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RISS 인기검색어
- 검색키워드
- 저자 : Shiqing Peng (검색결과 3 건)
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Zhang, Shiqing,Sun, Peng,Xiao, Xinru,Hu, Yujie,Qian, Yan,Zhang, Qian The Korean Society of Pharmacology 2022 The Korean Journal of Physiology & Pharmacology Vol.26 No.4
Epithelial-mesenchymal transition (EMT) is known to be involved in airway remodeling and fibrosis of bronchial asthma. However, the molecular mechanisms leading to EMT have yet to be fully clarified. The current study was designed to reveal the potential mechanism of microRNA-21 (miR-21) and poly (ADP-ribose) polymerase-1 (PARP-1) affecting EMT through the PI3K/AKT signaling pathway. Human bronchial epithelial cells (16HBE cells) were transfected with miR-21 mimics/inhibitors and PARP-1 plasmid/small interfering RNA (siRNA). A dual luciferase reporter assay and biotin-labeled RNA pull-down experiments were conducted to verify the targeting relationship between miR-21 mimics and PARP-1. The migration ability of 16HBE cells was evaluated by Transwell assay. Quantitative real-time polymerase chain reaction and Western blotting experiments were applied to determine the expression of Snail, ZEB1, E-cadherin, N-cadherin, Vimentin, and PARP-1. The effects of the PI3K inhibitor LY294002 on the migration of 16HBE cells and EMT were investigated. Overexpression of miR-21 mimics induced migration and EMT of 16HBE cells, which was significantly inhibited by overexpression of PARP-1. Our findings showed that PARP-1 was a direct target of miR-21, and that miR-21 targeted PARP-1 to promote migration and EMT of 16HBE cells through the PI3K/AKT signaling pathway. Using LY294002 to block PI3K/AKT signaling pathway resulted in a significant reduction in the migration and EMT of 16HBE cells. These results suggest that miR-21 promotes EMT and migration of HBE cells by targeting PARP-1. Additionally, the PI3K/AKT signaling pathway might be involved in this mechanism, which could indicate its usefulness as a therapeutic target for asthma.
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Zhibo Hao,Tian Tian,Shiqing Peng,Changchun Ge,Xinggang Li,Chonglin Jia,Chuan Guo,Qiang Zhu 대한금속·재료학회 2020 METALS AND MATERIALS International Vol.26 No.8
A self-designed nickel-based superalloy, designated as FGH4096M, was prepared by selective laser melting (SLM). Differentheat treatments were performed to improve the mechanical properties of the SLM alloy through optimizing the microstructuresand the features of γ′ precipitates. Compared with the as-deposited alloy, the columnar grains with dendritic structuresand equiaxed structures were retained in the alloy after direct aging, but a large amount of tertiary γ′ phase precipitated,especially around the sub-grain boundaries, resulting in the highest tensile strength but the lowest elongation. During solidsolution and aging treatment (SSA), the recovery and recrystallization occurring in the alloy facilitated the grains to beequiaxed with the increase of solution temperature. For lower solution temperature (below 1100 ℃), the secondary γ′ precipitatesdecreased with the increase of solution temperature, while the tertiary γ′ precipitates from the subsequent agingprocess gradually increased; for higher solution temperature over 1100 ℃, exceeding the complete dissolution temperatureof the γ′ phase, only tertiary γ′ precipitates from the subsequent aging process were uniformly distributed in the alloy. Afterdouble solid solution (1170 ℃ + 1050 ℃) + aging heat treatment (DSSA), there were three sizes of γ′ precipitates in thealloy. In general, the SLM + SSA (1130 ℃) alloy obtained the best comprehensive properties, which could be related tothe homogenized microstructures and the uniform and dense distribution of single sized tertiary γ′ precipitates in the alloy.
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Li Xuewen,Liu Zihao,Xu Shendan,Ma Xinying,Zhao Zhezhe,Hu Han,Deng Jiayin,Peng Cheng,Wang Yonglan,Ma Shiqing 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00
Exosomes derived from bone marrow mesenchymal stem cells (BMSC-exos) have been shown triggering osteogenic differentiation and mineralization of MSCs, but exosomes administered via bolus injections are rapidly sequestered and cleared. Therefore, we considered the implant as a new organ of patient’s body and expected to find a method to treat implant with BMSC-exos in vivo directly.A fusion peptide (PEP), as a drug delivery system (DDS) which contained a titanium-binding peptide (TBP) possessing the ability to selectively bind to the titanium surface and another peptide CP05 being able to capture exosomes expertly, is constructed to modify the titanium surface.Both in vitro and in vivo experiments prove PEP retains the ability to bind titanium and exosome simultaneously, and the DDS gain the ability to target exosomes to titanium implants surface following enhancing osseointegration post-implantation. Moreover, the DDS constructed by exosomes of diverse origins shows the similar combination rate and efficiency of therapy.This drug delivery system demonstrates the concept that EXO-PEP system can offer an accurate and efficient therapy for treating implants with long-term effect.
ScienceON
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