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      저자 : Sodik Numonov (검색결과 1 건)
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        Nonenzymatic Glucose Biosensor Based on NiNPs/Nafion/graphene Film for Direct Glucose Determination in Human Serum

        Parviz Sukhrobov,Sodik Numonov,Sanshuang Gao,Xamxikamar Mamat,Thomas Wagberg,Yufen Guo,Liwei Liu,Guangzhi Hu 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.7

        This study describes a type of novel nickel nanoparticles (NiNPs) decorated on Nafion-graphene composite film by using the electrochemical deposition method. It was used to fabricate electrochemical biosensors for sensitive nonenzymatic glucose detection. Compared with the Nafion–graphene film and NiNPs-modified glassy carbon electrode (NiNPs-GCE), the NiNPs/Nafion/graphene/GCE showed the best electrocatalytic activity towards glucose oxidation in alkaline medium. The NiNPs/Nafion/graphene/GCE at an applied potential of +0.55V in a linear range of 1–200 μM presented a high sensitivity of 3437.25 μA·mM -1 cm -2 with coefficient of correlation R2 = 0.999; and in a linear range of 200–10 800 μM it performed the best sensitivity of 2848.6 μA·mM -1 cm -2 with coefficient of correlation R2 = 0.995 towards glucose oxidation. For a concentration up to 200 M, a linear range was obtained with a limit of detection of 0.6 μM (signal to noise = 3) and as much as 10 800 μM with a limit of detection of 0.82 μM (signal to noise = 3). The time of responses was about 1–1.5 s with the addition of 0.1–1mM glucose. In addition, NiNPs/Nafion/graphene/GCE also has a high anti-interference ability toward common oxidative interfering species, such as uric acid, ascorbic acid and dopamine. More importantly, NiNPs/Nafion/graphene/GCE was successfully used for the determination of glucose concentration in human serum samples in comparison with a local hospital. The NiNPs/Nafion/graphene/GCE exhibited high sensitivity, low working potential, good stability, excellent electrical properties, enhanced selectivity and fast amperometric responses to glucose oxidation. Thus, as a nonenzymatic sensor, it is promising for future glucose determination development.

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