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      • KCI등재

        문화유산을 활용한 테마파크형박물관에 관한 연구

        박소라(Park, So La),우성호(Woo, Sung Ho) 한국실내디자인학회 2013 한국실내디자인학회논문집 Vol.22 No.6

        The number of visitors to historic sites and museums in Korea has been on a rapid decrease and in order to search for creative ways to work on the problem, the study proposed a theme park-typed museum with cultural heritage applied and reviewed spatial strategies for such theme park-typed museums. Applied methods of research of this study are as follows. First, in order to understand definitions and current situations of cultural heritage use and theme park-typed museums, the study went thoroughly over all sorts of literature and reports as well as findings of advance researches on how to make a use of cultural heritage. Based on the results of the investigations, the study determined characteristics of the theme park-typed museums with cultural heritage applied which the study was proposing and the characteristics were categorized by type. Considering those characteristics and types gained in the study, the study looked for cultural heritage-based theme parks and museums in the world which were good enough to be used as research subjects in the study from the aspects of profitability and level of awareness. The study examined those theme parks and museums in the world and in the end, it found out ways to adopt the findings to the situations in Korea and discussed expecting effects as well. As to the characteristics of the theme park-typed museums with cultural heritage applied, the study divided those characteristics into mutual cooperation, location, communicability, authenticity, maintainability, education, durability, narrativity, undailiness, interactivity and leisure. The types were categorized into ride, environment direction, cartoon character and souvenir by attraction pattern. When cultural heritage is used actively, it would improve competitiveness of historic cultural sites and museums in return, making people realize the iterative structure of excavation, conservation, maintenance and use of cultural heritage. That would create many kinds of added values, re-discovering culture of the country. At the same time, it would also create a new value of culture as well. Now, it is important for us to do harder with researches on how to evolve museums and exhibition spaces. Considering that, the study is believed to make a contribution to revival of historic sites and museums in Korea but also establishment of scientific strategies.

      • Effect of ER signal peptides (KDEL) on expression and function of monoclonal antibody in plant

        Da-Young Park,Eun-Yi Oh,Zhe Lu,Yangkang So,Mi-Hyun Ahn,Mira Song,Kyung-Jin Lee,Doo-Byoung Oh,Youngkwan Kim,Kisung Ko 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1

        Plant genetic engineering led to the production of plant-derived mAb (mAbP), which provides a safe and economically feasible alternative to the current animal expression systems. In this study, the expression level of mAbP SO57 between with and without Lys-Asp-Glu-Leu (KDEL) was compared in transgenic plant. PCR and PT-PCR analyse showed stable gene insertion transcription of heavy and light chain genes of mAbP SO57 with or without KDEL in plant, respectively. We validated expression of mAbP SO57 by western blot. Western blot showed the significantly higher expression level of mAbP SO57 with KDEL compared to without KDEL. Flow cytometry (FACS) analysis showed that the Fc domains of both purified mAbP and mammalian-derived mAb (mAbM) evidenced similar binding activity to the FcγRI receptor (CD64). High performance liquid chromatography (HPLC) analysed, glycosylation patterns of mAbP SO57 with or without KDEL. The mAbP SO57 with KDEL had glycan profile with both oligomannose type (47.6%) and golgi type (52.4%), while the mAbP SO57 without KDEL had only golgi type (100%) glycans. Neutralizing analysis with rabies virus CVS-11 showed the similar neutralizing activity between mAbP SO57 with and without KDEL. These results suggest the potential of mAbP SO57 for rabies immunotherapy, regardless of plant specific glycan structures.

      • KCI등재

        은행나무의 잎끝마름병에 미치는 SO2 의 영향

        이두형,배공영,박소홍,강연지 한국환경농학회 1999 한국환경농학회지 Vol.18 No.2

        대기오염과 병충해발생과의 관계를 규명하고자 은행나무에 SO₂가스를 처리한 후, 잎끝마름병균을 접종하여 식물의 생리·생화학적인 변화를 조사하였다. 0.1㎕/ℓ의 SO₂가스를 하루 8시간씩 7일간 은행나무에 처리한 결과 Chlorosis등의 가시피해는 나타나지 않았으나, 광합성이 대조구에 비해 40%가량 감소되고, CO₂이용효율과 수분이용효율이 감소되는 현상을 보였다. 가스처리 후 병원균을 상처접종시키고 42일 뒤 SI와 SFI의 발병도를 조사한 결과 SI가 SFI에 비해 3배 가량 빠르게 병이 진전되어 있었다. 병원균의 먹이가 되는 당의 함량은 대조구, SFI, SI간에 유의성을 보일 만큼의 차이는 보이지 않았으나, 광합성은 SI가 SFI에 비해 11%가량 저하되었다. 광합성이 저하되었음에도 불구하고 당의 함량이 유지되었으므로 탄소 고정관계를 알아본 결과 CO₂ 이용효율이 저하되지 않았음을 알 수 있었다. 이와 함께 수분이용효율이 SI에서 SFI에 비해 13.2% 증가되었다. 이상의 결과를 종합해 보면, 이온유출량 등으로 보아 SO₂가스처리로 인해 식물이 연약해져 병원균에 대한 저항력이 낮아졌고, 수분이용효율의 증가로 인해 탄소 고정이 활발하게 이루어짐으로써 당이 원활히 합성되는 한편 내부적인 변화로 인해 합성된 당의 전류가 억제되어 잎에 당이 축적되었기 때문에 SI의 발병도가 훨씬 높았던 것으로 생각된다. To examine the relationship between air pollution and occurrence of disease in plants, we investigated the alteration of physiology and biochemistry of Ginkgo biloba by inoculating with Coniothyrium sp. and fumigating with 0.1㎕/ℓSO_(-2) Visual damage did not appear but photosynthesis, CO₂use efficiency(CUE) and water use efficiency(WUE) were reduced when G. biloba was exposed to SO₂for 7days (8 hours a day). When inoculated with Coniothyrium sp., the seventies of damage in G. biloba showed a threefold difference between SO₂-treatment (SI) and SO₂-free treatment (SFI) at day 42 from initial inoculation. Little difference was observed in sugar contents that may be used pathogens feed, among control, SFI and SI. In spite of the reduction in photosynthetic rate, sugar contents and CUE were maintained. WUE was enhanced 13% more at SI than SFI. The CO₂fixation boosted because of enhanced WUE, and thus sugar synthesis was not affected. In addition, sugar transport seems to be retarded for some internal alteration. Consequently, the severity of SI was more serious than that of SFI because Coniothyrium sp. easily invades into the physical texture of G. biloba weakened by SO₂fumigation and because sugar was accumulated in leaves of G. biloba.

      • KCI등재

        도시근린공원 방범설계 (CPTED)의 효과성 검증

        홍소연(Hong, So Yeon),권순호(Kwon, Soom Ho),박세희(Park, Se Hee),김은기(Kim, Eun Kee) 한국공안행정학회 2020 한국공안행정학회보 Vol.29 No.1

        이 연구는 도시근린공원에서의 범죄예방환경설계 효과성 검증을 목적으로 실시되었다. 연구목적 달성을 위해 선행연구에서 활용된 범죄예방환경설계 평가항목을 활용하여 대상 공원 두 곳의 범죄예방환경설계 수준을 객관적으로 평가하였고, 설계 수준이 우수한 공원과 낮은 공원 방문객들을 대상으로 각각 범죄피해두려움을 측정하였다. 분석결과 설계 수준이 우수한 공원보다 설계 수준이 낮은 공원에서 방문객들의 범죄피해두려움이 더 높게 나타남을 확인 할 수 있었으며, 두 공원에서 느끼는 범죄피해두려움의 평균차이는 통계적으로 유의한 차이로 확인되었다. 따라서 도시근린공원에서의 범죄예방환경설계는 이용객들의 범죄피해 두려움을 낮추는 효과가 있다고 할 수 있다. 그러나 이 연구는 범죄피해두려움에 영향을 미치는 다른 요인들을 통제하지 못하였다는 점, 조사대상 공원의 범죄예방환경설계 수준에 대한 평가의 정교성이 다소 낮다는 점 등의 한계가 존재한다. 그럼에도 불구하고 ‘CPTED 인식’과 범죄피해두려움의 관계를 주로 검토하여 효과성을 검증하고자 한 기존 선행연구와는 달리 객관적 평가척도를 활용하여 설계수준 차이에 따른 범죄피해 두려움의 차이를 검증함으로써 CPTED의 효과성을 검증하였다는 점에서 의의가 있는 연구라 하겠다. This study was conducted to verify the effectiveness of crime prevention environment design in urban neighborhood parks. To achieve the objectives of the research, the crime prevention environmental design evaluation items used in the previous studies were objectively evaluated, and the crime prevention environment design levels of two target parks were objectively evaluated. Fear of crime was measured. As a result, visitors were found to have higher fears of crime victimization in parks with lower design level than those with better design level, and the average difference between crime fears felt between two parks was statistically significant. Therefore, the design of crime prevention environment in urban neighborhood parks can be said to reduce the fear of crime victims. However, this study has limitations such as the lack of control of other factors affecting the fear of crime, and the low level of elaboration of the assessment of crime prevention environment design of the park under investigation. Nevertheless, unlike previous studies that mainly examined the relationship between CPTED awareness and fear of crime, CPTED by verifying the difference of crime damage fear according to the design level by using objective evaluation scale. It is a meaningful study in that it verified the effectiveness of.

      • KCI등재

        재량적 발생액과 부채조달비용 간의 관계에 대한 실증적 증거

        박종일 ( Jong Il Park ),윤소라 ( So Ra Yoon ) 한국회계학회 2014 회계학연구 Vol.39 No.3

        본 논문의 목적은 재량적 발생액과 부채조달비용 간에 어떤 관계가 있는지를 규명하는데 있다. 선행연구들은 경영자가 기회주의적 이익조정 수단의 하나로 재량적 발생액( DA) 수준을 증가시키면 채권투자자들은 대출이자율을 증가시킬 것으로 예상하였다. 그러나 이러한 주장은 채권투자자들이 기업의 DA수준에 대하여 효과적인 탐지가 가능할 것이라는 전제가 있어야 한다. 따라서 채권투자자 관점의 논의이다. 하지만 이와 상반되게 경영자 관점에서는 DA와 부채조달비용 간에 유의한 음(-)의 관계가 나타나야만 경영자는 부채조달비용을 낮추기 위한 동기로 DA를 이용할 경제적 유인이 발생될 수 있다. 본 연구는 부채차입이자율 스프레드로 측정된 부채조달비용( YS)과 DA자체 변수 간에는 어떤 관계가 있는지를 살펴보는데 있어 이전 연구범위를 확장시켜 검증해 보았다. 본 연구에서는 두 변수 간의 관계에 대한 일반화 가능성을 제고하기 위하여 회사채를 발행한 기업뿐만 아니라 회사채를 발행하지 않은 기업에 대해서도 비교분석을 수행했다. 분석기간은 2001년부터 2011년까지이며, 표본은 금융업을 제외한 12월 결산법인으로 최종 10,479개 기업/연 자료가 분석에 이용되었다. 실증분석 결과에 따르면 첫째, 부A자체와 YS간에 1% 수준에서 유의한 음(-)의 관계가 있는 것으로 나타났다. 이 관계는 회사채를 발행한 기업들보다는 주로 회사채를 발행하지 않은 기업들에서 기인된 결과인 것으로 나타났다. 둘째, 선행연구와 달리 부채차입이자율 스프레드를 이용하여 분석하면 회사채를 발행한 기업의 경우 DA와 부채조달비용 간에는 대체로 유의한 관계가 나타나지 않았다. 셋째, Prevost et al.(2008)의 방법에 따라 DA와 비투자등급의 상호작용변수와 YS간의 관계를 살펴본 결과, 채권투자자들이 DA를 직접 탐지하지 못할 뿐 아니라, 비투자등급의 정보위험이 DA를 대리하는 매개변수의 역할을 한다는 Prevost et al.(2008)의 주장은 관찰되지 않았다. 따라서 본 연구결과는 상장기업의 경영자들이 부채차입시에 부채조달비용을 낮추기 위한 동기로 DA를 이용하려는 경제적 유인이 존재한다는 것을 실증적 증거로서 보여주었다는데 의의가 있다. 이와 더불어 본 연구의 발견은 관련연구에 추가적인 공헌을 할 것으로 기대된다. The purpose of this study is to examine the relationship between discretionary accruals and cost of debt financing. Earnings management studies are common in accounting literature. Theory and evidence indicate that managers` concerns over current performance motivate them to engage in boosting reported earnings. Analysis of earnings management often focuses on management`s use of discretionary accruals. Managers can exercise discretion over accrual choices that are allowed under GAAP in order to reach a desired level of earnings. This is, firms` management manages earnings because they have some incentives to do so. However, earnings management hides a true performance of a firm from debtholders and other external parties. Earnings management also distorts quality of reported earnings, and quality of accounting information impacts debtholders` estimates of future cash flows fromwhich debt repayments will be served. As a result, opportunistic earnings management activities increase information asymmetries between managers and outside investors with respect to a firm`s true underlying performance, which in turn increase information risk faced by outside investors. Especially, debtholders have contractually fixed claims such as periodic interest payments. They tend to focus on future cash flows to ensure a firm`s ability to pay interest and principal. In addition, unless debtholders can "see through" and undo earnings management, their resource allocation decisions are potentially affected in a negative way. In that regard, examining the effect of opportunistic earnings management in the debt market is important. Prior studies expected that debtholders increase interest rates on firm`s debts when managers increase discretionary accruals ( DA) for the purpose of opportunistic earnings management. This expectation is coming from debtholders` point of view, because it is based on the assumption that rational debtholders can effectively see through a firm`s level of discretionary accruals. Then, prior papers assert that rational debtholders are able to see through managers` attempts to opportunistically influence earnings perceptions and penalize firms for doing so by demanding a higher rate of return. However, from manager`s point of view, we should observe the negative relationship between discretionary accruals and cost of debt, so we will be able to explain the managers` economic motivation to decrease cost of debt using discretionary accruals. Prior researches provide inconsistent empirical evidence on this relationship. Prevost et al. (2008) cannot find a significant relationship between discretionary accruals and cost of debt measured by bond`s yield spreads, while Park et al. (2011) and Lee and Ko (2012) find a significantly positive association between these two variables. However, these prior studies have several limitations. For example, Park et al. (2011) focus on bond issuing firms only and Lee and Ko (2012) measure discretionary accruals taking absolute value of them. Therefore, this study is meaningful because it extends prior researches and examines the association between cost of debt ( YS) measured by borrowing`s yield spread and the level of discretionary accruals ( DA). This paper analyzes all samples including not only bond issuing firms but also bond unissuing firms, and KOSPI and KOSDAQ listed firms in order to generalize the test results. Sample periods are from 2001 to 2011, and totally 10,479 firm-year samples are used in this research. This paper finds the following results. First, there is a statistically significantly negative association between cost of debt and discretionary accruals at 1% level, even after controlling firms` credit ratings and other variables that can affect firm`s cost of debt. This result is consistent in full samples including KOSPI and KOSDAQ firms, and in each type of stock markets separately. Also, we find the same results no matter whether we use discretionary accruals in Dechow et al. (1995) or performance-adjusted discretionary accruals in Kothari et al. (2005). Meanwhile, this significantly negative association between two variables is coming from bond unissuing firms rather than bond issuing firms. Second, unlike the prior studies, we cannot find a statistically significant relation between cost of debt and discretionary accruals in bond issuing samples when we measure cost of debt using borrowing`s yield spread. This is an inconsistent result with Park et al. (2011). Third, following Prevost et al. (2008), we examine the association between cost of debt financing and interaction term of discretionary accruals and non-investment grade rating (i.e., high default risk firms), but we cannot find that the yield penalty for earnings management is significantly greater for non-investment grade rating, and moreover, debtholders cannot see through the information risk of increasing level of discretionary accruals and managers` attempts of opportunistic earnings management. As a result, we find inconsistent empirical test results with Park et al. (2011) and Lee and Ko (2012). Overall, these findings suggest that debtholders do not see through accrualsbased earnings management. In other words, this evidence suggests that debtholders do not perceive earningsmanagement as opportunistic behavior. Accounting information plays an important role in the capital allocation process. Accordingly, understanding the debt market consequences of earnings management is a relevant and an important issue. In summary, this research implements various analyses to generalize the test result. We includes bond issuing firms and bond unissuing firms, and KOSPI and KOSDAQ firms. We use bare value of discretionary accruals, not just degree of DA, and performance-adjusted DA. We also use a different measurement of cost of debt financing, which is borrowing`s yield spread. With these various approaches, we find that discretionary accruals are statistically significantly negatively associated with cost of debt financing. Therefore, it can support the fact that managers do have economic motivation of opportunistic attempt of earnings management using discretionary accruals in order to reduce cost of debt. This implies that debtholders cannot effectively detect increasing information risk of firms due to the earnings management and they do make a capital allocation decision functionally fixed to reported earnings, although managers increase reported earnings using discretionary accruals in order to reduce cost of debt. Therefore, this study provides several meaningful contributions because it can be an evidence of inefficient allocation of resources in the debt market due to managers` opportunistic attempts of earnings management. This research also contributes to provide useful information regarding earnings management to scholars, practitioners, accounting standard setters, and regulators alike. In addition, researchers who are interested in this area can also apply the discussion in this paper for future studies.

      • KCI등재

        또 하나의 냉전

        박소현(Park So-Hyun) 현대미술사학회 2006 현대미술사연구 Vol.19 No.-

        In 1970, the so-called 'revisionists' of the United States criticised that 'the triumph of American art' was the product of 'cultural Cold War' and Abstract Expressionism as well as the Museum of Modern Art in New York were the weapons of Cold War. Their criticism suggested the problematic issues about modern art in Cold War, but they had some logical limits in their literatures: on the one hand, they were almost interested in the centre of modern art, Paris or New York, and on the other hand, they believed the 'centre', understood as American art in New York after World War Ⅱ, was getting to be diffused into the other areas step by step, for example, Europe in the 1950s, Asia in the 1960s. This means even the critical 'revisionists', probably unconsciously, were not free from and consequently reproduced the geopolitics of the 'centre' with regard to modern art. As for Japan, however, the cultural Cold War' did not come late in the 1960s, but started from the Occupation of the GHQ (General Headquarters/Supreme Commander for Allied Powers) in 1945. And although under the Occupation, the meaning of modern art in Japan was not directly connected to 'the triumph of American art', rather the exhibitions of French modern art were vigorously held. These exhibitions were taken place almost by the newborn 'Kokuritsuhakubutsukan (National Museum)' which had been the property of Tenno (Emperor of Japan) but was reconstituted as that of people by the GHQ on the basis of the Occupational ideology of 'democracy' after the defeat of war. In short, there appeared the 'cultural Cold War' in Japan during the Occupation years in the form of 'democratic modern' French art. And more, the ideology of 'democracy' was so discussive, inconsistent and changing one that could not say that the ever-determined principle of the 'centre' was received as it was without any resistance or modification in Japan. Therefore, I think the case of Japan under the Occupation is a good one to question about the geopolitics of 'centre' reproduced by the 'revisionists'. During the Occupation years, the meaning of 'democracy' and 'democratic modern' art was changed gradually, from anti-militarism (or anti-nationalism) to anti-communism (or anti-socialism) and ended up to the resurrection of Japanese nationalism. With these changes, the French artists or artistic movements was selectively accentuated, from Impressionists to Fauvists or Cubists and to decorative Fauvists, especially Matisse. First, right after the defeat of war, because the Occupational principle was centered on the anti-militarism, 'democracy' was interpreted as a broad one, including socialism, and so there happened a series of competitions for which one is more 'democratic' art between Impressionist realism and socialist realism. But with the opening of 'Kokuritsuhakubutsukan' in 1947, in the second place, the meaning of 'democracy' in that institution was changed into anti-communism, and at the same time 'the autonomy of art(art for art's sake)' was underlined as the institutional principle. On the behalf of this principle, art criticisms and artistic discourses also put it that Fauvists or Cubists was more developed ones than Impressionists because the first was closer to 'the autonomy of art' than the last. This interpretation was conscious of the formulation of modern art by Museum of Modern Art in New York regarded as 'center of art world' and the extraordinary Museum of Modern Art was open within the Kokuritsuhakubutsukan in 1948. And then, from 1949 to 1951, the third aspect of these changes, The GHQ decided to make Japan anti-communist base in Asia, and for the purpose of it to make Japan an independent nation-state was needed. So the Kokuritsuhakubutsukan made a great point of worldliness of Japanese art, and emphasized the superiority of Japanese classics or traditional culture which had been regarded to influence French modern arts. This was the return of Japonism which ha

      • 林地利用에 따른 溪流水의 水質變化

        具瀟瑛,鄭源玉,金弘洙,朴晋源,麻鎬燮 경상대학교 농과대학 부속연습림 2001 演習林硏究報告 Vol.- No.11

        Table 2. Evaluation of stream water quality in three stands by Drinking water quality standard <원문참조> 임지이용에 따른 수질변화 및 오염정도를 구명하여 향후 비점오염원 관리를 위한 기초자료를 제공하고자 경남 진주시 정평리에 위치한 3개 임분(소나무, 밤나무Ⅰ, 반나무Ⅱ임분)에서 2000년 7월부터 9월까지 계류수의 수질변화를 분석하여 얻은 결과는 다음과 같다. 1. 세 임분의 계류수 pH는 소나무 임분(pH6.59)>밤나무 Ⅱ임분(ph 6.53)>밤나무 Ⅰ임분(pH 6.47)계류수 순으로 나타났다. 2. 전기전도도는 밤나무 Ⅰ임분>소나무 임분>밤나무 Ⅱ임분 계류수의 순으로 높게 나타났다. 3. 양이온은 세 임분의 계류수 모두 Ca^2+, Na^+, Mg^2+, K^+, NH_^+의 순으로 높게 나타났다. 4. 음이온은 소나무 임분과 밤나무 Ⅱ임분 계류수에서는 SO_4^2, Cl, NO_3의 순으로 높게 나타났고, 밤나무 Ⅰ임분 계류수에서는 NO_3, SO_4^2, Cl 의 순으로 높게 나타나 차이를 보였다. 5. 소나무, 밤나무 Ⅱ임분 계류수는 pH, NH_4+, NO_3, Cl, SO_4^2이 먹는 물 수질기준 범위내에 있었다. 그러나 밤나무 Ⅰ임분 계류수에서 5항목 중 NO_3는 먹는 물 수질기준을 넘어서는 범위에 있어 임지이용에 따른 하류수질의 오염이 우려되었다. 따라서 이러한 상류지역에서 오염원 차단을 위한 수질정화시설 등의 대책이 마련되어야 할 것으로 사료된다. This study was carried out to clarify the change characteristics of stream water quality by land-use of forest from July to September, 2000 in three stands(Pinus densiflora, Castanea crenata(Ⅰ), Castanea crenata(Ⅱ) stand) of Jeongpyeong-ri, Jinju-si, Gyeongnam. The mean pH of rainfall results in acid rain of 5.3. The pH of stream water in three stands was hugh in order of Pinus densiflora (pH 6.59), Castanea crenata(Ⅱ)(pH 6.53) and Castanea crenata(Ⅰ) stand(pH 6.47). The electrical conductivity of stream water was high in order of Castanea crenata(Ⅰ), Pinus densiflora and Castanea crenata(Ⅱ) stand. Cations of three stand in stream water high in order of Ca^2+, Na^+, Mg^2+, K^+ and NH_4^+. But anions of stream water in Pinus densiflora stand and Castanea crenata(Ⅱ) stand were high in order of SO_4^2, Cl and NO_3. In Castanea crenata(Ⅰ) stand cations of stream water were high in order of NO_3, SO_^2 and Cl. The level of pH, NH_4^+, NO_3, Cl and SO_4^2 of stream water in Pinus densiflora stand and Castanea crenata(Ⅱ) stand reached within the level of domestic use standard for drinking water But the level of NO_3 of stream water in Castanea crenata(Ⅰ) stand was higher than that of domestic use standard. Therefore, non-point sources like forest watersheds which are fertilizer application lands should be taken to the appropriate mitigation measures.

      • Effect of ER signal peptides (KDEL) on expression and function of anti-virus monoclonal antibody in plant

        Da-Young Park,Yangkang So,Kyung-Jin Lee,Zhe Lu,Eun-Yi Oh,Kwang-Wok Min,Seul-Ki Lee,Jung-Hwan Lee,Mi-Hyun Ahn,Mira Song,Doo-Byoung Oh,Youngkwan Kim,Kisung Ko 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1

        Plant genetic engineering has led to the production of plant-derived mAb (mAbP), which provides a safe and economically feasible alternative to the current antibody expression systems. In this study, the expression levels of mAbP SO57 with or without ER-signal peptides (Lys-Asp-Gly-Leu;KDEL) in transgenic tobacco plants were analysed in transgenic plant. PCR and Reverse Transcription-PCR analyses showed existence of heavy and light chain genes of mAb with or without KEDL and their transcription in plant, respectively. Western blot showed that the expression levels of mAbP SO57 with KDEL were significantly higher than that without KDEL. Flow cytometry analysis showed that the Fc domains of both purified mAbP and mammalian-derived mAb have similar binding activity to the FcγRI receptor. High-performance liquid chromatography analysis showed that the mAbP SO57 with KDEL had glycan profile with both oligomannose and golgi type, whereas the mAbP SO57 without KDEL had only golgi type glycans. Neutralizing analysis with rabies virus CVS-11 showed the similar neutralizing activity between mAbP SO57 with and without KDEL. These results suggest that the potential of mAbP SO57 for rabies immunotherapy is regardless of plant specific glycan structures.

      • SCOPUSKCI등재

        아황산에 의한 상치 잎조직의 변색패턴

        이미순(Mie Soon Lee),박소원(So Won Park),이서래(Su Sae Lee) 한국식물학회 1975 Journal of Plant Biology Vol.18 No.1

        Development of a model system for mode of action studies of SO_2 was attempted with a plant tissue. Leaf disks, 1.0㎝ diameter, cut from the lamina of lettuce leaves, were floated on the testing medium and placed in light or dark condition to investigate the discoloration pattern with various sources of SO_2. Discoloration of leaf disks tended to be more serious with higher concentrations of SO_2 and on exposure to the light. Leaf disks were more severely discolored at lower pH with constant SO_2 concentration. These discoloration patterns were highly reproducible and similar in all sources of SO_2. Spectrophotometric evidence suggested that light-mediated discoloration of leaf disks in the presence of SO_2 might occur mainly through chlorophyll α degradation.

      • KCI등재

        한국어 주어일치소에 대한 새로운 접근: 음성형식부 일치 분석

        박소영 ( Park So-young ) 서강대학교 언어정보연구소 2021 언어와 정보 사회 Vol.43 No.-

        This paper presents a PF-based analysis of the so-called subject agreement forms in Korean such as -si- and -tul. These morphemes exhibit the properties that are not consistent with the predictions of syntactic agreement; their realizations are optional, and their overall distributions cannot be fully captured by any syntactic rules. In addition, semantic interpretations are not substantially affected regardless of their presence or absence. Based on these facts, this paper argues that they should rather be taken as agreement forms at PF, thus inserted by the application of a node-sprouting rule at PF. Their status as subject agreement forms will also be refuted, instead generalizing them as agreeing with their C-commanding antecedents being in the same phase.

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