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Zhi-Hai Ni,Xian-Jun Liang,Jing-Gang Mo,Yi Zhang,Jian-Hua Liang,Yu-Sha Yang,Yong Zhou,Zhao-Hua Li,Jian-Liang Zhang,Yin-Lu Ding,Peng Zhang,Jin-Qing Wang 한국유전학회 2015 Genes & Genomics Vol.37 No.11
We aim to investigate the correlations of hypoxia inducible factor-1a (HIF-1a) C1772T (rs11549465) and G1790A (rs11549467) gene polymorphisms with digestive tract cancers. A sum of 267 digestive tract cancers patients were hospitalized in Taizhou Central Hospital of Zhejiang Province as case group between December 2012 and December 2014. Additionally, 275 healthy people who had a physical examination in our hospital at the same time were selected as control group. Polymerase chain reaction-restriction fragment length polymorphism was utilized for detecting allele and genotype frequency of different locus in case and control group. Meta-analysis was performed using Comprehensive Metaanalysis 2.0 (Biostat Inc., Englewood, New Jersey, USA). Our result showed statistical significance only exists in family history of cancer between case and control group (P\0.05). Both C1772T (rs11549465) and G1790A (rs11549467) polymorphisms showed positive correlations with an increasing risk of digestive tract cancers. The frequencies of TT genotype of C1772T (rs11549465) and GA, AA genotypes of G1790A (rs11549467) polymorphisms in case group were evidently higher compared with the controls (all P\0.05). Besides, the comparison of allele and dominant models of HIF-1a C1772T (rs11549465) and G1790A (rs11549467) between two groups showed a significant difference (all P\0.05). Meta-analysis results further confirmed that the onset risk of digestive tract cancers may be improved under allele and dominant models of HIF-1a C1772T (rs11549465) and G1790A (rs11549467) (all P\0.05). Single nucleotide polymorphisms of HIF-1a C1772T (rs11549465) and G1790A (rs11549467) may play a role in development of digestive tract cancers.
Peng Zhu,An-Qin Duan,Ting-Xian Deng,Xing-Rong Lu,Xiao-Ya Ma,Sha-Sha Liang,Chun-Ying Pang,Xian-Wei Liang 한국생물공학회 2017 Biotechnology and Bioprocess Engineering Vol.22 No.5
In recent years, CRISPR/Cas9 has rapidly become one of the most promising genome editing tools because it is simple and easy to use and cost effective. However, the large size of Cas9 sequences limits its application in clinically promising vectors and it also impacts non-viral transfection. In this study, CRISPR/Cas9 adenovirus vectors that target the buffalo 18s rDNA gene were constructed, transfected into 293 cells for adenovirus packaging, and the adenovirus was used to knockout the 18s rDNA gene in buffalo mammary epithelial cells. The results demonstrated that the CRISPR/Cas9 adenovirus vectors for the buffalo 18s rDNA gene could efficiently target the sites as revealed by the fluorescence reporter system. After amplification, the adenovirus titer of Sn458- 18s1 and Sn458-18s2 reached 1.03 × 109PFU/mL and 1.05 × 109 PFU/mL, respectively. For buffalo mammary epithelial cell infection, the efficiency was 100% when the multiplicity of infection (MOI) 100 PFU/mL. There were 9 mutational clones found in the 20 clones, and the gene mutagenesis rate reached 45%. Of these, 2 clones were 35-bp deleted and 7 clones were 12-bp deleted. These results suggested that the adenovirus system overcame the low transfection efficiency of the buffalo mammary epithelial cells associated with using lipid-based methods or electroporation. Moreover, we preliminary developed an efficient technique for multiple-locus gene targeting at repeated sequences of the buffalo genome.
Non-leaching and durable antibacterial textiles finished with reactive zwitterionic sulfobetaine
Liang He,Chang Gao,Sha Li,Cordelia T.W. Chung,John H. Xin 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.46 No.-
An antibacterialfinishing protocol using a reactive sulfobetaine was reported to fabricate safe anddurable antibacterial textiles. The specifically designed sulfobetaine contains highly reactive triazinegroup acting as an anchor tofix the antibacterial betaine group on textiles via covalent bond formation. After thefinishing, the textiles were endowed durable antibacterial activities of 98.0% against gramnegativeEscherichia coli and 95.2% against gram-positive Staphylococcus aureus even after they werelaundered for 30 times. The safety evaluation showed that the reactive sulfobetaine had no skin irritationand cytotoxicity. These results indicate that the developed antibacterialfinishing is safe and durable ontextiles.
Genetic Detection of Clubroot Resistance Loci in a New Population of Brassica rapa
Wenxing Pang,Shan Liang,Xiaonan Li,Pengpeng Li,Sha Yu,임용표,Zhong Yun Piao 한국원예학회 2014 Horticulture, Environment, and Biotechnology Vol.55 No.6
Clubroot is one of the most serious diseases affecting Brassica crop production worldwide. In order toidentify the location of clubroot resistance genes in Chinese cabbage, we constructed a linkage map for an F2 populationderived from a cross between a resistant turnip inbred line, ‘Siloga’ (B. rapa ssp. rapifera), and a susceptible Chinesecabbage inbred line, ‘BJN3’ (B. rapa ssp. pekinensis). The newly developed genetic map included 207 markers andcovered 1151.7 cM. In combination with clubroot resistance tests from the field, the data allowed us to identify threequantitative trait loci of clubroot resistance, using a composite interval mapping method. Clubroot resistance genesQS_B1.1, QS_B3.1, and QS_B8.1 were located on chromosomes A1, A3, and A8, respectively, of B. rapa. Theircontribution rates were 8.18%, 70.55%, and 7.28%, respectively. QS_B1.1 was a novel locus of clubroot resistance,independent of any published clubroot-resistance loci. QS_B3.1 was first detected in ‘Siloga,’ mapped to the previousCRb and CRa region, whereas QS_B8.1 was closely linked to Crr1b. Epistatic interactions with additive effects weredetected between QS_B3.1 and QS_B8.1.
Huiying Cui,Feng Liang,Chao Ma,Ningning Zhong,Yuliang Sha,Wei Ma 한국지질과학협의회 2019 Geosciences Journal Vol.23 No.4
Although there are many similarities between the shale of Cambrian Qiongzhusi Formation and Ordovician Wufeng Formation–Silurian Longmaxi Formation in South China, including total organic carbon content (TOC) and thickness, the drilling results of shale gas exploration are very different. One of the reasons is the difference of the nano-pores number developed in organic matter between them. In order to reveal the causes, the black shale of Upper Proterozoic Xiamaling Formation in North China, which is similar to the marine source rock in Sichuan basin, was selected for the thermal simulation experiment, and the pore size and volume of the samples before and after the experiment were acquired by scanning electron microscopy (SEM) and nitrogen adsorption isotherm measurement. Through the SEM photographs, we found that the sizes of the organic pores in algae, dispersed organic matter and organic matter associated with clay minerals get bigger with the increasing maturity. The total pore volume, micro-pore volume and meso-pore volume of the shale acquired by nitrogen adsorption isotherm measurement increase with the increasing maturity, too. However, under the overburden pressure, micro-pore volume decreases at high maturity stage, indicating the pores in organic matter might be compressed. It is considered that the pore volume in organic matter of the shale of Qiongzhusi Formation might be compacted under greater confining pressure, which may be the reason why the pore structures of the two sets of marine shale in South China are different.
A new peptide-mediated COF nanocatalytic amplification SERS quantitative assay for ultratrace Cu2+
Yiyi Shu,Shengfu Zhi,Sha Li,Aihui Liang,Zhiliang Jiang 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.113 No.-
Peptides are an important recognition element for small molecules and have been used in the field of biologicalanalysis. However, it has been rarely reported that a novel method combining peptides as recognitionelements with covalent organic framework (COF) as catalytic amplification signal to constructhighly sensitive and selective three-mode molecular spectroscopy. In this paper, high catalytic and stablethree COFs were prepared, and the catalysis of the new indicator nanoreaction of HAuCl4-sodiumformate(Fo) was studied by molecular spectral slope procedure. The produced gold nanoparticles (AuNPs) exhibita strong resonance Rayleigh scattering (RRS) peak at 370 nm and surface plasmon resonance absorption(Abs) peak at 540 nm. In the presence of molecular probes, a strongest surface-enhanced Raman scattering(SERS) peak was generated at 1617 cm1. Combining the COFTpBD amplification indicator reactionwith specific peptide (PT) reaction of Cu2+, a novel, sensitive and selective SERS/RRS/Abs assay platformwas established, with a SERS linear range of 0.005–0.115 nmol/L Cu2+. In addition, the two workingcurves of lg[Cu2+] vs SERS and [Cu2+] vs SERS intensity, and the nanocatalytic mechanism were discussed.
( Xiaoming Pan ),( Yanfang Zhang ),( Xuejiao Sha ),( Jing Wang ),( Jing Li ),( Ping Dong ),( Xingguo Liang ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.3
White spot syndrome virus (WSSV) is a major threat to the shrimp farming industry and so far there is no effective therapy for it, and thus early diagnostic of WSSV is of great importance. However, at the early stage of infection, the extremely low-abundance of WSSV DNA challenges the detection sensitivity and accuracy of PCR. To effectively detect low-abundance WSSV, here we developed a pre-amplification PCR (pre-amp PCR) method to amplify trace amounts of WSSV DNA from massive background genomic DNA. Combining with normal specific PCR, 10 copies of target WSSV genes were detected from ~10<sup>10</sup> magnitude of backgrounds. In particular, multiple target genes were able to be balanced amplified with similar efficiency due to the usage of the universal primer. The efficiency of the pre-amp PCR was validated by nested-PCR and quantitative PCR, and pre-amp PCR showed higher efficiency than nested-PCR when multiple targets were detected. The developed method is particularly suitable for the super early diagnosis of WSSV, and has potential to be applied in other low-abundance sample detection cases.