http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Sunghyun Kim,Jang-Eun Cho,Hyunjung Kim,Dongsup Lee,Bo-young Jeon,Hyejon Lee,Sang-Nae Cho,Young Keun Kim,Hyeyoung Lee 대한의생명과학회 2013 Biomedical Science Letters Vol.19 No.2
The tuberculin skin test (TST) and interferon gamma (IFN-γ) release assay (IGRA) have been widely used for diagnosis of latent tuberculosis infection (LTBI). In order to overcome limitations of current LTBI diagnostic methods, the development of a novel molecular assay which is able to measure the IFN-γ messenger RNA (mRNA) expression level after stimulation with Mycobacterium tuberculosis (MTB) specific antigen was recently developed. The ability of a molecular assay to detect MTB infection was similar to commercial IGRA however, the optimal incubation time for stimulating IFN-γ was not yet established. Therefore, in this study the direct comparisons of MTB Ag stimulation times (4 and 24 hrs) were performed for diagnosis of MTB infection. Data showed that the coincident rate between QFT-GIT IFN-γ ELISA and IFN-γ RT-PCR (4 hrs) was 88.35% and that of QFT-GIT and IFN-γ RT-PCR (24 hrs) was 70.85%. Based on a receiver operating characteristic (ROC) curve, the 4 hrs-MTB specific Ag stimulation time for IFN-γ RT-PCR had the significant P value, 95% CI value, and AUC (P < 0.0001, 95% CI=0.82 to 1.02, and AUC=0.9214) in comparison with 24 hrs-MTB specific Ag stimulation time (P = 0.009, 95% CI=0.06 to 0.94, and AUC=0.7711). These results show that 4-hr was the most optimal MTB Ag stimulation time for performing IFN-γ RT-PCR. Although semi-quantitative RT-PCR had a few analytical limitations, it might be useful as an alternative molecular diagnostic method for detecting MTB infection.
Kim, Sang Hee,Kim, Yongmo,Kim, Moonil,Kim, Dae Shick,Lee, Sang Chul,Chi, Seung-Wook,Lee, Do Hee,Park, Sung Goo,Park, Byoung Chul,Bae, Kwang-Hee,Kang, Sunghyun Pergamon Press 2009 Oncology Research Vol.17 No.11
<P>Metastasis is a complex, multistep process by which a cancer cell leaves the primary tumor, travels to a distant site via the circulatory system, and establishes a secondary cancer. A deeper understanding of the molecular events underlying metastasis will provide information that will be useful for the development of new diagnostic and therapeutic strategies. The B16 and B16F10 mouse melanoma cell lines are widely used as model system for studying many aspects of cancer biology including metastasis. Compared with B16, which has a low metastatic potential, the highly metastatic cell line B16F10 displayed a higher metastatic ability along with higher expression levels of the metastasis-associated phosphatase of regenerating liver-3 (PRL-3). B16 cells transfected with PRL-3 cDNA (B16-PRL3) had metastatic abilities comparable to those of Bl16F10 cells. To study the molecular mechanisms that underlie metastasis, the proteomes of the B16, B16F10, and B16-PRL3 cell lines were compared using two-dimensional differential in-gel electrophoresis. Proteins that varied significantly in levels between these cell lines were selected and identified using mass spectrometry. Interestingly, many proteins, especially those present in membrane fractions, were similarly up- or downregulated in both the Bl16F10 and B16-PRL3 cells lines compared to B16 cell lines. The list of similarly regulated proteins included heat shock protein 70, fascin-1, septin-6, ATP synthase beta subunit, and bone morphogenic protein receptor type IB. These proteins may play a causal role in PRL-3-mediated metastasis. These investigations open an avenue for the further characterization of the molecular mechanisms that underlie metastasis.</P>
Kim, Sunghyun,Park, Jeong Won,Kim, Dongkyu,Kim, Daejin,Lee, In-Hyun,Jon, Sangyong WILEY-VCH Verlag 2009 Angewandte Chemie Vol.121 No.23
<P>Zuviel Calcium im Blut? Mit Calsequestrin (CSQ) funktionalisierte Goldnanopartikel durchlaufen eine Calcium-abhängige CSQ-Polymerisation, die mit einem Farbwechsel (siehe Bild) und einer Niederschlagsbildung einhergeht. Dieses Sensorsystem ist spezifisch für Ca<SUP>2+</SUP>-Ionen. Die Unterschiede zwischen normalen und krankheitsbedingten (hypercalcämischen) Ca<SUP>2+</SUP>-Ionenkonzentrationen im Serum lassen sich mit bloßem Auge wahrnehmen. <img src='wiley_img/00448249-2009-121-23-ANGE200900071-content.gif' alt='wiley_img/00448249-2009-121-23-ANGE200900071-content'> </P>
Genome-wide target specificities of CRISPR-Cas9 nucleases revealed by multiplex Digenome-seq
Kim, Daesik,Kim, Sojung,Kim, Sunghyun,Park, Jeongbin,Kim, Jin-Soo Cold Spring Harbor Laboratory Press 2016 Genome Research Vol.26 No.3
<P>We present multiplex Digenome-seq to profile genome-wide specificities of up to 11 CRISPR-Cas9 nucleases simultaneously, saving time and reducing cost. Cell-free human genomic DNA was digested using multiple sgRNAs combined with the Cas9 protein and then subjected to whole-genome sequencing. In vitro cleavage patterns, characteristic of on- and off-target sites, were computationally identified across the genome using a new DNA cleavage scoring system. We found that many false positive, bulge-type off-target sites were cleaved by sgRNAs transcribed from an oligonucleotide duplex but not by those transcribed from a plasmid template. Multiplex Digenome-seq captured many bona fide off-target sites, missed by other genome-wide methods, at which indels were induced at frequencies <0.1%. After analyzing 964 sites cleaved in vitro by these sgRNAs and measuring indel frequencies at hundreds of off-target sites in cells, we propose a guideline for the choice of target sites for minimizing CRISPR-Cas9 off-target effects in the human genome.</P>
Clinical Evaluation of Human Papillomavirus DNA Genotyping Assay to Diagnose Women Cervical Cancer
Sunghyun Kim,Dongsup Lee,Yeun Kim,Geehyuk Kim,Sangjung Park,Yeonim Choi,Tae Ue Kim,Kwang Hwa Park,Hyeyoung Lee 대한의생명과학회 2012 Biomedical Science Letters Vol.18 No.2
In this study, we evaluated the human papillomavirus (HPV) genotyping test called MolecuTech REBA HPV-ID® (YD Diagnostics, Seoul, Korea) for 704 women who also had cervical cytological evaluations by Thin Prep. The infection rate of high-risk HPV genotypes was 56.6% in patients with normal cytology, 59.8% in those with benign, low-grade squamous intraepithelial lesions, 51.4% in those with atypical squamous cells of uncertain significance, 92.3% in those with high-grade squamous intraepithelial lesions, and 94.1% in those with squamous cell carcinoma or adenocarcinoma. HPV 16 was the most common genotype detected in any lesion, followed by HPV 53, 58, 33, 52, 45, 31, and 35, in order. The HPV DNA test with PCR-REBA is a very highly sensitive, but less specific, method. The infection rates and HPV genotype distribution of non-Korean people versus people from South Korea showed regional differences.
Changes of Cytosolic Ca<SUP>2</SUP> under Metabolic Inhibition in Isolated Rat Ventricular Myocytes
Sunghyun Kang,Nari Kim,Hyun Joo,Jae Boum Youm,Won Sun Park,Mohamed Warda,Hyungkyu Kim,Dang Van Cuong,Taeho Kim,Euiyong Kim,Jin Han 대한생리학회-대한약리학회 2005 The Korean Journal of Physiology & Pharmacology Vol.9 No.5
To characterize cytosolic Ca<SUP>2</SUP> fluctuations under metabolic inhibition, rat ventricular myocytes were exposed to 200μM 2,4-dinitrophenol (DNP), and mitochondrial Ca<SUP>2</SUP>, mitochondrial membrane potential (ΔΨ<SUB>m</SUB>), and cytosolic Ca<SUP>2</SUP> were measured, using Rhod-2 AM, TMRE, and Fluo-4 AM fluorescent dyes, respectively, by Laser Scanning Confocal Microscopy (LSCM). Furthermore, the role of sarcolemmal Na<SUP></SUP>/Ca<SUP>2</SUP> exchange (NCX) in cytosolic Ca<SUP>2</SUP> efflux was studied in KB-R7943 and Na<SUP></SUP>-free normal Tyrode s solution (143 mM LiCl ). When DNP was applied to cells loaded with Fluo-4 AM, Fluo-4 AM fluorescence intensity initially increased by 70⁑10% within 70⁑10 s, and later by 400⁑200% at 850⁑46 s. Fluorescence intensity of both Rhod-2 AM and TMRE were initially decreased by DNP, coincident with the initial increase of Fluo-4 AM fluorescence intensity. When sarcoplasmic reticulum (SR) Ca<SUP>2</SUP> was depleted by 1μM thapsigargin plus 10μM ryanodine, the initial increase of Fluo-4 AM fluorescence intensity was unaffected, however, the subsequent progressive increase was abolished. KB-R7943 delayed both the first and the second phases of cytosolic Ca<SUP>2</SUP> overload, while Na<SUP></SUP>-free solution accelerated the second. The above results suggest that: 1) the initial rise in cytosolic Ca<SUP>2</SUP> under DNP results from mitochondrial depolarization; 2) the secondary increase is caused by progressive Ca<SUP>2</SUP> release from SR; 3) NCX plays an important role in transient cytosolic Ca<SUP>2</SUP> shifts under metabolic inhibition with DNP.