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Optimization of Extraction Process of Bioactive Compounds from Bene Hull Using Subcritical Water
Rezvan Shaddel,Abdolmajid Maskooki,Mohammad Hossein Haddad-Khodaparast,Sodeif Azadmard-Damirchi,Morteza Mohamadi,Bahram Fathi-Achachlouei 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.5
Bene hull contains antioxidant components. Optimum conditions for bioactive compound extractionprocesses from Bene hull using subcritical water withresponse surface methodology (RSM) were obtained. Temperature (110-200oC), processing time (30-60 min),and the water to Bene hull ratio (10:1-50:1) were theinvestigated factors. The optimal conditions for maximizingthe antioxidant activity were 196.8oC for 52.6 min and aratio of 43.6:1 for water to Bene hull. Under these conditions,the amount of polyphenolic compounds, the reductionpower (RP) (EC50), and the DPPH free radical scavengingactivity (RSA) (EC50) were predicted to be 2,284 mg ofgallic acid/100 g of Bene hull, 0.2002 mg/mL, and 0.6284mg/mL, respectively. HPLC analysis was used to identifythe main phenolic compounds. The subcritical waterextraction technique could be used as a beneficial methodto obtain bioactive compounds from Bene hull.