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- 저자 : Selvarasu, Suresh (검색결과 4 건)
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Combined in silico modeling and metabolomics analysis to characterize fed‐batch CHO cell culture
Selvarasu, Suresh,Ho, Ying Swan,Chong, William P. K.,Wong, Niki S. C.,Yusufi, Faraaz N. K.,Lee, Yih Yean,Yap, Miranda G. S.,Lee, Dong‐,Yup Wiley Subscription Services, Inc., A Wiley Company 2012 Biotechnology and bioengineering Vol.109 No.6
<P><B>Abstract</B></P><P>The increasing demand for recombinant therapeutic proteins highlights the need to constantly improve the efficiency and yield of these biopharmaceutical products from mammalian cells, which is fully achievable only through proper understanding of cellular functioning. Towards this end, the current study exploited a combined metabolomics and in silico modeling approach to gain a deeper insight into the cellular mechanisms of Chinese hamster ovary (CHO) fed‐batch cultures. Initially, extracellular and intracellular metabolite profiling analysis shortlisted key metabolites associated with cell growth limitation within the energy, glutathione, and glycerophospholipid pathways that have distinct changes at the exponential‐stationary transition phase of the cultures. In addition, biomass compositional analysis newly revealed different amino acid content in the CHO cells from other mammalian cells, indicating the significance of accurate protein composition data in metabolite balancing across required nutrient assimilation, metabolic utilization, and cell growth. Subsequent in silico modeling of CHO cells characterized internal metabolic behaviors attaining physiological changes during growth and non‐growth phases, thereby allowing us to explore relevant pathways to growth limitation and identify major growth‐limiting factors including the oxidative stress and depletion of lipid metabolites. Such key information on growth‐related mechanisms derived from the current approach can potentially guide the development of new strategies to enhance CHO culture performance. Biotechnol. Bioeng. 2012; 109:1415–1429. © 2012 Wiley Periodicals, Inc.</P>
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Selvarasu Praneetha,Yun-Sung Lee,Vanchiappan Aravindan 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.112 No.-
Vanadium pentoxide (V2O5) brings vast interest in the promising host materials for the intercalation ofmultivalent ions, owing to its abundance in the earth crust, synthesizing facile methodologies, and offersmaximum discharge capacity of >300 mAh g1. However, V2O5 undergoes different phase transformationsupon the intake of beyond 1 mol Li. Here, we report a comparative study of two versatile cathodematerials, such as V2O5 (limiting 1 mol. Li) and LiFePO4. A solvothermal method is adopted to synthesizeboth two, and three-dimensional crystalline phases of V2O5 and LiFePO4, respectively. The sphericalshapedV2O5 exhibits the initial discharge capacity of 136 mAh g1 in the half-cell assembly and rendersstable cycle life. Subsequently, V2O5 is paired with the electrochemically lithiated graphite (LiC6) anode infull-cell assembly (V2O5/LiC6) and offers a maximum energy density of 266.7 Wh kg1 (based on totalmass loading). On the other hand, LiFePO4 also exhibits 136 mAh g1 in the half-cell performance withstable cycle life. The full-cell LiFePO4/C delivers an energy density of 234.8 Wh kg1. This clearly encouragesthat V2O5 is a strong contender for the 3.4 V class Li-ion cells and paves the new avenue for furtherexploration of advanced battery technologies.
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Selvarasu, Suresh,Ow, Dave Siak-Wei,Lee, Sang Yup,Lee, May May,Oh, Steve Kah-Weng,Karimi, Iftekhar A.,Lee, Dong-Yup Wiley Subscription Services, Inc., A Wiley Company 2009 Biotechnology and Bioengineering Vol.102 No.3
<P>Genome-scale flux analysis of Escherichia coli DH5α growth in a complex medium was performed to investigate the relationship between the uptake of various nutrients and their metabolic outcomes. During the exponential growth phase, we observed a sequential consumption order of serine, aspartate and glutamate in the complex medium as well as the complete consumption of key carbohydrate nutrients, glucose and trehalose. Based on the consumption and production rates of the measured metabolites, constraints-based flux analysis of a genome-scale E. coli model was then conducted to elucidate their utilization in the metabolism. The in silico analysis revealed that the cell exploited biosynthetic precursors taken up directly from the complex medium, through growth-related anabolic pathways. This suggests that the cell could be functioning in an energetically more efficient manner by reducing the energy needed to produce amino acids. The in silico simulation also allowed us to explain the observed rapid consumption of serine: excessively consumed external serine from the complex medium was mainly converted into pyruvate and glycine, which in turn, led to the acetate accumulation. The present work demonstrates the application of an in silico modeling approach to characterizing microbial metabolism under complex medium condition. This work further illustrates the use of in silico genome-scale analysis for developing better strategies related to improving microbial growth and enhancing the productivity of desirable metabolites. Biotechnol. Bioeng. 2009; 102: 923–934. © 2008 Wiley Periodicals, Inc.</P>
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Design and optimization of turbonator using blade element theory
K. Selvarasu,R. Mohan 한양대학교 청정에너지연구소 2023 Journal of Ceramic Processing Research Vol.24 No.3
Due to the quick increase in automobile usage, there is currently an ecological imbalance brought on by air pollution that isgetting worse by the hour. Car exhaust gas emissions must be reduced, and better fuel combustion is a necessity. Despite theshort amount of time needed for combustion, no fuel is burned, and all of the fuel returns as exhaust. In order to prevent this,the burning of diesel engines results in a sufficient mass of air in the air-fuel mixture (lean mixture), which leads to cheap fuelintake and greater volumetric efficiency. A supercharger, more specifically a turbocharger, increases the diesel engine'svolumetric efficiency. However, installing a turbocharger in a vehicle engine demands extra room, size, and maintenance. Asa result, a Turbonator is added to the diesel engine's air intake manifold, creating swirl in the cylinder-air flow. Theenhancement of IC engine efficiency has been conceded by the reduction of fuel consumption and emission levels. Manyresearchers worked in the alternate fuel system, design modification in the intake manifold, piston, engine cylinder,turbocharger system, etc. The turbocharger system helps to reduce the emission level of diesel engines in current trends. Inthis research, emission reduction and engine performance are improving by introducing turbonator fitted into the intakemanifold. The design of turbonator is designed by Blade Element Theory (BET), which generates a swirl in the flow of airinto the cylinder. The number of blades in turbonator has varied as 4, 8, & 12 respectively for a better outcome. Theexperimental load test is carried out in the KIRLOSKAR TV-I engine for 10 cc of fuel consumption to prove the turbonatorperformance. The turbonator with 4 blades gives a better performance than it suitable to implement into the diesel engineintake.
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