Study on Intracellular Zinc Uptake According to Zinc-ligand

Shim, Boo-Im,Kim, Ki-Nam,Kim, Yu-Ri,Lee, Seung-Ho,Lee, Seung-Min,Park, Myung-Gyu,Kim, Meyoung-Kon The Korean Society of Toxicogenomics and Toxicopro 2007 Molecular & cellular toxicology Vol.3 No.4

Zinc plays indispensable roles in metabolism, including cell growth, apoptosis, proliferation and differentiation. Kidneys are target organs for various regulators of mineral metabolism, and play a key role in zinc balance. To investigate the zinc uptake efficiency, we examined the zinc uptake and accumulation level in vivo and in vitro study. Plasma zinc concentration was peaked out at 1 hr after oral zinc administration. The renal zinc level was peaked out at 12 hr after oral zinc administration, and it was the highest in 40 mg/kg Zn-Asp administrated group in comparison with other groups. In addition, the m-RNA expression level of zinc transporter-1 (ZnT-1), zinc transporter-2 (ZnT-2) and high-affinity L-aspartate transporter (EAAT-3) in Zn-Asp administered group were increased compared with control groups and $ZnSO_4$ group. In order to investigate the intracellular zinc uptake mechanism, we performed the in vitro study by using human embryonic kidney cell line, HEK 293. Intracellular zinc level was peaked out at 3 hr after zinc treatment. In the same way, the mRNA expression level of ZnT-1 and EAAT-3 were increased compared with control group. This study showed that Zn-Asp is effective the zinc uptake into the kidney by increasing the zinc transporter expression.

The Efficiency of Zinc-Aspartate Complex on Zinc Uptake in Plasma and Different Organs in Normal SD Rats

Kim, Yu-Ri,Kim, Ki-Nam,Shim, Boo-Im,Lee, Seung-Min,Kim, In-Kyoung,Sohn, Sung-Hwa,Park, Myung-Gyu,Park, Hong-Suk,Kim, Meyoung-Kon The Korean Society of Toxicogenomics and Toxicopro 2007 Molecular & cellular toxicology Vol.3 No.2

Zinc is essential metal and plays a role in a wide variety of physiological and biochemical processes. Prostate gland contains high level of zinc, generally 3-10 folds higher than other organs. Prostatic zinc uptake is resulted from the existence of zinc transporter (ZnT) protein families in membrane. In this study, we investigated the difference of zinc uptake efficiency of zinc-aspartate complex (Zn-Asp) into various organs compared with $ZnSO_4$. We observed that Plasma zinc concentration in both $ZnSO_4$ and Zn-Asp administrated group was increased progressively following administration, and reached a peak level at 2 hr. The increasing pattern of zinc concentration was similar to each groups, however the zinc concentration of Zn-Asp administrated group was higher than that of $ZnSO_4$ administrated group. We found that prostatic zinc level of Zn-Asp administrated group was higher than $ZnSO_4$ administrated group, and was increased approximately $\sim$2.7 fold and $\sim$4.2 fold at 4 and 8 hr after administration. From these observations, we suggest than Zn-Asp has high uptake efficiency of zinc into the prostate gland. Therefore, Zn-Asp is potentially useful treatment of many prostatic diseases.

이러닝 1 : 영역 관련 단어를 이용한 웹문서 분류 시스템 설계 및 구현

강원석 ( Won Seog Kang ),임대현 ( Dae Hyun Im ),심은희 ( Eun Hey Shim ),문승은 ( Seung Eun Moon ),김상희 ( Sang Hey Kim ),정부천 ( Boo Cheon Jung ) 한국컴퓨터교육학회 2009 한국컴퓨터교육학회 학술발표대회논문집 Vol.13 No.1

정보사회에서 필요로 하는 정보를 신속히 찾기 위하여 정보를 분류할 필요가 있다. 본 연구는 웹에 있는 웹 문서를 자동으로 분류하는 시스템을 제안한다. 본 시스템은 문서 분류의 효과를 향상하기 위하여 영역 관련단어를 조사한 후 이를 이용하는 방법을 사용한다. 시스템을 비학습 자료에 대하여 실험한 결과 영역관련 단어를 이용한 방법이 더 나음을 보였다. 본 시스템에 사용한 영역 관련 단어는 정보검색 분야에 이용가치가 높다.

건강자원자의 직장항문 생리검사에서 골반저 조율장애 진단의 위양성 결과에 대한 정신사회적 인자의 영향

송해정 ( Hae Jung Song ),이준성 ( Joon Seong Lee ),임희혁 ( Hee Hyuk Im ),홍수진 ( Su Jin Hong ),정인섭 ( In Seop Jeong ),조주영 ( Joo Young Cho ),김진오 ( Jin Oh Kim ),심찬섭 ( Chan Sup Shim ),김부성 ( Boo Sung Kim ) 대한소화기기능성질환·운동학회 2006 Journal of Neurogastroenterology and Motility (JNM Vol.12 No.1

목적: 정상인에서도 직장항문 생리검사 시 간혹 골반저 조율장애로 진단되는 위양성의 결과가 나온다. 본 연구의 목적은 건강자원자에서 직장항문 생리검사의 결과에 대한 정신사회경제적인 면이 미치는 영향에 대하여 알아보고자 하였다. 대상 및 방법: 40명의 건강자원자를 대상으로 전향적인 연구를 시행하였다. 모든 자원자들에게 정신건강상태 측정 및 개인력에 대하여 조사하고, 항문직장 내압검사, 풍선배출검사, 근전도 검사, 배변조영술, 대장통과시간을 측정하였다. 각 기능검사 결과로 골반저 조율장애를 진단함에 있어 정상군와 비정상군으로 나누어 각각의 정신사회적인 인자들을 비교분석하였다. 결과: 안정시와 압착시의 항문직장각의 차이가 15도 이하인 군과 15도를 초과하는 군을 비교하였을 때, 15도 이하인 군에서 신체화 점수가 더 높게 나타나는 경향을 보였으나 나머지 결과는 정신사회적 인자와는 차이가 없었다(p=0.092). 골반저 조율장애를 보인 군이 정상으로 관찰된 군에 비해 대학에 재학 중이거나 대학을 졸업한 사람들이 통계적으로 유의하게 더 많았다(p<0.05). 또한 골반저 조율장애를 보이는 군에서 정상으로 관찰되는 군에 비해 경제적인 여유가 있는 사람이 더 많았다(p<0.05) 결론: 항문직장 생리검사에서 정신건강적인 요인은 영향을 미치지 않을 것으로 생각하며 사회경제적인 요인은 배변조영술의 결과에 영향을 줄 수 있을 것으로 생각하나 이에 대해서는 더 연구가 필요하다. Background/Aims: False positive results are common in the diagnosis of pelvic floor dyssynergia (PFD) using anorectal physiologic studies (ARPS). The aim of this study was to determine the effects of psychosocial factors on false positive results of ARPS for the diagnosis of PFD in healthy volunteers. Methods: Forty healthy subjects were enrolled. All subjects underwent a complete self-rated inventory assessing psychological distress (SCL-90-R), personal history, anorectal manometry, balloon expulsion testing, electromyography, video-defecography and evaluation of colonic transit time. We compared a variety of psychosocial parameters between subjects with abnormal and normal results of the ARPS. Results: There was a tendency to have a more somatizing personality in subjects with changes in the anorectal angle less than 15 degrees during straining compared to subjects with a change in the anorectal angle more than 15 degrees (p=0.092). However, there were no other differences in psychosocial factors when compared to the results of the anorectal physiologic study. A group with suggestive pelvic floor dyssynergia by video-defecography had more college graduates than the normal group (p<0.05). Also, this group had a greater income than the normal group (p<0.05). Conclusions: Our findings suggest that psychological factors do not affect the results of ARPS. However, socioeconomic factors affect the results of defecography in the diagnosis of PFD in healthy subjects. (Kor J Neurogastroenterol Motil 2006;12:48-54)

장기간 추적 검사가 가능했던 위암환자 1예

신영신,정인식,양진모,김판규,심상인,정규원,김부성,선희식,이교영,채현석,임응훈 대한소화기내시경학회 1991 Clinical Endoscopy Vol.11 No.2

Gastric cancer is the most common malignancy in Korea. Although five years survival rate of gestric cancer has been gradually improved due to advances in the diagnostic technique and therapeutic modalities, the advanced gastric cancer remains a disease with poor pragnosis. At the time of diagnosis, the disease, is usually advanced and longterm survivor is uncommon without a proper surgical treatment or chemotherapy. We experienced a case of advanced gastric cancer who lived for more than 6 years after making a diagnosis, without curative surgical therapy or chemotherapy. The patient at age of 76 year-old, was seen in St. Mary's hospital due to gastroinestinal bleeding in January of l985. A large ulcerating cancer in the diameter of 2.0 cm was found at the posterior wall of body with endoscopy and confirmed the diagnosis of stomach cancer by the endoscopic biopsy. He was recommended the surgical intervention of gastric cancer but he denied the operation due to his age, He had been treated symptomatically with H2 receptor antagonist and antacid with relief of symptoms. Thereafter he had been followed-up clinically at every 2-4 weeks, and the lesion was examined 3-4 times in a year endoscopically, In May l990, an endoscopic esophageal dilation was done due to esophageal stricture with tumor invasion at the lower esophageal sphincter. He was expired in July, 1991 in this hospital. Here we report a case of long term survived advanced gastric cancer who had been followed-up for 6 and half years clinically and endoscopically with review of literatures.

The Anti-Inflammatory Effect of IH-901 in HT-29 Cells

Lee, Seung-Min,Kim, Ki-Nam,Kim, Yu-Ri,Kim, Hye-Won,Shim, Boo-Im,Lee, Seung-Ho,Bae, Hak-Soon,Kim, In-Kyoung,Kim, Meyoung-Kon The Korean Society of Toxicogenomics and Toxicopro 2007 Molecular & cellular toxicology Vol.3 No.4

20-O-($\beta$-D-Glucopyranosyl)-20 (S)-protopanaxadiol (IH-901) is one of the major metabolites of ginsenosides from Panax ginseng, and is suggested that IH-901 has been associated with various pharmacological and physiological activities. In this study, we demonstrate that IH-901 induced anti-inflammation in HT-29 human colon adenocarcinoma cells. Our results showed that IH-901 inhibited cell proliferation of HT-29 in a time- and dose-dependent manner. We also found that IH-901 was significantly decreased expression of iNOS compared with non-treated. We observed effect of IH-901 related with inflammatory genes using by cDNA microarray. We were known that the 34 inflammatory genes such as E2F, CDK6, TNF-$\alpha$, and PKC were down-regulated. Thus, these results suggest that IH-901 may have a potential preventive factor to improving cancer induced by chronic inflammation.

아칼라지아 환자에서 보툴리늄 독소주입 치료 후 미만성 식도경련 , 호두까기 식도로의 이행에 관여하는 인자에 대한 연구

차상우(Sang Woo Cha),이준성(Joon Seong Lee),임희혁(Hee Hyuk Im),황경란(Kyung Ran Hwang),정인섭(In Sup Jung),천갑진(Gab Jin Cheon),김진오(Jin Oh Kim),조주영(Joo Young Cho),이문성(Moon Sung Lee),심찬섭(Chan Sup Shim),김부성(Boo Sung Ki 대한소화기기능성질환·운동학회 2001 Journal of Neurogastroenterology and Motility (JNM Vol.7 No.2

N/A Backgroud/Aims: To evaluate the factors which are related to the transition from achalasia to diffuse esophageal spasm (DES) or nutcracker esophagus (NE) after botulinum toxin inj ection to lower esophageal sphincter (LES). Methods: This study included the 23 patients with achalasia who received an intrasphincteric injection of botulinum toxin. Stational esophageal manometry, 24-hour ambulatory esophageal manometry with Ph monitoring, barium esophagogram and endoscopic ultrasonography were performed before and after treatment. We analyzed the parameters from these studies between the cases that transformed to DES or NE within a week and the cases that do not transit. Result: Five patients (21.7%) transformed to DES (1) or NE (4) within a week. There were significant differences in contraction amplitude of esophageal body (median, 31mmHg vs 23 mmHg, p<0.05) and maximal diameter of esophageal body (median, 2.6 cm vs 4.4 cm, p<0.05) between these five patients and the remaining patients. There were no significant differences in sex, LES pressure and thickness of muscle layer between two groups. Conclusion: Factors involved in transition to NE or DES after botulinum toxin injection to LES of achalasia appears as high amplitude contractions in body of esophagus and less dilation of esophageal body. (Korean Journal of Gastrointestinal Motility 2001;7:188-196)

Application of Differential Expression of Genetic Profiles in Brain Tumors with Variable [$^{18}F$]-fluorodeoxyglucose Uptake

Lee, Seung-Ho,Yun, Mi-Jin,Kim, Ki-Nam,Seo, Sang-Hui,Sohn, Sung-Hwa,Kim, Yu-Ri,Kim, Hye-Won,Kim, In-Kyoung,Shim, Boo-Im,Lee, Seung-Min,Kim, Meyoung-Kon The Korean Society of Toxicogenomics and Toxicopro 2007 Molecular & cellular toxicology Vol.3 No.3

[ $^{18}F$ ]-fluorodeoxyglucose (FDG) uptake on positron emission tomography (PET) scan has been found to reflect tumor aggressiveness and prognosis in various types of cancer. In this study, the gene expression profiles of glial tumors were evaluated to determine whether glial tumors with high $^{18}F$-FDG uptake have more aggressive biological potential than with low uptake. Surgical specimens were obtained from the 12 patients with glial tumors (4 males and 8 females, age range 42-68 years). The tumor samples were divided into two groups based on the $^{18}F$-FDG uptake PET scan findings: high $^{18}F$-FDG uptake (n=4) and low $^{18}F$-FDG uptake (n=8). The pathological tumor grade was closely correlated with the $^{18}F$-FDG uptake pattern: Glial tumors with high $^{18}F$-FDG uptake were pathologically Edmondson-Steiner grade III, while those with low uptake were grade II. The total RNA was extracted from the frozen tissues of all glial tumors (n=12), and adjacent non-cancerous tissue (n=3). The gene expression profiles were evaluated using cDNA microarray. The glial tumors with high $^{18}F$-FDG uptake showed increase expression of 15 genes compared to those with low uptake (P<0.005). Nine genes were down-regulated. Gene expression is closely related to cell survival, cell-to-cell adhesion or cell spreading; therefore, glial tumors with high $^{18}F$-FDG uptake appear to have more aggressive biological properties than those with low uptake.