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      저자 : Sasi Jyothsna, T. S. (검색결과 1 건)
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      • Shewanella fodinae sp. nov., isolated from a coal mine and from a marine lagoon

        Sravan Kumar, R.,Sasi Jyothsna, T. S.,Sasikala, Ch.,Seong, C. N.,Lim, C. H.,Park, S. C.,Ramana, Ch. V. Microbiology Society 2010 International journal of systematic and evolutiona Vol.60 No.7

        <P>Strains JC15<SUP>T</SUP> and JC19 were isolated from samples collected from different locations in India, including a coal mine and a marine lagoon. Both strains were Gram-stain-negative rods, motile by means of a single polar flagellum, catalase- and oxidase-positive, and hydrolysed casein, produced H2S and showed <I>β</I>-haemolysis. Strain JC15<SUP>T</SUP> grew optimally at pH 6 (range pH 5-8) while strain JC19 grew optimally at pH 7 (range pH 6-9) and both had a growth temperature optimum of 30-37 °C (range 22-40 °C). Both strains could grow chemo-organoheterotrophically and chemolithoautotrophically. Neither strain required NaCl for growth and both could tolerate up to 9 % (w/v) NaCl, with optimum growth at 5 % NaCl. Vitamin B12 was required as a growth factor by both strains. The major fatty acids were iso-C15 : 0, C17 : 1<I>ω</I>8<I>c</I> and iso-C13 : 0 3-OH. The DNA G<I>+</I>C contents of strains JC15<SUP>T</SUP> and JC19 were 53.6 and 54.3 mol%, respectively. A phylogenetic tree based on 16S rRNA gene sequence analysis showed that strains JC15<SUP>T</SUP> and JC19 were most closely related to <I>Shewanella haliotis</I> DW01<SUP>T</SUP> (approximately 94 % sequence similarity) and to other members of the genus <I>Shewanella.</I> Genomic relatedness (DNA-DNA hybridization) between strains JC15<SUP>T</SUP> and JC19 is 88 %. On the basis of phenotypic and molecular genetic evidence, strain JC15<SUP>T</SUP> represents a novel species of the genus <I>Shewanella</I>, for which the name <I>Shewanella fodinae</I> sp. nov. is proposed. The type strain is JC15<SUP>T</SUP> (=CCUG 57102<SUP>T</SUP> =NBRC 105216<SUP>T</SUP> =KCTC 22506<SUP>T</SUP>).</P>

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