Biomonitoring of Indoor Particle Contamination by Detection of Bioluminescence Reduction of Marine Bacterium Vibrio fischeri

Shiro Ikeda,Masafumi Oikawa,Yoshika Sekine 제어로봇시스템학회 2009 제어로봇시스템학회 국제학술대회 논문집 Vol.2009 No.8

Bioassay, which detects biohazardous property of environmental contaminants, is still being an unexploredfield of air quality monitoring. This study aims to develop a simple testing system of toxicity of the indoor particulate matter by detecting bioluminescence reduction of marine bacterium Vibrio fischeri. Suspended particulate matter in indoor and outdoor air was collected on a quartz fiber filter at a flow rate of 23.5 L/min. by a low volume air sampler for 7-day duration, at the terrace and living room of an apartment house in Kanagawa, Japan. Collected materials wereextracted with sterilized distilled water by mild shaking. After filtration with 0.45 ??m pore size filter, the extracts were subsequently mixed with a bacterium solution in a well of 24-well plate and time courses of bioluminescence intensity were measured by a luminometer. As a result, the water extracts of indoor and outdoor aerosol samples significantly reduced the bioluminescence of Vibrio fischeri and the inhibition per sampling volume increased with increasing TSPconcentrations. The inhibition per unit mass of the indoor aerosols became greater than that of outdoor aerosols, whenincreasing in air change rate. The inhibition tended to correlate with nitrate and sulfate concentrations in the extracts. This study showed possible application of bioassay to the monitoring of indoor suspended particle contamination.

Balloon-occluded retrograde transvenous obliteration for six consecutive patients with duodenal varices

Shiro Miyayama,Masashi Yamashiro,Rie Ikeda,Junichi Matsumoto,Nobuhiko Ogawa,Kazuo Notsumata 소화기인터벤션의학회 2022 Gastrointestinal Intervention Vol.11 No.1

Background: Bleeding from duodenal varices is a rare but life-threatening complication of portal hypertension. The treatment of duodenal varices remains difficult and a definitive treatment strategy has not been established. The aim of this study was to report the technical aspects and outcomes of balloon-occluded retrograde transvenous obliteration (BRTO) using 5% ethanolamine oleate with iopamidol (EOI) for duodenal varices. Methods: Six consecutive patients with duodenal varices treated using BRTO were eligible. Endoscopic treatment was performed first in three patients with active bleeding. After coil embolization of collateral veins, stepwise EOI infusion was performed at intervals of 10–30 minutes under balloon occlusion until the main efferent vein, varices, and the main afferent vein were filled with EOI and clots. The techniques and outcomes of BRTO were retrospectively evaluated. Results: The main efferent vein of duodenal varices was the right (n = 4) or left (n = 2) gonadal vein. In three patients with ruptured varices, BRTO was performed after achieving hemostasis by endoscopic treatment. In five patients, 1–4 (mean, 2.4 ± 1.1) collateral veins were embolized with coils before EOI infusion. Furthermore, 11–21 mL (mean, 15.3 ± 4.2 mL) of EOI was infused by 3–5 (mean, 3.5 ± 1.0) stepwise infusions via the efferent vein under balloon occlusion. The duration of EOI infusion under balloon occlusion ranged from 82 to 118 minutes (mean, 87.8 ± 13.6 minutes). The varices were thrombosed in all but one patient. In the remaining patient, the varices were thrombosed by additional BRTO under overnight balloon occlusion performed 19 days later. The only complications were a transient fever and hematuria. All duodenal varices disappeared during a followup of 4–32 months (mean, 16.2 ± 11.1 months) after BRTO. Conclusion: BRTO using EOI is an effective treatment for duodenal varices.

Balloon-occluded retrograde transvenous obliteration for six consecutive patients with duodenal varices

Shiro Miyayama,Masashi Yamashiro,Rie Ikeda,Junichi Matsumoto,Nobuhiko Ogawa,Kazuo Notsumata 소화기인터벤션의학회 2022 International journal of gastrointestinal interven Vol.11 No.1

Background: Bleeding from duodenal varices is a rare but life-threatening complication of portal hypertension. The treatment of duodenal varices remains difficult and a definitive treatment strategy has not been established. The aim of this study was to report the technical aspects and outcomes of balloon-occluded retrograde transvenous obliteration (BRTO) using 5% ethanolamine oleate with iopamidol (EOI) for duodenal varices. Methods: Six consecutive patients with duodenal varices treated using BRTO were eligible. Endoscopic treatment was performed first in three patients with active bleeding. After coil embolization of collateral veins, stepwise EOI infusion was performed at intervals of 10–30 minutes under balloon occlusion until the main efferent vein, varices, and the main afferent vein were filled with EOI and clots. The techniques and outcomes of BRTO were retrospectively evaluated. Results: The main efferent vein of duodenal varices was the right (n = 4) or left (n = 2) gonadal vein. In three patients with ruptured varices, BRTO was performed after achieving hemostasis by endoscopic treatment. In five patients, 1–4 (mean, 2.4 ± 1.1) collateral veins were embolized with coils before EOI infusion. Furthermore, 11–21 mL (mean, 15.3 ± 4.2 mL) of EOI was infused by 3–5 (mean, 3.5 ± 1.0) stepwise infusions via the efferent vein under balloon occlusion. The duration of EOI infusion under balloon occlusion ranged from 82 to 118 minutes (mean, 87.8 ± 13.6 minutes). The varices were thrombosed in all but one patient. In the remaining patient, the varices were thrombosed by additional BRTO under overnight balloon occlusion performed 19 days later. The only complications were a transient fever and hematuria. All duodenal varices disappeared during a followup of 4–32 months (mean, 16.2 ± 11.1 months) after BRTO. Conclusion: BRTO using EOI is an effective treatment for duodenal varices.

Outcomes of conventional transarterial chemoembolization using guidance software for hepatocellular carcinoma in the caudate lobe of the liver

Miyayama Shiro,Yamashiro Masashi,Ikeda Rie,Yokka Akira,Komiya Hideaki,Sakuragawa Naoko 소화기인터벤션의학회 2023 International journal of gastrointestinal interven Vol.12 No.2

Background: To evaluate the outcomes of conventional transarterial chemoembolization (TACE) using guidance software for hepatocellular carci-noma (HCC) in the caudate lobe.Methods: Seventy-two patients with 79 treatment-naïve caudate lobe HCCs were eligible. TACE was performed through feeders not only identified by automated tumor-feeder detection (AFD) functionality but also detected manually. Technical success of TACE was classified into three grades accord-ing to 1-week computed tomography findings: entire tumor embolized with a safety margin (5 mm for tumors < 25 mm and 10 mm for tumors ≥ 25 mm) (grade A); tumor embolized without a margin in parts (grade B); and entire tumor not embolized (grade C). Tumor response was evaluated using the modified Response Evaluation Criteria in Solid Tumors. Tumor-feeder detectability by AFD, technical success of TACE, complete response (CR) at 2–4 months, durable CR, and local tumor progression (LTP) rates calculated by the Kaplan-Meier method were compared in each tumor among three subsegments: the Spiegel lobe (SP); paracaval portion (PC); and caudate process (CP). LTP rates between the grade A and B tumors were also com-pared.Results: The mean tumor diameter was 18.6 ± 9.9 mm (range, 6–53 mm), and 111 of 129 (86.0%) tumor feeders were detected by AFD. The rates of feeder detectability by AFD, grade A technical success of TACE, CR at 2–4 months, and durable CR in 30 SP, 38 PC, and 11 CP tumors were 71.4%, 93.3%, 93.1%, and 79.3%; 94.8%, 65.8%, 59.4%, and 34.4%; and 76.5%, 63.6%, 80.0%, and 30.0%, respectively. LTP rates of SP tumors were sig-nificantly lower than those of PC tumors (P = 0.0044), and the grade B tumors progressed more frequently (P = 0.0012).Conclusion: AFD could detect 86.0% of tumor feeders in the caudate lobe; however, the feeder detectability, technical success of TACE, and outcomes differed among the three subsegments.

The Improvement of the Permeability of Wood by Underwater Shock Wave

Itoh, Shigeru,Nagano, Shiro,Fujita, Masahiro,Takano, Tsutomu,Honda, Ryouzou,Ikeda, Motoyosi 대한금속재료학회(대한금속학회) 1998 METALS AND MATERIALS International Vol.4 No.4

Sugi (Cryptomeria japonica D.Don) wood generally has the so-called black heartwood, which contains the extremely high moisture content. Although subjected to the drying process, it is difficult to be dried up completely so that Sugi wood does not have the sufficient strength for use. The reason is that during drying process, the bordered pit membrane on traicheids closes faster near the heartwood in Sugi wand so that the internal moisture is not easily came out to the outside. At the mean time, it also makes very difficult ta saturate the rotten-proof chemicals into the dried Sugi wood to leave it become an additionally valuable wand. In this paper, we will develop an underwater shock wave technique to treat the pre-dried wood for the; improvement of the drying property as well as the permeability rind strength of Sugi wood by selectively fracturing the bordered pit membrane. The result indicates a very remarkable improvement c7n Che drying time. The. mechanism of the fracture of the bordered pit by underwater shock wave will be further studied ire the future.