LC/MS/MS 분석법을 이용한 Amlodipine의 약물동태연구

서정원,윤민혁,강원구,권광일 충남대학교 약학대학 의약품개발연구소 2007 藥學論文集 Vol.22 No.-

The aim of this study were to confirm the analysis method and also to estimate the pharmacokinetic parameters of amlodipine in human volunteers. In an open-label single-dose pharmacokinetic study, a group, consisting of 24 healthy volunteers, received single oral dose of 5mg amlodipine. Blood sample were taken for up to 120 hours. The concentration of amlodipine in these body fluids was determinated using a validated high-performance liquid chromatography(HPLC) method with tandem mass spectrometry. Amlodipine and ketoconazole, an internal standard, were extracted from plasma using ethyl acetate in the presence of 0.1M sodium carbonate. After drying the organic layer, the residue was reconstituted in mobile phase(acetonitrile : water = 70 : 30 v/v (0.1% formic acid)) and injected onto a Zorvax C18 column (2.1 × 100 mm, 3.5 ㎛ particles). The isocratic mobile phase was eluted at 0.2ml/min. The ion transitions monitored in multiple reaction-monitoring mode were m/z 410.10 → 294.95 and 532.11 → 81.95, respectively. The coefficient of variance of the assay precision was less than 12%, and the accuracy exceeded 99.1%. The limit of quantification was 0.1 ng/ml. The pharmacokinetic parameters were calculated with non-compartmental(AUC, C_(max), T_(max), CL_(t), V/F) and compartmental(K_(el), K_(a), t_(lag)) pharmacokinetic analysis using WinNonlin program. The estimated means of AUC_(0-120hr), C_(max) and T_(max) were 196.90 ± 5.02 ng·hr/ml, 3.36 ± 0.09 ng/ml and 10.44 ± 0.61 hr, respectively. The means of other pharmacokinetic parameters(V/F, CL_(t), K_(el), K_(a) and t_(lag)) were 1208.06 ± 50.61 L, 25.39 ± 0.65 L/hr, 0.2806 ± 0.0294 hr^(-1), 0.0210 ± 0.0008 hr-1 and 0.4574 ± 0.0635 hours, respectively.

한국인 관상동맥의 변화양상에 대한 형태학적 연구

권태정,이상용,이한영,고영창,권기석,조재홍,김태공,김유훈,조갑래,서중석,이원태 內務部 國立科學搜査硏究所 2001 國立 科學 搜査 硏究所 年報 Vol.33 No.-

정신분열병 환자에서의 한국어판 사회적응 척도의 개발 : 신뢰도 및 타당도 연구

김철권,서지민,김상수,조진석,하준선,김정옥,변원탄 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.6

연구목적: 정신분열병 환자의 사회적응 정도를 측정하는데 사용되는 사회적응 척도의 한국어판을 개발하기 위함이다. 방 법: 사회적응 척도를 번역한 후 정신분열병 환자 67명과 정상인 78명을 대상으로 신뢰도와 타당도를 검증하였다. 결 과: 자료분석 결과 검사-재검사 신뢰도는 0.85, 검사자간 신뢰도는 0.89, 내적 일치도는 0.87로 높은 수치였다. 변별 타당도를 검증하기 위하여 정신분열병 환자군과 정상 대조군을 비교한 결과 정신분열병 환자군에서 사회적응 정도가 유의하게 낮았다. 구성 타당도를 알아보기 위하여 10개 영역간의 상관관계를 측정한 결과 성적적응 영역을 제외한 모든 영역들에서 유의한 상관관계를 보였다. 본 척도의 전반적 평가 영역 결과와 GAF 결과간의 상관성을 통해 간접적으로 검증한 공존 타당도 역시 유의하였다. 결 론: 한국판 사회적응 척도는 우수한 신뢰도와 타당도가 입증되었고 나아가 정신분열병 환자의 사회적응 정도를 평가하는데 유용하게 사용되어질 수 있을 것이다. Objectives: The purpose of this study was to develop the Korean version of the Social Adjustment Scale Ⅱ-Revised Version(KSAS Ⅱ-RV) in the schizophrenics. Methods: KSAS Ⅱ-RV was administered to 67 schizophrenic patients and 78 matched normal controls for examining the reliability and validity. Results: Data analysis showed statistically significant reliabilities and validities of KSAS Ⅱ-RV. The test-retest reliability, inter-rater reliability, and internal consistency for total scores of KSAS Ⅱ-RV were 0.85, 0.89. and 0.87, respectively. Evidence for discriminant validity of KSAS Ⅱ-RV comes from the results that the mean scores of schizophrenic patients were significantly high than those of normal controls. Construct validity was assessed by calculating the 10 inter-subareas correlations of the KSAS Ⅱ-RV, and all except the sexual adjustment area were statistically significant. Significant correlations between the global area scores of KSAS Ⅱ-RV and those of Global Assessment of Functioning Scale lend support for the concurrent validity of this instrument indirectly. Conclusions: KSAS Ⅱ-RV was found to be a valid and reliable instrument which can be used to evaluate the degree of social adjustment in the schizophrenic patients.

Aspergillus awamori와 Zymomonas mobilis로 구성된 고정화혼합 배양계의 에탄올 생산에 미치는 Triton, PVA 및 PEG의 영향

이상원,서권일,박석규,손봉수,김홍출,성찬기 순천대학교 기초과학연구소 1995 基礎科學硏究誌 Vol.6 No.-

The effects of triton-X 100, PVA and PEG on the ethanol production and raw starch hydrolysis by co-immobilized A. awamori and Z. mobilis (A-Z system) were investigated. When gel beads containing A. awamori(1.25 × 10 exp (9) spores/ℓ - gel) and Z. mobilis(0.5g cell/ℓ - gel) were cultured in 2% raw starch solution at 30℃ for 120 hours, ethanol concentration, ethanol yield (Y_p/s) and pH of culture broth were 2.8g/ℓ, 0.16 and 3.8~4.0, respectively. Ethanol production and starch hydrolysis by addition of PVA was efficient, compared to that of PEG and ethanol concentration in culture broth with 0.2% PVA was 3.56g/ℓ. At A-Z 36 culture system with changing silicon check valve for cotton plug at 36 hours in A-Z culture system, ethanol yield on glucose, Y_p/s = 0.36, showed 2 times higher than that of control culture system(cotton plug culture). Ethanol production and productivity by cultivating 85g gel beads at 30℃ in a 1ℓ jar fermentor(220rpm) of medium 500㎖ were 32g/ℓ and 1.8g/ℓ/day, respectively. After 12 hours of cultivation, gel beads were distinguished oxygen-rich surface for A. awamori from oxygen-deficient central part for Z. mobilis.

정신분열병 환자의 주 보호자와 형제 자매에서의 부담에 대한 연구

김철권,김진원,서지민,이길자,김길중,변원탄 大韓神經精神醫學會 2000 신경정신의학 Vol.39 No.1

연구목적: 정신분열병 환자의 가족 부담에 영향을 미치는 요인들을 알아보기 위함이다. 방 법: 92명의 정신분열병 환자의 주 보호자 92명과 그들의 형제 자매 68명을 대상으로 자가보고 척도를 사용하여 가족부담, 환자의 문제행동, 가족의 죄책감 정도를 평가하였다. 그리고 환자의 양성증상 행동과 음성증상 행동, 환자의 임상적·사회인구학적 변인, 주 보호자의 사회인구학적 변인, 그리고 가족의 죄책감 등이 주 보호자 및 형제 자매의 주관적·객관적 부담에 어떤 영향을 미치는지를 조사하였다. 결 과: 1) 객관적 부담과 주관적 부담에서 주 보호자군이 형제 자매군에 비해 약간 높은 경향을 보였지만 양군간에 유의한 차이가 없었다. 2) 정신분열병 환자의 임상적·사회인구학적 변인들 중에서는 환자의 연령, 유병기간, 총 입원기간이 주 보호자 또는 형제 자매의 부담에 유의한 영향을 주었다. 그리고 주 보호자의 사회 인구학적 변인들 중에서는 매달 수입 정도와 가족교육 여부가 주 보호자의 부담에 유의한 영향을 주었다. 3) 문제행동들을 양성증상, 음성증상, 기타증상으로 분류한 후 주 보호자 및 형제 자매의 객관적 부담과 주관적 부담과의 상관성을 검증한 결과, 양군 모두에서 3가지 증상 행동이 객관적 및 주관적 부담과 각각 유의한 상관성을 보였다. 그리고 양군에서 양성증상 행동과 음성증상 행동이 객관적 부담보다는 주관적 부담과의 상관성이 상대적으로 더 높은 양상을 보였다. 또 형제 자매의 경우에는 양성증상 행동이 음성증상 행동에 비해 객관적 및 주관적 부담과의 상관성이 더 높았다. 4) 죄책감과 부담간의 상관성에서는 주 보호자에서만 유의한 상관성을 보였다. 결 론: 본 연구 결과는 정신분열병 환자의 가족 부담을 줄일 수 있는 보다 효과적인 가족중재 기법을 개발하는데 있어 유용한 자료가 될 것으로 생각된다. In order to investigate the factors associated with family burdens of schizophrenics, 92 primary caregivers and 68 siblings of 92 persons with schizophrenia were examined by means of self-report scales about the effect of positive and negative symptom behaviors, clinical and sociodemographic variables, and guilty feelings toward patient on the objective and subjective burden. The results were as follows. 1) There was no difference in the objective and subjective burden scores between the primary caregivers and siblings, even though the former showed a higher scores compared to the latter. 2) Burden scores were significantly affected by the factors such as age of patients, duration of illness, total duration of hospitalizations, monthly family income, and the experience of family education in the primary caregivers or siblings of schizophrenics. 3) Objective and subjective burden were related to both the severity of positive, negative, and the other symptom behaviors in the primary caregivers and siblings. The positive and negative symptom behaviors were more related to subjective burdens compared to objective burdens in the primary caregivers and siblings. The positive symptom behaviors were more related to perceived objective and subjective burdens compared to negative symptom behaviors in the siblings. 4) The correlations between guilty feelings toward patient and burden scores was significant only in the primary caregivers. These results could be used as useful datas to develop the more effective family intervention for reducing family burden.

한국인 관상동맥의 변화양상에 대한 형태학적 연구

이상용,권태정,정낙은,서중석,이한영,이원태 大韓法醫學會 2002 대한법의학회지 Vol.26 No.1

전처리 돌산갓 추출물의 첨가에 따른 김치의 보존효과

박석규,서권일,이상원,강갑석,손미혜 동아시아식생활학회 1997 동아시아식생활학회지 Vol.7 No.1

To investigate the food preservative effects of leaf mustard Dolsan(Brassica juncea), the pretreated extract of leaf mustard Dolsan were added to Kimchi and the results are as following, pH of Kimchi added leaf mustard Dolsan extracts were lower than that of control at initial stage, but after 6 days of fermentation, pH was higher in order of Kimchi added the pretreated extract of leaf mustard Dolsan(hydrolyzed at 30℃ for 24 hours, PEM), the unpretreated extract of leaf mustard Dolsan(UEM) and control. The total number of bacteria and lactic acid bacteria increased rapidly at the initial stage of fermentation and reached plateau at 2 days of fermentation. The number of bacteria and lactic acid bacteria, of Kimchi added leaf mustard Dolsan extracts were lower than that of control, and antimicrobial activity of PEM in Kimchi was higher than that of UEM. After 2 days fermentation, sensery value of Kimchi added PEM was more excellent than that of control.

돌산갓 전처리 중의 산도, 항균선 및 색도 변화

박석규,서권일,이상원,조영수,손미혜 동아시아식생활학회 1997 동아시아식생활학회지 Vol.7 No.1

In order to use leaf mustard Dolsan food preservative ingradient, acidity, antimicrobial activity and colors were investigated during pretreatment of leaf mustard Dolsan. pH was remarkably decreased after 8 hours to pretreatment(extracted on shaking) of leaf mustard Dolsan, and no changes were observed after that time. pH of leaf part was higher than stalk after 8 hours storage. Titratable acidity was opposite tendency to the pH, pH was gradually decreased than initial stage during pretreatment: of leaf mustard Dolsan at 30, 40 and 50℃. After 16 hours pretreatment, the higher pretreatment temperature was, the higher pH was, but after pretreatment for 48 hours, pH of sample pretreated at 50℃ was lower than that of sample pretreated at 40℃. Antimicrobial activity of leaf mustard Dolsan extract pretreated at 30℃ was the strongest of the samples pretreated at 30, 40 and 50℃, and that of stalk part was stronger than that of leaf part. L and b value in colors was gradually decreased, and a value was increased with the passage of pretreatment time. L and b value was higher in the order of samples pretreated at 40, 50 and 30℃, and the lower pretreatment temperature was, the higher a value was. L value of stalk part was higher than that of leaf part, but a and b value of of leaf part was higher than that of stalk part.

신경섬유종증(렉클링하우젠병)에 의한 급사 1예 보고

이봉우,서중석,권태정,이원태 大韓法醫學會 2000 대한법의학회지 Vol.24 No.1

Neurofibromatosis type1(von Recklinghausen's disease) is characterized by multiple skin tumors and abnormal cutaneous pigmentation(cafe au lait spots). Unlikely the solitary neurofibroma, those encountered in von Recklinghausen's disease may cause significant morbidity. The vasculopathy, although poorly recognized manifestation of von Recklinghausen's neurofibromatosisis a major case of fatal cases. One of its most dramatic presentation is the spontaneous hemothorax. We report a case of Von Recklinghausen's disease with multiple neurofibromatosis accompanying intrathoracic hemorrhage due to vascular rupture, which is the first legal autopsy case in Korea.

성인의 급성 심장사에서 정상 심장례의 분석

강신몽,이원태,이한영,서중석,최영식,권일훈,정낙은 大韓法醫學會 2000 대한법의학회지 Vol.24 No.2

The authors analysed 53 cases of sudden cardiac death(SCD) without prominent structural abnormalies of heart out of witnessed 300 cases. Thirty four cases were associated with sleep and 19 with activity or rest. Twenty one cases were classified as having normal hearts, and the rest as structurally mild abnormal ones. Male was predominant, and almost of the victims were suffered in their twenties or thirties. All cases collapsed instantaneously or in very short time with various and not specific terminal symptoms. Inducing or trigger factors are not clarified in almost of the cases. Mechanisms or causes of death could not be defined clearly. We concluded that careful heart examination with detailed clinical history under the appropriate postmortem investigation system is only effective approach for the true mechanism and cause of death.