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Govarthanan, Muthusamy,Selvankumar, Thangasamy,Manoharan, Koildhasan,Rathika, Rajiniganth,Shanthi, Kuppusamy,Lee, Kui-Jae,Cho, Min,Kamala-Kannan, Seralathan,Oh, Byung-Taek Dove Medical Press 2014 INTERNATIONAL JOURNAL OF NANOMEDICINE Vol.9 No.-
<P>Synthesis of silver nanoparticles (AgNPs) with biological properties is of vast significance in the development of scientifically valuable products. In the present study, we describe simple, unprecedented, nontoxic, eco-friendly, green synthesis of AgNPs using an Indian traditional farming formulating agent, panchakavya. Silver nitrate (1 mM) solution was mixed with panchakavya filtrate for the synthesis of AgNPs. The nanometallic dispersion was characterized by surface plasmon absorbance measuring 430 nm. Transmission electron microscopy showed the morphology and size of the AgNPs. Scanning electron microscopy–energy-dispersive spectroscopy and X-ray diffraction analysis confirmed the presence of AgNPs. Fourier transform infrared spectroscopy analysis revealed that proteins in the panchakavya were involved in the reduction and capping of AgNPs. In addition, we studied the antibacterial activity of synthesized AgNPs. The synthesized AgNPs (1–4 mM) extensively reduced the growth rate of antibiotic resistant bacteria such as <I>Aeromonas</I> sp., <I>Acinetobacter</I> sp., and <I>Citrobacter</I> sp., according to the increasing concentration of AgNPs.</P>
Muthusamy Govarthanan,카말라칸,Thangasamy Selvankumar,Kandasamy Selvam,Chinnappan Sudhakar,Vincent Aroulmoji 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.27 No.-
A native feather-degrading keratinolytic bacterium, Bacillus sp. MG-MASC-BT was isolated from featherdumping soil in Mallasamudram, Tamil Nadu, India and screened for keratinase production using alkalitreated horn waste (HW) and feather waste (FW). The study factors influencing keratinase productionwas optimized by Box–Behnken design (BBD). The maximum enzyme production (1075 U/ml) wasobserved at pH 7.0, temperature 55 8C and growth period of 60 h. The media supplemented with 6% ofHW and FW enhanced keratinase production. Statistics based contour plots were generated to evaluatethe changes in the response surface and to understand the relationship between the enzyme yield andthe culture conditions.
M. Govarthanan,R. Mythili,T. Selvankumar,카말라칸,최두복,장영철 한국생물공학회 2017 Biotechnology and Bioprocess Engineering Vol.22 No.2
Objective of the study was to isolate heavy metal resistant bacteria from chromium-contaminated subsurface soil and investigate biosurfactant production and heavy metal bioremediation. Based on 16S rRNA gene sequence and phylogenetic analysis, the isolate was identified as Rahnella sp. RM. The biosurfactant production by heavy metal resistant Rahnella sp. RM was optimized using Box- Behnken design (BBD). The maximum emulsification activity was obtained 66% at 6% soybean meal in pH 7.0 and 33.5°C. The biosurfactant was characterized using Field emission scanning electron microscopy (FE-SEM), Fourier transform infrared spectroscopy (FT-IR) and matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF). The highest metal removal rates using the biosurfactant were found 74.3, 72.5, and 70.1%, respectively, at the 100 mg/L amended flasks at 48 h. This study indicated the biosurfactant from heavy metal resistant Rahnella sp. RM could be used as a potential tool to remediate the metals in contaminated environments.
Aravinthan, Adithan,Govarthanan, Muthusamy,Selvam, Kandasamy,Praburaman, Loganathan,Selvankumar, Thangasamy,Balamurugan, Rangachari,Kamala-Kannan, Seralathan,Kim, Jong-Hoon Dove Medical Press 2015 INTERNATIONAL JOURNAL OF NANOMEDICINE Vol.10 No.-
<P>A rapid, green phytosynthesis of silver nanoparticles (AgNPs) using the aqueous extract of <I>Helianthus tuberosus</I> (sunroot tuber) was reported in this study. The morphology of the AgNPs was determined by transmission electron microscopy (TEM). Scanning electron microscopy–energy-dispersive spectroscopy (SEM–EDS) and X-ray powder diffraction (XRD) analysis confirmed the presence of AgNPs. Fourier transform infrared spectroscopy (FTIR) analysis revealed that biomolecules in the tuber extract were involved in the reduction and capping of AgNPs. The energy-dispersive spectroscopy (EDS) analysis of the AgNPs, using an energy range of 2–4 keV, confirmed the presence of elemental silver without any contamination. Further, the synthesized AgNPs were evaluated against phytopathogens such as <I>Ralstonia solanacearum</I> and <I>Xanthomonas axonopodis</I>. The AgNPs (1–4 mM) extensively reduced the growth rate of the phytopathogens. In addition, the cytotoxic effect of the synthesized AgNPs was analyzed using rat splenocytes. The cell viability was decreased according to the increasing concentration of AgNPs and 67% of cell death was observed at 100 μg/mL.</P>