Bucillamine prevents cisplatin-induced ototoxicity through induction of glutathione and antioxidant genes

Kim, Se-Jin,Ho Hur, Joon,Park, Channy,Kim, Hyung-Jin,Oh, Gi-Su,Lee, Joon No,Yoo, Su-Jin,Choe, Seong-Kyu,So, Hong-Seob,Lim, David J,Moon, Sung K,Park, Raekil Nature Publishing Group 2015 Experimental and molecular medicine Vol.47 No.2

<P>Bucillamine is used for the treatment of rheumatoid arthritis. This study investigated the protective effects of bucillamine against cisplatin-induced damage in auditory cells, the organ of Corti from postnatal rats (P2) and adult Balb/C mice. Cisplatin increases the catalytic activity of caspase-3 and caspase-8 proteases and the production of free radicals, which were significantly suppressed by pretreatment with bucillamine. Bucillamine induces the intranuclear translocation of Nrf2 and thereby increases the expression of γ-glutamylcysteine synthetase (γ-GCS) and glutathione synthetase (GSS), which further induces intracellular antioxidant glutathione (GSH), heme oxygenase 1 (HO-1) and superoxide dismutase 2 (SOD2). However, knockdown studies of HO-1 and SOD2 suggest that the protective effect of bucillamine against cisplatin is independent of the enzymatic activity of HO-1 and SOD. Furthermore, pretreatment with bucillamine protects sensory hair cells on organ of Corti explants from cisplatin-induced cytotoxicity concomitantly with inhibition of caspase-3 activation. The auditory-brainstem-evoked response of cisplatin-injected mice shows marked increases in hearing threshold shifts, which was markedly suppressed by pretreatment with bucillamine <I>in vivo</I>. Taken together, bucillamine protects sensory hair cells from cisplatin through a scavenging effect on itself, as well as the induction of intracellular GSH.</P>

고속철도 건설과 관련하여 지하수위 분포를 이용한 지하수환경 변화 분석

함세영,류상민,정재열,강래수,문상호,황학수 부산대학교 환경문제연구소 2002 環境硏究報 Vol.20 No.-

This study is aimed for estimating change of groundwater environment in relation to constructing Honam express railway in Cheonan-Iksan section. This study was conducted by measuring depth to water and by calculating groundwater level anomaly. Depth to water ranges from 0.03 to 21.1 m and ranges from 0.17 to 227.95 m mean sea level. Distributions of depth to water, of groundwater elevation and of groundwater level anomaly were analyzed to estimate water-level change due to express railway construction. High water-level anomaly section is found in Jangjae(Cheonan)-Daejeon-Iksan section with anomaly index 85.39. High water-level change is anticipated in 22.22-24.19 km, 25.1-25.53 km, 25.53-26.46 km, 26.46-27.77 km, 28.7-29.29 km, 30.18-30.26 km, and 30.26-32.4 km sections from the start of Jangjae(Cheonan).

뇌동맥류환자에서의 나선식 CT를 이용한 3D CT 혈관조영술의 이용 및 가치

박세혁,이규호,오세문 대한신경외과학회 1996 Journal of Korean neurosurgical society Vol.25 No.11

We evaluate the detection of cerebral aneurysms with 3-dimensional(3D) computed tomographic angiography(CTA) and compare the results with those of magnetic resonance angiography(MRA) and conventional cerebral angiography(CCA). Ten patients with 12 cerebral aneurysms were studied with spiral CT in conjunction with CCA and MRA. All aneurysms(3-20㎜ in diameter) were seen in CTA, MRA and CCA except 1 aneurysm(3㎜ in diameter) in MRA. CIA demonstrated neck, shape, direction of the aneurysm, and adjacent vascular and bony structures well. Of 12 aneurysms. 4 were seen equally with CCA and MRA. 4 were seen better with CCA owing to flow-related or motion artifact in MRA, and 4 were seen better with MRA. CTA was considered to be superior to CCA and MRA in demonstrating calcification and thrombosis in the aneurysm. 3D CTA with spiral CT is a rapid noninvasive method of visualizing the cerebral aneurysms and is comparable with MRA in demonstrating the aneurysms. We think 3D CTA can be a screening tool for use in the patients with suspected cerebral aneurysms and will be of a value in surgical planning for patients with complex cerebral aneurysms, in demonstrating the anatomy of the aneurysms and surrounding structures.

삼각측량 시스템을 이용한 물체의 3-D 정보추출 관한 연구

이호영,김국세,문정환,이철승,방극인,박영옥 조선대학교 전자정보통신연구소 2002 電子情報通信硏究所論文誌 Vol.5 No.2

The 3-D shape use to effect of movie, animation, industrial design, medical treatment service, education, engineering etc... But it is not easy to make 3-D shape from the information of 2-D image. There are two methods in restoring 3-D video image through 2-D image; First the method of using a laser; Second, the method of acquiring 3-D image through stereo vision. Instead of doing two methods with many difficulties, I study the method of simple 3-D image in this research paper. We present here a simple and efficient method, called direct calibration, which does not require any equations at all. The direct calibration procedure builds a lookup table(LUT) linking image and 3-D coordinates by a real 3-D triangulation system. The LUT is built by measuring the image coordinates of a grid of known 3-D points, and recording both image and world coordinates for each point; the depth values of all other visible points are obtained by interpolation.

두개골조기봉합증환자에서 3차원단층촬영영상의 이용

박세혁,윤대영,이규호,오세문 대한신경외과학회 1996 Journal of Korean neurosurgical society Vol.25 No.7

Technical advances in computer image analysis have made possible three-dimensional(3D) surface reformations of anatomic structures from contiguous axial CT slices We have recently utilized this 3D CT reconstruction with spiral CT scanner in S consecutive patients with primary or secondary craniosynostosis to assess the pres-ence and extent of synostosis. associated calvarial deformity and to facilitate surgical planning of ths craniectomy sits High resolution 3- mm contiguous CT sections were obtained and 3D shaded-surface reconstruction image gen-erated after reformation at f- mm interval In each case 9D CT demonstrated the exact extent of synostosis. and in 3 cases it was confirmed by surgical inspection 3D CT lmages facilitated surgical planning by precisely localizing the proper sits for craniectomy Three-dimensional CT with shaded-surface display may be a us㉣ful adjunct to im-aging and surgical planning in the patients with craniosynostosis. as well as a useful follow-up imaging study during observation or after surgery

韓國産 달팽이의 化學成分에 관한 硏究 : (1) 민달팽이와 달팽이의 無機質 및 아미노酸組成 (1) Mineral Content and Amino Acid Composition of Korean Slug and Korean Land Snail

金世權,李應昊,文聖勳,姜玉珠 釜山水産大學校 1983 釜山水産大學 硏究報告 Vol.23 No.2

민달팽이 및 달팽이의 化學成分을 分析하여 營養學的 價値 및 漢方效果를 究明하기 위한 基礎資料를 얻고자 一般成分, 無機質, 構成아미노酸 및 遊離아미노酸을 分析한 結果는 다음과 같다. 민달팽이 및 달팽이의 蛋白質含量은 各各 11.6%, 14.7%였으며 灰分含量은 2.7% 및 2.1%이었다. 민달팽이의 無機物 중 K이 213.0㎎%로 가장 含量이 많았고, Na, P 및 Ca의 含量은 各各 142.9㎎%, 138.6㎎% 및 129.5㎎% 였다. Cu, Fe, Zn 및 Mn은 微量元素로서 檢出되었지만 Fe含量은 29.63㎎%로 매우 높은 含量이었다. 달팽이의 경우도 K이 288.2㎎%로 가장 그 含量이 많았고, 다음은 P이 173.7㎎%, Ca은 97.2㎎%, Na은 51.4㎎%, Mg은 30.8㎎%이었다. 微量元素 중 Zn 및 Fe含量은 各各 16.58㎎%, 8.64㎎%이었다. 構成아미노酸은 乾物重量으로서 민달팽이의 40.9%였고, glutamic acid, aspartic acid, leucine, arginine, lysine 및 glycine이 全體아미노산의 53.4%로 함량이 많았으며, cystine, methionine 및 hictidine은 그 含量이 적었다. 必須아미노酸 含量은 總아미노산의 33.0%를 차지하였으며, 이와같은 傾向은 달팽이의 경우도 비숫한 傾向이었다. 민달팽이의 遊離아미노酸 중 含量이 많은 것은 arginine, glutamic acid, alanine 및 serine이었고, 이들이 全體 아미노酸의 54.8%이었으며 반면에 methionine, tyrosine은 含量이 적었으며, 全體 遊離아미노酸 含量은 711.3㎎/100g이었다. 달팽이의 遊離아미노酸 중 含量이 많은 것은 alanine, glutamic acid, arginine, lysine이었으며 이들이 全體 아미노산의 53.6%를 차지한 반면 mthionine, tyrosine 및 cystine은 매우 낮은 含量이었으며 全體 遊離아미노酸含量은 1052.2㎎/100g이었다. The proximate compositions, mineral contents and amino acid compositions of Korean slug, Incilaria frusferi, and Korean land snail, Acusta despecta(GRAY), were determined. The contents of protein, lipid and ash in Korean slug were 11.6%, 2.8% and 2.7%, and those from Korean land snail were 14.7%, 2.2% and 2.1%, respectively. Among the minerals in Korean slug, potassium, sodium and calcium were 213.0 mg%, 142.9 mg% and 129.5 mg%, and those from Korean land snail were 288.2 mg%, 51.4 mg% and 97.2mg%, respectively. In the case of trace element in Korean slug, iron, manganese and copper were 29.63 mg%, 11.21 mg% and 1.64 mg%, and those from Korean land snail were 6.84 mg%, 6.85 mg%, and 2.74 mg%, respectively. The protein of Korean slug was composed of glutamic acid and aspartic acid as a major amino acids and considerable amount of leucine, arginine, lysine and glycine. The amount of essential amino acids except tryptophan was 40.5% of total amino acid. The amino acid composition of Korean land snail was similar to those of Korean slug. Among the free amino acid in Korean slug, arginine, glutamic acid, alanine and serine showed high quantity and the sum of these amino acids occupied 54.8% of total free amino acid. While methionine and tyrosine were poor in content. In the Korean land snail, alanine, glutamic acid, arginine and lysine were abundant amino acids, and the sum of these amino acids occupied 53.6% of total free amino acid. The quantities of methionine, tyrosine and cystine were, in contrast, very small.

고저항 지대에서의 코어링 접지공사에 관한 연구

김세호,김일환,양문길 제주대학교 공과대학 첨단기술연구소 2002 尖端技術硏究所論文集 Vol.13 No.2

The resistivity of the soil depends on many parameters. It depends on the type of soil and therefore varies with distance as well as with depth. Especially soil resistivity of volcanic area is very high and it is difficult to acquire necessary grounding resistivity. This paper introduces the efficient grounding construction using coring technique for proper grounding resistance in high resistivity area. Because it is difficult to measure the accurate resistivity of soils, their resistivities are estimated using measured value of ground resistance and equivalent radius calculation method. Estimated resistivities are used for grounding resistance in scheduled grounding region.

홍피증 환자 39명에 대한 임상 소견 및 원인 규명에 관한 연구

안세진,장경애,문기찬,성경제,최지호,고재경 대한피부과학회 1999 大韓皮膚科學會誌 Vol.37 No.5

Background: Erythroderma may result from different causes. Almost all the published original clinical series of erythroderma originated from western countries. Objectives: The objective was to evaluate various causes of erythroderma in our environment, frequency, clinical and laboratory findings, and patient evolution. Method: We reviewed the clinical and laboratory findings including biopsy materials of 39 patients diagnosed as erythroderma and treated during the last 9 years (from 1989 to 1998). Results: The male-female ratio was 3 to 1. The mean age at diagnosis was 54 years. Drug reaction and undetermined cause were the most frequent cause (30.8%, respectively), followed by the pre-existing dermatoses (28.2%), and malignancies (10.3%). Elevated erythrocyte sedimentation rate was found in 70.4% of the patients. Eight patients were resistant to treatment. In the patients with the persistent erythroderma, leukocytosis, elevated erythrocyte sedimentation rate, and cosinophilia were the common findings. Conclusions: Half of the patients with the erythroderma of undetermined cause showed the protracted course. Two patients diagnosed as cutaneous T-cell lymphoma and Se´zary syndrome had the history of generalized erythroderma of more than 3 years. The close follow-up for the erythroderma of undetermined cause with frequent laboratory and histopathological evaluations would be mandatory.

뇌열환자에서 임상증상과 영상소견과의 관계 : Correlation of Clinical Outcome with Imaging Findings

박세혁,조용준,이규호,오세문 대한신경외과학회 1996 Journal of Korean neurosurgical society Vol.25 No.12

Schizencephaly is an infrequent congenital disorder of neuronal migration characterized by gray matter-lined clefts that extend through the entire cerebral hemisphere, from the ependymal lining of the lateral ventricle to the pial covering of the cortex. We have studied seven patients with schizencephaly rstrospectively to correlate clinical outcome with the type, size, and location of the clefts and to find associated brain anomalies. Three patients had bilateral clefts(one with two open lip clefts and the other two with a left open lip cleft and a right closed lip cleft), another two patients had left unilateral open lip clefts, and the rest two patients had right closed lip clefts. Clinically these patients presented motor dysfunction such as hemiparesis, seizures, and variable developmental delay. Patients with bilateral clefts, paticularly open lip type had worse motor and developmental impairment than those with unilateral clefts. Patient with unilateral medium open lip cleft had worse motor and intellectual impairment than those with unilateral closed lip clefts or small open lip cleft. All patients with f개ntal lobe involvement had motor dysfunction. Absence of septum pellucidum, gray matter heterotopia, polymicrogyria, and hypoplasia of optic nerves were also frequently found to be associated with schizencephaly. Three patients with skull changes such as enlargement of the hemicranium of erosion of the inner table of the skull were shunted but only one patient improved in the imaging study We conclude that the severity of patient's symptoms is related to the amount type and location of the involved brain.

敗令散 및 補中治濕湯이 Puromycin Aminonucleoside로 誘發된 白鼠의 腎症에 미치는 影響

梁文浩,曺東鉉,安世永,鄭定烈,杜鎬京 慶熙大學校韓醫科大學韓醫學硏究所 1995 慶熙韓醫大論文集 Vol.18 No.2

The effects of Paeryungsan and Bojungchiseubtang on rats with nephrosis induced by a single intravenous injection of puromycin aminonucleoside(PAN), 2.5mg/100g of body weight were evaluated in the present study. The effects of Paeryungsan and Bojungchiseubtang on PAN nephrosis were evaluated by measuring ①the concentrations of albumin, total protein, total lipid, cholesterol, triglyceride, creatinine, blood urea nitrogen and uric acid in the serum, ②the amount of volume, protein, glucose and creatinine of the 24hours urine ③the concentration of blood in the urine, intake water, and by observing the changes of microscopic findings of kidney. The results are summerized as follows; 1. In the control group, the concentrations of cholesterol, creatinine, blood urea nitrogen in the serum, the amount of protein and glucose of 24 hours urine, creatinine of 24 hours urine and 24 hours urine volume on the 22th day were significantly increased. On the other hand, the concentration of albumin in the serum, the amount of creatinine of 24 hours urine on the 8th day, the volume of 24 hours urine and the intake water on the 8th, 15th day were decreased significantly. Electron microscopic findings were the segmental obliteration of the foot processes, swelling and villous transformations of the visceral epithelial cells and partial increase of the mesangial matrix. 2. In Paeryungsan group, the decrease of albumin in the serum and intake water were inhibited significantly. But the increase of the concentrations of cholesterol and blood urea nitrogen in the serum were inhibited significantly. And the amount of creatinine of 24 hours urine was increased significantly. There was no significant difference between Paeryungsan group and the control group in electron microscopic findings. 3. In Bojungchiseubtang group, the decrease of albumin in the serum and intake water were inhibited significantly. But the increase of the concentrations of cholesterol, total lipid, creatinine, blood urea nitrogen in the serum and the amount of protein in the 24 hours urine were inhibited significantly. The concentration of protein in the serum was increased significantly. There was no significant difference between Bojungchiseubtang group and the control group in electron microscopic findings. To conclude, PAN nephrosis was induced by a single intravenous injection of PAN 2.5mg/100g of body weight on rats. It can be inferred that Paeryungsan group has the effects of improving hypoalbuminemia in nephrotic syndrome and relieving azotemia when nephrotic syndrome is accompanied by the acute renal failure. It can be inferred that Bojungchiseubtang may improve proteinuria, hypoalbuminenia, hyperlipidemia in nephrotic syndrome. But there was no significant difference between Paeryungsan and the control group, between Bojungchiseubtang group and the control group in electron microscopic findings. So I hope that there will be further studies on the effect on the alterations of glomerular polyanion sites, and on the functioning mechanism of Paeryungsan and Bojungchiseubtang in the future.