Induction of heme oxygenase-1 protects against podocyte apoptosis under diabetic conditions

Lee, Sang Choel,Han, Seung Hyeok,Li, Jin Ji,Lee, Sun Ha,Jung, Dong-Sub,Kwak, Seung-Jae,Kim, Seung Hye,Kim, Dong Ki,Yoo, Tae-Hyun,Kim, Jin Hyun,Chang, Se-Ho,Han, Dae Suk,Kang, Shin-Wook International Society of Nephrology 2009 Kidney international Vol.76 No.8

Heme oxygenase-1 (HO-1) is an anti-oxidant enzyme normally upregulated in response to oxidant injury. Here we determined the role of HO-1 in podocyte apoptosis in glomeruli of streptozotocin-treated rats and in immortalized mouse podocytes cultured in media containing normal or high glucose. HO-1 expression, its activity, the ratio of Bax/Bcl-2 protein, and active caspase-3 fragments were all significantly higher in isolated glomeruli of diabetic rats and in high glucose–treated podocytes. These increases were inhibited by zinc protoporphyrin treatment of the rats or by HO-1 siRNA treatment of the podocytes in culture. The number of apoptotic cells was also significantly increased in the glomeruli of diabetic rats and in high glucose–treated podocytes. Inhibition of HO-1 accentuated the increase in apoptotic cells both in vivo and in vitro. Our findings suggest that HO-1 expression protects against podocyte apoptosis under diabetic conditions.

나지(裸地)에서 벌개미취 생장에 미치는 재식거리의 영향

신세균,박재호,전종옥,윤태,윤종선 韓國植物ㆍ人間ㆍ環境學會 2001 인간식물환경학회지 Vol.4 No.3

The Primary goal of this research is to verify the usefulness of Aster Koraiensis as a garden plant and to figure out the appropriate planting space which would bring the most effective results in terms of scenery and cost. For these purpose, we gathered the saplings of the wild grown Aster koraiensis in August and maintained them for winter. The following spring, we planted them in flat bare land with 3 different panting spaces of 50 x 50Cm, 40x30Cm, 30x30Cm. 2nd years after planting, we observed and compared the 3 different columns on the status of growth, number and size of leaves and conditions of flowering. The results are as follows : The number of leaves, stalks and roots of individual plants were increased by the wider planting density. But the ratio of leaf area per unit area appeared to be the highest in the 40X30cm sector which showed 366.9%, while the 50x50cm column showed 294.1% and 360.4% in the 30X30cm column. As for the conditions of flowering, such as the size of flowers, the number of petals per blossom and the length of flowerstalks, the best results were observed in the 50x50cm column. On the other hand, the 40x30cm column yielded the largest number of blossoms per unit area. On the basis of these results, we could induce that the 30x30cm column had already passed the peak point for the best scenery within 2 years after planting, while the 50x50cm column had not yet reached the peak point. Naturally, this leads to the conclusion that the 40x40cm planting space could be most effective when we assume the peak period for scenery to be 2nd years after planting. According to our investigation, the blooming period of Aster Koraiensis in the central region of the Korean peninsular lasts about 70 days from 15th June to 25th August. And the peak blooming time is 30 days from 5th July to 5th August. On the basis of these findings, we can reach the final conclusion that Aster Koraiensis has many merits and is useful as ornamental flavor of garden.

2급 와동 수복 시 한국 치과 의사들의 복합레진 사용 실태 연구

신동호,박세은,양인석,장주혜,이인복,조병훈,손호현 대한치과보존학회 2009 Restorative Dentistry & Endodontics Vol.34 No.2

본 연구는 2008년 1월 현재 한국 치과의사들의 복합레진 사용 실태에 관한 설문 조사로서, 2급 와동을 수복하는 증례에서 치과의사의 면허 년도, 교육 배경 및 진료환경 등에 따라 수복재료의 선택, 복합레진의 사용여부 및 사용방법, 문제점 등을 비교하였다. 한국 치과의사들을 대상으로 2급 와동 수복에 관한 17 문항의 설문지를 제작하였다. 이 설문지는 시술자 정보 2급 와동의 수복으로 크게 2부분으로 나누어진다. 이 설문지는 대한치과의사협회를 통해 12.193 명의 치과의사들에게 E-메일로 발송되었다. 이 중 2.612개의 메일이 수신 확인되었고 840 개의 설문지가 작성되어 회신되었다. 수신 확인된 메일 수에 대한 회신된 메일 수의 비율 (회신율)은 32.2%이었고, 이 자료는 SPSS프로그램에서 카이제곱 분석을 이용하여 교차 분석하였다. 답변자의 비율은 면허년도를 기준으로 1998 ~ 2007년에 면허를 취득한 그룹 (한국 전체 치과의사의 33 3%, 추정 년령 26-35세)이 60.3%로 1997년 이전에 면허 취득한 그룹 (한국 전체 치과의사의 66.7%.추정 년령 36세 이상)의 39.7%보다 높은 것으로 나타났다. 또한 이들이 근무하는 병원은 개인의원 (77%)이 가장 많았고 남자 치과의사(79%)가 많았다. 복합레진 수복에 대한 지식은 학생 때 수업이나 학회, 세미나를 통하여 (83.4%) 얻은 것으로 나타났다. 2급 와동 수복 시 재료 선호도를 살펴보면 금인레이가 65.7%를 차지하고 있고 복합레진 직접 수복은 12.1%로 낮게 나타났다. 2급 와동의 복합레진 직접 수복 시 시술 방법에 있어서는 러버댐을 사용하지 않거나 잘 사용하지 않는 그룹의 비율(74.4%)이 더 많았고. 격벽법으로는 mylar strip (53.4%)이나 metal matrix (33.8%), Palodent svstem (6.5%)를 사용하였다. 충전은 적층법 (99.6%)을 사용하는 것으로 나타났으며, 시술 시 인접면 형성을 가장 어려워하였다.(57.2%). 2-step 접착시스템 (76%)이 3-step 접착시스템 (16%)보다 많이 사용되고 있었다. 사용하는 레진 제품으로는 Z250(20%)이 가장 많이 사용되고 있었다. The purpose of this study was to assess the current materials, methods and difficulties according to the year of licence and educational background of Korean dentists in Class Ⅱ direct composite resin restorations. Total 17 questions were included in the questionnaire. Questions were broadly divided into two parts first, operator's information and second the materials and methods used in Class Ⅱ posterior composite restoration. The questionnaire was sent to dentists enrolled in Korean Dental Association via e-mail. Total 12,193 e-mails were distributed to dentists. 2,612 e-mails were opened and 840 mails (32.2%) were received from respondents. The data was statically analyzed by chi-square test using SPSS(v 120.1 SPSS Inc. Chicago. IL USA). Male dentists among respondents was 79% 60.3% of the respondents acquired their licences recently (1998-2007) and 77% practiced in private offices 83.4% have acquired their knowledge through school lectures conferences and seminars. For the Class Ⅱ restorations gold inlays were preferred by 65.7% of respondents while direct composite resin restorations were used by 12.1% amalgam users were only 4.4% of respondents. For the restorative technique 74.4% of respondents didn't use rubber dam as needed. For the matrix. mylar strip (53.4%), metal matrix (33.8%) and Palodent system (6.5%) were used 99.6% of respondents restored the Class Ⅱ cavity by incremental layering. Obtaining of the tight interproximal contact was considered as the most difficult procedure (57.2%) followed by field isolation (21%). Among various bonding systems, 22. 6% of respondents preferred SE Bond and 20.2% used Single Bond. Z-250 was used most frequently among a variety of composite resins.

Prazosin의 항고혈압 효과에 대한 임상적 관찰

서세웅,이균태,김연호,조성락,신영우,신영기 최신의학사 1984 最新醫學 Vol.27 No.8

경막 천자 후 두통 치료 목적의 자가 혈액 봉합술 시행 중 발생한 기뇌증

정순호,김책,김영환,임세훈,이정한,이근무,최영균,김영재,신치만 인제대학교 2006 仁濟醫學 Vol.27 No.-

A 57-year-old woman who was suffered from chronic back pain and radiating pain to left leg visited the local pain clinic. An accidental dural puncture happened at the L4-5 intervertebral space during the procedure with the loss of resistance technique using air. The epidural needle was removed, and a new insertion was done at the L3-4 intervertebral space with administration of the 0.5% lidocaine 8 ml mixed triamcinolone 40 mg. On the next day, she visited again the local pain clinic with complaining of headache. During an epidural autologuous blood patch procedure, she complained of a severe headache, nausea, vomiting, and developed generalized tremor of abrupt onset. There was no evidence of a dural puncture during the procedure. She was referred to our hospital at once. A brain CT scan showed air-filled cavities in basal cistern. She was given conservative treatment during 14 days. After 10 days, follow up brain CT scan showed no air-filled cavity in basal cistern. She was discharged from our hospital without complications.

대장직장종양에 있어서 Hand-Assisted Laparoscopic Surgery (HALS)의 초기 경험

조호영,최선근,구지회,허윤석,이건영,김세중,조영업,안승익,홍기천,신석환,김경래,우제홍 대한내시경복강경외과학회 2003 대한내시경복강경외과학회지 Vol.6 No.-

흰쥐 갑상선 소포세포의 분화에 관한 전자현미경적 연구

황세진,정호삼,유신철,이규식 한양대학교 의과대학 1991 한양의대 학술지 Vol.11 No.1

The thyroid gland is an endocrine organ which is mainly derived from endodermal cell of the primitive pharynx. It is well known that time of morphological and functional maturation of thyroid gland is so variable among the species of animal that functionl maturation of thyroid gland of the rat occurs only after birth of the animal, while the human fetal thyroid gland can secrete thyroid hormones at 10-14 weeks of gestation. In this study the author pursued the ultrastructural differentiation of thyroid follicular cells of rats in the late fetal and early postnatal lives. The fetal rats of 18th and the 20th day of gestation and rats of the 1st, the 7th, the 14th and the 21st day of postnatal life were sacrificed and thyroid glands were extracted. Rats of the 8th weeks of postnatal life were served as control group. All specimens prefixed in 2% glutaraldehyde-2.5% paraformaldehyde and postfixed in osmium tetraoxide, were dehydrated and embeded in Epon Araldite 502. Ultrathin section about 600-800 in thickness were made with LKB ultramicrotome and stained with uranyl acetate and lead citrate and lead citrate All electron microscopic preparations were observed with JEM 100cx-II electron microscope The result obtained were as follows: 1. The nucleus of the follicular cell in the rat thyroid gland was composed of euchromatin at the 18th day of gestation, some unclei with euchromatin and heterochromatin were observed at the 20th day of gestation, and all nuclei of the follicular cells were composed of euchromatin and heterochromatin after the 1st day of postnatal life. 2. Rough endoplasmic reticulum of the follicular cell in the rat thyroid gland was consisted of column shaped scant cisternae at the 18th day of gestation, and rough endoplasmic reticulum with unmerous dilated cisterne was observed at the 7th day of gestation. 3. The Golgi complex of the follicular cell in the rat thyroid gland was composed of narrow cisternae and small sized vesicles at the 18th day of gestation. Folgi complex with wide cisternae and large sized vesicles and vacuoles was observed at the 14th day of posnatal life. 4. Mitochondria of the follicular cell in the rat thyroid gland were scanty at the 18th day of gestation and number of mitochondria increased during development of thyroid gland. 5. Lysosmes of the follicular cell in the rat thyroid gland were observed from the 1st day of postnatal life. 6. Collid lumen and microvilli of the thyroid follicle were observed from the 20th day of gestation and the matured colloid lumen was observed at the 1st day of postnated life. It is consequently suggested that the thyroid follicleis are formed from the 20th day of gestation to the 1st day of postnatal life, and cytoplasmic organelles of the folliculat cell are matured morphologically between the 7th and the 14th day of postnatal life in albino rats.

전기경련요법에서 remifentanil이 혈역학에 미치는 효과

정순호,이상은,임세훈,이정한,이근무,최영균,김영재,신치만 白中央醫療院 2005 仁濟醫學 Vol.26 No.1

Objective: Electroconvulsive therapy (ECT) is often associated with transient hypertension and tachycardia. Acute hemodynamic change may produce serious complications. Remifentanil is a potent ultra short acting opioid and has been found to reduce blood pressure and heart rate. The goal of this study was to evaluate effects of remifentanil on seizure duration, recovery parameters and hemodynamic changes during ECT. Methods: Ten ASA Ⅰ, Ⅱ patients enrolled in this prospective, randomized trial, each receiving more than four electroconvulsive therapy. Patients were randomized to receive placebo, group P (propofol 1mg/kg+0.9% normal saline 1ml, n=10), and propofol with remifentanil, group R (propofol 1mg/kg+remifentanil 1ug/Kg n=10). Supplemental dose of propofol was given as required to achieve loss of consciousness. We recorded hemodynamic parameters, seizure duration, and recovery parameters. Results: Seizure duration and recovery parameters were similar in group R and group P. Blood pressure and Hear rate after ECT were significantly lower in group R. Conclusions: Remifentanil(1ug/kg) attenuated the acute hemodynamic response to ECT under propofol anesthesia without adversely affecting the duration of seizure activity and recovery profile. Adjunctive use of remifentanil may be good alternative regimens for ECT.

집적 결상계의 기본 영상을 이용한 연속 시점 이차원 영상의 합성

박세희,장재영,서장일,차성도,신승호 강원대학교 정보통신연구소 2004 정보통신논문지 Vol.8 No.-

In this paper, we have studied continuous parallax two-dimensional(2D) image composition by use of elemental images which are picked up in integral imaging system. The 2D image composition method has been analyzed in terms of geometrical optics, and extended to the orthoscopic reconstruction. From the proposed composition method, it has been shown that the 2D image can be composed with arbitrary perspective and focus.

스코폴라민부착포에 의해 발생한 중추성항콜린 증후군 1예

신동성,이호선,이세진 영남대학교 의과대학 2012 Yeungnam University Journal of Medicine Vol.29 No.1

Central anticholinergic syndrome occurs when an anticholinergic substance works in vivo or as a result of the insufficient release of acetylcholine. Its symptoms include confusion, agitation, behavioral change, hallucination, blurred vision, and dysarthria. Occasionally, these symptoms occur with the use of a scopola- mine patch. A 54-year-old female complained of behavioral change and confused mentality. She attached a scopolamine patch at the postauricular area in the morning of the day before her hospital visit. Neurolo- gical examination revealed bilateral symmetric mydriasis without light reflex. The brain MRI was normal, and electroencephalography showed nonspecific abnormalities. The patient recovered completely after the removal of the scopolamine patch.