The Variation of Winter Buds among 10 Selected Populations of Kalopanax septemlobus Koidz. in Korea

Kim,Sea-Hyun,Ahn,Young-sang,Jung,Hyun-Kwon,Jang,Yong-Seok,Park,Hyung-Soon 한국자원식물학회 2002 Plant Resources Vol.5 No.3

The objective of this study was to understand the conservation of gene resources and provide information for mass selection of winter bud characters among the selected populations of Kalopanax septemlobus Koidz using analysis of variance(ANOVA) tests. The obtained results are shown below; 1. Ten populations of K. septemlobus were selected for the study of the variation of winter bud characters in Korea. The results of the analysis of variance(ANOVA) tests shows that there were statistically significant differences in all of the winter bud characters among those populations. 2. Correlation analysis shows that width between Height and DBH(Diameter at breast height) characters have negative relationship with all of the characters, as ABL(Apical branch length), ABW(Apical branch width), AWBL(Apical branch winter bud length), AWBW(Apical branch winter bud width), ABT(Apical branch No. of thorns), ABLB(Apical branch No. of lateral bud) and LBL(Lateral branch length), LBW(Lateral branch width), LBT(Lateral branch No. of thorns), LBLB(Lateral branch No. of lateral bud). 3. The result of principal component analysis(PCA) for winter buds showed that the first principal components(PC s) to the fourth principal component explains about 78% of the total variation. The first principal component(PC) was correlated with AWBW, LWBW, and LBL and the ratio of ABL/ABW and LBL/LBW out of 16 winter bud characters. The second principal component correlated with ABL, ABW, ABLB, LWBL(Lateral branch winter bud length), and LBW and the ratio of AWBL/AWBW. The third principal component correlated with ABL, ABW, LWBL, LBL, and the ratio of LBL/LBW. The fourth principal component correlated with LBL and the ratio of LWBL/LWBW(Lateral branch winter bud width), LBL/LBW. Therefore, these characters were important to analysis of the variation for winter bud characters among selected populations of K. septemlobus in Korea. 4. Cluster analysis using the average linkage method based on 10 selected populations for the 16 winter bud characters of K. septemlobus in Korea showed a clustering into two groups by level of distance 1.1(Fig. 3). As can be seen in Fig. 3, Group I consisted of three areas(Mt. Sori, Mt. Balwang and Mt. Worak) and Group Ⅱ contisted of seven areas(Suwon, Mt. Chuwang, Mt. Kyeryong, Mt. Kaji, Mt. Jiri, Muan, and Mt. Halla). The result of cluster analysis for winter bud characters corresponded well with principal component analysis, as is shown in Fig. 2.

Effects of Phenytoin and Diazepam on the Seizure Activity in the Cortical Dysplasia Animal Models

Kim, Si-Hyung,Choi, In-Sun,Cho, Jin-Hwa,Park, Eun-Ju,Jang, Il-Sung,Choi, Byung-Ju,Kim, Hyun-Jung,Kim, Young-Jin,Nam, Soon-Hyeun The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

Dysplasia-associated seizure disorders are markedly resistant to pharmacological intervention. Relatively little research has been conducted studying the effects of antiepileptic drugs(AEDs) on seizure activity in a rat model of dysplasia. We have used rats exposed to methylazoxymethanol acetate(MAM) in utero, an animal model featuring nodular heterotopia, to investigate the effects of AEDs in the dysplastic brain. Pilocarpine was used to induce acute seizure in MAM-exposed and age-matched vehicle-injucted control animals. Field potential recordings were used to monitor amplitude and numbers population spikes, and paired pulse inhibition in response to stimulation of commissural pathway. Two commonly used AEDs were tested: diazepam 5, 2.5mg/kg;phenytoin 40, 60mg/kg. Diazepam(DZP) and phenytoin(PHT) reduced the amplitude of population spike in control and MAM-exposed rats. However, the amplitude of population spike was nearly eliminated in control rats as compared to the MAM-exposed rats. Pharmaco-resistance was tested by measuring seizure latencies in awake rats after pilocarpine administration(320mg/kg, i.p.) with and without pretreatment with AEDs. Pre-treatment with PHT 60 mg prolonged seizure latency in control rats, but not in MAM-exposed animals. The main findings of this study are that acute seizures initiated in MAM-exposed rats are relatively resistant to standard AEDs assessed in vivo. These data suggest that animal model with cortical dysplasia can be used to screen the effects of potential AEDs.

Assessment of In Vitro Assay System for Thyroid HormoneDisruptors Using Rat Pituitary GH3 Cells

Hee Jin Kim1,Hae Young Park1,Jeonga Kim1,Il Hyun Kang2,Tae Sung Kim2,Soon Young Han2,Tae Seok Kang2,Kui Lea Park2,Hyung Sik Kim1 한국독성학회 2006 Toxicological Research Vol.22 No.4

The development of in vitro assays has been recommended to screening and test-ing the potential endocrine disruptors (EDs). These assay systems focus only on identifying thethe thyroid hormone (TH) disruptors. The aim of this study was to evaluate a test system to detectTH disruptors using rat pituitary tumor GH3 cells. The test system is based on the TH-dependentincrease in growth rate. As expected, L-3,5,3-triiodothyronine (T3) markedly induced a morphologicalchange in GH 3 cells from flattened fibroblastic types to rounded or spindle-shaped types. T3 stimu-lated GH3 cell growth in a dose-dependent manner with the maximum growth-stimulating effect9 M. In addition, T3 increased the release of growth hor-mone and prolactin into the medium of the GH3 cells culture. Using this assay system, the TH-dis-rupting activities of bisphenol A (BPA) and its related compounds were examined. BPA,dimethylbisphenol A (DMBPA), and TCI-EP significantly enhanced the growth of GH3 cells in therange of 1 × 10-5M to 1 × 10-6M concentrations. In conclusion, this in vitro assay system might bestandardization before it can be used as a broad-based screening tool.

부추 전용 포장 결속기 개발

김용석(Yong-Seok Kim),박성희(Sung-Hee Park),박태표(Te-Pyo Park),양순용(Soon-Young Yang) 대한기계학회 2008 대한기계학회 춘추학술대회 Vol.2008 No.5

An Allium-odorum is difficult material to handling, because it is soft and weak as mechanical. For bundling work of allium-odorum, we must grip in the bundle shape of unit weight, however, it is difficult that grip allium-odorum by hand because the bundle bulk is very big. Especially, in packing work, the bundle shape of allium-odorum package than other vegetables is very important, because difference of a price becomes much according to the bundle shape. In this paper, we propose bundling mechanism for a quadrangle shape in bundle shape, and semi-automatic bundling mechanism that makes use of a eccentric roller and a triangularity link. We carry out mechanical modeling and analysing respectively using the CATIA V5 and SimDesigner. We manufactured the prototype of the semi-automatic bundling machine, and got satisfied result through field test. And this machine will be commercialized and announced soon.

채소 자동결속기의 개발(II) : 시제품제작 및 성능검증

김용석,박성호,양순용,Kim, Yong-Seok,Park, Sung-Ho,Yang, Soon-Young 한국생산제조학회 2009 한국생산제조학회지 Vol.26 No.3

In this paper, it has been proposed an automatic vegetable bundling mechanism that makes use of heat melt sticking. The proposed mechanism is consisted of three modules for the rationality of manufacture. Design specification has been determined by the bundling mechanism that was optimized mechanically. And, it has been manufactured the prototype of the automatic bundling machine. It has been carried out directly the field test for verification of performance in farmhouse which produces vegetable. In field test, this bundling machine showed efficiency of about 3.6 times than the manual working by manpower, and other performance it got satisfied result. This automatic vegetable-bundling machine was already registered as the agricultural machine. and This machine will be commercialized soon.

채소 자동결속기의 개발(Ⅱ) : 시제품제작 및 성능검증

김용석(Yong-Seok Kim),박성호(Sung-Ho Park),양순용(Soon-Young Yang) 한국생산제조학회 2009 한국생산제조학회지 Vol.18 No.4

In this paper, it has been proposed an automatic vegetable bundling mechanism that makes use of heat melt sticking. The proposed mechanism is consisted of three modules for the rationality of manufacture. Design specification has been determined by the bundling mechanism that was optimized mechanically. And, it has been manufactured the prototype of the automatic bundling machine. It has been carried out directly the field test for verification of performance in farmhouse which produces vegetable. In field test, this bundling machine showed efficiency of about 3.6 times than the manual working by manpower, and other performance it got satisfied result. This automatic vegetable-bundling machine was already registered as the agricultural machine. and This machine will be commercialized soon.

Outcome of Multipair Donor Kidney Exchange by a Web-Based Algorithm

Kim, Beom Seok,Kim, Yu Seun,Kim, Soon Il,Kim, Myoung Soo,Lee, Ho Yung,Kim, Yong-Lim,Kim, Chan Duck,Yang, Chul Woo,Choi, Bum Soon,Han, Duck Jong,Kim, Yon Su,Kim, Sung Joo,Oh, Ha-Young,Kim, Dae Joong American Society of Nephrology 2007 Journal of the American Society of Nephrology Vol.18 No.3

<P>Donor kidney exchange is an established method to overcome incompatibility of donor-recipient pairs (DRP). A computerized algorithm was devised to exchange donor kidney and was tested in a multicenter setting. The algorithm was made according to the consensus of participating centers. It makes all possible exchange combinations not only between two incompatible DRP but also circularly among three DRP and selects an optimum set of exchange combinations, considering several factors that can affect the outcome of the exchanged transplant. The algorithm was implemented as a web-based program, and matching was performed five times. Fifty-three DRP were enrolled from five transplant centers. The numbers of DRP that were enrolled in each matching were 38 (25:13), 39 (34:5), 33 (31:2), 32 (28:4), and 34 (30:4) (carryover:newcomer). The numbers of generated exchange combinations were 4:11, 3:17, 2:12, 2:3, and 2:3 (two-pair exchange:three-pair exchange), and the numbers of DRP in selected exchange combinations were six, 12, six, five, and four in each matching. The numbers of DRP with blood type O recipient or AB donor were five and one, respectively, in selected exchange combinations. Six DRP of two-pair exchange combinations and six DRP of three-pair exchange combinations underwent transplantation successfully. Computerized algorithm of donor kidney exchange was tried not only between two incompatible DRP but also circularly among three DRP. It showed that the algorithm has potential to improve the outcome of donor kidney exchange, especially for disadvantaged DRP with blood type O recipients or AB donors.</P>

Subwavelength localization and toroidal dipole moment of spoof surface plasmon polaritons

Kim, Seong-Han,Oh, Sang Soon,Kim, Kap-Joong,Kim, Jae-Eun,Park, Hae Yong,Hess, Ortwin,Kee, Chul-Sik American Physical Society 2015 Physical review. B, Condensed matter and materials Vol.91 No.3

Gene flow from transgenic soybean, developed to obtain recombinant proteins for use in the skin care industry, to non-transgenic soybean

Kim Do Young,Eom Min Sik,Kim Hye Jin,Ko Eun Mi,Pack In-Soon,Park Jung-Ho,박기웅,Nam Kyong-Hee,Oh Sung Duk,Kim Jae Kwang,Seo Ju Seok,Kim Chang-Gi 한국응용생명화학회 2020 Applied Biological Chemistry (Appl Biol Chem) Vol.63 No.5

Soybean has been recognized as a useful platform for heterologous protein production. This study compared the pollen characteristics of transgenic and non-transgenic soybean and investigated the rate of gene flow from transgenic soybean events, developed to obtain recombinant proteins (such as human epidermal growth factor, insulin-like growth factor 1, or thioredoxin) for use in the skin care industry, to non-transgenic soybean under field conditions, and determined the distance at which gene flow could occur. The lack of significant differences in pollen grain size, viability and pollen germination rates between transgenic and non-transgenic cultivars indicates that the overexpression of transgenes did not alter pollen characteristics in soybean. The highest rates of gene flow from the three transgenic soybean events to non-transgenic soybean ranged from 0.22 to 0.46% at the closest distance (0.5 m). Gene flow was observed up to 13.1 m from the transgenic plots. Our data fell within the ranges reported in the literature and indicate that an isolation distance greater than at least 13 m from transgenic soybean is required to prevent within-crop gene flow in soybean. As the potential markets for transgenic crops as a recombinant protein factory increase, gene flow from transgenic to non-transgenic conventional crops will become a key decision factor for policy makers during the approval process of transgenic crops. Our study may provide useful baseline data for the prevention of transgenic soybean seed contamination caused by transgene flow.

The Effects of Aerobic Exercise on Changes of Blood Glucose Insulin, Lipid in NIDDM Patients

Sung, Hye Ryen,Hwang, Ji In,Ro, Soon Duck,Youn, Mi Suk,Kim, Eun Hee,Jung, Jung Hwa,Gong, Byong Hak,Chae, Jong Hoon,Park, Cheol Ho,Park, Hyun Tae,Park, Sang Kap 대한스포츠의학회 1998 대한스포츠의학회지 Vol.16 No.2

성인병 발병율의 증가원인은 식생활의 서구화에서 오는 영양과잉, 과학의 발달로 인한 낮은 신체활동에서 오는 운동부족과 스트레스의 증가와 같은 환경인자와 흡연, 음주 등을 들 수 있는데 이는 성인병의 예방과 치료를 위해서는 의학적 처치는 물론, 식이요법·운동요법·생활 습관의 개선 등 다각적인 대처가 필요하다. 본 연구는 NIDDM 환자에게 있어 유산소운동이 혈당, 인슐린, 혈청지질에 미치는 효과를 구명하고자 자전거 에르고미터 운동을 8주간, 주 3회, 1회 60분간 실시하였다. 체중은 64.8kg±4.89에서 8주 후 63.5±4.05kg로 1.3kg(2.01%), %fat는 35.4±2.57%에서 30.6±5.36%로 4.8%(13.56%)가 감소하였다. 체전굴은 9.4±5.44cm에서 8주후 9.7±5.63cm로 0.3cm당은 198.3±89.23mg/dl에서 8주 후 113.0±51.53mg/dl로 85.2mg/dl(43.00%), 인슐린은 12.1±3.38μU/ml에서 8주 후 9.8±3.69μU/ml로 2.30μU/ml(19.01%)가 감소하였다. Fructosamine(umol/L)은 339.5±79.70μmol/L에서 8주 후 259.8±54.0μmol/L로 79.75umol/L(23.49%)가 감소하였으며, Glucagon은 252.7±59.73mg/dl에서 287.1±79.16mg/dl로 34.4mg/dl(13.61%)가 증가하였다. T-C 는 0.85%, LDL-C은 0.56% 감소하였으며, HDL-C은 48.0±4.55mg/dl에서 8주 후 49.5±8.70mg/dl으로 1.5mg/dl(3.13%)가 증가하였다. 이상과 같이 HRmax 50^의 지속적이고 규칙적인 유산소 운동은 NIDDM 환자의 체지방량과 혈당을 감소시키고, 인슐린감수성과 HDL-C이 증가하여 당뇨병개선에 효과가 있는 것으로 나타났다.