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신현진(HJ Shin),조오숙(OS Cho),유범준(BJ Yoo),이상범(SB Lee),임헌정(HJ lm),송장신(JS Song) 대한산부인과학회 1994 Obstetrics & Gynecology Science Vol.37 No.3
Congenital cystic adenomatoid malformation of the lung (CCAML) is rare pulmonary cystic disease. CCAML has benn detected in prematurity, stillborn and respiratory distressed infant or child by chest X-ray or CT scan. Few cases of antenatal diagnosis of this malformation has been reported. We experienced a case of CCAML which was detected ultrasonographically at 22 weeks of gestation and present thise case with review of related literatures.
SH Song,JG Kim,HJ Song,B Mohana Kumar,SR Cho,CY Choe,SH Choi,노규진,SY Choe 사단법인 한국동물생명공학회 2005 Reproductive & developmental biology Vol.29 No.2
The objective of this study was to examine the effect of EGF on meiotic maturation and pronuclear (PN)formation of porcine oocytes. Prepubertal gilt cumulus-oocyte-complexes (COCs) aspirated from 2 6 mm folliclesof abbatoir ovaries were matured in TCM199 containing 0.1 mg/ml cysteine, 0.5 ug/ml FSH and LH, and EGF (0,5, 10, 20, 40 ng/ml) for 22 hr at 39°C in a humidified atmosphere of 5% CO2 in air. They were then cultured foran additional 22 hr without hormones. In Experiment 1, to examine the nuclear maturation at 44 hr of culture, theexpanded cumulus cells were removed by vortexing for 1 min in 3 mg/ml hyaluronidase. The oocytes were fixedin acetic acid : methanol (1:3, v/v) at least for 48 hr and stained with 1% orcein solution for 5 min. Nuclear statuswas classified as germinal vesicle (GV), germinal vesicle breakdown (GVBD), prophase-metaphase I (PI-MI), andPII-MII under microscope. In Experiment 2, to investigate PN formation, oocytes were fertilized with Percoll-treatedfreshly ejaculated sperm (1 × 105 cells/ml) in mTBM with 0.3% BSA and 2 mM caffeine for 5 hr, and cultured inNCSU-23 medium with 0.4% BSA. At 6 hr of culture, the embryos were fixed in 3.7% formaldehyde for 48 hr andstained with 10 ug/ml propidium iodide for 30 min. PN status was classified as no or one PN (unfertilized), 2 PN(normal fertilized) and 3 PN (polyspermy). Differences between groups were analyzed ≥ using one-way ANOVAafter arc-sine transformation of the proportional data. The rate of oocytes that had reached to PII-MII weresignificantly (P<0.05) higher in all groups added EGF than that of non-treated group (67%), but it did not differamong the all added groups (86%, 85%, 79% and 81%, in 5, 10, 20 and 40 ng/ml EGF, respectively). No differenceson the incidence of 2PN were observed in all treated groups (25%, 30%, 33%, 29% and 29%, in 0, 5, 10, 20 and 40ng/ml EGF, respectively), however, in non-treated group, polyspermy tended to be increased (66% vs. 58%, 54%, 52%and 55%, 0 vs. 5, 10, 20, 40 ng/ml EGF, respectively). These results suggest that EGF can be effectively used as anadditive for enhancing oocyte maturation and reducing the incidence of polyspermy in pig. (Key words: Pig oocytes, In vitro maturation, Pronuclear formation, EGF)