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Adsorption of bovine serum albumin (BSA) on polystyrene (PS) and its acid copolymer
Thidarat Wangkam,Sirasa Yodmongkol,Jarupat Disrattakit,Boonsong Sutapun,Rathasart Amarit,Armote Somboonkaew,Toemsak Srikhirin 한국물리학회 2012 Current Applied Physics Vol.12 No.1
The effect of surface polarity on the adsorption of bovine serum albumin (BSA) on polystyrene (PS), 7%polystyrene-co-maleic anhydride (7%PSMAn) and 50% polystyrene-co-maleic acid (50%PSMA), at pH 7.4,was investigated. Polystyrene represented the non-polar surface while 7%PSMAn and 50%PSMA represented a low and high acid content copolymer. The amount of the adsorbed BSA depended on the amount of the acid content in the copolymer. BSA formed a monolayer with a side-on orientation on the low polarity PS surface, a mixed side-on and end-on orientation on 7%PSMAn and a predominantly sideon orientation on 50%PSMA. The thickness of adsorbed BSA, measured with an atomic force microscope (AFM), varied from 3 nm to 5 nm for the side-on orientation and from 10 nm to 15 nm for the end-on orientation. The average area occupied per BSA molecule was consistent with the proposed orientation,and was 34.8 nm2, 27.8 nm2 and 18.0 nm2 for PS, 7%PSMAn and 50%PSMA, respectively. The adsorption showed a concentration dependency following the Freundlich isotherm, which indicated the interactions among adsorbed BSA molecules on the polymer surface. The adsorption took place as an island-like morphology and started to fuse into a patch-like morphology at higher concentrations before achieving a complete monolayer formation. A non-uniform surface coverage and defects were observed in all cases. It is recommended that for an effective blocking of PS, 7%PSMAn and 50%PSMA, the BSA concentration should be higher than 3 mg/mL.