Wax‑printed well pads and colorimetric LAMP detection of ApxIA toxin gene

SeonHyung Lee,Ji Hun Kim,Beom‑Ku Han,Won‑Il Kim,Byung‑Kwan Cho,Sung Min Woo,Yang‑Hoon Kim,Ji‑Young Ahn 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.3

Background Actinobacillus pleuropneumoniae (A. pleuropneumoniae) produces hemolytic cytotoxins, ApxI, ApxII, ApxIII, and ApxIV, causing a huge loss to the swine industry. Objective We developed the PadLysis protocol for extracting genomic DNA (gDNA) for the LAMP—LeucoCrystal Violet (LCV) colorimetric detection. A paper-based well pad for gDNA extraction was fabricated using a wax printing method. Furthermore, the LAMP amplicons were detected in situ with colorimetric dye, LCV. Results Wax-printed well pads (WPWPs) have hydrophobic wax barriers by printing a well pattern that allows the establishment of simple and equipment-free PadLysis protocol consisting of the three steps: (1) 2 min lysis, (2) 2 min washing, and (3) 1 min drying. This proposed platform allowed direct LAMP amplification for A. pleuropneumoniae field isolates. We optimized LCV chemical components to enable the accurate LAMP signal readouts. The overall assay takes up to 45 min including 5 min PadLysis, 40 min LAMP amplification, and LCV signal observation with the naked eye. Conclusion Our WPWPs–LAMP–LCV approach can be used as a rapid screening tool for A. pleuropneumoniae pleuropneumonia (APP) caused by ApxIA toxin in future outbreak areas.

An Exploratory Study of Smart Sportswear Segments for Motion Sensors-Focusing on Rowing Motions

( Seonhyung Park ),( Bo Ram Han ),( Bokku Kang ),( Hae Dong Lee ),( Han Sung Kim ),( Joohyeon Lee ) 한국감성과학회 2015 추계학술대회 Vol.2015 No.-

Lots of interdisciplinary studies for fusion of high technologes and the other areas of research had tried in these days. In sports training area, high technologies like a vital sign sensor or an accelerometer were adopted as training tools to improve the performance of the sports players. The purpose of this study is finding the proper locations on the human body for attaching the motion sensors in order to develop a smart sportswear which could be helpful in training players. The rowing was selected as a subject sport as lots of movements of the joint on human body could be seen in rowing motions. The players of rowing could be devide into two weight divisions, the lightweight and the heavyweight. In this study, the rates of changes on human skin as the players moved were took on the back, the elbow, the hip and the knee area on human body by 3D motion capturing system. The distances between markers and he differences between the lightweight and heavyweight were analyzed. Finally, the guide lines for designing a mtion sensing smart sportswear.

Bacterial Cellulose-based Photo-curable 3D Printable Bioink for Biomedical Application

SeonHyung LEE,Yang-Hoon KIM,Ji-Young AHN 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10

Cellulose materials have various advantages such as high surface area, mechanical strength, and biodgradability. For this reason, cellulose materials are widely applied in the biomedical application. In particular, cellulose materials are utilized in biomedical application such as wound healing and tissue engineering using 3D printing. Among cellulosic materials, we made a photo-curable bioink using bacterial cellulose(BC). BC was produced by Gluconacetobacter hansenii and was modified into a bio-ink material through a two-step process of carboxy methylation and methacrylation. Modification of the fabricated material was confirmed by FT-IR, NMR and TGA analysis. I2959 was added to modified methacrylated carboxymethyl cellulose (CMC-MA), which was cured using UV after printing. The optimal concentration of CMC-MA bioink was determined by considering the water absorption capacity of each ink concentration, and the produced CMC-MA bioink was printable and biodegradable. For application to the biomedical field, it was confirmed that there was no effect on the viability of cells both before and after printing and curing by cytotoxicity test. In conclusion, we have developed a BC-based bioink that can be widely applied in the biomedical application due to its advantages such as non-toxic, biodegrable and printable.

코로나-19 무증상 감염 환자의 한약 치료 방안에 대한 고찰

정선형(Seonhyung Jeong),이경은(Kyoungeun Lee),정민정(Minjeong Jeong),송미덕(Mideok Song),장인수(Insoo Jang) 대한한의학회 2021 대한한의학회지 Vol.42 No.1

Zika Virus Detection Using Reverse Transcription-loop-mediated Isothermal Amplification and Lateral Flow Assays

Gna AHN,SeonHyung LEE,Yun Hee BAEK,Min-Suk SONG,Yang-Hoon KIM,Ji-Young AHN 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.4

패션의 눈으로 바라본 웨어러블 컴퓨터

이주현(JooHyeon Lee),박선형(SeonHyung Park) 한국정보과학회 2000 정보과학회지 Vol.18 No.9

Some Biological Consequences of the Inhibition of Na,K-ATPase by Translationally Controlled Tumor Protein (TCTP)

Jung, Jiwon,Ryu, Seonhyung,Ki, In A,Woo, Hyun Ae,Lee, Kyunglim MDPI 2018 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.19 No.6

<P>Na,K-ATPase is an ionic pump that regulates the osmotic equilibrium and membrane potential of cells and also functions as a signal transducer. The interaction of Na,K-ATPase with translationally controlled tumor protein (TCTP) results, among others, in the inhibition of the former’s pump activity and in the initiation of manifold biological and pathological phenomena. These phenomena include hypertension and cataract development in TCTP-overexpressing transgenic mice, as well as the induction of tumorigenesis signaling pathways and the activation of Src that ultimately leads to cell proliferation and migration. This review attempts to collate the biological effects of Na,K-ATPase and TCTP interaction and suggests that this interaction has the potential to serve as a possible therapeutic target for selected diseases.</P>

유턴을 고려한 교통축 적용 수요대응 자율주행 대중교통(DRDTO)의 실시간 동적 경로 생성 알고리즘

김현(KIM, Hyun),유선형(YOO, Seonhyung),이진우(LEE, Jinwoo),백범열(BAEK, Beomyeol),신정희(SHIN, Junghee) 대한교통학회 2022 대한교통학회지 Vol.40 No.2

최근 자율주행 차량이 대중교통분야로 진출하여 관련 연구들이 활발히 진행되고 있다. 본 연구에서는 시공간적으로 다양하게 발생하는 실시간 호출수요에 대응하여 자율주행 차량에 동적 경로를 제시하여 효율적인 대중교통 서비스를 제공할 수 있는 알고리즘을 개발했다. 실시간 호출수요는 다수의 출발지와 다수의 목적지로 구성되어 복잡하고 다양한 경우의 수를 고려하여 최적 경로를 제시할 수 있는 방안이 필수적이다. 복잡한 계산과정은 오프라인 과정에서 선행할 수 있는 방법으로 머신러닝의 강화학습 기술을 사용하여 제시하였다. 개발된 자율주행 대중교통(DTO)를 위한 실시간 호출 수요대응 동적경로 알고리즘은 테스트베드를 선정하여 시뮬레이션 실험이 이루어 졌다. 시뮬레이션 결과는 개발된 실시간 호출 수요대응 동적경로 생성 알고리즘을 고정노선의 운행결과 대비하여 효율적인 운행결과가 나타났다. 이는 실시간 호출수요량이 적을수록 효과가 크게 나타나지만 호출 수요량이 증가하면서 그 효과 폭이 점차적으로 감소했다. Recently, autonomous vehicle is receiving much attention in various sectors including transportation and public transportation. This study presents an adaptive routing algorithm for the real-time demand response service of autonomous transit vehicles. The experimental setting includes the real-time demand occurring randomly over time within the study area. Each demand (service call) request the transit service from designated origin to destination. The routing algorithm is designed to make U-turn and skip stops to improve service and reduce the wait time of users. This study adapts Reinforcement Learning, one of the machine learning techniques, namely reinforcement learning, which can precede a complex calculation process in an offline process. Simulation experiments conducted on a testbed of the Chungju campus of the Korea National University of Transportation. The simulation results show that the proposed routing algorithm can improve the adaptive transit service over the fixed operation in selected performance indicators.

코로나-19 환자의 후유증 한약 치료에 대한 고찰

이경은,정선형,정민정,최유민,송미덕,장인수,Lee, Kyoungeun,Jeong, Seonhyung,Jeong, Minjeong,Choi, Yoomin,Song, Mideok,Jang, Insoo 대한한방내과학회 2021 大韓韓方內科學會誌 Vol.42 No.1

Objective: The purpose of this study was to collect and evaluate evidence of herbal medicine treatments for the residual effects of COVID-19. Methods: Nine electronic databases, namely PubMed, Science Direct, CINAHL, CNKI, Wanfang, J-STAGE, CiNii, OASIS, and Science ON, were searched with the following keywords: 'COVID-19'; 'sequelae'; 'Convalescence' for 'P (Population)' and 'Medicine, Chinese Traditional'; 'Korean medicine'; 'Herbal medicine' for 'I (Intervention)'. For the selected studies, various clinical data were extracted including patient symptoms, pattern identification, herbal medicines prescribed, and treatment results. Results: A total of nine clinical studies were identified (two case control trials; one case series; and six case reports). Various residual symptoms had been detected in the recovery stage of COVID-19 with the most commonly used pattern identifications being deficiency in both lung and spleen and in both qi and yin. Any herbal medicine prescriptions were written using these patterns. We found several reports of the positive effect of herbal medicines in that it improved symptoms, lung function, and lung inflammation. No adverse events were reported. Conclusions: The results indicate that treatment with herbal medicines has a possible therapeutic effect on the residual symptoms of COVID-19. Large-scale studies are necessary to confirm the effectiveness of these approaches.

Digital Coloring Clothing and Emotion

( Jin Hee Yang ),( Hyun Seung Cho ),( Seonhyung Park ),( Joo Hyeon Lee ) 한국감성과학회 2015 추계학술대회 Vol.2015 No.-

In this research, we investigated the luminance efficiency of the computerized embroidery methods applied to the flexible POF for realization of the textile display. Due to the nature of the flexible POF similar to that of textile yarn, it has been enabled to realize a flexible textile display by application of some adjusted fabrication methods to it. However, besides the simple property of flexibility, two requirements are still demanded for the flexible POF-based textile display and emotion: high-luminance and the method for realization of pixels in a display. Among the fabrication methods, the machine embroidery has feasibility to realize relatively higher luminance of the flexible POF-based textiles through a simple procedure. The aim of this study is to evaluate of the feasibility of the embroidery method for the flexible POF-based textile display in terms of luminance, and to approach to the suitable condition in the embroidery method to realize the textile display and emotion. To do this, the four embroidery methods, in which the bending angles from ‘around 90°’ to ‘around 180°’ were applied to the flexible POF, were developed in this study. The luminance at fifteen locations in the bent shapes in each of the four embroidery methods were examined and compared. Based on the resulted luminance efficiency according to the four embroidery methods, the suitable conditions for the embroidery method to realize relatively higher level of luminance for the textile display were suggested.