Session 9 Distribution Science(Student-Myongii University)-VIII : Graduate School of Distribution

Ahn,Sung-Woo,Kim,Dae-yun,Kim,Mi-Song,Kim,Han-Sung,Jung,Je-Woo,Song,Ha-Su,Eom,Heung-Jun 한국유통과학회 2008 한국유통과학회 학술대회 논문집 Vol.2008 No.-

일반논문 : 판소리 수궁가 의학기사(醫學記事)에 내포된 역사성과 조선후기 민중 의학지식의 보급 -김연수 창본 수궁가의 사설을 대상으로-

인상우 ( Sang Woo Ahn ) 전남대학교 호남학연구원 2010 호남학 Vol.0 No.47

<수궁가>는 구토설화에서 파생된 판소리 창본으로 내용 중 醫學記事가 자주 등장한다. 醫學記事는 신재효의 창본정리 과정에서 <수궁가> 전반에 삽입된 것으로 보이는데, 지방관청에 소속된 官藥房을 했던 부친의 영향으로 『東醫寶鑑』과 여러 의서들, 藥器를 쉽게 접할 수 있었던 까닭에 <수궁가> 내에 그 내용을 충분히 실어 넣은 것으로 보인다. 본고에서 진행한 <수궁가>의 의학적 분석을 통한 연구는 <수궁가>에서 절반 가까이 차지하는 藥性歌 및 醫學記事의 많은 부분이 한의학 지식과 매우 일치하며, 내용전반에 민간에서 쓸 수 있는 한의학 지식을 바탕에 깔고 있음을 확인할 수 있었다. 특히 『東醫寶鑑』이 점차 보편화되면서 <수궁가> 속의 醫學記事도 전문성을 더해갔다. <수궁가>에서 도가적 양생사상의 신선술과 주술에 의거한 치료형태를 풍자하고 희화시켰던 양상은, 동시기에 『東醫寶鑑』이 여러 의서를 종합하면서 도가적인 주술이나 미신적인 요소를 배제하고, 체계적이고 임상의학적으로 재정비했다는 점과 맥을 같이한다. 18세기중반 이후로 19~20세기 양반지식층의 애호참여로 유교사상을 재정비하면서 醫學記事가 전문지식의 과시수단으로 사용되기도 하였으나, 한편으로는 전문성있는 의학지식을 판소리내에 삽입함으로써 판소리에 사실성을 부여하고, 청자들로 하여금 판소리의 현실감을 극대화하는 역할로 작용하였다. 그로인해 <수궁가>내에서 더욱 전문화된 醫藥記事들은 이후 판소리, 국문한문소설로 변화되어 보급되었고, 19세기부터는 라디오매체와 음반사의 판소리 취입으로 더욱 보편화되면서 서민들의 입과 귀로 널리 전해졌다. 비록 醫學記事가 서민들에게 의학지식의 보급을 목적으로 작성된 것은 아니었다 하더라도, 의료정책이 미비했던 당시 『東醫寶鑑』을 바탕으로 보완되었던 <수궁가>의 보급은 원래의 목적이상으로 민중들에게 민간의학지식을 파급시키는 역할을 수행했다. Sugungga is a Pansori[recite a dramatic song] story derived from a tale about a terrapin and a rabbit, and Yakseongga appears in its first part. Sugungga has been handed down through a lineage of singers: Song Heung-Rok, Song Gwang-Rok, Song Woo-Ryong, Ryu Seong-Jun, and Kim, Yeon-Su. Yakseongga completely settled in Sugungga thanks to adaptation of Shin Jae-Hyo, whose medicinal knowledge was mostly from his father, Shin Gwang-Heup, an official pharmacist in a local government office. Between the 17th century and the mid-18th century, Sugungga was evolved from ancient taoistic idea of health to Confucianistic rational subject. This was similar in nature that Korean medicine was overhauled to a more scientific and clinical practice in the same period by putting together diverse medicinal books such as DongeuiBogam, Huangdi Neijing, Hyang-yak Jipseongbang, Euibang Ryuchwi, and Euibang Chalyo, while eliminating elements of taoistic charms or superstitions. This study confirmed that from the study of oriental medicine, Yakseongga in Sugungga was based on oriental medicinal knowledge, not simple humor. It seems that Yakseongga was heavily influenced by oriental medicinal books such as Huangdi Neijing, Hyang-yak Jipseongbang, Dongeui Bogam, and Jejung Sinpyeon. After the mid-18th century, deconfucianistic Sugungga was modified again. Participation of aristocratic intellectuals from the 19th and 20th Century tarnished lively popularity of Sugungga, while Confucianism was reintroduced and “ostentation of expertise” became more noticeable in Yakseongga. Such trends of pansori became more popular following the introduction of modern novels as well as radios and phonograph records. Although Yakseongga was not made to teach medicinal knowledge, it performed a role of distributing folk remedies to the public far beyond its original intention when it had been compiled based on Dongeui Bogam and Jejung Sinpyeon.

국내 한의학 학술지에 발표된 동의보감 연구 현황 조사

한창현(Chang-hyun Han),박상영(Sang-young Park),권오민(Oh-min Kwon),안상영(Sang-young Ahn),안상우(Sang-woo Ahn) 한국의사학회 2009 한국의사학회지 Vol.22 No.2

Background : To understand Korean medicine it is crucial to first understand medical aspect of ‘DongUiBoGam’. It is also meaningful that it became one of the influential book even to posterity. Also based on the apprehension, we can take a view of development of Korean medicine led by ‘DongUiBoGam’. Objectives : This study aims to review the status, study field, specialist of DongUiBoGam. In the process, this review will grasp trends in this field of studies and will direct further researches into the right direction. Method : The computerized Korean databases were searched from their respective inceptions up to December 2008. The search terms used were ‘DongUiBoGam’ and random or Korean language terms related to DongUiBoGam. Several specialized journals were also manually searched for relevant articles. Result : Since the 2000s, DongUiBoGam papers in the Korean Literature is increased. Published 58 papers on The Korean Journal of Oriental Medical Prescription were more than the other journals. 58 papers published in The Korean Journal of Oriental Medical Prescription are the best in many areas related to Korean medicine. Most people have submitted papers related to DongUiBoGam was Woo-yeal Jeong. Conclusions : ‘DongUiBoGam’ research is conducted and current tendency and outlook for ‘DongUiBoGam’ is carried out focusing on several associations.

Continuous Nondestructive Monitoring Method Using the Reconstructed Three-Dimensional Conductivity Images via GREIT for Tissue Engineering

Ahn, Sujin,Wi, Hun,Oh, Tong In,McEwan, Alistair Lee,Jun, Sung Chan,Woo, Eung Je Hindawi Limited 2014 Journal of applied mathematics (JAM) Vol.2014 No.-

<P>A continuous Nondestructive monitoring method is required to apply proper feedback controls during tissue regeneration. Conductivity is one of valuable information to assess the physiological function and structural formation of regenerated tissues or cultured cells. However, conductivity imaging methods suffered from inherited ill-posed characteristics in image reconstruction, unknown boundary geometry, uncertainty in electrode position, and systematic artifacts. In order to overcome the limitation of microscopic electrical impedance tomography (micro-EIT), we applied a 3D-specific container with a fixed boundary geometry and electrode configuration to maximize the performance of Graz consensus reconstruction algorithm for EIT (GREIT). The separation of driving and sensing electrodes allows us to simplify the hardware complexity and obtain higher measurement accuracy from a large number of small sensing electrodes. We investigated the applicability of the GREIT to 3D micro-EIT images via numerical simulations and large-scale phantom experiments. We could reconstruct multiple objects regardless of the location. The resolution was 5 mm<SUP>3</SUP>with 30 dB SNR and the position error was less than 2.54 mm. This shows that the new micro-EIT system integrated with GREIT is robust with the intended resolution. With further refinement and scaling down to a microscale container, it may be a continuous nondestructive monitoring tool for tissue engineering applications.</P>

UPnP SDK for Robot Development

Sang Chul Ahn,Jung-Woo Lee,Ki-Woong Lim,Heedong Ko,Yong-Moo Kwon,Hyoung-Gon Kim 제어로봇시스템학회 2006 제어로봇시스템학회 국제학술대회 논문집 Vol.2006 No.10

Optimization for Axial Resolution, Depth Range, and Sensitivity of Spectral Domain Optical Coherence Tomography at 1.3 μm

Sang-Won Lee,Hyun-Woo Jeong,Beop-Min Kim,Yeh-Chan Ahn,Woonggyu Jung,Zhongping Chen 한국물리학회 2009 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.55 No.6

We have developed high-speed and high-resolution spectral domain optical coherence tomography at 1.3 μm using an InGaAs line-scan camera and a broadband light source with the bandwidth of 170 nm that produces a theoretical axial resolution of 4.4 μm in air. We compared axial resolutions from point spread functions (PSFs) and depth ranges while changing the full spectral bandwidth detected by the camera and describing the optimization process for the axial resolution, the depth range, and the sensitivity for SD-OCT system. We found that SD-OCT at 1.3 μm cannot satisfy the conditions both below the axial resolution of 5 μm and above the depth range of 2 mm because of the restricted pixel number of the line-scan camera. To scan a large depth range, the axial resolution has to be sacrificed. In addition, the sensitivity rolls off slowly as a function of the depth if a large depth range is scanned. On the other hand, if the axial resolution needs to be close to the theoretical one, the depth range becomes limited and the sensitivity decays quickly. Since we have to maintain a reasonable depth range of 2.0 mm, we chose the spectrum full bandwidth of 214 nm captured by the detector to balance the axial resolution of 8.2 μm. In this setting, the sensitivity of our OCT system was measured at 107.1 dB. Theoretical and experimental results are compared and presented in this paper. We have developed high-speed and high-resolution spectral domain optical coherence tomography at 1.3 μm using an InGaAs line-scan camera and a broadband light source with the bandwidth of 170 nm that produces a theoretical axial resolution of 4.4 μm in air. We compared axial resolutions from point spread functions (PSFs) and depth ranges while changing the full spectral bandwidth detected by the camera and describing the optimization process for the axial resolution, the depth range, and the sensitivity for SD-OCT system. We found that SD-OCT at 1.3 μm cannot satisfy the conditions both below the axial resolution of 5 μm and above the depth range of 2 mm because of the restricted pixel number of the line-scan camera. To scan a large depth range, the axial resolution has to be sacrificed. In addition, the sensitivity rolls off slowly as a function of the depth if a large depth range is scanned. On the other hand, if the axial resolution needs to be close to the theoretical one, the depth range becomes limited and the sensitivity decays quickly. Since we have to maintain a reasonable depth range of 2.0 mm, we chose the spectrum full bandwidth of 214 nm captured by the detector to balance the axial resolution of 8.2 μm. In this setting, the sensitivity of our OCT system was measured at 107.1 dB. Theoretical and experimental results are compared and presented in this paper.

Analysis of Electro-Optic Properties of a Polymer Network Liquid Crystal Display with Crossed Polarizers

Woo, Sung-Ho,Jeon, Chan-Wook,Yang, Kee-Jeong,Choi, Byeong-Dae,Rajesh, Kumar,Ahn, Byung-Chul TaylorFrancis 2007 MOLECULAR CRYSTALS AND LIQUID CRYSTALS - Vol.470 No.1

<P> An active-matrix polymer network liquid crystal display (AMPNLCD) of 1.79'' QCIF(176 × 144) was fabricated. The operating mode of normally black was reversed to normally white by using crossed polarizers. The contrast ratio, as a result, was drastically increased, which was comparable to a conventional liquid crystal display. The response time at a typical driving voltage, 2.8 V, was also fast enough to play still image smooth. The electro-optic properties of AMPNLCD with crossed polarizers were evaluated and discussed.</P>

Effect of culture temperature on erythropoietin production and glycosylation in a perfusion culture of recombinant CHO cells

Ahn, Woo Suk,Jeon, Jae-Jin,Jeong, Yeong-Ran,Lee, Seung Joo,Yoon, Sung Kwan Wiley Subscription Services, Inc., A Wiley Company 2008 Biotechnology and bioengineering Vol.101 No.6

<P>To investigate the effect of culture temperature on erythropoietin (EPO) production and glycosylation in recombinant Chinese hamster ovary (CHO) cells, we cultivated CHO cells using a perfusion bioreactor. Cells were cultivated at 37°C until viable cell concentration reached 1 × 10<SUP>7</SUP> cells/mL, and then culture temperature was shifted to 25°C, 28°C, 30°C, 32°C, 37°C (control), respectively. Lowering culture temperature suppressed cell growth but was beneficial to maintain high cell viability for a longer period. In a control culture at 37°C, cell viability gradually decreased and fell below 80% on day 18 while it remained over 90% throughout the culture at low culture temperature. The cumulative EPO production and specific EPO productivity, q<SUB>EPO</SUB>, increased at low culture temperature and were the highest at 32°C and 30°C, respectively. Interestingly, the cumulative EPO production at culture temperature below 32°C was not as high as the cumulative EPO production at 32°C although the q<SUB>EPO</SUB> at culture temperature below 32°C was comparable or even higher than the q<SUB>EPO</SUB> at 32°C. This implies that the beneficial effect of lowering culture temperature below 32°C on q<SUB>EPO</SUB> is outweighed by its detrimental effect on the integral of viable cells. The glycosylation of EPO was evaluated by isoelectric focusing, normal phase HPLC and anion exchange chromatography analyses. The quality of EPO at 32°C in regard to acidic isoforms, antennary structures and sialylated N-linked glycans was comparable to that at 37°C. However, at culture temperatures below 32°C, the proportions of acidic isoforms, tetra-antennary structures and tetra-sialylated N-linked glycans were further reduced, suggesting that lowering culture temperature below 32°C negatively affect the quality of EPO. Thus, taken together, cell culture at 32°C turned out to be the most satisfactory since it showed the highest cumulative EPO production, and moreover, EPO quality at 32°C was not deteriorated as obtained at 37°C. Biotechnol. Bioeng. 2008;101: 1234–1244. © 2008 Wiley Periodicals, Inc.</P>

Changes of Ganglioside GM3 Expression in Porcine Oocyte Maturation and Early Embryonic Development In Vitro

Sung-Kyu Chae,Hyo-Jin Park,Jin-Woo Kim,Jae-Hyun Ahn,Soo-Yong Park,Jae-Young Park,Seul-Gi Yang,Deog-Bon Koo 한국수정란이식학회 2015 한국동물생명공학회지 Vol.30 No.4

Gangliosides exist in glycosphingolipid-enriched domains on the cell membrane and regulate various functions such as adhesion, differentiation, and receptor signaling. Ganglioside GM3 by ST3GAL5 enzyme provides an essential function in the biosynthesis of more complex ganglio-series gangliosides. However, the role of gangliosides GM3 in porcine oocytes during in vitro maturation and early embryo development stage has not yet understood clear. Therefore, we examined ganglioside GM3 expression patterns under apoptosis stress during maturation and preimplantation development of porcine oocytes and embryos. First, porcine oocytes cultured in the NCSU-23 medium for 44 h after H2O2 treated groups (0.01, 0.1, 1 mM). After completion of meiotic maturation, the proportion MII (44 h) was significantly different among control and the H2O2 treated groups (76.8±0.3 vs 69.1±0.4; 0.01 mM, 55.7±1.0; 0.1 mM, 38.2±1.6%; 1 mM, P<0.05). The expressions of ST3GAL5 in H2O2 treated groups were gradually decreased compared with control group. Next, changes of ST3GAL5 expression patterns were detected by using immunofluorescene (IF) staining during preimplantation development until blastocyst. As a result, we confirmed that the expressions of ST3GAL5 in cleaving embryos were gradually decreased (P<0.05) according to the early embryo development progress. Based on these results, we suggest that the ganglioside GM3 was used to the marker as pro-apoptotic factor in porcine oocyte of maturation and early embryo production in vitro, respectively. Furthermore, our findings will be helpful for better understanding the basic mechanism of gangliosides GM3 regulating in oocyte maturation and early embryonic development of porcine in vitro.

3′-UTR Polymorphisms of <i>MTHFR</i> and <i>TS</i> Associated with Osteoporotic Vertebral Compression Fracture Susceptibility in Postmenopausal Women

Ahn, Tae-Keun,Kim, Jung Oh,Kim, Hyun Woo,Park, Han Sung,Shim, Jeong Hyun,Ropper, Alexander E.,Han, In Bo,Kim, Nam Keun MDPI 2018 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.19 No.3

<P>Postmenopausal osteoporosis is one of the most prominent diseases in postmenopausal women and it is increasing in prevalence with the aging population. Furthermore, osteoporosis and osteoporotic vertebral compression fractures (OVCFs) are related to mortality and decreased quality of life. Therefore, searching for biomarkers that are able to identify postmenopausal women who are at high risk of developing OVCFs is an effective strategy for improving the quality of life of patients and alleviating social and economic burdens. In this study, we investigated methylenetetrahydrofolate reductase (<I>MTHFR</I>) and thymidylate synthase (<I>TS</I>) gene polymorphisms in postmenopausal women with OVCF. We recruited 301 postmenopausal women and performed genotyping for the presence of <I>MTHFR</I> 2572C>A, 4869C>G and TS 1100C>T, 1170A>G. Genotyping was analyzed using the polymerization chain reaction restriction fragment length polymorphism assay. MTHFR 2572C>A and TS 1100C>T were associated with the prevalence of osteoporosis (MTHFR 2572CC versus CA+AA: odd ratio [OR] adjusted age, hypertention [HTN], and diabetes mellitus [DM] = 0.49, <I>p</I> = 0.012) and the occurrence of OVCFs (MTHFR 2572CC versus CA+AA: OR adjusted age, HTN, and DM = 0.38, <I>p</I> = 0.013; TS 1100CC versus CT+TT: OR adjusted age, HTN, and DM = 0.46, <I>p</I> = 0.02). Our novel finding is the identification of MTHFR and TS genetic variants that decrease susceptibility to OVCFs. Our findings suggest that polymorphisms in the <I>MTHFR</I> and <I>TS</I> genes are associated with susceptibility to osteoporosis and OVCFs in postmenopausal women.</P>