위의 양성 및 악성병변에서의 Argyrophilic Nucleolar Organizer Regions발현에 대한 연구

송규상 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.2

The colloid silver staining technique to reveal argyrophilic nucleolar organizer regions (AgNOR) was performed in chronic atrophic gastritis, chronic gastritis with intestinal metaplasia, dysplasia, early gastric carcinoma, and advanced gastric carcinoma with or without regional lymph node metastasis. The results were as follows. 1. There was an increasing tendency of the mean number of AgNOR from benign lesions to malignant lesions. 2. The malignant lesions had significantly higher number of AgNOR than benign lesions. 3. The mean number of AgNOR in dysplasia was significantly higher than that of chronic atrophic gastritis. However, there was no statistical difference from other lesions. 4. There was no statistical difference between early gastric carcinoma and advanced gastric carcinoma or between advanced gastric carcinoma without metastasis and advanced gastric carcinoma with metastasis These results suggest that the number of AgNOR may have significance in differentiating between benign lesions and malignant lesions of the stomach, but may not be helpful for evaluating the depth of invasion and metastasis.

타액선 종양의 면역조직화학적 연구

송규상 충남대학교 의과대학 지역사회의학연구소 1989 충남의대잡지 Vol.16 No.2

An immunohistochemical study was performed on the normal salivary gland, pleomorphic adenomas, mucoepidermoid carcinomas, adenoid cystic carcinomas, acinic cell tumor, and Warthin’s tumor. PAP kits for S-100 protein, vimentin, and epithelial membrane antigen were used and the results were as follows. 1. Myoepithelial cells of the normal salivary gland were positive for S-100 protein and vimentin, negative for EMA. Duct cells were stained positively for S-100 protein and EMA. Acinic cells were negative for all stains. 2. Neoplastic myoepithelial cells were strong positive for S-100 protein and vimentin. Areas of ductal differentiation were positive for EMA and occasionally positive for S-100 protein and vimentin. 3. Mucin secreting cells and squamous cells of mucoepidermoid carcinoma showed posive reaction for EMA. Intermediate cells revealed weak positivity for S-10 protein and EMA and were positive for vimentin. 4. Adenoid cystic carcinoma showed occasional positivity for S-100 protein and vimentin. Ductal areas were positive for EMA. 5. Warthin’s tumor showed positive reaction in epithelial cells for EMA. Acinic cell tumor were negative for all antibodies. These results suggested that the coexpression of S-100 protein and vimentin is useful in identify-ing the neoplastic myoepithelial cells and the myoepithelial cells play an important role in the histogenesis of pleomorphic adenomas, mucoepidermoid carcinomas, and adenoid cystic carcinomas.

유방병변에서의 Nucleolar Organizer Regions에 대한 연구

송규상 충남대학교 의과대학 지역사회의학연구소 1992 충남의대잡지 Vol.19 No.2

To study the proliferative activity of cells in benign and malignant breast lesions, a one step silver staining technique to identify argyrophilic nucleolar organizer regions had been applied in 11 cases of fibrocystic change, 11 cases of fibroadenoma, 15 cases of invasive ductal carcinoma without metastasis, 9 cases of invasive ductal carcinoma with metastasis, and 3 cases of medullary carcinoma, respectively. The results were as follows: 1. AgNORs in both fibrocystic disease and fibroadenoma were relatively small and uniform in size and shape. Epothelial cells in fibrocystic change had 1.49 AgNORs per cell in average Stromal cells and epithelial cells in fibroadenoma contained 1.58 AgNORs and 2.02 AgNORs per cell in average, respectively. 2. Invasive ductal carcinomas and medullary carcinomas showed relatively irregular AgNORs. The mean numbers of AgNORs in invasive carcinoma with or without metastasis were 3.23 and 3.41 per cell. Medullary carcinomas contained 3.18 AgNORs per cell. There were no statitical differences among malignant tumors. 3. The numbers of AgNORs were statistically different between benign and malignant lesions. These data suggested that the mean AgNORs counts was useful in differentiation between benign and malignant breast lesions. However, it seemed that they did not correlate with malignant types and status of metastasis.

뇌종양의 혈관 생성과 세포 소멸에 대한 연구

송규상 충남대학교 의과대학 지역사회의학연구소 1996 충남의대잡지 Vol.23 No.2

To study angiogenesis and apoptotic indices in the brain tumors, 33 cases of brain tumor(13 cases of astrocytoma, 10 cases of glioblastoma multiforme and 10 cases of meningioma) were seldcted and stained with anti-factor Ⅷ monoclonal antibody and in situ apoptosis detection kit(Apoptag). Among brain tumors glioblastoma multiforme showed the highest angiogenic activity(25.4%). The average percentages of endothelial cells in grade I, Ⅱ, Ⅲ astrocytomas were 3.2, 5.6, 8.4%, respectively and 18.4% in meningioma. Apoptotic indices were highest in glioblastoma multiforme(2.7), followed by grade Ⅲ astrocytoma and meningioma. The results suggest that the glial tumors(astrocytoma and glioblastoma multiforme) seem to show increasing tendency of angiogenesis and apoptotic index ac cording to the degree of malignancy.

백서의 복강내에 투여한 Mitomycin-C-Polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

송규상,강대영,최정목,노승무,정경수,오정연,김진향,양준목,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

To evaluate the in-vivo effects of mitomycin-C-polyglycolic acid composite(MMC-PGA) the disk: shaped composite were implanted into the peritoneal cavity of the Sprague-Dawley rats. The pathologic changes were examined at various time points up to 12 weeks. Initially the soft tissue around the inserted disks showed capillary congestion at 3 days. Inflammatory infiltrates with foreign body giant cells appeared from the 2nd week and reached peak response at 6-8 weeks. These reaction diminished prominently at 12 weeks. No specific pathologic change was found in the liver, the kidneys, and the heart. The above results suggest that the MMC-PGA composite can serve as a new device for intraperitoneal chemotherapy of various types of cancers.

질소오염 감소를 위한 질소수지표 개발에 관한 연구 : 충청북도 사례연구 For Province Chungcheongbuk-do

송재옥,홍성규 建國大學校 自然科學硏究所 2004 建國自然科學硏究誌 Vol.15 No.-

These days, the environment has been polluted as a result of many kinds of agricultural activities, such as over-fertilization and excessively injected agricultural medicine and livestock-wastewater. Especially, superfluously used nitrogenous fertilizers have worsened both ground water and earths surface water. In Korea, compared to other developed countries, there has been an extensive use of nitrogenous fertilizer, affecting the cleanliness of ground water. Under these circumstances, it is imperative to study the pollution by nitrogenous fertilizer. In order to estimate the extent of potential pollution by nitrogenous fertilizer, this study establishes a nitrogen parameter by providing the balance between the inputted quantity of nitrogen element (marked as plus) and the output quantity of nitrogen element (marked as minus). For the calculation of surface nitrogen balance, nitrogen input was calculated by fertilizer consumption and cattle manure input etc., and nitrogen output was mainly harvested crop products. This balance, by indicating the extent of potential pollution, may be used to improve the effectiveness of environment policy making. In Province Chungcheongbuk-do(Chungbuk), a surplus of nitrogen is 15.3kg/10a. However, each region has different levels of surplus based on the amounts of nitrogenous fertilizer used, soil and climate. We observe that the policy to reduce pollution by nitrogen must be applied based on environmental characteristics of each region. In conclusion, the nitrogen parameter has significance in planning environmental policy that is appropriate to each region, by helping to analyze the effect on rural household income and to solve other environment-related problems.

위 MALT 림프종에서 다발성 림프절 전이로 오인된 동시성 림프절 결핵 1예

김성은,김규종,김도현,송준영,문대성,장리라,유찬희,문원,박무인,박선자,김영옥 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.2

28세 남자가 내시경적 점막절제술을 통해 저등급 위MALT 림프종으로 진단되었다. 병기설정을 위해 18F-FDG PET 검사를 시행하였고 우측 쇄골상부, 우측상/하부 기관주위, AP window 부위에 강한 섭취가 관찰되어 전이성 림프절로 판단하였다. 그러나, 우측 쇄골상부 림프절에서 시행한 절제생검에서 만성육아종성염증소견이 관찰되었고 조직의 결핵 중합효소연쇄반응에서도 양성 반응을 보였다. 2개월간 항결핵제의 사용 후 시행한 추적 컴퓨터단층촬영 검사에서 림프절 크기의 감소를 보여 최종적으로 위 MALT 림프종에서 광범위 림프절 전이로 오인된 동시성 림프절 결핵으로 진단되었다. A 28-year-old man was diagnosed as gastric MALT lymphoma in diagnostic EMR (endoscopic mucosal resection). He subsequently underwent an 18F-FDG PET. 18F-FDG PET showed intense multifocal uptake in right supraclavicular, right upper and lower paratracheal, and AP window areas that was considered radiologically to represent widespread metastatic lymphadenopathy. Excisional biopsy of a supraclavicular lymph node, however, revealed chronic granulomatous lymphadenitis with a positive reaction of Tb-nested PCR. After antituberculosis treatment for 2 months, a follow-up CT scan showed complete resolution of the lesions. Intense 18F-FDG uptake could be due to an infectious process such as synchronous tuberculous lymphadenitis. Therefore, this could mimic a malignant condition such as lymphoma with extensive lymph node metastasis due to a false-positive finding, which may lead to a misdiagnosis.

지속성 외래 복막투석 환자에서 염증 지표와 고해상도 B 모드 경동맥 초음파 소견의 연관성에 관한 연구

송현용,송영수,안철우,강신욱,최규헌,하성규,이현철,이호영,한대석 대한신장학회 2002 Kidney Research and Clinical Practice Vol.21 No.2

배 경 : 저알부민혈증 및 C-반응 단백(C-reactive protein, CRP)이 증가된 지속성 외래 복막투석 환자의 사망률이 높은 정확한 이유는 밝혀져 않다. 혈액투석 환자에서처럼 복막투석 환자에서 저알부민혈증 및 염증반응이 심혈관계 질환 위험인자 인지 알아보고자 하였다. 방 법: 비당뇨병성 말기 신부전, 지속성 외래 복막투석 환자 93명의 심혈관계 질환 위험인자들과 복막 투과 특성 및 알부민, CRP 등의 염증지표에 따라 고해상도 초음파를 이용한 경동맥내막-중간막 두께, 내막-중간막 면적, 플라크의 유무 등을 단면적으로 비교하였다. 결 과: 복막 투과 특성은 고투과막군 14명, 중간 고투과막군 38명, 중간 저투과막군 31명, 저투과막군 10명으로 각 군간의 경동맥 내막-중간막 두께, 플라크 유무 및 내막-중간막 면적의 차이는 없었다. 기존 관상동맥 질환 환자는 8예(8.6%)로 내막-중간막 두께는 관상동맥 질환이 없는 환자보다 증가되었고(0.79±0.21 vs 0.60±0.11 mm, p<0.05) 플라크 형성은 더 많이 보이나 통계적 유의성은 없었다(75.0 vs 63.5%, p>0.05). 혈청 알부민치와 경동맥 내막-중간막 면적은 역상관관계를 보였다(r=-0.27, p<0.05). 저알부민혈증 환자(혈청 알부민 <3.5 g/dL)는 정상 알부민혈증 환자보다 내막-중간막 두께가 증가되었고(0.67±0.15 vs 0.61±0.12 mm, p<0.05), 플라크도 유의하게 많았다(68.0 vs 55.8%, p<0.05). 혈청 CRP치와 경동맥 내막-중간막 면적은 양의 상관관계가 있었으며(r=0.21, p<0.05) CRP가 높은 환자(≥0.8 mg/dL, n=18)에서플라크가 많이 관찰되었으나(65.8 vs 50.0%, p<0.05) 경동맥 내막-중간막 두께 및 내막-중간막면적은 양군간에 차이가 없었다. 다변량 회귀 분석상 경동맥 내막-중간막 두께에 영향을 미치는 인자는 고령, 높은 CRP, 기존 심혈관계질환, 낮은 혈청 알부민 순이였다. 결 론 : 복막투석 환자에서 고해상도 초음파를 이용한 경동맥 내막-중간막 두께 측정은 심혈관계 질환 유무 예측의 비침습적 검사가 될 수 있고 저알부민혈증 및 높은 CRP과 같은 만성염증 지속상태가 복막투석 환자에서 죽상 동맥 경화증의 발생을 증가시킨다고 가정할 수 있다. Background : Continuous ambulatory peritoneal dialysis(CAPD) patients with low albumin(LA) and signs of inflammation reflected by increased C-reactive protein(CRP) level have an increased mortality, but the mechanism of this phenomenon is not clear yet. Methods : To answer whether LA and inflammation also enhance cardiovascular risk in CAPD patients, we performed cross sectional study measuring carotid artery intima-media thickness(IMT), calculated intima-media area(cIM area) and the presence of plaque by high-resolution B-mode ultrasonography in 93 non-diabetic CAPD patients. Results : Patients with coronary artery disease (CAD, n=8) had significantly increased IMT(0.79± 0.21 mm vs. 0.60±0.11 mm, p<0.05) and higher prevalence of carotid plaques(75.0% vs. 63.5%) compared to the non-CAD patients. Significant inverse correlation was observed between serum albumin (SA) level and cIM area(r=-0.27, p<0.05). Those patients with LA(SA <3.5 g/dL) had significantly increased IMT compared to non-LA patients(0.67±0.15 mm vs 0.61±0.12 mm, p<0.05). Prevalence of carotid plaques was also significantly higher in LA patients (68.0% vs. 55.8%, p<0.05). CRP level revealed a significant positive correlation with cIM area(r=0.21, p< 0.05). Patients with high CRP(≥0.8 mg/dL, n=18) had higher prevalence of carotid plaques (65.8% vs. 50.0%, p<0.05) compared to those patients with CRP <0.8 mg/dL, but IMT and cIMT area were not different. By multivariate logistic regression analysis, old age, high CRP, history of CAD and low SA were the independent risk factors affecting IMT. Conclusion : Our study strongly suggests that low albumin and chronic inflammatory state of CAPD patients could be associated with increasing atherosclerotic cardiovascular disease. (Korean J Nephrol 2002;21(2):285-294)

여성 알코올 의존 환자의 임상적 특성에 관한 연구 : - 일 정신병원 입원환자를 중심으로 -

성상경,신동환,송종호,이규항 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.6

연구목적: 남. 여 알코올의존 환자의 인구통계학적 특성, 음주력, 가족력, 음주양상, 음주동기, 공유질병 등을 포함하는 임상적 특성의 차이 및 여성 알코올의존에 대한 질병양상과 그 가족의 태도를 파악함으로써 향후 여성 알코올의존 환자의 치료 대책수립을 위한 기초자료를 제공하는데 그 목적이 있다. 방 법: 1991년 1월1일부터 1996년 5월31일까지 일 정신병원에 입원 치료한 남. 여 각각 51명의 알코올의존 환자를 연구대상으로 하였으며, 연구방법으로 병력기록지 및 설문지 조사를 통한 자료 수집을 이용하였다. 양군간의 비교를 위해 남. 여 인원 및 나이를 통제하였고, 여성 알코올의존자의 평균 연령은 44.45±11.02세였고 남성 환자군의 평균 연령은 44.17±10.08이었다. 진단은 DSM-Ⅲ-R에 의해 이루어졌으며 대상자 모두는 알코올 의존의 진단기준을 만족하였다. 결 과: 여성 알코올의존자는 남성 알코올의존자에 비해 음주시작 연령이 늦었고 과거 음주기간은 짧았으며 일 음주량이 적었다. 그리고 여성 알코올의존자는 남성 알코올의존자에 비해 자발적 입원이 많았으며 입원후 금단증상, 알코올성 간염, 당뇨등 신체적 합병증이 적었다. 또한, 입원후 가족의 태도에서 가족의 이혼위협 등 치료에 비협조적인 경우가 남성 알코올의존자에 비해 많았으며, 과거력상 사별, 이혼, 남편의 구타 등 상실의 경험이 많았다. 결 론: 남. 여 알코올의존자간에 인구통계학적 특성, 임상 특성, 가족의 태도 등 여러가지 면에서 차이가 나는 것으로 나타났다. 여성 알코올의존자 집단은 가족의 죽음, 부부생활의 갈등과 같은 심리 사회적 좌절 경험이 더 많고 가족들이 더 비협조적이었다. 그러므로 치료자는 이러한 여성알코올의존자의 특성을 치료와 예방에 반영하고 이들에 대한 세심한 배려를 해야할 것이다. Objectives: This study aimed to provide a basic data of female alcoholics by understanding the gender differences of the clinical characteristics including demographic characteristics, alcoholic history, family history, drinking patterns, reasons for drinking, and comorbidity in male and female alcoholics. It also ained to establish the therapeutic plans through understanding the patterns of disease and the patterns of family cooperation in female alcoholics. Methods: The study subjects consisted of 51 male and female alcoholics admitted to a mental hospital from January 1, 1991 to May 31, 1996. Subjects were age-and sex-matched. We reviewed patients charts and administered questionaires. Mean age of female alcoholics was 44.45±11.02 years and that of male alcoholics was 44.17±10.08 years. All male and female patients met with the DSM-Ⅲ-R criteria of alcohol dependence. Results: Female alcoholics were later in drinking-onset age, and to had shorter duration of previous drinking history and less average amount of daily drinking than male alcoholics. And female alcoholics were found to seek treatment more voluntarily, and to have less frequent delirium tremens after admission and less medical complications such as hepatitis and diabetes mellitus. Also female alcoholics had more frustration experiences of separation such as death of family member, divorce, and husband`s physical abuse. Female alcoholic`s families had more uncooperative attitude toward treatment such as threat to divorce after admission. Conclusions: There were many differences between male and female alcoholics of demographic characteristics, clinical characteristics, and patterns of family cooperation. Female alcoholics were found to have more experiences of psychosocial frustration including death of family member and marital conflicts, and more uncooperative attitude of her family members. Therefore, it is required that therapists consider the characteristics of female alcoholics upon treatment and prevention, and have more concern about female alcoholics.

황성 섬강 상류의 어류상

송호복,권오길,전상호,김휘중,조규송 江原大學校 附設 環境硏究所 1996 環境硏究 Vol.13 No.-

Fish fauna was investigated from June to November 1993 at the upper Sum river in Hoengsong. Form of stream was Aa and Aa-Bb, and pH, DO and BOD were 7.3~7.9, 8. 8~10.2mg/1 and 1.3~l. 5mg/1. The collected fishes were classified twenty nine species belonging to twenty two genera and seven family , of witch twelve species were endemic species of Korea. Dominant species was Zacco temmincki and subdominant species was Coreoleuciscus splendus. Zacco platypus, Microphgsogobio longidorsalis and Acheilognathus signifer were also numerous. Scarce species were Hemibarbus labeo, Coditis lutheri and the like. The species diversity index, dominance index and evenness index were 2.552, 0.384 and 0.758.