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Enhancing Fatty Acid Production of <i>Saccharomyces cerevisiae</i> as an Animal Feed Supplement
You, Seung Kyou,Joo, Young-Chul,Kang, Dae Hee,Shin, Sang Kyu,Hyeon, Jeong Eun,Woo, Han Min,Um, Youngsoon,Park, Chulhwan,Han, Sung Ok American Chemical Society 2017 Journal of agricultural and food chemistry Vol.65 No.50
<P><I>Saccharomyces cerevisiae</I> is used for edible purposes, such as human food or as an animal feed supplement. Fatty acids are also beneficial as feed supplements, but <I>S. cerevisiae</I> produces small amounts of fatty acids. In this study, we enhanced fatty acid production of <I>S. cerevisiae</I> by overexpressing acetyl-CoA carboxylase, thioesterase, and malic enzyme associated with fatty acid metabolism. The enhanced strain pAMT showed 2.4-fold higher fatty acids than the wild-type strain. To further increase the fatty acids, various nitrogen sources were analyzed and calcium nitrate was selected as an optimal nitrogen source for fatty acid production. By concentration optimization, 672 mg/L of fatty acids was produced, which was 4.7-fold higher than wild-type strain. These results complement the low level fatty acid production and make it possible to obtain the benefits of fatty acids as an animal feed supplement while, simultaneously, maintaining the advantages of <I>S. cerevisiae</I>.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jafcau/2017/jafcau.2017.65.issue-50/acs.jafc.7b04485/production/images/medium/jf-2017-044858_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/jf7b04485'>ACS Electronic Supporting Info</A></P>
대량 출혈로 발현한 원발성 위장관 말초 T세포 림프종 1예
김유선,송인성,정현채,김상균,김우호,김정룡,김주성,천재희,서승오,박규주 대한소화기학회 2000 대한소화기학회지 Vol.36 No.2
Most primary gastrointestinal lymphomas are B-cell origin. T-cell lymphomas of the intestine comprise the small portion of gastrointestinal lymphomas. Peripheral T-cell lymphomas (PTCL) rarely affect the gastrointestinal tracts at their initial presentation and the majority of primary gastrointestinal PTCL involve the small intestine. There has been few reports about the diffuse and extensive involvement of gastrointestinal tracts by PTCL. In Korea, there has been no report of a primary gastrointestinal PTCL so far. The common manifestations of PTCL involving the gastrointestinal tracts are abdominal pain, gastrointestinal bleeding, perforation, and diarrhea. However, these are not sufficient for the correct diagnosis. Several other gastrointestinal diseases may mimic PTCL. Its low incidence and clinical similarity to other gastrointestinal diseases make a correct diagnosis difficult. We report a case of primary gastrointestinal PTCL involving the whole gastrointestinal tracts, which rarely casuses massive bleeding, with a review of literatures.
Kang, Dae Hee,You, Seung Kyou,Joo, Young-Chul,Shin, Sang Kyu,Hyeon, Jeong Eun,Han, Sung Ok Elsevier 2018 Bioresource technology Vol.250 No.-
<P><B>Abstract</B></P> <P>In the practice of converting red algae biomass into biofuel or valuable biomaterials, the critical step is the decomposition process of the agarose to give fermentable monomeric sugars. In this study, we selected three enzymes such as agarase, carrageenase and neoagarobiose hydrolase to inducible the simultaneous hydrolysis of the major substrates such as agar and carrageenan constituting the pretreated red algae, and expressed the chimeric enzymes and formed a complexes through optimization of addition ratio. As a result, hydrolysis by enzyme complexes showed a maximum sugar release of 679 mg L<SUP>−1</SUP> with 67.9% saccharification yield from <I>G. verrucosa</I> natural substrate. The difference in the reducing sugar by the enzyme complexes was 3.6-fold higher than that of the monomer enzyme (cAgaB yield 188.6 mg L<SUP>−1</SUP>). The synergistic effect of producing sugars from red algae biomass through these enzyme complexes can be a very important biological tools aimed at bioenergy production.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The <I>Gracilaria cerrucosa</I> was extracted with agar and carrageenan by freezing-thawing. </LI> <LI> Enzyme complexes improved the sugar production ability than mixtures or mono-enzymes. </LI> <LI> The hydrolysis process using an enzyme complexes is efficient for sugar production. </LI> </UL> </P>