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Chun, Yoon-Keun,Ha, Joo-hun,Hong-Jung-Woo,Oh, Soo-Myung,Kim, Sung-Soo 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1
Yoon-Keun Chun¹,Joohun Ha□Hong-Jung Woo□, Soo Myung Oh□,Sung Soo Kim□ ¹Department of Molecular Biology, College of Medicine,²Department of Surgery, college of Medicine,³Department of Internal Medicine, College of Oriental Medicine,and ⁴East-Weat Medical Reserch Institute,Kyung Hee University, Seoul, Korea. The HBV DNA Amounts in Serum Have No relationship with ALT level and Hetergeneous Population Coexits in Chronic Hepatitis B Virus Infection. Proceedings of International Symposium on East-West Medicine, Seoul. 212-230, 1999. -Hepatitis B is caused by hepadnavirus. Hepatitis B virus replicates through 3.5kb pregenomic RNA intermediate which is regulated by core promoter. Pathogenesis of hepatitis B virus has been bilieved the result of host immune response. But recently many studies have reported that high level of viral replication caused by mutation in core promoter might result in severs hepatitis. But these studies were performed in vitro, not in vivo. So there is yet debate about which factor, viral of host factor, is more important in pathogenesis of hepatitis B virus. So we measured real viral replication level in 204 chronic hepatitis B patients by quantifying HBV DNA from sera by our novel PCR-based more sensitive method, and compared these results with ALT level measured from same sera, which indicates liver cell damage. Surprisingly there are no significant correlation between HBV DNA quantity and ALT level. Then we cloned core promoter region. In SSCP, we found that many viral mutants coexist in one patient. Base on SSCP result, we chose main viral core promoter type in each patients, which is thought to determine overall viral replication level in this patient. Main type of core promoter region of each 41 patients were directly sequenced. And with these we measured promoter activity by luciferase assay system and compared promoter activity with on another. We found tha there were some differences in promoter activity according to core promoter sequences. And we constructed replication-competent viral constructs with core promoter from 41 patients and Transfected these into HepG2 cell and measured HBV DNA by southern blot. There were also differences in HBV DNA quantity according to core promoter sequences. On these all results we investigated correlation between the effect of HBV core promoter on viral replication in vitro and HBN DNA quantity, ALT level from sera of each patients. We found there is no significant correlation among them. As a result, we concluded that in determining severity chronic hepatitis B patients, host factors of each patient is more important rather than replicative activity of virus itself.
중금속 오염 논토양에서 카드뮴의 부동화와 식물이용성에 대한 석회 시용 효과
홍창오 ( Chang Oh Hong ),김용균 ( Yong Gyun Kim ),이상몽 ( Sang Mong Lee ),박현철 ( Hyean Cheal Park ),김근기 ( Keun Ki Kim ),손홍주 ( Hong Joo Son ),조재환 ( Jae Hwan Cho ),김필주 ( Pil Joo Kim ) 한국환경농학회 2013 한국환경농학회지 Vol.32 No.1
Research Article : BACKGROUND : Many studies associated with cadmium (Cd) immobilization using lime fertilizer have been conducted for several decades. However, these studies did not suggest exact mechanism of Cd immobilization using lime fertilizer and evaluated effect of lime fertilizer on Cd phytoavailability in rice paddy soil under field condition. METHODS AND RESULTS: This study was conducted to determine exact mechanism of Cd immobilization using lime fertilizer and evaluate liming effect on Cd uptake of rice in contaminated paddy soil. Ca(OH)2 was mixed with Cd contaminated arable soil at rates corresponding to 0, 1,000, 2,000, 4,000, and 8,000 mg/kg. The limed soil was moistened to paddy soil condition, and incubated at 25℃ for 4 weeks. NH4OAc extractable Cd concentration in soil decreased significantly with increasing Ca(OH)2 rate, since Ca(OH)2 markedly increased net negative charge of soil by pH increase, and decreased bioavailable Cd fractions (F1; exchangeable + acidic and reducible Cd fraction). Calculated solubility diagram indicated that Cd solubility was controlled by soil-Cd. NH4OAc extractable Cd and F1 concentration were negatively related to soil pH and negative charge. Ca(OH)2 was applied at rates 0, 2, 4, and 8 Mg/ha and then cultivated rice in the paddy soil under field condition. Cadmium concentrations in grain, straw, and root of rice plant decreased significantly with increasing application rate of Ca(OH)2. CONCLUSION(S): Alleviation of Cd phytoavailability with Ca(OH)2 can be attributed primarily to Cd immobilization due to the increase in soil pH and negative charge rather than precipitation of Cd(OH)2 or CdCO3, and therefore, Ca(OH)2 is effective for reducing Cd phytoavailability of rice in paddy soil.
Sang-Hyun Oh,Eui-Seong Hwang,Eun-Seok Choi,Gyu-Dong Park,Jin-Gu Kim,Jin-Yong Seong,Jun-Hee Cho,Keun-Do Ban,Keun-Hwan Noh,Nam-Kyeong Kim,Seaung-Suk Lee,Seok-Won Lee,Seung-Jin Yeom,Soon-Yong Kweon,Suk-K 한국물리학회 2003 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.42 No.IV
We have investigated the ferroelectric properties of integrated Pt/SrBi2Ta2O9 (SBT)/Pt capacitors with capacitor-level dielectric of boron phosphosilicate glass (BPSG). A signicant reduction of remanent polarization (P-P^) was observed in the SBT capacitor as covered with BPSG lm and the polarization decay depended strongly on top electorde size. Detailed analyses showed the degradation resulted from bismuth loss in the surface region of SBT, which is closely related with the impurites diused from BPSG into SBT layer. The degradation of Pt/SBT/Pt ferroelectric capacitor was successfully prevented by inserting SiO2 layer as a impurity blocking layer between the SBT and the BPSG. Thereby device performances of SBT-based ferroelectric memory were also considerably improved.
Oh, Sung Aeong,Park, Keun Sang,Twell, David,Park, Soon Ki Blackwell Publishing Ltd 2010 The Plant journal Vol.64 No.5
<P><B>Summary</B></P><P>Cellular patterning and differentiation in plants depend on the balance of asymmetric and symmetric divisions. Patterning of the male gametophyte (pollen grains) in flowering plants requires asymmetric division of the microspore followed by a symmetric division of the germ cell to produce three highly differentiated cells: a single vegetative cell and two sperm cells. In Arabidopsis <I>sidecar pollen</I> (<I>scp</I>) mutants a proportion of microspores first divide symmetrically, and then go on to produce ‘four‐celled’ pollen with an extra vegetative cell; however, details of the timing and origin of phenotypic defects in <I>scp</I> and the identity of the <I>SCP</I> gene have remained obscure. Comparative analysis of the original hypomorphic <I>scp‐1</I> allele and a T‐DNA‐induced null allele, <I>scp‐2</I>, revealed that in the absence of SCP, microspores undergo normal nuclear positioning, but show delayed entry into mitosis, increased cell expansion and alterations in the orientation of nuclear division. We identified the <I>SCP</I> gene to encode a male gametophyte‐specific LATERAL ORGAN BOUNDARIES DOMAIN/ASYMMETRIC LEAVES 2‐like (LBD/ASL) protein that is expressed in microspore nuclei in a tightly regulated phase‐specific manner. Therefore, our study demonstrates that the correct patterning of male gametophyte depends on the activity of a nuclear LBD/ASL family protein that is essential for the correct timing and orientation of asymmetric microspore division.</P>
Neural Interface with a Silicon Neural Probe in the Advancement of Microtechnology
Oh, Seung-Jae,Song, Jong-Keun,Kim, Sung-June The Korean Society for Biotechnology and Bioengine 2003 Biotechnology and Bioprocess Engineering Vol.8 No.4
In this paper we describe the status of a silicon-based microelectrode for neural recording and an advanced neural interface. We have developed a silicon neural probe, using a combination of plasma and wet etching techniques. This process enables the probe thickness to be controlled precisely. To enhance the CMOS compatibility in the fabrication process, we investigated the feasibility of the site material of the doped polycrystalline silicon with small grains of around 50 nm in size. This silicon electrode demonstrated a favorable performance with respect to impedance spectra, surface topography and acute neural recording. These results showed that the silicon neural probe can be used as an advanced microelectrode for neurological applications.
Oh, Sang-Keun,Kim, Saet-Byul,Yeom, Seon-In,Lee, Hyun-Ah,Choi, Do-Il Korean Society for Molecular and Cellular Biology 2010 Molecules and cells Vol.30 No.6
Transient expression is an easy, rapid and powerful technique for producing proteins of interest in plants. Recombinational cloning is highly efficient but has disadvantages, including complicated, time consuming cloning procedures and expensive enzymes for large-scale gene cloning. To overcome these limitations, we developed new ligation-independent cloning (LIC) vectors derived from binary vectors including tobacco mosaic virus (pJL-TRBO), potato virus X (pGR106) and the pBI121 vector-based pMBP1. LIC vectors were modified to enable directional cloning of PCR products without restriction enzyme digestion or ligation reactions. In addition, the ccdB gene, which encodes a potent cell-killing protein, was introduced between the two LIC adapter sites in the pJL-LIC, pGR-LIC, and pMBP-LIC vectors for the efficient selection of recombinant clones. This new vector does not require restriction enzymes, alkaline phosphatase, or DNA ligase for cloning. To clone, the three LIC vectors are digested with SnaBI and treated with T4 DNA polymerase, which includes 3' to 5' exonuclease activity in the presence of only one dNTP (dGTP for the inserts and dCTP for the vector). To make recombinants, the vector plasmid and the insert PCR fragment were annealed at room temperature for 20 min prior to transformation into the host. Bacterial transformation was accomplished with 100% efficiency. To validate the new LIC vector systems, we were used to coexpressed the Phytophthora AVR and potato resistance (R) genes in N. benthamiana by infiltration of Agrobacterium. Coexpressed AVR and R genes in N. benthamiana induced the typical hypersensitive cell death resulting from in vivo interaction of the two proteins. These LIC vectors could be efficiently used for high-throughput cloning and laboratory-scale in planta expression. These vectors could provide a powerful tool for high-throughput transient expression assays for functional genomic studies in plants.
Oh, Sang-Keun,Lee, Seon-Woo,Kwon, Suk-Yoon,Choi, Do-Il The Korean Society of Plant Pathology 2005 Plant Pathology Journal Vol.21 No.3
Pathogenesis-related protein-1a (PR-1a) is strongly induced in tobacco plants by pathogen attack, exogenous salicylic acid (SA) application and by other developmental processes. In order to develop a rapid screening system for the selection of plant defense-inducing compounds originated from various sources, we have transformed tobacco Samsun NN plants with a chimeric construct consisting of GUS $(\beta-glucuronidase)$. In the $T_1$ generation, three transgenic lines having stable GUS expression were selected for further promoter analysis. Using GUS histochemical assay, we observed strong GUS induction driven by PR-1a promoter in PR1a-GUS transgenic tobacco leaves in response to the exogenous application of SA or benzol (1,2,3) thiadiazole-7-carbothioic acid S-methyl ester (BTH), a SAderivative compound. In addition, GUS expression was maintained locally or systemically in PR1a-GUS transgenic line $\#5\;T_2$ generation) until after 3 days when they were treated with same chemicals. Our results suggested that the PR1a-GUS reporter gene system in tobacco plants may be applicable for the large-scale screening of defense-inducing substances.
Oh, Jisun,Kim, Jungeun,Jang, Jin Ho,Lee, Sangwoo,Park, Chul Min,Kim, Woo-Keun,Kim, Jong-Sang MDPI 2018 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.19 No.4
<P>The present study aimed to evaluate the anti-melanogenic activity of 1,6-diphenyl-1,3,5-hexatriene and its derivatives in B16F10 murine melanoma cells and zebrafish embryos. Twenty five (1<I>E</I>,3<I>E</I>,5<I>E</I>)-1,6-<I>bis</I>(substituted phenyl)hexa-1,3,5-triene analogs were synthesized and their non-cytotoxic effects were predictively analyzed using three-dimensional quantitative structure-activity relationship approach. Inhibitory activities of these synthetic compounds against melanin synthesis were determined by evaluating melanin content and melanogenic regulatory enzyme expression in B16F10 cells. The anti-melanogenic activity was verified by observing body pigmentation in zebrafishes treated with these compounds. Compound <B>#2</B>, <B>#4</B>, and <B>#6</B> effectively decreased melanogenesis induced by α-melanocyte-stimulating hormone. In particular, compound <B>#2</B> remarkably lowered the mRNA and protein expression levels of microphthalmia-associated transcription factor (MITF), tyrosinase (TYR), tyrosinase-related protein 1 (TYRP1), and TYRP2 in B16F10 cells and substantially reduced skin pigmentation in the developed larvae of zebrafish. These findings suggest that compound <B>#2</B> may be used as an anti-melanogenic agent for cosmetic purpose.</P>