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Aeration Effects on Metabolic Events during Sporulation of Bacillus thuringiensis
Mohammad H. Sarrafzadeh,Sabine Schorr-Galindo,Hyun-Joon La,오희목 한국미생물학회 2014 The journal of microbiology Vol.52 No.7
The metabolism of Bacillus thuringiensis during its sporulation process was investigated under different concentrations of oxygen. At the beginning of sporulation, the aeration conditions were regulated to obtain different oxygentransfer rates (OTR) in four separate fermentations, representing interrupted, limited, non-limited, and saturated oxygenation, respectively. A higher OTR resulted in a higher pH, up to about 9 in the case of saturated oxygenation,while the interrupted oxygenation resulted in a significantly acidic culture. In contrast, the absence of oxygen resultedin rapid sporangia lysis and caused acidification of the medium, indicating a distinctly different sporangia composition and different metabolism. The bacterium also showed different CO2 production rates during sporulation, although a maximum point was observed in every case. With a higher OTR, the maximal value was observed after a longer time and at a lower value (40, 26, and 13 mmol/L/h for limited, non-limited, and saturated cases, respectively). Despite the exhaustion of glucose prior to the sporulation phase, the interrupted oxygenation resulted in acetate, lactate, and citrate in the medium with a maximum concentration of 4.8,1.3, and 5.0 g/L, respectively. Notwithstanding, while themetabolic events differed visibly in the absence of oxygen,once sporulation was triggered, it was completed, even in the case of an interrupted oxygen supply.