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RISS 인기검색어
- 검색키워드
- 저자 : Samell Keo (검색결과 5 건)
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Neuroprotective Effects of Cambodian Plant Extracts on Glutamate-induced Cytotoxicity in HT22 Cells
Samell Keo,이동성,Bin Li,Hyun-Gyu Choi,Kyoung-Su Kim,Wonmin Ko,오현철,김윤철 한국생약학회 2012 Natural Product Sciences Vol.18 No.3
Oxidative stress potentially induces neurotoxicity which is believed to underlie several major age-related diseases of the central nervous system. This study sought to identify the cytoprotective effects of sixty-nine Cambodian plants against glutamate-induced cell death. Cultured HT22 cells were applied as an in vitro model, and neurotoxicity was induced in these neuronal cells by exposure to a determined concentration of glutamate. Sixty-nine plant sources, as Cambodia's indigenous species, were purchased from O'reusey Market, Phnom Penh, and extracted with ethanol. These extracts were screened for cytoprotective effects against glutamate-triggered neurotoxicity in HT22 cells at concentrations of 100 and 300 ?g/ml. Of these, eight ethanol extracts, bark of Anacardium occidentale, bark and sapwood of Bauhinia pulla, flowers of Borassus flabellifer, stems and leaves of Coix lacryma-jobi, bark and sapwood of Diospyros nitida, sapwood of Dipterocarpus obtusifolius, stems of Oryza rufipogon, and fruits of Phyllanthus emblica, showed significant cytoprotective effects against glutamate-induced cell damage and degeneration in HT22 cells.
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이동성,Samell Keo,고원민,김경수,Elena Ivanova,임정한,김연철,오현철 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.7
8-Epiloganin (1), mussaenoside (2), and5-O-caffeoylshikimic acid (3) have been isolated fromCastilleja rubra, and the anti-inflammatory properties ofthese metabolites in a cell culture system were investigated. Compounds 1–3 suppressed not only the productionof nitric oxide (NO) and prostaglandin E2, butalso the expression of inducible NO synthase andcyclooxygenase-2 induced by lipopolysaccharide (LPS)in the RAW264.7 murine macrophage cell line. Compounds1–3 also inhibited the release of pro-inflammatorycytokines induced by LPS, namely, tumor necrosisfactor-a and interleukin-1b. The underlying mechanismof the anti-inflammatory action of compounds 1–3 wasassociated with downregulation of nuclear factor-jB.
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Lee, Dong-Sung,Keo, Samell,Ko, Wonmin,Kim, Kyoung-Su,Ivanova, Elena,Yim, Joung Han,Kim, Youn-Chul,Oh, Hyuncheol 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.7
8-Epiloganin (1), mussaenoside (2), and 5-O-caffeoylshikimic acid (3) have been isolated from Castilleja rubra, and the anti-inflammatory properties of these metabolites in a cell culture system were investigated. Compounds 1-3 suppressed not only the production of nitric oxide (NO) and prostaglandin $E_2$, but also the expression of inducible NO synthase and cyclooxygenase-2 induced by lipopolysaccharide (LPS) in the RAW264.7 murine macrophage cell line. Compounds 1-3 also inhibited the release of pro-inflammatory cytokines induced by LPS, namely, tumor necrosis factor-${\alpha}$ and interleukin-$1{\beta}$. The underlying mechanism of the anti-inflammatory action of compounds 1-3 was associated with downregulation of nuclear factor-${\kappa}B$.
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이동성,오현철,김경수,Samell Keo,정길생,김윤철 한국생약학회 2013 Natural Product Sciences Vol.19 No.1
Rhus verniciflua Stokes (Anacadiaceae) is a plant that is native to East Asian countries, such as Korea, China, and Japan, and it has been found to exert various biological activities including antioxidative, anti-aggregatory, anti-inflammatory, anti-mutagenic, and apoptotic effects. Sulfuretin is one of the major flavonoid component isolated from the heartwood of R. verniciflua. Reactive oxygen species (ROS), produced via dental adhesive bleaching agents and pulpal disease, can cause oxidative stress. In the present study, we isolated sulfuretin from R. verniciflua and demonstrated that sulfuretin possesses cytoprotective effects against hydrogen peroxide (H2O2)-induced dental cell death. H2O2 is a representative ROS and causes cell death through necrosis in human dental pulp (HDP) cells. H2O2-induced cytotoxicity and production of ROS were blocked in the presence of sulfuretin, and these effects were dose dependent. Sulfuretin also increased heme oxygenase-1 (HO-1) protein expression. In addition, to determine whether sulfuretin-induced HO-1 expression mediated this cytoprotective effect, HDP cells were cotreated with sulfuretin in the absence or presence of SnPP, an inhibitor of HO activity. Sulfuretin-dependent HO-1 expression was required for suppression of H2O2-induced HDP cell death and ROS generation. These results indicate that sulfuretin-dependent HO-1 expression was required for the inhibition of H2O2-induced cell death and ROS generation. In addition, sulfuretin may be used to prevent functional dental cell death and thus may be useful as a pulpal disease agent.
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Lee, Dong-Sung,Keo, Samell,Cheng, Sun-Kaing,Oh, Hyuncheol,Kim, Youn-Chul SPANDIDOS PUBLICATIONS 2017 MOLECULAR MEDICINE REPORTS Vol. No.
<P>Liver diseases are considered to be primary contributors to morbidity and mortality rates in humans. Oxidative stress is critical in liver injury, and oxidant-induced liver injury may be caused by toxins, including tert-butyl hydroperoxide (t-BHP). The present study investigated the hepatoprotective activities of 64 crude ethanol extracts of Cambodian medicinal plants against t-BHP-induced cytotoxicity in human liver-derived HepG2 cells, and assessed their cytoprotective mechanism pertaining to the expression of heme oxygenase (HO)-1 and nuclear factor E2-related factor 2 (Nrf2). Protective effects in HepG2 cells were determined by MTT assay. Protein expression levels of HO-1 and Nrf2 were determined by western blotting and mRNA expression levels were determined by reverse transcription-quantitative polymerase chain reaction. Of the 64 extracts, 19 extracts exhibited high hepatoprotective activities: Ampelocissus martini, Bauhinia bracteata, Bombax ceiba, Borassus flabellifer, Cardiospermum halicacabum, Cayratia trifolia, Cinnamomurn caryophyllus, Cyperus rotundus, Dasymaschalon lomentaceum, Ficus benjamina, Mangifera duperreana, Morinda citrifolia, Pandanus humilis, Peliosanthes weberi, Phyllanthus emblica, Quisqualis indica, Smilax glabra, Tinospora crispa and Willughbeia cochinchinensis, with half maximal effective concentrations ranging between 59.23 and 157.80 mu g/ml. Further investigations revealed that, of these 19 extracts, HO-1 and Nrf2 were expressed in P. weberi and T. crispa expressed in a dose-dependent manner. In addition, the activities of reactive oxygen species were suppressed following treatment of these</P>
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