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Lee, Seunghan,Lee, Sunhee,Yoon, Yeo Joon,Lee, Jinwon Humana Press 2013 Applied biochemistry and biotechnology Vol.169 No.2
<P>The goal of this study was to increase the production of long-chain fatty acids and to change the composition of fatty acids through the overexpression of genes involved in the fatty acid synthase (FAS) pathway and utilizing characteristics of a specific gene, namely, fabF. The four genes, fabB, fabG, fabZ, and fabI, are Escherichia coli homologues and function in the elongation cycle of fatty acid biosynthesis. FabB (fabB), an activator of FAS, is a β-oxoacyl-ACP synthase, which catalyzes the addition of acyl-ACP to malonyl-ACP to generate β-oxoacyl-ACP. FabF (fabF) participates at the same step as FabB in the elongation cycle and is structurally and functionally similar to FabB. Hence, we attempted to see if FabF was an activator of FAS, like FabB, with the rationale that these two enzymes have striking similarities. FabF exhibits thermal regulation in that enzyme activity increases at lower temperatures. To confirm its role as an activator of FAS, fabF was overexpressed solely or with other genes in the elongation cycle through biochemical engineering. The fabF recombinants were cultured at different temperatures, resulting in increased total and unsaturated fatty acid accumulation in all the recombinants, compared to wild type, at lower temperatures.</P>
사료내 약용작물 부산물이 가숭어(Liza haematocheila)의 성장, 혈액성상, 면역반응에 미치는 영향
이봉주 ( Bong-joo Lee ),김민기 ( Min-gi Kim ),허상우 ( Sang-woo Hur ),이승형 ( Seunghyung Lee ),이진혁 ( Jinhyeok Lee ),김현종 ( Hyeon Jong Kim ),김이경 ( Yikyung Kim ),이승한 ( Seunghan Lee ),김강웅 ( Kang-woong Kim ) 한국수산과학회 2021 한국수산과학회지 Vol.54 No.4
By-products produced from medicinal plants (MP) were evaluated as feed additives on growth performance, blood chemistry, and immune responses of the juvenile red lip mullet Liza haematocheila. A commercial diet was used as the control. Four experimental diets were prepared by combining 0.5% of MPs such as Panax ginseng (PG), Schisandra chinensis (SC), Angelica gigas (AG), and Rehmannia glutinosa (RG), via water absorption, to the control diet. Triplicate groups of fish averaging 61.8 g were fed one of experimental diets to apparent satiation for 8 weeks. Dietary supplementation of MPs did not affect the growth performance, biological indices and whole body composition of fish compared to the control. The fish fed with AG diet had an increased weight gain, and specific growth rate compared to fish in SC group. Decreased level of plasma glucose was observed in fish fed diet containing MPs. Lysozyme activity was significantly increased in the fish fed AG diet compared to that of fish in PG group. These results indicate that dietary supplementation of Angelica gigas may enhance growth performance and immune responses in juvenile red lip mullet.
Lee, Seunghan,Kim, Kichoon,Lee, Wonchoel Pensoft Publishers 2014 ZooKeys Vol.445 No.-
<P>Abstract</P><P>A new species of the genus <I>Harpacticella</I> Sars, 1908 is described from a tidal pool on Jeju Island, Korea. <I>Harpacticellajejuensis</I><B>sp. n.</B> is closely related to <I>Harpacticellaitoi</I> Chang & Kim, 1991, with regard to the structure of P1 exp-1 and enp-1, the length of P1 exp-1 and exp-2, and the setal number of the P5 exopod in males. However, the new species is clearly distinguishable from <I>Harpacticellaitoi</I> by the combined following characters: six setae on the P5 exopod in females, one naked seta on the inner margin of P1 exp-2, the short endopod of P1 compared to the exopod, and a naked long seta on the proximal inner margin of the P5 exopod of males. The mtCOI partial sequence is provided as a DNA barcode for the new species.</P>
Phase‐contrast hard X‐ray microscopy using synchrotron radiation for the diagnosis of onychomycosis
Lee, Onseok,Ha, Seunghan,Lee, Gunwoo,Kim, Jaeyoung,Huang, Jungyun,Jin, Kyeongsik,Oh, Chilhwan Wiley Subscription Services, Inc., A Wiley Company 2010 Microscopy research and technique Vol.73 No.12
<P><B>Abstract</B></P><P>Onychomycosis, or fungal infection of the nail, is a disease seen frequently in clinical settings. However, the rates of positive identification using potassium hydroxide preparations or fungal cultures are relatively low. Precise diagnosis is possible via histopathologic examination to monitor the existence of fungus and performance of a fungal culture for confirmation. Phase‐contrast hard X‐ray microscopy using synchrotron radiation provides 70‐nm spatial resolution and enables imaging of minute internal cellular structures. This study confirms the feasibility of diagnosing onychomycosis using a phase‐contrast hard X‐ray microscope developed at 1B2 beam line using a Pohang light source. Microsc. Res. Tech. 73:1110–1114, 2010. © 2010 Wiley‐Liss, Inc.</P>