Red light-emitting diode irradiation regulates oxidative stress and inflammation through SPHK1/NF-κB activation in human keratinocytes

Sun, Qiaochu,Kim, Hye-Eun,Cho, Hyejoung,Shi, Shuhan,Kim, Byungkuk,Kim, Okjoon Elsevier 2018 Journal of photochemistry and photobiology Biology Vol.186 No.-

<P><B>Abstract</B></P> <P>Oxidative stress, in which the amount of oxidants exceeds the capacity of antioxidant defense system, is a well-accepted pathogenesis of several human diseases. Light-emitting diode irradiation (LEDI) is an efficient strategy to counteract this condition. The biological effect of phototherapy, using visible light, has attracted recent attention especially in dermatological practice. However, little is known about the molecular mechanism of the anti-oxidant and anti-inflammatory effects of red light irradiation. We evaluated these effects of LEDI in HaCaT human keratinocyte cells under phorbol-12-myristate-13-acetate (PMA) induced reactive oxygen species (ROS). Microarray analysis revealed changes in 309 genes after LEDI. LEDI at 625 nm produced ROS scavenging and anti-inflammatory effects. One of the most important genes identified by microarray analysis was sphingosine kinase-1 (SPHK1), which is a key molecule in sphingolipid metabolism. SPHK1 knock-down drastically reduced ROS scavenging efficiency as well as expression levels of inflammation-related proteins in PMA-treated HaCaT cells. These results not only indicate the potential for the clinical application of 625-nm LEDI in treating skin disorders via ROS and/or inflammation, but also suggest SPHK1 as a potential therapeutic target in phototherapy.</P> <P><B>Highlights</B></P> <P> <UL> <LI> LEDI at 625 nm produced ROS scavenging and anti-inflammation. </LI> <LI> Sphingosine kinase-1 (SPHK1) was identified by microarray analysis in the experimental conditions. </LI> <LI> LEDI 625 nm has the potential for treating skin disorder via ROS and/or inflammation through SPHK1/NF-κB pathway. </LI> </UL> </P> <P><B>Graphical Abstract</B></P> <P>[DISPLAY OMISSION]</P>

치약개발 경향 분석 - 조성물에 관하여 -

황동구,나리수,조혜중,김서진,김혜은,SUN QIAOCHU,지형준,안규현,최홍란,김옥준 대한구강악안면병리학회 2016 대한구강악안면병리학회지 Vol.40 No.1

Along with toothbrushes, toothpaste has been used for a long time to improve the personal oral hygiene. Toothpaste was firstly introduced to Korea through as a form of powder by the Japanese company Lion Corporation in 1889 and in 1954, the LakHee Chemistry company released Korea’ s first ever tube type cream toothpaste called lucky toothpaste, and afterwards we got to where we are today in terms of toothpaste. As a variety of products are released and customer choices become more diverse, there needs to be a development of toothpaste that can fulfill customers’ needs and requests. Therefore, this study analyzes toothpastes and related patents submitted to the Patent Office from 1962 to June 2015, thereby examining the changes in toothpaste development trends up to the present day. This study was searched using the keyword ‘toothpaste’ in the patent, utility model search engine at Kipris(www.kipris.or.kr), a patent search site, and categorized a total of 2,465 patents, which excluded those rejected by the Patent Office and those unrelated to toothpaste, using titles, full texts of publications and announcements. Research results showed that in the past, from 1960 to 1989, patents related to toothpaste containers took up the highest ratio of 35%. However, after 1990, patents pertaining to toothpaste composites and functionality made up the highest percentage of 54%, and from 2010 to 2015, it occupied 72% of the whole; therefore, we analyzed in detail the patents related to composites and functionality. Taking a look at the objectives and effects of patents having to do with composites and functionality, we found out that from 1964 to June 2015, patents related to Base, a basic component of toothpaste, and those having to do with periodontal disease took up the highest percentages of 25% each, and from 2010~2015 in particular, patents concerning periodontal diseases were submitted 3% more than those about Base. Composites aiming to prevent and treat periodontal diseases can be divided into four different categories, based on components; among those, there were a total of 171 (57%) patents related to living organism and herbal medicine extracts, 107 (36%) patents using compounds, 16 (5%) patents using bamboo salt as well as salt, and 5 (2%) patents using mineral components such as gold, silver. Looking at these investigation results, it can be thought that with the increasing development of toothpaste related to periodontal diseases, there’ s a great amount of interest about toothpaste of effective periodontal disease prevention and treatment purposes. Along with this, we thought that research about how effective periodontal disease toothpastes actually are is also necessary.

칫솔의 특허 경향 분석

최진성,이영희,조혜중,김서진,김혜은,SUN QIAOCHU,지형준,안규현,최홍란,김옥준 대한구강악안면병리학회 2016 대한구강악안면병리학회지 Vol.40 No.1

The basic instrument for oral hygiene is toothbrush. Many patents that are announced to enhance efficiency and convenience by changing shape and using new material. There are also patents regarding its various functions. This study, the purpose of study, was surveyed the toothbrush patents from 2005 to 2014. The patent search was done in site 'kipris' using keyword ‘toothbrush’. Among sorts of administrative measure, enrollment and extinction patents was searched, followed by classified patents into its shape, materials, function and electronic toothbrush. For the patents with function, detailed analysis was done. Total numbers of toothbrush patents decreased in 2006 to 2008, lowest in 2008, and steadily increased to 2014. The patents regarding shape was outnumbered material. Shape and material patents could be divided by handle portion and head portion patents, which patents of head portion is more than handle portion. The patents of function could be classified into certain situation or combinate functions to its natural function. Function patents increase with the ratio of total patents. For brush patents, they have a tendence of increase. In addition, from a shape and function, toothbrush patents going to be more variable. It choose small quantity batch production. From a shape of toothbrush, patents regarding head portion are going to increase. And it has a tendency to change the shape rather than the development of materials for efficiency of toothbrushing. Electronic toothbrush will be placed certain position, however, conventional toothbrush also will place large portion by estimating patent number and there be more progressive. Some patents will catch popularity like combination of brush and paste, replacement of brush head, attraction for toothbrushing, and hygiene tooth brush. Also disposable toothbrush and portable toothbrush are getting popularity along with social phenomenon.

Sialadenoma papilliferum에 대한 사례보고와 문헌고찰

김보현,HE YUZHU,SUN QIAOCHU,김병국,김옥수,김영,김옥준 대한구강악안면병리학회 2019 대한구강악안면병리학회지 Vol.43 No.4

Sialadenoma papilliferum (SP) is a rare tumor that develops from salivary glands. First described by Abrams and Finck, the tumor shows a papillary form. The most frequent location of this tumor is the palate. In this case, we report a 63 year old man with a lesion located at the right retromolar of the mandible which might have caused bone resorption. Among with this case report, this paper discuss the feature of this rare tumor. The cases were searched from the pubmed website (https://www.ncbi.nlm.nih.gov/pubmed/) using the keyword ‘sialadenoma papilliferum’. Out of total 65 cases 41 patients were male and 19 patients were female. 4 patients were under the age of 20, 4 patients were between 21 and 40, 25 patients were between 41 and 60, 23 patients were between 61 and 80 and 4 patients were over 81 years old. The number of patients with the size of the lesion less than 1cm were 23, 16 patients had the size of 1cm to 2cm and 8 patients lesion were larger than 2cm. 22 lesions were located at the hard palate, 2 were at the soft palate, 6 were at the junction of hard and soft palate, 8 were at the buccal mucosa, 1 was at the upper lip mucosa, 3 were at the retromolar pad, 2 were at the tongue, 4 were at the parotid gland, 2 were at the floor of mouth, 1 was at the faucial pillar, 3 were at the bronchus, 1 was at the esophagus, 2 were at the nasopharynx, 1 was at the nasal septum, 1 was at the lung, 1was at the nasal cavity. In this case the lesion was found at an unusual location and although SP is known to be a benign tumor it may present bone resorption and have malignant characteristics.

구강점막에 발생한 호지킨림프종의 증례보고 및 문헌고찰

이은수,HE YUZHU,조혜중,SUN QIAOCHU,SHI SHUHAN,김영,김옥준 대한구강악안면병리학회 2018 대한구강악안면병리학회지 Vol.42 No.6

Extranodal intraoral Hodgkin lymphoma is not common. We report the case of a 78-year-old male patient with ulcer of the mandibular oral mucosa that was not cured for about 3 weeks. In biopsy and histopathologic examination, it was found to be extranodal intraoral Hodgkin lymphoma. Early diagnosis of oral lesions led to early detection of lesions of the opposite neck lymph node in an additional PET-CT scan We report this case and review relevant literature

Development of Novel Photosensitizer Using the <i> Buddleja officinalis</i> Extract for Head and Neck Cancer

Cho, Hyejoung,Zheng, Hui,Sun, Qiaochu,Shi, Shuhan,He, YuZhu,Ahn, Kyuhyeon,Kim, Byunggook,Kim, Hye-Eun,Kim, Okjoon Hindawi 2018 Evidence-based Complementary and Alternative Medic Vol.2018 No.-

<P>Photodynamic therapy (PDT) is generally safer and less invasive than conventional strategies for head and neck cancer treatment. However, currently available photosensitizers have low selectivity for tumor cells, and the burden and side effects are so great that research is needed to develop safe photosensitizers. In this study, it was confirmed that the<I> Buddleja officinalis</I> (BO) extract, used in the treatment of inflammation and vascular diseases, shows fluorescence when activated by LED light, and, based on this, we aimed to develop a new photosensitive agent suitable for PDT. MTT, Diff-Quick® staining, and DCF-DA were performed to measure the effects of treating head and neck cancer cells with BO extract and 625 nm LED light (BO-PDT). Cell cycle, TUNEL, and western blot assays, as well as acridine orange staining, were performed to explore the mechanism of BO-PDT-induced cell death. We found that when the BO extract was irradiated with 625 nm LED light, it showed sufficient fluorescence and stronger intracellular toxicity and ROS effect than the currently commercially available hematoporphyrin. BO-PDT resulted in a decrease of mTOR activity that was correlated with an increase in the levels of ATG5, beclin-1, and LC3-II, which interfere with the formation of autophagosomes. In addition, BO-PDT induced the activation of PARP and led to an increase in the expression of proapoptotic protein Bax and a decrease in the expression of the antiapoptotic protein Bcl-2. Moreover, BO-PDT has been shown to induce the autophagy pathway 4 h after treatment, while apoptosis was induced 16 h after treatment. Finally, we confirmed that BO-PDT caused cell death of head and neck cancer cells via the intrinsic pathway. Therefore, we suggest that BO extract can be used as a new photosensitizer in PDT of head and neck cancer.</P>

OCT 광학기기의 레진 컴파짓 수복평가의 응용

Sang Mi Jeon,Young Hee Lee,Hyeong Joon Ji,Hye Joung Cho,Seo Jin Kim,Hye Eun Kim,SUN QIAOCHU,Kyu Hyeon Ahn,Hong Ran Choi,Ok Joon Kim 대한구강악안면병리학회 2016 대한구강악안면병리학회지 Vol.40 No.4

Composite resins are developed as restorative materials to improve esthetics and mechanical properties. To improve the physical properties of resin material, resin filler have to be added. However, no imaging method is adopted for resin filler distribution. Optical coherence tomography (OCT) is a optical imaging technique to delineate microscopic structures within biological tissue. The OCT application to dental composites resin and its filler is not described yet. So, this new and advanced optical method is needed for clinical application for evaluation of dental composite resin. To analyze the spatial distribution of dental composite resin and to evaluate the resin restoration in cavity, frequency domain optical coherence tomography (FD-OCT) was used for their analysis. Resin restored tooth was prepared. For morphological observation, serially sectioned teeth, conventional X-ray taking and micro computed-tomography (CT) images were compared with OCT images. The experiment has done to evaluate the success of the resin restoration using 3 dimensional structure OCT image. In this research, OCT is evaluated as a new technique to image resin restoration. The evaluation of resin restored tooth was performed by OCT. Inappropriate restoration such as marginal adaptation, large porosities, internal integrity and poor contour could be detected. Resin filler also could be checked by OCT. The distribution, number, regularity and size of resin filler can be differentiated from several commercial products. Considering the characteristics of the OCT, it can be used to evaluate the defects of resin restoration, resin filler distribution, and internal integrity between resin material and tooth structure. The OCT can be considered to be a new and advanced method for the evaluation of resin restorations.