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      • KCI등재

        Magnetic domain observations of (Tb0.3Dy0.7)Fe1.95 alloys

        Li Kuoshe,Fang Yikun,Zhang Shirong,Yu Dunbo,Han Baoshan 한양대학교 세라믹연구소 2006 Journal of Ceramic Processing Research Vol.7 No.2

        The magnetic domain structures of (Tb0.3Dy0.7)Fe1.95 alloys were investigated using scanning probe atomic and magnetic force microscopies. Results demonstrate that the specimen surface presented corrugated and spiked domain configurations interpreted in terms of surface closure domains in cubic magnetic materials after the specimen was annealed at 673 K for 2 h in an ultra-high vacuum of 5×10−5 Pa. However, a thin soft magnetic Fe layer appeared on the specimen surface after annealing at 723 K for 2 h in a relatively high vacuum of 5×10−3 Pa. As a result, the demagnetized energy in the specimen surface is largely reduced and the interior magnetic domain configurations of the specimen are observed. The results maybe provide an approach for detecting the interior magnetic domain structures of TbDyFe alloys or other cubic magnetic materials.

      • KCI등재

        Identification of novel alleles induced by EMS-mutagenesis in key genes of kernel hardness and starch biosynthesis in wheat by TILLING

        Wenjie Li,Huijun Guo,Yongbin Wang,Yongdun Xie,Linshu Zhao,Jiayu Gu,Shirong Zhao,Baocun Zhao,Guangjin Wang,Luxiang Liu 한국유전학회 2017 Genes & Genomics Vol.39 No.4

        To identify novel allelic variations in key genes of wheat quality, the present study used the targeting induced local lesions in genomes platform to detect point mutations in target genes. The wheat variety Longfumai 17 was treated by the mutagen ethyl methanesulfonate to produce a bulk M2 generation, and the population included 1122 plants. A total length of 3906.80 kb nucleotides was analyzed, and the average mutation density was 1/244.17 kb. The identified mutations included G>A substitutions (43.75%), C>T substitutions (31.25%), A insertions (12.50%), T insertions (6.25%), and deletions (6.25%). These point mutations led to changes in amino acids and thus the encoded protein sequences, ultimately producing 18.75% of missense mutations, 12.50% of frame shift mutations, 6.25% of nonsense mutations, 25.00% of silent mutations and 37.50% of non-coding region mutations. In the kernel hardness gene Pinb and 3 starch synthesis genes waxy, Agp2 and SSIIa-A, we detected 16 different point mutations in 25 mutant lines. The Pinb gene harbored two missense mutations and a nonsense mutation; the C>T missense mutation resulted in a novel allele, this novel allele and the nonsense mutation alerted protein 3D structure; the waxy gene presented missense and frame shift mutations; the Agp2 gene carried a missense mutation; the SSIIa-A incurred a missense mutation and a frame shift mutation that resulted in premature protein termination. All the frame shift mutations, nonsense mutations and the Pinb novel allele resulted in allelic variation of their corresponding genes, which in turn affected their gene functions. The identified mutant lines can be used as intermediate materials in wheat quality improvement schemes.

      • KCI등재

        Facile preparation and characterization of hollow poly(St-co-MMA-co-BA-co-MAA) core–double shell latex nanoparticles for electrophoretic displays

        Tingfeng Tan,Shirong Wang,Xianggao Li,Caiyan Wang,Yong An 한국물리학회 2009 Current Applied Physics Vol.9 No.5

        Novel core–double shell particles with poly(methyl methacrylate-co-butyl acrylate) (PMMA-co-BA) as the cores, poly(methyl methacrylate-co-butyl acrylate-co-methacrylic acid) (PMMA-co-BA-co-MAA) as the inner shells, poly(styrene-co-methyl methacrylate) (PS-co-MMA) as the outer shells were prepared by soap-free emulsion polymerization. The acid–alkali osmotic swelling processes were made before the outer shells wrapped for bigger aperture. The optimal experiment conditions were summarized. The morphology and size of the hollow latex particles were observed by transmission electron microscopy. The results showed that the uniform sizes of the hollow latex particles were about 230 nm. The electrophoretic mobility of them in tetrachloroethylene was 0.91 × 10-10 m2 V-1 s-1, and the Zeta-potential was 5.87 mV. The results showed that the hollow polymer particles can used as background particles. Novel core–double shell particles with poly(methyl methacrylate-co-butyl acrylate) (PMMA-co-BA) as the cores, poly(methyl methacrylate-co-butyl acrylate-co-methacrylic acid) (PMMA-co-BA-co-MAA) as the inner shells, poly(styrene-co-methyl methacrylate) (PS-co-MMA) as the outer shells were prepared by soap-free emulsion polymerization. The acid–alkali osmotic swelling processes were made before the outer shells wrapped for bigger aperture. The optimal experiment conditions were summarized. The morphology and size of the hollow latex particles were observed by transmission electron microscopy. The results showed that the uniform sizes of the hollow latex particles were about 230 nm. The electrophoretic mobility of them in tetrachloroethylene was 0.91 × 10-10 m2 V-1 s-1, and the Zeta-potential was 5.87 mV. The results showed that the hollow polymer particles can used as background particles.

      • A High-Robust Grid-Side Current Feedback Active Damping Design Method for Gridconnected Inverter

        Ke Xu,Li Peng,Weibiao Wu,Bowei Lin,Shirong Yang 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5

        Grid-connected inverter with LCL filter is widely employed in renewable energy generation system due to high harmonic suppression ability. However, the resonance issue caused by LCL filter is brought easily. To solve this issue, grid-side current feedback active damping control strategy based on proportional derivative algorithm is employed for cost consideration. Taking digital control delay time into consideration, the active damping method is analyzed in discrete domain. A parameter design method based on dominant pole optimization is proposed, which can not only enhance damping ability but also achieve high robust performance. The validity of the proposed method is finally confirmed by the experimental results.

      • KCI등재

        Laccase-Mediated In Situ Oxidation of Dopamine for Dyeing of Human Hair

        Weini Jia,Shirong Li,Zhengxin Luo,Hainan Yu,Wenjun Zhu,Wenjun Zhu,Qing-hui Mao,Xiaoli Zhang,Zhi-jie Liang,Aixue Dong 한국섬유공학회 2021 Fibers and polymers Vol.22 No.1

        Due to the damage of scalp because of chemical dyes, increasing attention had been paid to the ecological dyeingof human hair. To address the need for ecological restoration and dyeing of human hair, herein, a novel approach has beenproposed via laccase-mediated in situ oxidation of dihydroxy phenylalanine (dopamine). As laccase can catalyze dopamineoxidation and then polymerization to produce strongly colored polymers, different biological coloration processes of humanhair based on this process were investigated: (i) Human hair was treated with laccase alone; (ii) The absorption of human hairwith dopamine, followed by further catalysis of air; (iii) The absorption of human hair with dopamine, followed by furthercatalysis of laccase; (iv) Simultaneous laccase-mediated polymerization and dyeing of human hair; and (v) Polymerization ofDopamine with laccase and then dyeing human hair. The surface properties of dyed hair were evaluated in terms of ultraviolet(UV)-protection, color fastness, Fourier transforming infrared spectrum (FT-IR), optical microscope, and scanning electronmicroscopy (SEM). The results show that best hair dyeing effect was achieved via simultaneous laccase-mediatedpolymerization and dyeing. The dyed hair obtained K/S values of 28, light fastness of level 5, and UPF of 100+. The opticalmicroscope and SEM observations show that the surface of colored hair fibers was covered with thin burrs, which can beattributed to polydopamine attached with physical adhesive force and covalent reaction. FT-IR analysis shows that functionalgroups were present on the surface of the dyed hair which can be considered as introduction of hydroxyl radical groups. Thisbiological stain technique may be widely used in dyeing of hair with black color.

      • SCIESCOPUSKCI등재

        Hepatitis B Virus DNA Polymerase Displays an Anti-Apoptotic Effect by Interacting with Elongation Factor-1 Alpha-2 in Hepatoma Cells

        ( Xianli Niu ),( Shirong Nong ),( Junyuan Gong ),( Xin Zhang ),( Hui Tang ),( Tianhong Zhou ),( Wei Li ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 Journal of microbiology and biotechnology Vol.31 No.1

        Hepatitis B virus (HBV) genome P-encoded protein HBV DNA polymerase (Pol) has long been known as a reverse transcriptase during HBV replication. In this study, we investigated the impact of HBV Pol on host cellular processes, mainly apoptosis, and the underlying mechanisms. We showed a marked reduction in apoptotic rates in the HBV Pol-expressed HepG2 cells compared to controls. Moreover, a series of assays, i.e., yeast two-hybrid, GST pull-down, co-immunoprecipitation, and confocal laser scanning microscopy, identified the host factor eEF1A2 to be associated with HBV Pol. Furthermore, knockdown of eEF1A2 gene by siRNA abrogated the HBV Pol-mediated anti-apoptotic effect with apoptosis induced by endoplasmatic reticulum (ER) stress-inducer thapsigargin (TG), thus suggesting that the host factor eEF1A2 is essential for HBV Pol’s anti-apoptosis properties. Our findings have revealed a novel role for HBV Pol in its modulation of apoptosis through integrating with eEF1A2.

      • Diurnal variations and gap effects of soil CO<sub>2</sub>, N<sub>2</sub>O and CH<sub>4</sub> fluxes in a typical tropical montane rainforest in Hainan Island, China

        Yang, Huai,Liu, Shirong,Li, Yide,Xu, Han Springer Japan 2018 Ecological research Vol.33 No.2

        <P><B>Abstract</B></P><P>Accurate estimations of soil greenhouse gas (GHG) fluxes in tropical montane rainforests are critical for assessing the role of tropical forests in influencing global climate change. This research aimed to determine the diurnal variation in soil GHG fluxes and understand the effects of forest canopy gaps on GHG fluxes, and their major controlling factors. The diurnal fluxes of soil carbon dioxide (CO<SUB>2</SUB>), nitrous oxide (N<SUB>2</SUB>O) and methane (CH<SUB>4</SUB>) inside and outside three forest canopy gaps in a tropical montane rainforest were measured with a closed static chamber system in June 2015. The main results are as follows. (1) There was an obvious single‐peak daily variation of soil GHG fluxes. (2) The averaged soil CO<SUB>2</SUB>, N<SUB>2</SUB>O and CH<SUB>4</SUB> fluxes of the whole day were closest to the daily average emission fluxes at 9:00 and 12:00 for CO<SUB>2</SUB>, 6:00 and 9:00 for N<SUB>2</SUB>O, and 9:00 and 12:00 for CH<SUB>4</SUB>, respectively. (3) Soil CO<SUB>2</SUB> and N<SUB>2</SUB>O emissions (positive values) and CH<SUB>4</SUB> uptake (negative values) were higher inside gaps than outside. (4) There were stronger exponential relationships between soil CO<SUB>2</SUB> and N<SUB>2</SUB>O emissions and temperature inside gaps than outside, and there was a stronger quadratic relationship between CH<SUB>4</SUB> uptake and temperature outside gaps than inside. However, significant relationships between soil CO<SUB>2</SUB> (or CH<SUB>4</SUB>) and soil moisture only occurred inside gaps (<I>P</I> < 0.01). There were clear diurnal variations and significant effects of gaps on soil CO<SUB>2</SUB>, N<SUB>2</SUB>O and CH<SUB>4</SUB> fluxes. Our study indicated that understanding the different diurnal variations of soil CO<SUB>2</SUB>, N<SUB>2</SUB>O and CH<SUB>4</SUB> fluxes inside and outside canopy gaps could improve the accurate evaluation of soil GHG fluxes in tropical montane rainforests under a changing climate.</P>

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