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RISS 인기검색어
- 검색키워드
- 저자 : SongJeon Lee (검색결과 3 건)
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Influence of Factors during Superovulation on Embryo Production in Korean Holstein Cattle
LEE, WonYou,SONG, KilYoung,LIM, KwangTaek,LEE, SongJeon,LEE, ByeongChun,JANG, Goo Japanese Society of Veterinary Science 2012 The Journal of veterinary medical science Vol.74 No.2
<P>The aim of this study was to investigate the influence of key parameters (donor parity, milk production, post-parturient day, season and milk recording data) associated with efficiency of embryo recovery (ER) in Holstein cattle. Elite Holstein cows and heifers were selected for ER, while Holstein heifers were used as recipients. The numbers of transferable embryos (TEs) produced were not significantly different when analyzed in terms of donor parity, milk production, postparturient day and season. However, the numbers of TEs were significantly increased when the milk protein (%; P)/fat (%; F) ratio was over 0.95 and/or the milk urea nitrogen (MUN) was between 12 and 18 d<I>l</I>/m<I>l</I>. The results from ET showed no differences in pregnancy rates among Holstein heifers receiving other types, developmental stage codes and quality grades of embryos. The mean interval from ER to artificial insemination was 60.6 days. Moreover, 19 offspring that had milk recording data showed a similar milk yield performance to that of the donor cows. In conclusion, this study showed that in Holstein cows, embryos were recovered and transferred and resulted in production of viable calves. Furthermore, P/F ratio and MUN could be candidate indicators for selection of high-efficiency donor cows.</P>
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Application of GFP locus for gene Editing in Transgenic Cattle using CRISPR/Cas9 System
Soo-Young Yum,SongJeon Lee,Hyun-Min Kim,Choong-IL Lee,WooJae Choi,Ji-Hyun Lee,Hee-Soo Kim,Sang-Eun Hahn,Seong-Jin Kim,Goo Jang 한국수정란이식학회 2016 한국수정란이식학회 학술대회 Vol.2016 No.10
CRISPR/Cas9-induced knock-out/-in can be occurred at specific locus in the genome by non-homologous end joining (NHEJ) or homology directed repair (HDR). Here, we demonstrate the targeted insertion into the specific loci of embryo fertilized by semen from transgenic cattle via CRISPR/Cas9 system. Recently, we published on the efficient generation of transgenic cattle using the DNA transposon system (Yum et al. Sci Rep. 2016 Jun 21;6:27185). In the study, eight transgenic cattle were born following transposon-mediated gene delivery system (Sleeping Beauty and Piggybac transposon system) via microinjection. In the analysis of their genome stability using next-generation sequencing, there was no significant difference in the number of genetic variants between transgenic and non-transgenic cattle. All the transgenic cattle have grown up to date (the oldest age: 33 months old, the youngest age: 15 months old) without any health issue. One of transgenic male cattle expressing GFP reached puberty and semen was collected. Over 200 frozen semen straws were produced and some were used for in vitro fertilization (IVF). On seven days after IVF, expression of GFP was observed at blastocyst stage and was seen in 80% of the embryos. Another application is to edit the GFP locus of the transgenic cattle because long-term and ubiquitous expression of transgene didn’t affect their health. In one cell stage embryos produced using GFP frozen-thawed semen, microinjection of sgRNA for GFP, Cas9, together with donor DNA that included RFP and homology arms to link the double-strand break of sgRNA target site into fertilized eggs resulted in expression of RFP. This indicated that the GFP locus of transgenic cattle shows potential candidates for stable insertion of the functional transgene. Knock-out/-in for editing GFP locus using CRISPR-Cas9 might be a valuable approach for the next generation of transgenic models by microinjection. In conclusion, we demonstrated P-112 that transgenic cattle via transposon system are healthy to date and germ-line competence was confirmed. The GFP locus will be used as the potential target site for future gene engineering via genome-editing technology. Finally, all those animals could be a valuable agricultural and veterinary science resource for studying the effects of gene manipulation on biomedical research and medicine. This work was supported by BK21 PLUS Program for Creative Veterinary Science and Seoul Milk Coop (SNU 550-20160004).
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