Spirometra erinacei의 發育에 따른 Alkaline phosphatase와 Lactate dehydrogenase의 酵素 組織化學的 硏究

朴裁弘,徐淑才,金昌煥 慶尙大學校 기초과학연구소 1990 基礎科學硏究所報 Vol.6 No.-

Spirometra erinacei의 제3기 유충 plerocercoid(Sparganum)를 전이숙주인 흰쥐와 종숙주인 고양이에 감염시켜서 회수한 sparganum과 성충의 alkaline phosphatase와 lactate dehydrogenase(LDH)의 분포 및 동위효소 pattern을 비교하기 위하여 효소조직 화학적 방법과 전기영동법을 이용하여 다음과 같은 실험 결과를 얻었다. 1. Alkaline phosphatase는 sparganum과 성체의 표피층과 표피근층에서 많은 분포를 보였으며 실질근층에도 상당한 분포가 있었다. Isozyme band pattern은 sparganum에서 2개 성체에서 4개가 분리 되었는데, sparganum과 성체 모두 2번 band가 major band였다. 2. LDH는 sparganum의 표피근층과 성체의 표피근층에 많이 분포되었고, sparganum의 실질근층과 표피층에는 거의 분포되지 않았다. LDH의 isozyme band pattern은 sparganum과 성체에서 각각 1개의 band가 분리되었다. This study was performed to investigate distribution and isozyme band pattern of Alkaline phosphatase and Lactate dehydrogenase on developing Spirometra erinacei (sparganum, adult) recovered from sparganum infected rats and cat. The result obtained were as follows ; 1. Alkaline phosphatase had a strong activity in the tegument and the epidermal musculature of sparganum and of adult, but no detectable level in the parenchymal musculature. 2. Two and four Alkaline phospatase isozyme bands were found in sparganum and adult respectively. of these bands, band 2 was major band in sprarganum and adult. 3. The high activity of Lactate dehydrogenase was found in the tegument and epider mal musculature of adult and epidermal musculature of sparganum, but no detctable level in tegumemt and parenchymal musculature of sparganum. 4. Only one lactate dehydrogenase isozyme band was detectable in sparganum and adult.

77K와 상온에서 CO(NH₂)₂의 ¹⁴N Pulsed NQR 연구

강재필,박영민,전인,김성재,최숙자,박현진,송승기,서용문 명지대학교 자연과학연구소 1996 자연과학논문집 Vol.13 No.-

77K와 상온에서 CO(NH₂)₂분말 시료의 ??N Pulsed NQR실험을 하여 위와 아래 공명 진동수, 핵 사중극 결합상수, 비대칭 인자, 스핀-스핀 완화시간, 결정 격자 진동에서의 평균 비틀림 진동수, 진동자의 관성 모먼트 등을 구하였다. 공명 진동수의 온도 의존성은 전형적인 Bayer이론의 격자 진동에 의한 주파수 변화를 보였다. Bayer-Kushida이론의 고온근사식을 실험값에 적용하여 OK에서 공명 진동수 ν??와 ν??를 구했으며, 77K와 상온에서의 스핀-스핀 완화시간 T₂를 관측했다. 상온에서 T₂의 감소는 결정 내의 reorientation motion과 관련이 있을 것으로 보인다. Upper and lower resonance frequencies, nuclear quadrupole coupling constants, asymmetry parameters, spin-spin relaxation time, average torsional frequency in the crystal lattice motion and moment of inertia in the oscillator are found for CO(NH₂)₂powder sample by using ??N Pulsed NQR(Nuclear Quadrupole Resonance) technique at 77K and room temperature. Temperature dependence of resonance frequencies showed frequency variation in agreement with the typical Bayer's theory in lattice motion. Resonance frequencies ν?? and ν?? at OK were obtained by fitting the high temperature approximation of Bayer-Kushida theory to the experimental data and spin-spin relaxation time (T₂) was observed at 77K and room temperature. Decrease of T₂at room temperature was explained by the effect of reorientational motion in the crystal lattice.

녹차 및 결명자 추출물의 교정용 브라켓과 치면 사이의 경계부에서 분리된 mutans streptococci에 대한 항균작용

임성훈,서정순,윤영주,김광원,유소영,김화숙,국중기,이병래,차종희,박재윤 대한치과교정학회 2003 대한치과교정학회지 Vol.33 No.5

본 연구는 치아우식증에 관련된 Mutans streptococci 표준균주 및 임상분리 균주의 성장억제를 유도할 수 있는 천연 생약추출물을 검색하기 위하여 실시되었다. 녹차추출물은 CHMC-2032를 사용하였으며, 결명자 추출물은 50% 에탄올을 이용하여 얻었다. 이들 추출물의 Mutans streptococci 표준균주 및 교정환자들에서 브라켓과 치면 사이의 경계부에서 분리된 각각 10 균주씩의 S. mutans 및 S. sobrinus에 대한 최소성장억제농도를 액체배지 희석법으로 구하였다. 그 결과 CHMC-2032의 S. mutans 및 S. sobrinus의 표준 균주, S. sobrinus의 대부분 임상분리 균주에 대한 최소성장억제농도는 5 mg/ml이었다. 그러나 결명자 추출물에 의한 S. mutans 및 S. sobrinus에 대한 세균 성장억제 효과는 미미하였다. 본 연구 결과 치아우식증의 예방적 측면에서 결명자차보다는 녹차를 마시는 것이 유리하며, CHMC-2032를 이용하여 구강양치용액을 제조하여 사용할 경우 교정환자를 포함하여 대부분의 사람에게서 치아우식증 예방 효과가 있으리고 추정된다. Mutans streptococci is the major causative factor in dental caries. Especially, orthodontic patients with fixed appliance are a risk group for dental caries. Because fixed appliances attached on teeth may change the environment of dental plaque, the enamel decalcification or dental caries around the bracket and band is a major side effect of orthodontic treatment. The aim of this study was to search plant extracts that have antimicrobial effect on mutans streptococci. Seed-extract of Casio tora were prepared with ethanol and CHMC-2032, the leaf-extracts from Camellia sinensis extract, was obtained extract, 2 type strains and 20 clinical isolates of mutans streptococci isolated from the interface between orthodontic brackets and tooth surfaces in the orthodontic patients were used in this study. The minimal inhibitory concentration of CHMC-2032 was 5 mg/ml on the S. mutans KCTC 3065, S. sobrinus KCTC 3088, and 8 clinical isolates of S. sobrinus. However, there was no antibacterial effect of seed-extract of C. tora on mutans streptococci. These data suggest that green tea may be more effective than the tea prepared from C. tora in the prevention of enamel decalcification or dental caries around brackets.

Rhabditis sp.의 생장에 미치는 온도의 영향에 관한 연구

金昌煥,徐淑才 慶尙大學校 1986 論文集 Vol.25 No.2

Rhabditis sp.는 토양선충의 한 종류로 토양내의 유기물을 섭취하는 자유선충인데 광범위한 온도조건에서도 서식하고 있는 것으로 알려져 환경요소 중 온도가 Rhabditis sp.의 개체증식과 생장에 미치는 영향을 검토해 보고자 여러 온도 조건하에서 배양하여 개체의 생장과 증식수 및 대사작용에 관여하는 Esterase와 Acid phosphatase의 pattern 변화를 Electrophoresis를 통하여 조사한 바 다음과 같은 결과를 얻었다. 1. 20℃±4의 온도범위에서 배양된 Rhabditis sp.가 다른 12℃, 32℃ 온도하에서 배양된 Rhabditis sp.와 비교하여 증식수와 체형의 크기에 차이가 있었다. 2. Nanspecific esterase는 총 13개의 Isoenzyme band가 확인되었으며, 각 온도에 따라 12℃는 7개, 16℃·20℃·24℃는 각 6개, 28℃는 5개의 major band를 확인할 수 있었다. band 5번(Rf 0.58)은 전 온도범위에서 확인되었고 band 12번Rf 0.89)는 24℃온도에서만 확인되어 각 band에 따른 활성온도 범위가 각각 달랐다. 3. Acid phosphatase의 Isoenzyme pattern은 총 4개의 Isoenzyme major band 중 band 1. 2. 4. 번은 12℃에서만 major band이었고(Rf 0.14, 0.29, 0.33), band 3번(Rf 0.31)은 16℃ 이상의 온도에서 major band이었다. The present studies was carried out to investigate the influence of temperature on the rate of growth and on the multiplication of Rhabditis sp.. Rhabditis sp. was cultured at various temperature levels (12, 20, 24, and 28℃) and analysed by electrophoresis to investigated the isozyme pattern of nonspecific esterase and acid phosphatase. The results obtained were as follows. 1. Rhabditis sp. cultured at 20℃±4 showed differences in the rate of multiplication and body lengths, as compared with that at 12° or 32℃. 2. A total of 13 isoenzyme bands of nonspecific esterase was idendified and the numbers of major bands at esch temperature(12, 16, 20, 14 and 28℃) were 7, 6, 6, 6, and 5, respectively. Band 5 appeared at all temperatures tested, whereas band 12 appeared only at 24℃. 3. The patterns of acid phosphatase isoeozymes showed 4 major bands.. Out of 4 major bands band 1, 2 and 4 represented as major bands only at 12℃, whereas band 3 represented as major band at temperatures above 16℃. The results showed that the temperatures had effect on the activity of isoenzyme bands.

흰쥐에 있어서 Sparganum의 移行과 發育에 관한 硏究

金昌煥,徐淑才,崔朱卿 慶尙大學校 1985 論文集 Vol.24 No.1

This study was carried out to investigate the migration and development of the Sparganum in rats and the changes of haematological values in the hosts infected with Sparganum. The results obtained were summarized as follows: 1. The recovery rate of the Sparganum in the infected rats was 57 per cent on the autopical findings of the infected individual rats during observations from 2 hours to 32 days after infection with Sparganum. 2. The larval Sparganum was found in the digestive tract within two hours after administration with oral route and then, the worms began to penetrate and migrate via the abdominal cavity within 4 to 6 hours and the worms were found in the muscular tissues 2 days onwards after infections. 3. The infected Sparganum began to deveolp the size of the body from 4 day after infection and then, the size of the Sparganum was measured by 52.9mm long and 3.2mm broad on 32 days after infection . 4. In the haematological observation of the host the red blood cell values changed little, while the white blood cells were increased significantly during 2 days after infection and thereafter they were recovered to the normal values.

퍼지 추론을 이용한 분류 벡터 양자화기의 성능 개선

황병하,김혜숙,서재성,박민식,곽훈성 全北大學校 1994 論文集 Vol.37 No.-

In Classified Vector Quantization(CVQ) for image coding, the block classification pondering the block characteristics will be the most important procedure if we consider system performance. But it does not take effect because classification of conventional CVQ followed act on a heuristic and statistics method. In order to solve the problem of conventional methods, i.e, for block classification effectively we propose a fuzzy inferencing algorithm to improve its performance. To do this, fuzzy rules must be generated in the first. Our rules are based on energy distribution in transform domain with respect to spatial variation. Ultimately fuzzy inferencing of this paper apply these fuzzy rules for efficient block classification on a input image. As a result of efficient classification, our proposed method shows that blocking effect is decreased and PSNR(peak to peak signal to noise ratio) is increased by about 1 ∼ 2 dB for 0.2 ∼ 0.6 bpp range, when the performance is compared with Aizawa's scheme^[9].

암 병동 간호사의 통증관리에 대한 지식과 태도

공성화,방찌야,서민숙,현신숙,김희정,이미애1,이미애2,유현희,허재경,김은애,박경숙 성인간호학회 2004 성인간호학회지 Vol.16 No.1

Purpose: The purpose of this study is to evaluate the knowledge and attitudes of oncology ward nurses toward cancer pain management and to find ways to improve the educational program for nurses. Method: A total of 209 nurses working at the oncology ward of three hospitals in Seoul and a Gyenggi Province. The survey instrument used was the 32-item scale for evaluating nurses knowledge and attitudes originally developed by McCaffery and Ferrell'(1990). that was by Kim'(1997). Result: In terms of the nurses knowledge of pain management. the result showed that the nurses scored an average of 67.8 out of 100 for phamacokinetics of opioids. 84.8 for classification of analgesics, 60.1 for pain assessment, and 70.7 for drug administration. 18.2% of the nurses hesitate to inject the narcotic agent because of concerns regarding the drug's potential side effects. there was significant difference in the knowledge of pain management according to the general characteristics of pain in terms of the nurses age(p=.001), position (p=.016). years of experiences(p=.002), experience of cancer pain education(p= .001). Conclusion: The also showed that nurses working at cancer ward lack knowledge. It is important to provide intensive education to nurses about cancer pain management.

갑상선 유두암이 병발된 가족성 대장 용종증 1예

백종현,소창배,이은영,이지숙,신은경,박은호,서정아,양재홍,송준영,박선자,박요한,최영식 고신대학교의과대학 2007 고신대학교 의과대학 학술지 Vol.22 No.2

Familial adenomatous polyposis (FAP) is an autosomal dominant syndrome, typically characterized by multiple colorectal adenomas and increased incidence of colorectal carcinomas if it is not treated. Moreover, a variety of extracolonic manifestation are seen. The prevalence of thyroid tumors developing in patients with FAP is about 1∼2%. Generally, papillary thyroid cancer indicates low mortality but ,however, as people get older, the cancer becomes more aggressive. So, the operation is required. Recently we experienced a case of FAP, presenting with papillary thyroid carcinoma, and reported with a brief review of literatures.

중량물 운반시 잡기유형과 부하단계에 따른 생리학적 연구

윤양진,이훈식,윤길환,정미라,이재규,이중숙,서국웅 釜山大學校 附設 體育科學硏究所 1999 體育科學硏究所 論文集 Vol.15 No.-

The purpose of this study was to examine physiological changes of the body according to various weight loads and the holding during carrying object. The experiments were conducted with utilizing physiological analyses for five male and five female adults as subjects. The results were as followed. 1. Any significant difference was not shown in heart rate, ventilation and amount of oxygen uptake of male subjects in the case of no load and 5kg. And also no significant difference was shown in expiratory exchange ratio of male subjects of no load, 5kg and 10kg, but in other measure items and other stages of weight was shown. 2. Significant differences were shown in heart rate, ventilation, defluxion of carbon dioxide, oxygen uptake per body weights, respiratory exchange rate, respiration rate and all other measure items of female subjects in all stages of load. 3. In the MMH job, there was no influence on male subjects up to 5kg loads, but there was an significant difference in male subjects in load walking from 5kg. It shows that even in 5kg weight MMH work, female subject's expiratory function was influenced. 4. 10kg load was the limit weight of female subjects and 15kg was of male subjects when they walked on treadmills with 4km/h velocity for 3 minutes, holding loads in the right and vertical types.

Phase II study of vinorelbine monotherapy in anthracycline and taxane pre-treated metastatic breast cancer

Seo, Hee Yeon,Lee, Hyun Joo,Woo, Ok Hee,Park, Kyong Hwa,Woo, Sang Uk,Yang, Dae Sik,Kim, Ae-Ree,Lee, Jae-Bok,Lee, Eun Sook,Kim, Yeul Hong,Kim, Jun Suk,Seo, Jae Hong Springer-Verlag 2011 Investigational new drugs Vol.29 No.2