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RISS 인기검색어
- 검색키워드
- 저자 : Shuaifeng Geng (검색결과 2 건)
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Characterization of Squamosa Promoter Binding Protein-LIKE genes in wheat
Bingnan Wang,Shuaifeng Geng,Di Wang,Nan Feng,Dongdong Zhang,Liang Wu,Chenyang Hao,Xueyong Zhang,Aili Li,Long Mao 한국식물학회 2015 Journal of Plant Biology Vol.58 No.4
Common wheat (Triticum aestivum) is an allohexaploid (AABBDD), with Triticum urartu (AA) and Aegilops tauschii (DD) as its A and D genome donors. Squamosa Promoter Binding Protein-Like (SPL) genes play important roles in plant development; however, a systematic study of this gene family in wheat is still lacking. Here, we mined the wheat genomes for SPLs and found 19 SPL genes each from the T. urartu and Ae. tauschii genomes. We also identified 58 SPL genes from the hexaploid wheat genome. We then compared the conservation and divergence of SPLs between the two diploid species in gene structures, tissuespecific expression patterns and responses to salt stress, drought and low temperature. For functional studies, two hexaploid wheat SPLs, TaSPL3 (with miR156 site mutated) and TaSPL6, were overexpressed in Arabidopsis and found to be involved in flowering time and biomass accumulation. In addition, a mutant allele (TaSPL6-D R ) with a 47 bp duplication upstream of the SBP domain was found to be associated with maturity date and grain number per spike among landraces from a Chinese wheat mini-core collection. Collectively, our work provides useful knowledge on the sequences and functions of wheat SPL genes that may help future functional studies and molecular breeding.
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Lan Zhang,Danmei Liu,Di Wang,Rongzhi Zhang,Shuaifeng Geng,Liang Wu,Aili Li,Long Mao 한국식물학회 2013 Journal of Plant Biology Vol.56 No.2
Seed shattering is one of the major domestication traits of crops. In wheat, except for the Q gene whose mutation renders free threshing and changing of rachis fragility, not much is known about the molecular mechanism for this process. We report here the cloning and characterization of TaqSH1, the ortholog of the rice seed shattering gene qSH1. TaqSH1 encodes a BEL1-like protein that is conserved between monocots and eudicots. TaqSH1 was located on the homoeologous group 3, a potential new genetic locus for seed threshability in wheat. Over expression of TaqSH1 in Arabidopsis resulted in dwarfed plants. The inflorescences of transgenic plants were more compact with larger pedicel angles. Scanning Electron Microscopy (SEM) showed that the transgenic siliques had a narrower replum where the dehiscence zone was altered. In addition, abscission of petals was significantly delayed due to delayed abscission zone development. Real-time PCR assays showed that over expression of TaqSH1 down regulated known Arabidopsis abscission related genes IDA, HAESA, KNAT1/6 and SHP1/2 in the transgenic plants. Taken together, our data suggest that TaqSH1 may represent another example of conserved mechanisms across monocots and eudicots for fruit/grain abscission and should have potential application in genetic manipulation of wheat seed shattering.
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