Differential Effects of Pioglitazone in the Hippocampal CA1 Region Following Transient Forebrain Ischemia in Low- and High-Fat Diet-Fed Gerbils.

Moon, Seung Myung,Choi, Goang-Min,Yoo, Dae Young,Jung, Hyo Young,Yim, Hee Sun,Kim, Dae Won,Hwang, In Koo,Cho, Byung Moon,Chang, In Bok,Cho, Sung-Min,Won, Moo-Ho Kluwer Academic/Plenum Publishers 2015 Neurochem Res Vol.40 No.5

<P>In the present study, we investigated the effects of pioglitazone (PGZ) in the hippocampal CA1 region of low- or high-fat diet (LFD or HFD) fed gerbils after transient forebrain ischemia. After 8 weeks of LFD or HFD feeding, PGZ (30 mg/kg) was intraperitoneally administered to the gerbils, following which ischemia was induced by occlusion of the bilateral common carotid arteries for 5 min. Administration of PGZ significantly reduced the ischemia-induced hyperactivity 1 day after ischemia/reperfusion in both LFD- and HFD-fed gerbils. At 4 days after ischemia/reperfusion, the neurons were significantly reduced and microglial activation was observed in the hippocampal CA1 region in LFD- and HFD-fed gerbils. The microglial activation was more prominent in the HFD-fed gerbils compared to the LFD-fed gerbils. Administration of PGZ ameliorated ischemia-induced neuronal death and microglial activation in the hippocampal CA1 region 4 days after ischemia/reperfusion in the LFD-fed gerbils, but not in the HFD-gerbils. At 6 h after ischemia/reperfusion, tumor necrosis factor-α (TNF-α) and interlukin-1β (IL-1β) levels were significantly increased in the hippocampal homogenates of LFD-fed group compared to control group, and HFD feeding further increased TNF-α and IL-1β levels. PGZ treatment significantly ameliorated the increase of TNF-α and IL-1β levels in LFD-fed gerbils, not in the HFD-fed gerbils. At 12 h after ischemia/reperfusion, superoxide dismutase (SOD) and malondialdehyde (MDA) levels in hippocampal homogenates were significantly increased in the LFD-fed group compared to the control group, and HFD feeding significantly showed relatively reduction in SOD activity and increase in MDA level. PGZ administration significantly reduced the increase in MDA levels 12 h after ischemia/reperfusion in the LFD-fed gerbils, but not in the HFD-fed gerbils. These results suggest that PGZ ameliorates the neuronal damage induced by ischemia by maintaining the TNF-α, IL-1β, SOD and MDA levels in LFD-fed gerbils. In addition, HFD feeding affects the modulation of these parameters in the hippocampus after transient forebrain ischemia.</P>

치과용 임플란트 적용을 위한 RBM과 MAO 표면처리 기법을 이용한 티타니아-하이드록시아파타이트 복합코팅

문승균(Seung-Kyun Moon),강민경(Min-Kyoung Kang),조유정(Yu-Jeong Cho),박정종(Jeong-Jong Park),김광만(Kwang-Mahn Kim),김경남(Kyoung-Nam Kim) 대한치과재료학회 2010 대한치과재료학회지 Vol.37 No.2

This study was performed to examine the effect of a composite coating of resorbable blast media (RBM) and micro arc oxidation (MAO) on the bioactivity and cell compatibility. The RBM treatment was performed by grit blasting with 45 micron-sized hydroxyapatite (HA) powder, and micro arc oxidation (MAO) was performed by the electrolyte solution of β-glycerophosphate and calcium acetate on the condition of various applied voltages (100 V - 250 V) for 3 min. After RBM treatment, the modified surface were analyzed by FE-SEM, EDS and FT-IR. After anodic oxidation at 150 V and 200 V, the surface treated by composite coating included HA and TiO₂, but TiO₂ was only found after micro arc oxidation at 250 V. Overall, a composite coating containing HA and porous TiO₂ can be prepared by using RBM treatment and MAO.

실내 스모그 챔버의 설계 및 성능평가

배귀남,김민철,이승복,송기범,진현철,문길주 한국대기환경학회 2003 한국대기환경학회지 Vol.19 No.4

A multi-functional indoor smog chamber was designed and evaluated lo investigate photochenical or water vapor reaction mechanisms of air pollutants. Various smog chamber experiments could be conducted using ambient air or purified air in this smog chamber. The smog chamber consisted of a housing, a Teflon bag, blacklights, injection ports, sampling ports, and utility facilities. The characteristics of light source, the wall losses of air pollutants, and the quality of purified air were experimentally investigated. The maximum NO₂ photolysis rate was 1.10min^(-1). In a 2.5-m³ Teflon bag, the wall losses of ambient O₃, NO, and NO₂were 1.2~2.4×10^(-3)min^(-1), 0.7~2.0×10^(-3)min^(-1), and 0.4-2.0×10^(-3)min^(-1), respectively. The wall loss of ambient particles ranging 0.05 to 0.2㎛ was 1.8~5.4×10^(-3)min^(-1), which was slightly higher than those of ambient gaseous species. The purified air supply system provided high quality of air with NO₂, < 1 ppb, and total hydrocarbons <5 ppb.

개의 췌도분리에서 췌장의 팽창과 콜라겐 분해요소

이상훈,김성주,박정환,김종성,오승훈,한진수,정인경,양태영,김동준,김광원,이문규,민용기,이명식,정재훈,함종렬 대한당뇨병학회 2000 Diabetes and Metabolism Journal Vol.24 No.2

Backgrounds: One of the main problems conditioning the outcome of islet transplantation is the ability to separate a sufficient number of viable islets with preserved function. Islet purification is critically affected by all of the isolation stages. Thus, it is necessary to set up the standard isolation method that islets are separate well from acinar without compromising islet yield and viability. Methods: Twenty three adult mongrel dogs were used for the experiment of total pancreatectomy with islet isolation. The islets were properly isolated by a modified Recordi method. The obtained islets were further purified by centrifugation on discontinuous gradients using cell separation system (Model 2991, Cobe, Lakewood Colo). We evaluated islet number (islet equivalent number, 150 um equivalents/kg of recipient body weight, IEq/kg), purity, cell volume, viabilty, recovery rate, and comparison of outcome according to the isolation conditions. Results: 1) The mean of islet numbers before purification were 13543±9431Eq/kg, digestion times were 13.8±2.6 min., digestion temperature was 37.4±0.2℃, purity was 59.7?.0%, viability was 90.0±2.1%, cell volume was 4.7±1.1 mL, islet number after purification were 4064±361 IEq/kg, and recovery rate was 29±2.9%. 2) Isolated islet numbers were different according to the degree of pancreas distension with collagenase, digestion temperature, and digestion time. 3) The best conditions for islet isolation were above 37.5℃ in temperature at recirculation of collagenase, within 12 min in digestion time and well distended pancreas with collagenase. 4) According to multiple regression adjusted by variable factors, the degree of pancreas distension with collagenase and digestion time were independently associated factors for successful islet isolation. Conclusions: In this study, we concluded that the degree of pancreas distension with collagenase and digestion time were independent factors for successful islet isolation and the best conditions for islet isolation were above 37.5℃ in temperature at recirculation of collagenase, within 12 min in digestion time and well distended pancreas with collagenase.

농업교사의 전문성 요인 척도 개발

문승태,김민배 한국농업교육학회 2007 농업교육과 인적자원개발 Vol.39 No.2

불멸화된 악성구상상피에 대한 Retinoic Acid의 세포활성 및 유전자 발현 연구

문철,민승기 원광대학교 치의학연구소 2003 圓光齒醫學 Vol.12 No.1

cDNA microarray allows to monitor the expression of thousthand genes simultaneously and has been successfully to explore the gene expression of carcinoma and other diseases. To identify and monitor gene expression profile changes in oral tumorigenesis and exposure to medicine may shed light not only on the cause of these pathological changes, but also provide the opportunity to identify novel targets for disease detection and intervention. Retinoids and interferon are important regulators of human epithelial cell differentiation and have been used successfully in the clinical treatment of HPV-involved cervical cancer. However, there is only a limited information available concerning to the effect or role of retinoic acid in oral precancerous and cancer cells. We studied the effect of retinoic acid on normal human oral keratinocyte(NHOK), HPV-16 E6E7 immortalized human oral keratinocyte(IHOK), and two oral squamous cell line(HNSCC30, HNSCC31) according to the carcinogenesis stage. The RA effect was evaluated by MTT assay and microarray. Complementary DNA microarray including 400 genes was used to systematically characterize the variation in gene expression patterns of RA treated IHOK cells vs. control IHOK cells . The results were as follows: 1. RA has negative effect on growth for all NHOK, IHOK, HNSCC30 and HNSCC31 dependently on the concentration and cultivation time. 2. We identified 18 clones cDNA exhibiting more than 2 fold overexpression in RA treated IHOK probes relative to control probe, 21 cDNA s reveal more than 2 fold overexpression in control IHOK relating to the experimental cells. Pearson correlation coefficients for the set of selected spots from duplicated hybridization was 0.86. 3. Examination of gene expression that differs between RA treated IHOK and control IHOK cells apprear to be related to : cell cycle regulator, cell growth and apoptosis, cell adhesion, signal transduction and miscellanous. 4. Cell cycle regulator uprgulation is noted in MAD2, CDK9, Cdc20, Cdc2, CDK inhibitor 1A, and only HUS-1 is downregulated. 5. Cytochrome P450 and interleukin 1-alpha for cell growth and apoptosis factor was upregualted, and capase 8 & FADD apotosis regualtor gene was decreased expression, so we observed increased apoptosis mecahnism and resistance to apoptosis. 6. Cytokine upregulation in RA treated IHOK was noted in interIeukin 1 alpha, downregualtion was in interleukin 8, interleukin 10, interleukin 10 receptor, interleukin 3 receptor, interleukin 9 receptor, and interleukin 19 7. Signal transducers including JAK binding protein, STAT1, and Burkitt lymphoma receptor were upregulated with RA reated IHOK , suggesting the RA regulates the JAK/STAT signal-transduction pathway. In conclusion, we have demonstrated the high-throughput utility of cDNA array hybridization in parallel to the gene expression analysis to identify genes that are expressed differentially in RA treated with HPV16 immortalized keratinocytes. The differentially expressed genes identified here should be informative in oral epithelial cell carcinogenesis.

젊은 성인에서 신체 만족도에 따른 문제해결양식, 대인관계문제와 우울증상과 자살사고와의 관련성

차승민,김태성,강문희,권명진,김정란,왕성근,지익성 대한생물치료정신의학회 2011 생물치료정신의학 Vol.17 No.2

Objectives:The aim of this study was to evaluate that problem solving styles, interpersonal problems, depression and suicidal ideation according to body image satisfaction. Methods:One hundred and nineteen young healthy normal subjects filled out administered the Body Dysmorphic Disorder Examination-Self Report (Korean version of BDDE-SR K-BDDE-SR), Problem Solving Style Scale(Korean version PSS), Korean Inventory of Interpersonal Problem(KIIP), Korean version of the Depression Anxiety Stress Scale(KDASS-21), Korean version of Beck Scale for Suicidal Ideation(Korean version SSI) and Korean version of Reynolds Suicidal Ideation Questionnaire(Korean version SIQ) for a month. Results:Out of the 119 subjects, 39%(N=46) was male and 61%(N=73) was female. In high K-BDDE-SR group (higher than 67), helplessness was significantly higher than low K-BDDE-SR group(lower than 67), and problem-solving confidence, creative style, approach style were significantly lower than low K-BDDE-SR group. Also, Higher level of all KIIP factors in High K-BDDE-SR group was significantly differ than low K-BDDE-SR group. In High K-BDDE-SR group, K-DASS-21, SSI, and SIQ were significantly higher than low K-BDDE-SR group. Using multiple regression analysis contributing variance toward the total factor score of K-BDDE-SR of participants were K-DASS-21 and SIQ. Conclusion:Our results indicates that high tendency of dissatisfied body images have differences in helplessness, problem-solving confidence, approach style, interpersonal problems, depression and suicidal ideation compared to low tendency of dissatisfied body images. It is necessary to study body dysmorphic disorder patients group in the future.

닭의 乾燥 筋胃粘膜 粉末 投與가 Egg Type Fowl에 미치는 效果에 對한 硏究 : 第Ⅲ報 初産日令 및 質에 미치는 影響에 對하여 Ⅲ. The effect of First laying age in a day egg quility

文勝式,朴玉潤,宋瑛敏 진주산업대학교 1982 論文集 Vol.20 No.-

This experiment was conducted to investigate the effects of the additions at the level of 0(C), 1(T_1), 2(T_2) and 3%(T_3) of Fowl Gizzard Mucouse Membrane Powder in the formula feed on the average day at first laying and egg quality with a total of 40 pieces laying hens for 4 treatments during the experimental period of 84 days from Feb.1, 1982 to April 25, the same year at the brooding laboratory, Dept. of Animal Science, Jin Ju Agricultural and Forestry Junior Technical College. The average obtained are summarized as follows: 1. The average day at first laying day was long in the order of T_1(158.8±8.30), T_2(159.9±10.13), T_3(162.6±3.53) and C(164.3±7.04) and the average weight at first laying(kg), heavy in the order of T_1(2.10±0.16), T_2(2.11±0.19), T_2(2.13±0.08) and C(2.27±2.25)(P>0.05). The egg weight at first laying(g) was heavy in the order of T_2(47.5±5.09), T_3(48.15±4.13), T_1(48.9±4.93) and C(51.99±2.13)(P>0.05) 2. The average egg weight(g) was heavy in the order of C(59.66±2.06), T_1(59.74±2.56), T_2(60.70±5.23) and T_3(62.64±3.67) and the egg shell, thickness in the order of T_3(0.362±0.036), C(0.370±0.038), T_1(0.378±0.022) and T_2(0.382±0.034)(P>0.05). The yolk and albumen weight(g) was heavy in T_2(18.20±1.57), C(18.35±.95), T_3(18.60±0.97) and T_2(18.92±1.05), T_1(33.61±1.94), C(34.32±1.65), T_2(35.37±3.77) and T_3(36.64±2.62) in those order(P>0.05).

艾葉粉末의 添加給與가 肉鷄에 미치는 影響

文勝式,朴玉潤,宋瑛敏 진주산업대학교 1985 論文集 Vol.23 No.-

本 試驗은 1985년 9월 20일 孵化된 肉鷄用(Hubbard) 병아리 120首를 各區當 30首씩 4개區로 나누어 晋州農林 專門大學 養鷄場에서 3주간(1985.10.19~11.8)에 걸쳐 艾葉을 C(0%), T_1(1%), T_2(2%), T_3(3%)水準으로 飼料에 添加하여 給與試驗을 하였던 바 다음과 같은 성적을 얻었기에 報告하는 바이다. 1. 增體量은 T_1區(1296.32g), C區(1274.70g), T_3區(1264.32g) 및 T_2區(1259.64g)의 순으로 나타났다(p>0.05). 2. 飼料攝取量은 C區(2715. 13g), T_1區(2738.60g), T_2區(2754.16g) 및 T_3區(2811.46g)의 순으로 나타났고 飼料要求率은 T_1區(2.113), C區(2.130), T_2區(2.178) 및 T_3區(2.232)의 순으로 나타났다(p>0.05). 3. 産肉指數는 T_1區(306.48), C區(303.01), T_2區(293.38) 및 T_3區(284.86)의 순으로 나타났다. 4. 屠體率은 ♀는 T_1區(69.45), T_2區(69.30), T_3區(67.58) 및 C區(66.45)의 순이였고 ♂은 T_3(70.03), T_2區(69.13), T_1區(67.85) 및 C區(66.58)의 순이였다. 5. 臟器重量은 대조구와 처리구간에 차이를 볼 수 없었다. 6. 經濟性을 분석한 結果 各 區別 수당수익은 T_1區(613원), C區(590원), T_2區(583원) 및 T_3區(572원)의 순으로 나타났다. This experiment was all located to 4 plots by 30 chickens per each plot with 120 chickens of broiler-Hubbard hatched at the 20th of September, 1985 and the formula feed added in the levels of 0(C plot), 1(T_1 plot), 2(T_2 plot) and 3%(T_3 plot) of Artemisia was supplied for these of each plot during 3 weeks from October, 19 to November, 8, 1985 at the poultry house, Chinju Agricultral & Forestry Technical College. The results found out the effect of Artemisia meal addition on the growth of broiler-Hubbard were as follows: 1. The weight gain(g) was heavy in the order of T_1(1296.32), C(1274.70), T_2(1264.32) and T_3(1259.64) and non significant (p>0.05) between plots. 2. Feed consumption(g) was heavy in the order of C(2715. 13), T_1(2738.6), T_2(2754.16) and T_3(2811.46) and feed conversion, T_1(2.113), C(2.130), T_2(2.178) and T_3(2.232) (p>0.05). 3. Point spread was high in the order of T_1(306.48), C(303.01), T_2(292.38) and T_3(284.86). 4. Dressing percent of female ws high in the order of T_1(69.45), T_2(69.30), T_3(67.58) and C(66.45), and of male, T_3(70.03), T_2(69.13), T_1(67.85) and C(66.58). It was generally higher in the Artemisia meal addition plot than in the control. 5. Internal organies weight was non significant differences(p>0.05) between treatment plots and control. 6. In economical analysis, net income(won) per bird of each plot was 613(T_1), 590(C), 583(T_2) and 572(T_3).

전경대원에서 발생한 결핵의 임상 양상

문창기,박상준,조민구,김영중,김소연,김윤권,정준오,안석진,김은실,서승오,김지훈,최원제,이윤영,박형기,최규영,김현근 대한감염학회 2006 감염과 화학요법 Vol.38 No.6

Background: The purpose of this study was to document the incidence and clinical characteristics of patients with tuberculosis (TB) in combat and auxiliary police, living in a group, in Korea where the incidence rate of active TB in a general population is higher than in Western countries. Materials and Methods:We retrospectively reviewed the medical records of all tuberculosis patients diagnosed at National Police Hospital from January 2002 through December 2004. Results:In 2002-2004, a total of 156 cases of tuberculosis were identified with the mean (Standard deviation) age of 20.6 (±1.0) years. Of these, 134 (85.9%) patients were registered as new cases, 11 (7.1%) as relapse, 2 (1.3%) as failure, 5 (3.1%) as treatment-after-default cases whereas 4 (2.6 %) patients were not included in any categories. Average annual new TB rate and smear-positive TB rate were 86.5/10^(5) and 17.4/10^(5) person-years, respectively. In 12 of 31 smear-positive cases, time from onset of symptoms to diagnosis was more than 30 days. Two multidrug-resistance TB cases were identified and two suspected outbreak episodes of TB had occurred during 3 years. Conclusion:There was no statistically significant difference in the incidence rate of new cases of TB between the general population aged 20 to 29 years and combat and auxiliary police in Korea. 배경 : 국내 결핵의 발생은 감소 추세에 있으나 여전히 후진국형 발생양상을 보이고 있다. 특히 집단생활을 하는 젊은이들에서 발생하는 결핵의 유행이 간헐적으로 알려지고 있어 우려를 낳고 있다. 이에 저자들은 최근 전경대원의 결핵 발병 양상에 대해 알아보고자 본 연구를 시행하였다. 재료 및 방법 : 2002년 1월부터 2004년 12월까지 3년간 경찰병원에서 결핵 진료를 받은 전경대원의 의무기록을 후향적으로 분석하여 연도별 결핵 신환발생률, 도말양성결핵 신환율, 진단 지연, 집단발병, 다제내성결핵을 조사하였다. 결과 : 연도별 결핵 신환자 수(10만명당 발생추정치)는 2002년 50명(83.6명), 2003년 42명(83.7명), 2004년 42명(93.5명)이었고 도말양성 폐결핵 신환자 수는 2002년 10명(16.7명), 2003년 11명(21.9명), 2004년 6명(13.4명)이었다. 3년간 도말양성 폐결핵 환자는 31명이 있었고 그 중 12명은 호흡기 증상 발생 30일이 지나서 진단을 받았다. 다제내성 결핵은 2명이 있었으며 결핵의 집단발병이 의심되는 사례는 2차례 있었다. 결론 : 전경대원과 20-29세 연령군 일반인의 결핵 신환발생률, 도말양성 폐결핵 발생률의 통계적으로 유의한 차이는 없었다.