미치는 영향

박평수,채윤석,최황 진주산업대학교 농업기술연구소 1997 農業技術硏究所報 Vol.10 No.-

Bioceramics 종합공영균양액의 처리가 오이의 생육 및 수량, 방울토마토와 부강고추의 생육에 미치는 영향을 연구하여 얻은 결과는 다음과 같다. Bioceramics 양액 시여구에는 대조구에 비해 오이, 방울토마토 및 부강고추의 엽수, 초장, 경경, 근장, 전체중량, 엽장 및 엽폭이 양호한 편으로 건강한 식물체를 생산할 수 있었고, 오이의 수확량도 상당히 많은 증수의 결과를 얻었다. 향후 Bioceramics 양액의 시여가 토양에 미치는 영향과 양액의 조성 및 시여방법 등에 관한 보다 세밀한 연구가 필요할 것으로 사료된다. This study was carried out to find the effect of bioceramic contained complex microorganisms on the growth and yields of cucumber, small bell type tomato and pepper plants. The number of the leaf, length of the plant and the root, total weight, the length and width of the leaf of treated plants had shown their significant effects than those of nontreated plants. Bioceramic solution produeced healthy plants and the higher yields especially in cucumber plants. It is required futher study for soil effectiness, the composition and applying methods of Bioceramic fertilizer.