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Moon, S.M.,Kim, J.S.,Kim, H.J.,Choi, M.S.,Park, B.R.,Kim, S.G.,Ahn, H.,Chun, H.S.,Shin, Y.K.,Kim, J.J.,Kim, D.K.,Lee, S.Y.,Seo, Y.W.,Kim, Y.H.,Kim, C.S. Society for Bioscience and Bioengineering, Japan ; 2014 Journal of bioscience and bioengineering Vol.117 No.5
A novel fibrinolytic enzyme was purified from Lyophyllum shimeji, a popular edible mushroom in Asia. The enzyme was purified using combination of anion exchange chromatography on a Mono Q 5/5 column and size exclusion gel filtration chromatography on Superdex 200 100/300 column. This purification protocol resulted 80.9-fold purification of the enzyme and a final yield of 5.7%. The molecular weight of the purified enzyme was estimated to be 21 kDa by SDS-PAGE and size exclusion gel filtration. The N-terminal amino acid sequence was found to be ITFQSASP, which is dissimilar from that of known fibrinolytic enzymes. The purified enzyme was a neutral protease with an optimal reaction pH and temperature of 8.0 and 37<SUP>o</SUP>C, respectively. Enzymatic activity was inhibited by Cu<SUP>2+</SUP> and Co<SUP>2+</SUP>. It was also significantly inhibited by PMSF and TPCK. Furthermore, it was found to exhibit a higher specificity for S-7388, a well-known chymotrypsin chromogenic substrate, indicating chymotrypsin like serine metalloprotease. The relative fibrinolytic activity of 5 μg purified enzyme have two fold more activity than 1 unit/ml of plasmin on fibrin plate. Furthermore, purified enzyme preferentially hydrolyzed the Aα-chain followed by the Bβ- and γ-chain of fibrinogen, which is precursor of fibrin. Therefore, these data suggests that the fibrinolytic enzyme derived from edible mushroom, L. shimeji, might be useful for thrombolytic therapy and preventing thrombotic disease.
( J. M. Kim ),( S. K. Lim ),( J. S. Moon ),( Nam H. M. ),( H. M. Kang ),( G. C. Jang ),( S. H. Wee ),( Y. S. Joo ),( S. C. Jung ) 한국미생물 · 생명공학회 2010 Journal of microbiology and biotechnology Vol.20 No.10
A total of 1,444 coagulase-negative staphylococci (CNS) isolates from bovine mastitic milk samples collected during 2003-2008 in Korea were identified to the species level. Of 14 species identified, S. simulans, S. haemolyticus, and S. sciuri accounted for over 60% of the isolates. All the CNS isolates were tested for susceptibility to eight antimicrobials commonly used in dairy cattle. With a few exceptions, similar resistance patterns were observed among the CNS species: penicillin and ampicillin showed the lowest activity, whereas amikacin, cephalothin, and gentamicin were highly effective. About 39% (557/1,444) of the CNS isolates were pan-susceptible, whereas 12% (175/1,444) showed resistance to four or more antimicrobials tested.
Wrinkle structures formed by formulating UV-crosslinkable liquid prepolymers
Park, S.K.,Kwark, Y.J.,Nam, S.,Park, S.,Park, B.,Yun, S.,Moon, J.,Lee, J.I.,Yu, B.,Kyung, K.U. IPC Science and Technology Press 2016 Polymer Vol.99 No.-
Artificial wrinkles have recently been in the spotlight due to their potential use in high-tech applications. A spontaneously wrinkled film can be fabricated from UV-crosslinkable liquid prepolymers. Here, we controlled the wrinkle formation by simply formulating two UV-crosslinkable liquid prepolymers, tetraethylene glycol bis(4-ethenyl-2,3,5,6-tetrafluorophenyl) ether (TEGDSt) and tetraethylene glycol diacrylate (TEGDA). The wrinkles were formed from the TEGDSt/TEGDA formulated prepolymer layers containing up to 30 wt% of TEGDA. The wrinkle formation depended upon the rate of photo-crosslinking reaction of the formulated prepolymers. The first order apparent rate constant, k<SUB>app</SUB>, was between ca. 5.7 x 10<SUP>-3</SUP> and 12.2 x 10<SUP>-3</SUP> s<SUP>-1</SUP> for the wrinkle formation. The wrinkle structures were modulated within the k<SUB>app</SUB> mainly due to variation in the extent of shrinkage of the formulated prepolymer layers with the content of TEGDA.
NUMERICAL CALCULATION OF TWO FLUID SOLAR WIND MODEL
KIM S.-J.,KIM K.-S.,MOON Y.-J.,CRO K.-S.,PARK Y. D. The Korean Astronomical Society 2004 Journal of The Korean Astronomical Society Vol.37 No.1
We have developed a two fluid solar wind model from the Sun to 1 AU. Its basic equations are mass, momentum and energy conservations. In these equations, we include a wave mechanism of heating the corona and accelerating the wind. The two fluid model takes into account the power spectrum of Alfvenic wave fluctuation. Model computations have been made to fit observational constraints such as electron($T_e$) and proton($T_p$) temperatures and solar wind speed(V) at 1 AU. As a result, we obtained physical quantities of solar wind as follows: $T_e$ is $7.4{\times}10^5$ K and density(n) is $1.7 {\times}10^7\;cm^{-3}$ in the corona. At 1 AU $T_e$ is $2.1 {\times} 10^5$ K and n is $0.3 cm^{-3}$, and V is $511 km\;s^{-1}$. Our model well explains the heating of protons in the corona and the acceleration of the solar wind.
Bae, J.S.,Park, S.H.,Jamiyandorj, U.,Kim, K.M.,Noh, S.J.,Kim, J.R.,Park, H.J.,Kwon, K.S.,Jung, S.H.,Park, H.S.,Park, B.H.,Lee, H.,Moon, W.S.,Sylvester, K.G.,Jang, K.Y. American Association of Pathologists and Bacteriol 2016 The American journal of pathology Vol.186 No.12
<P>Recently, the roles of sirtuins (SIRTs) in tumorigenesis have been of interest to oncologists, and protein kinase CK2 alpha 1 (CSNK2A1) has been shown to be involved in tumorigenesis by phosphorylating various proteins, including SIRT1. Therefore, we evaluated the roles of CSNK2A1, SIRT6, and phosphorylated SIRT6 and their relationships in breast carcinoma. Nuclear expression of CSNK2A1 and SIRT6 predicted shorter overall survival and relapse-free survival by multivariate analysis. Inhibition of CSNK2A1 decreased the proliferative and invasive activity of cancer cells. In addition, CSNK2A1 was bound to SIRT6 and phosphorylated SIRT6; evidence for this is provided from immunofluorescence staining, co-immunoprecipitation of CSNK2A1 and SIRT6, a glutathione S-transferase pull-down assay, an in vitro kinase assay, and transfection of mutant CSNK2A1. Knockdown of SIRT6 decreased the proliferation and invasiveness of cancer cells. Overexpression of SIRT6 increased proliferation, but mutation at the Ser338 phosphorylation site of SIRT6 inhibited the proliferation of MCF7 cells. Moreover, both knockdown of SIRT6 and a mutation at the phosphorylation site of SIRT6 decreased expression of matrix metallopeptidase 9, beta-catenin, cyclin D1, and NF-kappa B. Especially, SIRT6 expression was associated with the nuclear localization of B-catenin. This study demonstrates that CSNK2A1 and SIRT6 are indicators of poor prognosis for breast carcinomas and that CSNK2A1-mediated phosphorylation of SIRT6 might be involved in the progression of breast carcinoma.</P>
Kang, J.S.,Moon, Y.S.,Lee, S.H.,Park, I.K. Academic Press 2013 Pesticide biochemistry and physiology Vol.105 No.3
To determine the nematicidal mode of action of aliphatic compounds against the pinewood nematode (Bursaphelenchus xylophilus), we evaluated the inhibition activity of 63 aliphatic compounds on B. xylophilus acetylcholinesterases (BxACEs) and glutathione S-transferase. In the primary inhibition assay using B. xylophilus crude proteins, more than 65% of BxACE inhibition activity was observed for C<SUB>6</SUB>, C<SUB>9</SUB>, C<SUB>10</SUB>, and C<SUB>12</SUB> 2E-alkenals. Other compounds showed moderate or weak inhibition activity. The inhibition activity against 3 recombinant BxACEs was subsequently evaluated using active compounds in a primary inhibition assay. C<SUB>12</SUB> 2E-alkenal showed the strongest inhibition activity against BxACE-1, followed by C<SUB>9</SUB>, C<SUB>6</SUB>, and C<SUB>10</SUB> 2E-alkenals. The IC<SUB>50</SUB> values of C<SUB>12</SUB>, C<SUB>6</SUB>, C<SUB>10</SUB>, and C<SUB>9</SUB> 2E-alkenal against BxACE-2 were 0.0059, 0.57, 0.86, and 0.99mg/ml, respectively. C<SUB>12</SUB> 2E-alkenal showed the strongest inhibition activity against BxACE-3 followed by C<SUB>6</SUB> 2E-alkenal. In an inhibition activity test using glutathione S-transferase from the pinewood nematode, C<SUB>10</SUB>, C<SUB>9</SUB>, and C<SUB>6</SUB> 2E-alkenals and C<SUB>12</SUB> alkanoic acid showed >45% inhibition activity.
Role of Human Aquaporin 5 In Colorectal Carcinogenesis
Kang, S.K.,Chae, Y.K.,Woo, J.,Kim, M.S.,Park, J.C.,Lee, J.,Soria, J.C.,Jang, S.J.,Sidransky, D.,Moon, C. American Association of Pathologists and Bacteriol 2008 The American journal of pathology Vol.173 No.2
While overexpression of several aquaporins (AQPs) has been reported in different types of human cancer, the role of AQPs in carcinogenesis has not been clearly defined. Here, by immunochemistry, we have found expression of AQP5 protein in 62.8% (59/94) of resected colon cancer tissue samples as well as association of AQP5 with liver metastasis. We then demonstrated that overexpression of human AQP5 (hAQP5) induces cell proliferation in colon cancer cells. Overexpression of wild-type hAQP5 increased proliferation and phosphorylation of extracellular signal-regulated kinase-½ in HCT116 colon cancer cells whereas these phenomena in hAQP5 mutants (N185D and S156A) were diminished, indicating that both membrane association and serine/threonine phosphorylation of AQP5 are required for proper function. Interestingly, overexpression of AQP1 and AQP3 showed no differences in extracellular signal-regulated kinase-½ phosphorylation, suggesting that AQP5, unlike AQP1, may be involved in signal transduction. Moreover, hAQP5-overexpressing cells showed an increase in retinoblastoma protein phosphorylation through the formation of a nuclear complex with cyclin D1 and CDK4. Small interfering RNA analysis confirmed that hAQP5 activates the Ras signaling pathway. These data not only describe the induction of hAQP5 expression during colorectal carcinogenesis but also provide a molecular mechanism for colon cancer development through the interaction of hAQP5 with the Ras/extracellular signal-regulated kinase/retinoblastoma protein signaling pathway, identifying hAQP5 as a novel therapeutic target.
Kang, H.w.,Song, P.H.,Ha, Y.S.,Kim, W.T.,Kim, Y.J.,Yun, S.J.,Lee, S.C.,Choi, Y.H.,Moon, S.K.,Kim, W.J. Pergamon Press 2013 European journal of cancer Vol.49 No.14
Background: We investigated whether genetic polymorphisms in the glutathione S transferase mu (GSTM1) and theta (GSTT1) genes modulated risk, disease progression and survival in primary muscle invasive bladder cancer (MIBC). Methods: GSTM1 and GSTT1 polymorphisms were analysed by multiplex polymerase chain reaction (PCR) using blood genomic DNA in 110 MIBC patients and 220 gender- and age-matched healthy controls. The influence of the genetic polymorphisms on patient survival was evaluated by Kaplan-Meier survival curves and Cox Proportional Hazard models. We also evaluated whether cigarette smoking and treatment modality modified the association between genotype and prognosis. Results: GSTM1-null individuals exhibited increased risk for MIBC and an association with cigarette smoking. GSTT1-null subjects showed significant disease progression and cancer-specific death. In the combined analysis, GSTT1-null genotype was an independent risk factor for disease progression and cancer specific death regardless of GSTM1 genotype. Significant differences in progression-free survival (PFS) and cancer-specific survival (CSS) were seen based on GSTT1 genotype. The survival impact of the GSTT1 genotype was only valid for smokers. The GSTT1-null genotype was an independent prognostic factor for shorter PFS in patients who received chemotherapy and those who did not undergo radical cystectomy. By multivariate Cox regression analysis, GSTT1-null genotype was a predictive factor for disease progression and cancer specific survival regardless of treatment modality. Conclusions: The GSTM1-null genotype plays an important role in genetic susceptibility to MIBC and the GSTT1-null genotype is associated with disease progression and shorter survival in MIBC.
권용식(Y. S. Kwon),김종성(J. S. Kim),문형일(H. I. Moon),손미진(M. J. Sohn),천재승(J. S. Cheon) 한국자동차공학회 2013 한국자동차공학회 부문종합 학술대회 Vol.2013 No.5
The electric wedge brake (EWB) is an electro-mechanical brake actuator that shows promise for application in future brake by wire systems. Due to the system properties it has special system that supplies proper pedal feel reactions which means user actuating forces. The pedal feel generally considers critical issues which cause various complaints of car manufacturing company. However, making proper pedal feel is fussy task because of the system complexity. In this study, a Multi-objective robust design approach was applied to the design of the pedal simulator to adapt target pedal feel proposed mass-produced car as well as to improve the durability of system. The multiple regression methods are established to perform an approximated design optimization. Pareto optimal solutions for minimized gap of pedal feel and maximized S/N ratio characteristics present the robust optimal solution.