A New Sesame Variety, “Kangbaek” with Disease Resistance, Lodging Tolerence and High Yield

Kang-Bo Shim,Churl-Whan Kang,Suk-Bok Pae,Si-Kyu Lim,Yu-Young Lee,Duck-Yong Suh,Jae-Whan Rho,Jin Song,Dong-Whi Kim,Ho-Young Kim 한국육종학회 2005 한국육종학회지 Vol.37 No.5

A new sesame variety Kangbaek was developed from Yeongnam Agricultural Research Institute in 2004. Cros wasmade by lodging resistant SP9003-3 line to the F1with high yielding capacity, folowed by pedigree selection, yield test and RYT by the sesame breeding team at the National Insti-tute of Crop Science up to 2003. The variety showed higher disease resistance, especially phytophothra disease, and lodging resis-respectively. It's 1000 grains weight was about 2.54 g indicating 0.03 g heavier than that of Yangbaekkae, and it's oil content wasabout 52%. Kangbaek also contained total 7.65mg/g of such lignans as sesamin and sesamolin. The average yield of Kang-baek was 82 kg per 10a at the national-wide regional performance.

지속성 외래 복막투석 환자에서 염증 지표와 고해상도 B 모드 경동맥 초음파 소견의 연관성에 관한 연구

송현용,송영수,안철우,강신욱,최규헌,하성규,이현철,이호영,한대석 대한신장학회 2002 Kidney Research and Clinical Practice Vol.21 No.2

배 경 : 저알부민혈증 및 C-반응 단백(C-reactive protein, CRP)이 증가된 지속성 외래 복막투석 환자의 사망률이 높은 정확한 이유는 밝혀져 않다. 혈액투석 환자에서처럼 복막투석 환자에서 저알부민혈증 및 염증반응이 심혈관계 질환 위험인자 인지 알아보고자 하였다. 방 법: 비당뇨병성 말기 신부전, 지속성 외래 복막투석 환자 93명의 심혈관계 질환 위험인자들과 복막 투과 특성 및 알부민, CRP 등의 염증지표에 따라 고해상도 초음파를 이용한 경동맥내막-중간막 두께, 내막-중간막 면적, 플라크의 유무 등을 단면적으로 비교하였다. 결 과: 복막 투과 특성은 고투과막군 14명, 중간 고투과막군 38명, 중간 저투과막군 31명, 저투과막군 10명으로 각 군간의 경동맥 내막-중간막 두께, 플라크 유무 및 내막-중간막 면적의 차이는 없었다. 기존 관상동맥 질환 환자는 8예(8.6%)로 내막-중간막 두께는 관상동맥 질환이 없는 환자보다 증가되었고(0.79±0.21 vs 0.60±0.11 mm, p<0.05) 플라크 형성은 더 많이 보이나 통계적 유의성은 없었다(75.0 vs 63.5%, p>0.05). 혈청 알부민치와 경동맥 내막-중간막 면적은 역상관관계를 보였다(r=-0.27, p<0.05). 저알부민혈증 환자(혈청 알부민 <3.5 g/dL)는 정상 알부민혈증 환자보다 내막-중간막 두께가 증가되었고(0.67±0.15 vs 0.61±0.12 mm, p<0.05), 플라크도 유의하게 많았다(68.0 vs 55.8%, p<0.05). 혈청 CRP치와 경동맥 내막-중간막 면적은 양의 상관관계가 있었으며(r=0.21, p<0.05) CRP가 높은 환자(≥0.8 mg/dL, n=18)에서플라크가 많이 관찰되었으나(65.8 vs 50.0%, p<0.05) 경동맥 내막-중간막 두께 및 내막-중간막면적은 양군간에 차이가 없었다. 다변량 회귀 분석상 경동맥 내막-중간막 두께에 영향을 미치는 인자는 고령, 높은 CRP, 기존 심혈관계질환, 낮은 혈청 알부민 순이였다. 결 론 : 복막투석 환자에서 고해상도 초음파를 이용한 경동맥 내막-중간막 두께 측정은 심혈관계 질환 유무 예측의 비침습적 검사가 될 수 있고 저알부민혈증 및 높은 CRP과 같은 만성염증 지속상태가 복막투석 환자에서 죽상 동맥 경화증의 발생을 증가시킨다고 가정할 수 있다. Background : Continuous ambulatory peritoneal dialysis(CAPD) patients with low albumin(LA) and signs of inflammation reflected by increased C-reactive protein(CRP) level have an increased mortality, but the mechanism of this phenomenon is not clear yet. Methods : To answer whether LA and inflammation also enhance cardiovascular risk in CAPD patients, we performed cross sectional study measuring carotid artery intima-media thickness(IMT), calculated intima-media area(cIM area) and the presence of plaque by high-resolution B-mode ultrasonography in 93 non-diabetic CAPD patients. Results : Patients with coronary artery disease (CAD, n=8) had significantly increased IMT(0.79± 0.21 mm vs. 0.60±0.11 mm, p<0.05) and higher prevalence of carotid plaques(75.0% vs. 63.5%) compared to the non-CAD patients. Significant inverse correlation was observed between serum albumin (SA) level and cIM area(r=-0.27, p<0.05). Those patients with LA(SA <3.5 g/dL) had significantly increased IMT compared to non-LA patients(0.67±0.15 mm vs 0.61±0.12 mm, p<0.05). Prevalence of carotid plaques was also significantly higher in LA patients (68.0% vs. 55.8%, p<0.05). CRP level revealed a significant positive correlation with cIM area(r=0.21, p< 0.05). Patients with high CRP(≥0.8 mg/dL, n=18) had higher prevalence of carotid plaques (65.8% vs. 50.0%, p<0.05) compared to those patients with CRP <0.8 mg/dL, but IMT and cIMT area were not different. By multivariate logistic regression analysis, old age, high CRP, history of CAD and low SA were the independent risk factors affecting IMT. Conclusion : Our study strongly suggests that low albumin and chronic inflammatory state of CAPD patients could be associated with increasing atherosclerotic cardiovascular disease. (Korean J Nephrol 2002;21(2):285-294)

원발성 간암종의 조직 발생에 대한 면역조직학적 연구

강대영,최정목,송규상,이헌영 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

To assess the utility of cytokeratin(CK) profile in the differential diagnosis of primary liver carcinomas(PLCs),we evaluated a series of needle biopsied specimens and surgically resected PLCs, comprising 30 pure hepatocellular carcinomas(HCCs) and 20 cholangiocarcinomas(CCs). The results are summarized as follows: 1) The pure hepatocellular carcinomas showed intense cytoplasmic expression of anti-cytokeratin (AE1/AE3);28/30 (93.3%). 2) The cholangiocarcinomas showed cytoplasmic expression of 2 types of CK(Anti-CK19,AE1/AE 3);14 of 20 (70.0%) of the CCs evidenced mature bile duct phenotype (CK 19), whereas 10 of 20 (50.0%) displayed the hepatocytic profile(AEl/AE3). 3) Nine of 30 HCCs also displayed the bile duct cell profile; high grade HCCs(8/20), and low grade HCCs(1/10). The above results show that a mixed biological phenotype can be found both among morphologically pure HCCs and peripheral CCs, suggesting that these two forms could share a common histogenesis.

혈액투석중인 만성신부전 환자에서 골대사 지표로써의 Osteocalcin치

송치운,이진홍,안미애,윤환중,윤상임,성기양,이강현,송민호,이강욱,신영태,김영건,노흥규 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.2

Background : Serum osteocalcin is synthesized by osteoblast and has been shown to be sensitive indicator of bone turnover inpatients with various metabolic bone disease. In renal osteodystrophy, serum osteocalcin is elevated due to decreased renal clearance and elevated level of PTH. This study was done to evaluate the usefulness of serum osteocalcin as a marker of bone metabolism and the correlation with other biochemical markers of bone metabolism. Methods : We measured serum osteocalcin, calcium, phosphorus, ALP(alkaline phosphatase) and PTH(parathyroid hormone) in 37 patients with end stage renal disease on hemodialysis. Osteocalcin was determined by radioimmunoassay and PTH was determined by radioimmunometric assay. Results : 1) The mean level of serum osteocalcin in ESRD patients was 233.8± 218.2ng/ml which was significantly higher than that of controls(p<0.0001). 2) The mean level of serum PTH in ESRD patients was 40.5± 43.8pg/ml was significantly higher than that of controls(p<0.005). 3) There was a significant positive correlation between the level of serum PTH, ALP and the level of serum osteocalcin in ESRD patients. 4) By using multiple regression, PTH is most reliable factor that affect to elevated level of serum osteocalcin ( beta coefficient = 0.687, Sig T<0.05). Conclusion : Serum osteocalcin as a marker of bone metabolism in ESRD patients is more useful than other biochemical marker such as serum calcium, phosphorus, ALP and PTH is a most reliable factor that affect to elevated level of serum osteocalin.

乳房 疾患의 病理組織學的 硏究

姜大榮,宋圭祥 충남대학교 의과대학 지역사회의학연구소 1985 충남의대잡지 Vol.12 No.2

A histopathologic study was made on 844 cases of the breast lesions of female, which were collected, from Jan. 1980 to July 1985, at the Department of Pathology, School of Medicine, Chungnam National University. The results were as follows 1. Among 844 cases of the breast lesions of female the age distribution shows the highest frequency in the third decade(317 cases, 37.6%), followed by the 4th decade(260 cases, 30.8%), the 5th decade(147 cases, 17.4%), the 6th decade(53 cases, 6.3%), and the second decade(42 cases, 5.0%) in decreasing order of frequency. 2. The most: frequent breast lesion was the nonneoplastic lesion(345 cases, 40.9%), followed by benign tumor(326 cases, (38.6%), malignant tumor(130 cases, 15.4%), and inflammatory disease(43 cases, 5.1%). 3. Among inflammatory disease, acute mastitis and abscess was 21 cases and chronic mastitis and plasma cell mastitis was 20 cases. Seventeen cases of inflammatory disease were present in the third decade. 4. The most prevalent nonneoplastic lesion was fibrocystic disease(297 cases, 86.1%), which reveals the highest frequency in the fourth decade(129 cases), followed by third decade(93 cases) and the fifth decade(54 cases). The mean age of fibrocystic disease was 32.9 years. 5. Among benign tumor fibroadenoma was 269 cases (82.5%), intraductal papilloma and lactating adenoma, 17 cases, respectively, lipoma 7 cases, and tubular adenoma, cystosarcoma phyllodes, fibroma, 4 cases, each. The most prevalent age of fibroadenoma was the third decade(the mean age; 27.7 years). 6. Among 130 cases of malignant tumor infiltrating duct carcinoma shows the highe incidence(112 cases, 86.1%), followed by noninfiltrating duct carcinoma 10 cases, lobula carcinoma 4 cases, and sarcoma 3 cases, in decreasing order of frequency. The most frequen subtype of infiltrating duct carcinoma was simple type, followed by scirrhous, medullary, papillary, colloid, and infiltrating comedocarcinoma. The malignant tumor shows highest incidence in the fifth decade and the mean age was 47.6 years.

포타슘과 소디움이 白鼠腎臟 動脈에 미치는 影響에 對한 病理學的 硏究

宋圭祥,姜大榮 충남대학교 의과대학 지역사회의학연구소 1986 충남의대잡지 Vol.13 No.1

There are experimental and epidemiological data which indicate that high sodium intake is a major factor in the genesis of hypertension and that potassium moderates salt induced hypertension. To study the effect on blood pressure and morphologic change of sodium and potassium intake, the male Sprague-Dawley rats were fed diets containing different amount of sodium(4%, 8%) and potassium(1%, 2%, 4%) for six months. The systolic blood pressure was measured monthly from the first month, and the size and morphologic change of renal arteries were also investigated monthly from the second month of feeding. The results were as follows: 1. High sodium intake induces hypertension, and in the case of feeding the same sodium concentration, hypertensinogenic effect is greater in the rats receiving less dietary potassium intake than high potassium intake. 2. There is more statistically significant increase of ED/LD ratio in the high sodium and potassium fed groups than those of the control group (P<0.05). Medium sodium and potassium fed groups also reveal statistical significance(P<0.05). 3. Hyperplasia of endothelial cells and hypertrophy of media are more prominent in the high sodium fed groups than those of the control group, and these changes are more evident in the high sodium intake groups than those of the medium sodium intake groups. In the groups receiving the same sodium concentration, each blood vessel reveals the decrease of ED/LD ratio with increment of potassium concentration, but there is no statistical significance (P>0.05).

간생검조직의 병리조직학적 연구

송규상,강대영 충남대학교 의과대학 지역사회의학연구소 1986 충남의대잡지 Vol.13 No.2

A histopathologic study was performed on 158 liver biopsies submitted to the department of pathology, Chungnam National University College of Medicine, during the period from Jan. 1982 to Oct. 1986, and the results were as follows : 1. Amont 158 liver biopsies, the hepatitis is the most frequent(98 cases, 62.0%), followed by fatty liver(24 cases, 15.2%) liver cirrhosis(20 cases, 12.7%), and hepatoma(6 cases, 3.8%) The overall sex ratio was 3.4:1. 2. Among 98 cases of hepatitis, chronic hepatitis was 74 cases, nonspecific reactive hepatitis 12 cases, alcoholic hepatitis 5 cases, acute hepatitis 5 cases, and neonatal hepatitis 2 cases. The sex ratio of chronic hepatitis was 4.7 : 1 with the average age of 37.1 year-old and the prevalent age in the fourth decade. Chronic active hepatitis was to times more than chronicc persistant hepatitis. 3. The sex ratio of fatty liver was 2:1, the average age 37 year old, and the prevalent age was the fourth decable. 4. Liver cirrhosis was encountered in 20 cases with the sex ratio of 4:1, the average age of 35.5 year-old, and the prevalent age in the fifth decade. 5. The average age of hepatoma was 42.8 years old and the sex ratio was 2:1.

갑상선 유두상 암종과 유두상 증식에 대한 S-100 단백 및 EMA 발현에 대한 연구

강대영,김경희,최정목,송규상,강동욱 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.2

The diagnosis of papillary carcinoma of the thyroid is readily achieved based on a defined aggregate of histopathologic features. A papillary architecture is an imporant but not pivotal component of the diagnosis. The classic nuclear features are the essential diagnostic element. However, both papillary architecture and nuclear features may be encountered in other conditions and produce problems in histopahologic interpretation. A papillary architecture may be found within a follicular or an oncocytic neoplasm of within the nodules of hyperplastic nodular goiter. Moreover, there may be scattered cells within several thyroid lesions that display some of the nuclear characteristics of papillary carcinoma. The distinction of these lesions from papillary carcinoma is important for differential diagnosis. Thus, the availability of supportive diagnosic evidence would be helpful. Immunoreactivity for S-100 and epithelial membrace antigen was investigated in 16 papillary thyroid carcinomas(PTC), including three examples of the follicular variant, 16 cases of papillary hyperplasis, 8 follicular adenomas, and 6 follicular carcinomas. The degree of reaction was rated on a four point scale: +++, most cells(>51%) have positive staining; ++, 11-50% of cells have positive staining; +, less than 10% of cells have positive staining; -, no positive staining is present. The results are summarized as follows: 1) All the papillary carcinomas showed intense nuclear and cytoplasmic expression of S-100 (+++; 7 cases, ++; 9 cases), and EMA was expressed(++; 7 cases, ++; 6 cases, +; 3 cases). 2) In the cases of papillary hperplasia, papillary foci were generally negative for S-100 and EMA(12/16 ; -). In some areas, they were focal, of weak intensity, and showed preferential apical cytoplasmic location of oncocytic change. 3) Four of eight follicular adenomas expressed S-100(++;2 cases, +; 2 cases), whereas four were completely negative. EMA was also expressed(++; 2 cases, +; 3 cases). 4) Follicular carcinoma showed variable expression of S100 and EMA Five of six follicular carcinomas expressed S-100(++; 2 cases, +; 3 cases), whereas one was negative. EMA was also expressed(++; 2 cases, +; 1 case). In conclusion, in the distinction of papilary carcinoma from papillary hyperplasia, we would recommend an initial histological appraisal, with immunohistochemical staining of S100 and EMA in difficult cases.

말기 신부전 환자에서 투여 경로에 따른 유전자 재조합 인 Erythropoietin(Epokine^(�))의 효과와 안정성에 대한 연구 : 제 4상 연구 A Phase 4 Single Center Study

송현용,윤향숙,강신욱,최규헌,하성규,이호영,한대석 대한신장학회 2002 Kidney Research and Clinical Practice Vol.21 No.2

목 적 : 국내 독자적 기술로 첫 개발된 유전자 재조합 인 에리트로포에틴(Epokineⓡ)의 투여방법에 따른 유효성과 안정성을 알아보고자 하였다. 방 법 : Epokineⓡ을 주당 100-150 U/kg, 주 2회, 12주간 피하 주사하여 목표 또는 안정된 혈색소치 유지 후 무작위로 피하 및 정맥 주사군으로 나누어 12주간 혈액투석 및 복막투석 환자13명, 28명에서 유효성, 안정성을 분석하였다. 결 과 : 양군에서 혈색소치와 헤마토크릿은 Epokineⓡ 투여 2주 후부터 증가, 혈색소치가 혈액투석 환자는 투여 전 7.3 g/dL, 투여 12주 9.5 g/dL, 24주 10.6 g/dL로, 복막투석 환자는 6.8g/dL에서 12주 10.2 g/dL, 24주 10.8 g/dL로 증가하였다. 양군에서 교정 망상적혈구수(%)는투여 1주 후부터 전 기간동안 증가하였다. 혈청 내 철분, ferritin치는 양군에서 투여 2주 후부터감소, 총 철분 결합능은 투여 2주 후부터 증가하였다. 12주째 혈액투석 환자에서 많은 Epokineⓡ용량이 필요했으나(142.2±20.5 vs 117.3±33.6 U/kg/wk, p<0.001) 24주 후 양군의 용량차이는 없었다(123.6±41.5 vs 99.2±49.3 U/kg/wk, p>0.05). 혈액투석 환자에서 피하 주사가Epokineⓡ 투여용량이 더 적었으나(97.4±15.4 vs 145.4±2.9 U/kg/wk, p<0.002) 복막투석 환자는 차이가 없었다(93.0±60.2 vs 105.4±9.7 U/kg/wk, p>0.05) 연구기간 중 9명(18.8%)에서혈압강하제 추가가 필요한 혈압상승, 근육통 동반 상기도 감염 유사증상 및 두통 등의 부작용이 있었다. 대부분 자연소실 혹은 대증요법으로 치유되었고 2명이 부작용으로 탈락하였다. 결 론 : 말기 신부전 환자의 빈혈 개선에 사용하는 Epokineⓡ의 효과는 복막투석 환자에서 더 좋았으며 투여 경로는 정맥주사보다 피하주사가 높음을 알 수 있었다. Background : We evaluated the clinical efficacy and safety of recombinant human erythropoietin(Epokine^(ⓡ)). Methods : A comparative prospective study of intravenously and subcutaneously administrated Epokine^(ⓡ) conducted 13 patients performing hemodialysis and 28 patients performing continuous ambulatory peritoneal dialysis with end-stage renal disease. Epokine^(ⓡ) was given initially at a dosage of 100 unit/kg, subcutaneously, two times a week. The patients had achieved stable or more than 10 g/dL of hemoglobin level for 12 weeks and then we randomized switching intravenously or subcutaneously administrated Epokine^(ⓡ) for another 12 weeks. Results : Hemoglobin(g/dL) and hematocrit(%) increased significantly from baseline levels beginning from 2 weeks after Epokine^(ⓡ) administration. In HD patients, hemoglobin increased significantly from 7.3 to 9.5 after 12 weeks and to 10.6 after 24 weeks. In CAPD patients, hemoglobin increased significantly from 6.8 to 10.2 after 12 weeks and then 10.8 after 24 weeks(p<0.05). Corrected reticulocyte count(%) was significantly increased from baseline levels beginning from 1 week after Epokine^(ⓡ) administration and continuously increased during study period in both group. Serum ferritin and serum iron decreased significantly and total iron binding capacity increased significantly after 2 weeks. At 12 weeks, HD patients were significantly needed more dosage of Epokine^(ⓡ) than CAPD patients(142.2±20.5 vs 117.3±33.6 U/kg/wk, p<0.001), but at 24 weeks, the dosage was not different(123.6±41.5 vs 99.2±49.3 U/kg/wk, p> 0.05). In HD patients, intravenously administrated Epokine^(ⓡ) group was more dosage than subcutaneously group(97.4±15.4 vs 145.4±2.9 U/kg/wk, p< 0.002), but CAPD patients were not different by administration method(93.0±60.2 vs 105.4±9.7 U/kg/ wk, p>0.05). The 9 cases(18.8%) were suffered from headache and flu-like syndrome, but these side effects were not severe and disappeared from conventional therapy. Conclusion : Epokine^(ⓡ) administration is safe and effective in treating anemia of ESRD patients and subcutaneously administration is significantly more effective than intravenously. (Korean J Nephrol 2002;21(2):190-198)

복막유착의 원인 규명과 예방을 위한 실험적 연구

강대영,송규상,노승무,조준식,이태용 충남대학교 의과대학 지역사회의학연구소 1999 충남의대잡지 Vol.26 No.1

Postoperative peritoneal adhesion is the most common cause of intestinal obstruction, and intestinal obstruction is one of the most difficult problems in abdominal surgery. In this experimental study for developing peritoneal adhesion model and its prevention, we divided rats into 5 groups; the talc powder treated group, the cotton-fluff treated group, the talc+fluff treated group, the ischemia group and the control group. There were 16 rats in each group. We made incisions on the midabdomen of the rats and administered talc powder, cotton fluff and talc powder+fluff into the peritoneal cavity respectively. In the ischemia group, we clamped the root of superior mesenteric artery for two minutes to induce ischemia on the small bowel of the rat. We counted the number of the peritoneal adhesive sites in peritoneal cavity on the 7th, 14th , 21st and 42nd postoperative day after peritoneal adhesion induced operation, and observed the characters of the adhesion state. The average number of the peritoneal adhesions were 1.25 per one rat in the talc powder group, 1.44 in the fluff group, 1.31 in the talc+fluff group and 1.31 in the ischemia group compared with 0.94 per one rat in the control group performed sham operation. 91.6% of the all adhesions were observed on the peritoneum of the previous incision sites and only 8.4% of the adhesions were adhered to the peritoneal surface of non-incision sites. The data of this study suggest that peritoneal injury and inflammatory reaction is the major cause of peritoneal adhesion. Also this study indicates that foreign bodies can develop peritoneal adhesion, but the effect is much lower than that of peritoneal injury with inflammatoty reaction.