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Cho, Eun Sook,Jin, Byung Rae,Sohn, Hung Dae,Choi, Kwang Ho,Kim, Soung Ryul,Kang, Seok Woo,Yun, Eun Young,Kim, Sang Hyun,Kim, Keun Young,Je, Yeon Ho,Kang, Seok Kwon 한국잠사학회 1998 한국잠사곤충학회지 Vol.40 No.2
To construct transfurmed Bm5 cells, Autographa californica nuclear polyhedrosis virus(AcNPV) IE1 gene, an immediate early viral gene was firstly used in this study. AcNPV IE1 gene, which shares on 95.3% nucleotide sequence homology with Bombyx mori nuclear polyhedrosis virus (BmNPV) IE1 gene, was isolated and cloned into pBluescript. Neomycin gene from pKO-neo was inserted under the control of the IE1 promoter to yield pAcIE1-neo. The plasmid pAcIE1-neo was transfected into Bm5 or Sf9 cells, and neomycin-resistant cells were selected in TC100 medium containing 10% fetal bovine serum (FBS) and 1 mg/ml G4l8 for two weeks. Individual clones were picked and each was amplified for further characterization. The genomic DNA from neomycin-resistant cells was isolated and characterized by PCR using AcNPV IEI gene-specific primers and by Southern blot analysis using neomycin gene probe. We concluded that AcNPV IE1 gene was functional in B. mori-derived Bm5 cells as well as Spodoptera fugjprrda-derived Sf9 cells to produce stably-transformed insect cells
Sung-Woo Park,Jin-Rae Cho 국제구조공학회 2012 Structural Engineering and Mechanics, An Int'l Jou Vol.41 No.4
An adaptive modeling and simulation technique is introduced for the effective and reliable fluid-structure interaction analysis using MSC/Dytran for large-scale complex pressurized liquid containment. The proposed method is composed of a series of the global rigid sloshing analysis and the locally detailed fluid-structure analysis. The critical time at which the system exhibits the severe liquid sloshing response is sought through the former analysis, while the fluid-structure interaction in the local region of interest at the critical time is analyzed by the latter analysis. Differing from the global coarse model, the local fine model considers not only the complex geometry and flexibility of structure but the effect of internal pressure. The locally detailed FSI problem is solved in terms of multi-material volume fractions and the flow and pressure fields obtained by the global analysis at the critical time are specified as the initial conditions. An in-house program for mapping the global analysis results onto the fine-scale local FSI model is developed. The validity and effectiveness of the proposed method are verified through an illustrative numerical experiment.
Cho, Il-Rae,Koh, Sang-Seok,Min, Hye-Jin,Kim, Su-Jin,Lee, Yang-Soon,Park, Eun-Hee,Ratakorn, Srisuttee,Jhun, Byung-Hak,Oh, Sang-Taek,Johnston, Randal N.,Chung, Young-Hwa Korean Society for Biochemistry and Molecular Bion 2011 Experimental and molecular medicine Vol.43 No.2
It is not yet understood how the enhanced expression of pancreatic adenocarcinoma up-regulated factor (PAUF; a novel oncogene identified in our recent studies), contributes to the oncogenesis of pancreatic cells. We herein report that PAUF up-regulates the expression and transcriptional activity of ${\beta}$-catenin while the suppression of PAUF by shRNA down-regulates ${\beta}$-catenin. The induction of ${\beta}$-catenin by PAUF is mediated by the activities of Akt and GSK-$3{\beta}$, but inhibition of downstream ERK does not reduce ${\beta}$-catenin expression. To test whether PAUF emulates either the Wnt3a-mediated or the protein kinase A-mediated signaling pathway for the stabilization of ${\beta}$-catenin, we examined the phosphorylation status of ${\beta}$-catenin in the presence of PAUF compared with that of ${\beta}$-catenin during treatment with Wnt3a or dibutyryl cAMP, a cell permeable cyclic AMP analogue. PAUF expression induces phosphorylation at Ser-33/37/Thr-41 and Ser-675 of ${\beta}$-catenin but no phosphorylation at Ser-45, indicating that a unique phosphorylation pattern of ${\beta}$-catenin is caused by PAUF. Finally, the expression of PAUF up-regulates both $cyclin-D1$ and $c-Jun$, target genes of ${\beta}$-catenin, leading to a rapid proliferation of pancreatic cells; conversely decreased PAUF expression (by shRNA) results in the reduced proliferation of pancreatic cells. Treatment with hexachlorophene (an inhibitor of ${\beta}$-catenin) reduces the proliferation of pancreatic cells despite the presence of PAUF. Taken together, we propose that PAUF can up-regulate and stabilize ${\beta}$-catenin $via$ a novel pattern of phosphorylation, thereby contributing to the rapid proliferation of pancreatic cancer cells.
Sung Gil Kim,Hee Rae Lim,Kang Hee Cho,Pue Hee Park,Su Hyung Park,Soon Kee Sung,Dae Geun Oh,Ki Taek Kim 한국육종학회 2009 한국육종학회지 Vol.41 No.3
Cytoplasmic male sterility (CMS) and fertility restoration have been utilized as valuable tools for F_1-hybrid seed production in many crops despite laborious breeding processes. Molecular markers for the selection of CMS-related genes help reduce the expenses and breeding times. A previously reported genomic region containing the Ppr-B gene, which is responsible for restoration of fertility and corresponds to the Rfo locus, was used to develop gene-based or so-called "functional" markers for allelic selection of the restorer-of-fertility gene (Rfo) in F_1-hybrid breeding of radish (Raphanus sativus L.) Polymorphic sequences among Rfo alleles of diverse breeding lines of radish were examined by sequencing the Ppr-B alleles. However, presence of Ppr-B homolog, designated as Ppr-D, interferes on specific PCR amplification of Ppr-B in certain breeding lines. The organization of Ppr-D, resolved by genome walking, revealed extended homology with Ppr-B even in the promoter region. Interestingly, PCR amplification of Ppr-D was repeatedly unsuccessful in certain breeding lines implying the lack of Ppr-D in these radishes. Ppr-B could only be successfully amplified for analysis through designing primers based on the sequences unique to Ppr-B that exclude interference from Ppr-D gene. Four variants of Rfo alleles were identified from 20 breeding lines. A combination of three molecular markers was developed in order to genotype the Rfo locus based on polymorphisms among four different variants. These markers will be useful in facilitating F_1-hybrid cultivar development in radish.
Effect of weld geometry on fatigue performance of 6061-T6 aluminum GMAW: part 2. Lap joint
Sang-Woo Han,Geonho Lee,Hansol Kim,Min-Soo Kim,Young-Rae Jo,Jung Ho Cho 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.10
To investigate the effect of weld geometry on the fatigue performance of aluminum lap joint arc welding, GMAW experiments and fatigue tests were conducted. The results show that the combination of a smaller toe angle and full penetration with a proper gap size results in the best fatigue performance. A proper gap size can disperse the stress concentration, and the dispersion increases fatigue performance. Because of the reduction in the welded area, partial penetration has a lower fatigue performance than the full-penetration case. In the lap joint, a large toe angle causes a smaller weld reinforcement owing to geometric reasons, and therefore exhibits a lower fatigue performance.
Aqueous Polymerization of Acrylamide Initiated by Periodic Acid and Its Kinetics
Cho, Myung-Rae,Han, Yang-Kyoo,Kim, Bum-Sung The Polymer Society of Korea 2000 Korea polymer journal Vol.8 No.4
The activity of periodic acid as an initiator for the polymerization of acrylamide in aqueous medium was investigated. The rate of polymerization was found to be proportional to the monomer concentration to the 1.5th power in the range of 1.41-5.64 mol/L. The reaction order to the periodic acid concentration was 0.49, which indicated a bimolecular mechanism for the termination reaction in the range of 0.5-4.0$\times$10$\^$-2/ mol/L. Propagation rate increased with raising the temperature according to an Arrhenius expression resulting in the exhibition of an apparent activation energy of 87.8 kJ/mol in the temperature range of 60-80$\^{C}$. The addition of hydroquinone as a radical scavenger stopped the polymerization of acrylamide initiated by periodic acid. These results support that the polymerization proceeds via a radical chain mechanism .
Effect of weld geometry on fatigue performance of 6061-T6 aluminum GMAW: part 3. T-fillet joint
Sang-Woo Han,Geonho Lee,Hansol Kim,Min-Soo Kim,Young-Rae Jo,Jung Ho Cho 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.10
The effects of various weld geometries on the fatigue performance of T-fillet joint aluminum arc welding were investigated by conducting gas metal arc weld (GMAW) experiments using 6061-T6 material for various weld geometries. The results of the fatigue tests conducted using a 5-ton grade tester demonstrated that the fatigue performance of T-fillet joint aluminum arc welding is primarily affected by the area of the welded part and weld reinforcement. A large welded area can be obtained by full penetration, and a large weld reinforcement can be obtained by a large throat size and a small toe angle. Contrary to expectations, it was observed that optimal size and higher quantity of porosities can increase fatigue performance. This can be attributed to porosities behaving similarly to crack stop holes, which can prevent stress focusing. The deviation in fatigue performance in the case of minute penetration depth and minute throat size is not remarkable. Therefore, deeper penetration and larger weld reinforcement are crucial factors in obtaining higher fatigue performance in the industry.