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RISS 인기검색어
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- 저자 : Sean McDonough (검색결과 2 건)
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Yung-Fu Chang,Sung Jae Shin,유한상,Sean P. McDonough 대한수의학회 2004 Journal of Veterinary Science Vol.5 No.2
Eighty-five complex (85A, 85B and 85C), 35-kDa and superoxide dismutase (SOD) were cloned, expressed and purified as antigens in an enzyme-linked immunosorbent assay (ELISA) to compare the serological reactivity of cows with different shedding levels of Mycobacterium avium subsp. paratuberculosis (MPT). Antibody responses to all recombinant antigens positively increased depending on shedding levels. In particular, antibody responses to the 35 kDa were higher than those to the others in all shedder groups. Also, the mean of O. D. values among Ag 85 complex, 85B showed slightly higher response than others with high sensitivity and specificity in all shedder groups. In receiver operating characteristic (ROC) curve analysis, the result of 35 kDa ELISA yielded an area under the curve value of 0.945 (95% confidence interval = 0.895 − 0.996), which indicated that this 35 kDa is more accurate indicator of MPT infection than other antigens. At the cut-off point recommended by the ROC curve analysis, the sensitivity and specificity of 35 kDa ELISA were higher than those of other antigens with 93.3% and 86.4%, respectively. Finally, a commercially available ELISA kit was used to clarify 200 positive and 200 negative sera. We then re-tested these serum samples with our ELISA test using the 35-kDa antigens. 35 kDa ELISA and commercial kit showed almost similar results in ROC curve analysis even though two of positive sera in commercial kit were negative in 35 kDa ELISA. The sera, which showed difference in the comparison with commercial ELISA kit, they also did not react with 35 kDa in Western blot. These results suggest that a 35-kDa based ELISA can be useful for detecting MPT infection. Eighty-five complex (85A, 85B and 85C), 35-kDa and superoxide dismutase (SOD) were cloned, expressed and purified as antigens in an enzyme-linked immunosorbent assay (ELISA) to compare the serological reactivity of cows with different shedding levels of Mycobacterium avium subsp. paratuberculosis (MPT). Antibody responses to all recombinant antigens positively increased depending on shedding levels. In particular, antibody responses to the 35 kDa were higher than those to the others in all shedder groups. Also, the mean of O. D. values among Ag 85 complex, 85B showed slightly higher response than others with high sensitivity and specificity in all shedder groups. In receiver operating characteristic (ROC) curve analysis, the result of 35 kDa ELISA yielded an area under the curve value of 0.945 (95% confidence interval = 0.895 − 0.996), which indicated that this 35 kDa is more accurate indicator of MPT infection than other antigens. At the cut-off point recommended by the ROC curve analysis, the sensitivity and specificity of 35 kDa ELISA were higher than those of other antigens with 93.3% and 86.4%, respectively. Finally, a commercially available ELISA kit was used to clarify 200 positive and 200 negative sera. We then re-tested these serum samples with our ELISA test using the 35-kDa antigens. 35 kDa ELISA and commercial kit showed almost similar results in ROC curve analysis even though two of positive sera in commercial kit were negative in 35 kDa ELISA. The sera, which showed difference in the comparison with commercial ELISA kit, they also did not react with 35 kDa in Western blot. These results suggest that a 35-kDa based ELISA can be useful for detecting MPT infection.
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Equine hyperimmune serum protects mice against Clostridium difficile spore challenge
Weiwei Yan,Kang-Soon Shin,Shih-Jon Wang,Hua Xiang,Thomas Divers,Sean McDonough,James Bowman,Anne Rowlands,Bruce Akey,Hussni Mohamed,Yung-Fu Chang 대한수의학회 2014 Journal of Veterinary Science Vol.15 No.2
Clostridium (C.) difficile is a common cause of nosocomialdiarrhea in horses. Vancomycin and metronidazole havebeen used as standard treatments but are only moderatelyeffective, which highlights the need for a novel alternativetherapy. In the current study, we prepared antiserum ofequine origin against both C. difficile toxins A and B as wellas whole-cell bacteria. The toxin-neutralizing activities of theantibodies were evaluated in vitro and the prophylacticeffects of in vivo passive immunotherapy were demonstratedusing a conventional mouse model. The data demonstratedthat immunized horses generated antibodies against bothtoxins A and B that possessed toxin-neutralizing activity. Additionally, mice treated with the antiserum lost less weightwithout any sign of illness and regained weight back to anormal range more rapidly compared to the control groupwhen challenged orally with 107 C. difficile spores 1 day afterserum injection. These results indicate that intravenousdelivery of hyperimmune serum can protect animals from C. difficile challenge in a dose-dependent manner. Hence,immunotherapy may be a promising prophylactic strategyfor preventing C. difficile infection in horses.
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