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Saxena Shipra,Reddy K.R.K.,Rajam M.V. 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.2
Chitin, the second most abundant structural amino polysaccharide in nature, is widely present in insects forming the cuticle and peritrophic membrane. Chitin polymers are formed by polymerization of UDP-Nacetylglucosamine units by chitin synthase (CHS), a conserved enzyme found in all chitin-synthesizing organ isms. In most insect species, two isoforms of CHS have been characterised, namely CHSA, involved in cuticular chitin synthesis and CHSB, involved in formation of the PM. In the present study, the said two isoforms of CHS gene have been identified in Leucinodes orbonalis. Isoforms were found to be differentially expressed at different developmental stages of the insect and down-regulation of the CHSA isoform, manifested an extended larval period, mortality at larval stage as well as reduction in fecundity of the insect pest. Further, systemic RNA interference defective protein (SID), a protein involved in dsRNA transport, was also isolated and was found to be differentially expressed across developmental stages of L. orbonalis. Also, dsRNA supplementation leads to an upregulation in SID transcript levels, suggestive of its involvement in dsRNA transport and processing.