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Shin-ichiro Ogura,Shigefumi Tanaka,Shin Matsumoto,Kunihiro Abe,Toshio Shimizu,Yoshihiro Takahashi,Hiroshi Yoshida 한국초지조사료학회 2009 한국초지조사료학회 학술대회논문집 Vol.2009 No.08
Our previous study conducted in a wasted mulberry plantation showed significant decline of leaf productivity of mulberry (Morns sp.) by cattle grazing in three years (Ogura et al., 2009). This means t㏊t improvement of understory vegetation is essential to use such wasted areas as grazing lands. In this study, the mixture of seeds (Dactylis glomerata, Lolium perenne, Poa pratensis and Trifolium repens, 15:15:5:2) was seeded on 18 Sep. 2007, in nine experimental plots (6 m × 6 m each) with different seeding rates (L: 37 ㎏/㏊, M: 56㎏/㏊ and H: 74㎏/㏊, three replications) in the wasted mulberry plantation described in the previous paper (paddock A) (Ogura et al., 2009). Two cows were grazed in the paddock for 42 and 54 days in 2007 and 2008, respectively. No fertilizer nor herbicides were applied in the paddock. The coverage of the sown species and weeds, and the proportion of bare area were recorded on 19 Sep. 2007, 16 Nov. 2007, 21 May 2008 and 21 May 2009. T. repens rapidly covered the understory; the coverage ranged 49.4-71.7% in 2008-2009 (Fig. 1). D. glomerata and L. perenne also increased to 10.6-18.3% and 20.6-33.9% in 2009, respectively, regardless to the seeding rates. In contrast, the proportion of bare area rapidly decreased. The quick establishment of the sown plants probably due t㏊t defoliation and trampling of cows suppressed the growth of native plants. In conclusion, the seeding rate of 3.7 ㎏/㏊ is sufficient to establish herbage plants in wasted mulberry plantations under cattle grazing.
Productivity and Quality of Mulberry Leaves under Cattle Grazing in a Wasted Mulberry Plantation
Shin-ichiro Ogura,Shusuke Sato,Shigefumi Tanaka,Shin Matsumoto,Kunihiro Abe,Toshio Shimizu,Yoshihiro Takahashi,Hiroshi Yoshida 한국초지조사료학회 2009 한국초지조사료학회 학술대회논문집 Vol.2009 No.08
Mulberry (Morus sp.) plantations have been deteriorating in Japan due to decline of silk industry. In mountainous areas, large part of the mulberry plantations has been wasted. Cattle grazing in the wasted mulberry plantations is a practical system to reuse the wasted areas for food production, because mulberry leaves are highly nutritive (Ezenwa and Kitahara, 2001), productive (Kitahara et al., 2002) and palatable to cattle (Ogura et al., 2008). In this study, the productivity and quality of mulberry leaves were investigated for 3 years in a wasted mulberry plantation grazed with beef cows, in Minamisanriku town, Miyagi, Japan. Cattle grazing started in September 2005 (paddock A, 44 a) and June 2006 (paddock B, 96 a). For each paddock, two pregnant cows were grazed from June to August-October in 2006-2008. In the paddocks A and B, four mulberry trees were chosen and the leaf mass and chemical composition were measured in mid-June and late August (pre-grazing leaf mass) during 2006-2008. A non-grazed paddock was also measured in 2007-2008. Leaf productivity of mulberry trees significantly decreased with cattle grazing; pre-grazing leaf mass changed from 246.8 g to 10.4 g DM/tree and 286.2 g to 17.6 g DM/tree in the paddock A and B, respectively (Fig 1). In contrast, 149.2-365.1 g DM/tree of leaves was produced in the non-grazed paddock. Chemical composition was stable during the three years in all paddocks. It is concluded that cattle grazing gives severe damage to leaf production of mulberry trees in three years.
Matsumoto, Shuichi,Hosozawa, Osamu,Narihara, Hiroyuki,Komuro, Tsutomu,Kawamoto, Shin-ichiro Council on Tall Building and Urban Habitat Korea 2014 International journal of high-rise buildings Vol.3 No.1
In recent years, the performance requested for which an ultra-high rise buildings is diversified. Large spans are designed in order to gain wide workspace. Column positions are shifted in middle stories to provide space different from neighboring floors. Moreover, in the bottom layers of the building, it is becoming more important to expand freedom to plan flexibility such as creating publically opened wide atria that gives attractive free space. Earthquake-proof criteria is also changing not only human life protection deign but also a design that allows functional continuity. In order to achieve thee needs, as one of technology, we have developed ultra-high strength concrete filled tubular (CFT) columns of the box section that combine ultra-high strength concrete with specified strength of $150N/mm^2$ and ultra-high strength steel material with tensile strength of $780N/mm^2$. In this paper, the outline of development of an ultra-high strength CFT column is reported. Also, the structural design of the ultra-high-rise building using the CFT columns is reported.
뇌염모델에서 Protein Kinase C의 발현에 관한 연구
Shin, Tae-Kyun,Kim, Hyung-Min,Tanuma, Naoyuki,Matsumoto, Yoh 한국수의병리학회 1997 한국수의병리학회지 Vol.1 No.1
Protein kinase C an enzyme of signal transduction has been known to regulate cell proliferation activation as well as apoptosis in some cancer cell lines. To explore the role of PKC in the course of cell mediated autoimmune disease such as experimental autoimmune encephalomyelitis (EAE) EAE was induced in Lewis rats(6-8 weeks old) with immunization of myelin basic protein supplemented with complete Freund's adjuvants and affected spinal cords were sampled at days 13 postimmunization(PI) as peak stage of EAE and at days 21 PI as recovery stage. The spinal cords with EAE were subjected to Northern blot analysis and insitu hybridization of PKC delta which is one of prominant isotypes of PKC in the haematopoietic cells. Northern blot analysis showed that levels of PKS delta mRNA in the spinal cords of rats withEAE was significantly increased at days 13 PI in which inflammatory cells including T cells and macrophages in the EAE lesions appeared. however the stage. By in situ hybridization signals of PKC delta in EAE lesions was intensely expressed on the delta is also expressed on some brain cells in normal rat central nervous system This finding suggests that PKC plays an important role on either activation of inflammatory cells including encephalitogenic T cells and macrophages or apoptotic elimination of some inflammatory cells depending on the stge of EAE.
Mechanism of experimental autoimmune encephalomyelitis in Lewis rats
Taekyun Shin,Meejung Ahn,Yoh Matsumoto 대한해부학회 2012 Anatomy & Cell Biology Vol.45 No.3
Experimental autoimmune encephalomyelitis (EAE) in Lewis rats is an acute monophasic paralytic central nervous system disease, in which most rats spontaneously recover from paralysis. EAE in Lewis rats is induced by encephalitogenic antigens, including myelin basic protein. EAE is mediated by CD4⁺ Th1 cells, which secrete pro-inflammatory mediators, and spontaneous recovery is mediated by regulatory T cells. Recently, it was established that classically activated macrophages (M1 phenotype) play an important role in the initiation of EAE, while alternatively activated macrophages (M2 phenotype) contribute to spontaneous recovery from rat EAE. This review will summarize the neuroimmunological aspects of active monophasic EAE, which manifests as neuroinflammation followed by neuroimmunomodulation and/or neuroprotection, with a focus on the role of alternatively activated macrophages.
Oh, Hanseul,Ahn, Meejung,Matsumoto, Yoh,Shin, Taekyun The Korean Society of Veterinary Science 2013 大韓獸醫學會誌 Vol.53 No.4
To better understand the role of macrophages in early stages of experimental autoimmune myocarditis (EAM), we compared the expression of inducible nitric oxide synthase (iNOS) and arginase-1, markers for classically activated M1 and alternatively activated M2 macrophages, respectively, in the hearts of EAM-affected and control rats. Immunohistochemical evidence revealed that both iNOS-positive and arginase 1-positive macrophages were found in EAM lesions, while some cells were co-localized with both markers. This finding suggests that the increased level of arginase-1, which is partly from M2 macrophages, contributes to the modulation of EAM, possibly through the reduction of nitric oxide in the lesion.