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Human parvovirus B19 in patients with beta thalassemia major from Tehran, Iran
Seyed Ali Mohammad Arabzadeh,Farideh Alizadeh,Ahmad Tavakoli,Hamidreza Mollaei,Farah Bokharaei-Salim,Gharib Karimi,Mohammad Farahmand,Helya Sadat Mortazavi,Seyed Hamidreza Monavari 대한혈액학회 2017 Blood Research Vol.52 No.1
Background: Due to the tropism of human parvovirus B19 to erythroid progenitor cells, infection in patients with an underlying hemolytic disorder such as beta-thalassemia major leads to suppression of erythrocyte formation, referred to as transient aplasia crisis (TAC), which may be life-threatening. We investigated the prevalence of parvovirus B19 among patients with beta thalassemia major attending the Zafar Adult Thalassemia Clinic in Tehran, Iran. Methods: This cross-sectional study was performed to determine the presence of parvovirus B19 DNA in blood samples and parvovirus B19 genotypes in plasma samples of patients with thalassemia major. The population consisted of 150 patients with beta-thalassemia major who attended the Zafar clinic in Tehran. Specimens were studied using a real-time poly-merase chain reaction assay. Results: The prevalence of parvovirus B19 in our study population was 4%. Of 150 patients with thalassemia, six (4%) were positive for B19 DNA. There was no significant correlation between blood transfusion frequency and B19 DNA positivity. Finally, phylogenetic analy-sis of human parvovirus B19 revealed genotype I in these six patients. Conclusion: In this study, acute B19 infections were detected in patients with beta thalassemia major. Screening of such high-risk groups can considerably reduce the incidence and prevalence of B19 infection; thus, screening is required for epidemiologic surveillance and dis-ease-prevention measures.
Human parvovirus B19 in patients with beta thalassemia major from Tehran, Iran
Seyed Ali Mohammad Arabzadeh,Farideh Alizadeh,Ahmad Tavakoli,Hamidreza Mollaei,Farah Bokharaei-Salim,Gharib Karimi,Mohammad Farahmand,Helya Sadat Mortazavi,Seyed Hamidreza Monavari 대한혈액학회 2017 Blood Research Vol.52 No.1
Background: Due to the tropism of human parvovirus B19 to erythroid progenitor cells, infection in patients with an underlying hemolytic disorder such as beta-thalassemia major leads to suppression of erythrocyte formation, referred to as transient aplasia crisis (TAC), which may be life-threatening. We investigated the prevalence of parvovirus B19 among patients with beta thalassemia major attending the Zafar Adult Thalassemia Clinic in Tehran, Iran. Methods: This cross-sectional study was performed to determine the presence of parvovirus B19 DNA in blood samples and parvovirus B19 genotypes in plasma samples of patients with thalassemia major. The population consisted of 150 patients with beta-thalassemia major who attended the Zafar clinic in Tehran. Specimens were studied using a real-time poly-merase chain reaction assay. Results: The prevalence of parvovirus B19 in our study population was 4%. Of 150 patients with thalassemia, six (4%) were positive for B19 DNA. There was no significant correlation between blood transfusion frequency and B19 DNA positivity. Finally, phylogenetic analy-sis of human parvovirus B19 revealed genotype I in these six patients. Conclusion: In this study, acute B19 infections were detected in patients with beta thalassemia major. Screening of such high-risk groups can considerably reduce the incidence and prevalence of B19 infection; thus, screening is required for epidemiologic surveillance and dis-ease-prevention measures.
Maryam Yaldagard,Seyed.Ali. Mortazavi,Farideh. Tabatabaie 한국화학공학회 2008 Korean Journal of Chemical Engineering Vol.25 No.3
The effects of ultrasound as emerging technology along with thermal treatment were investigated on the activity of barley’s alpha-amylase after germination. All experiments were carried out at 20 kHz on an ultrasonic generator by considering the three effective factors, temperature (30, 50 and 70 oC) and ultrasonic intensities (20, 60 and 100% setting from total power of device (460 W)) in different time intervals (5, 10 and 15 min). For determining the effects of these parameters, the enzymatic activity was assayed by measuring the reducing sugars released as a result of the alpha-amylase action on soluble starch using 3,5-dinitrosalicylate regent (DNS). The results of these assays were analyzed by Qualitek4 software by using the Taguchi statistical method to evaluate the factor’s effects on the enzyme activity. Consequently, the results of assays showed that the activity of this enzyme from germinated barley was reduced after thermosonication by comparing to the blank.
PCR-based Identification of Aflatoxigenic Fungi Associated with Iranian Saffron
Reihaneh Noorbakhsh,Ahmad Reza Bahrami,Seyed Ali Mortazavi,Bita Forghani,Maesoomeh Bahreini 한국식품과학회 2009 Food Science and Biotechnology Vol.18 No.4
Aflatoxins are secondary metabolites produced by the aflatoxigenic fungi in suitable conditions. Saffron, Crocus sativus, is the most expensive spice in the world. Saffron is normally contaminated with soil and hand microflora during harvest and post-harvest operations. In this study, rapid assessment of aflatoxigenic fungi in saffron was accomplished using polymerase chain reaction. In total, 37 market samples were assayed in order to isolate aflatoxin-producing fungi. The 18.9% of the total samples were contaminated with aflatoxigenic fungi. Our results also show that most of the isolated fungi were saprophytes which are normally originated from soil during harvest and postharvest process.
Majid Nooshkam,Fereshteh Falah,Zahra Zareie,Farideh Tabatabaei Yazdi,Fakhri Shahidi,Seyed Ali Mortazavi 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.6
Chitosan (1%) was glycated with inulin (0.5, 1,and 2%) via the Maillard reaction at various initial pHvalues (5, 5.5, and 6). Higher pHs led to a greater pH dropand increase in the intermediate products and browningintensity (BI). The chitosan–inulin conjugates were thenclassified into three levels of low, medium, and high BIthrough K-means clustering in order to investigate theeffect of BI development on the antioxidant and antimicrobialattributes of the conjugates. Covalent linkagebetween chitosan and inulin was confirmed by fouriertransform infrared spectroscopy. High BI chitosan–inulinconjugate had significantly higher antioxidant propertycompared to chitosan and other conjugate fractions. Inaddition, the conjugates obtained at low pH values mainlypresented greater antimicrobial activities than those preparedat high pHs. It can be concluded that chitosan–inulinMaillard-born conjugates can be used as novel antioxidantand antimicrobial prebiotic-based ingredients for foodapplications.