Global analysis of CpG methylation reveals epigenetic control of the radiosensitivity in lung cancer cell lines

Kim, E-H,Park, A-K,Dong, S M,Ahn, J-H,Park, W-Y Macmillan Publishers Limited 2010 Oncogene Vol.29 No.33

Epigenetic regulation by CpG methylation has an important role in tumorigenesis as well as in the response to cancer therapy. To analyze the mechanism of epigenetic control of radiosensitivity, the CpG methylation profiles of radiosensitive H460 and radioresistant H1299 human non-small cell lung cancer (NSCLC) cell lines were analyzed using microarray profiling. These analyses revealed 1091 differentially methylated genes (DMG) (absolute difference of mean β-values, |Δβ¯|>0.5), including genes involved in cell adhesion, cell communication, signal transduction and transcriptional regulation. Among the 747 genes hypermethylated in radioresistant H1299 cells, CpG methylation of SERPINB5 and S100A6 in radioresistant H1299 cells was confirmed by methylation-specific PCR. Reverse transcriptase–PCR showed higher expression of these two genes in radiosensitive H460 cells compared with radioresistant H1299 cells. Downregulation of SERPINB5 or S100A6 by small interfering RNA in H460 cells increased the resistance of these cells to ionizing radiation. In contrast, promoter CpG sites of 344 genes, including CAT and BNC1, were hypomethylated in radioresistant H1299 cells. Suppression of CAT or BNC1 mRNA expression in H1299 cells also reduced the resistance of these cells to ionizing radiation. Thus, we identified DMGs by genome-wide CpG methylation profiling in two NSCLC cell lines with different responses to ionizing radiation, and our data indicated that these differences may be critical for epigenetic regulation of radiosensitivity in lung cancer cells.

The prolyl isomerase Pin1 interacts with a ribosomal protein S6 kinase to enhance insulin-induced AP-1 activity and cellular transformation

Lee, N. Y.,Choi, H.-K.,Shim, J.-H.,Kang, K.-W.,Dong, Z.,Choi, H. S. Oxford University Press 2009 Carcinogenesis Vol.30 No.4

<P>Phosphorylation of proteins on serine or threonine residues that immediately precede proline (pSer/Thr-Pro) is specifically catalyzed by the peptidyl-prolyl cis-trans isomerase Pin1 and is a central signaling mechanism in cell proliferation and transformation. Although Pin1 is frequently overexpressed in hepatocellular carcinoma (HCC), the molecular mechanism of Pin1 in HCC has not been completely elucidated. Here, we show that Pin1 interacts with p70S6K in vitro and ex vivo. Overexpression of Pin1 resulted in enhanced p70S6K phosphorylation induced by insulin in SK-HEP-1 cells. In contrast, Pin1(-/-) mouse embryonic fibroblasts (MEFs) exhibited significantly decreased insulin-induced p70S6K phosphorylation compared with Pin1(+/+) MEFs. Furthermore, Pin1 enhanced the insulin-induced extracellular signal-regulated protein kinase (ERK)1/2 phosphorylation through its interaction with p70S6K, whereas the inhibition of p70S6K activity by rapamycin suppressed insulin-induced ERK1/2 phosphorylation in SK-HEP-1 cells. Hence, Pin1 affected activator protein-1 activity through p70S6K-ERK1/2 signaling in SK-HEP-1 cells. Most importantly, Pin1-overexpressing JB6 Cl41 cells enhanced neoplastic cell transformation promoted by insulin much more than green fluorescent protein-overexpressing JB6 Cl41 control cells. These results imply that Pin1 amplifies insulin signaling in hepatocarcinoma cells through its interaction with p70S6K, suggesting that Pin1 plays an important role in insulin-induced tumorigenesis and is a potential therapeutic target in hepatocarcinoma.</P>

China Spallation Neutron Source: Accelerator Design Iterations and R&D Status

J. Wei,C.-D. Deng,C.-H. Wang,C.-T. Shi,H. Sun,H.-F. Ouyang,H.-M. Qu,H.-Y. Dong,J. Li,J. Zhang,J.-S. Cao,J.-Y. Tang,L. Dong,L.-L. Wang,Q. Qin,Q.-B. Wang,S. Wang,S.-N. Fu,S.-X Fang,T. -G. Xu,W. Kang,Y.- 한국물리학회 2007 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.50 No.I

The China Spallation Neutron Source (CSNS) is a high-power, accelerator-based project currently under preparation. The accelerator complex consists of an H$^-$ ion source, an H$^-$ linac, a rapid-cycling proton synchrotron, and the transport lines. During the past year, the design of most accelerator systems went through major iterations, and initial research and developments was started on the prototyping of several key components.

A structural strain method for low-cycle fatigue evaluation of welded components

Dong, P.,Pei, X.,Xing, S.,Kim, M.H. Applied Science Publishers ; Elsevier Science Ltd 2014 The International journal of pressure vessels and Vol.119 No.-

In this paper, a new structural strain method is presented to extend the early structural stress based master S-N curve method to low cycle fatigue regime in which plastic deformation can be significant while an elastic core is still present. The method is formulated by taking advantage of elastically calculated mesh-insensitive structural stresses based on nodal forces available from finite element solutions. The structural strain definition is consistent with classical plate and shell theory in which a linear through-thickness deformation field is assumed a priori in both elastic or elastic-plastic regimes. With considerations of both yield and equilibrium conditions, the resulting structural strains are analytically solved if assuming elastic and perfectly plastic material behavior. The formulation can be readily extended to strain-hardening materials for which structural strains can be numerically calculated with ease. The method is shown effective in correlating low-cycle fatigue test data of various sources documented in the literature into a single narrow scatter band which is remarkable consistent with the scatter band of the existing master S-N curve adopted ASME B&PV Code since 2007. With this new method, some of the inconsistencies of the pseudo-elastic structural stress procedure in 2007 ASME Div 2 Code can now be eliminated, such as its use of Neuber's rule in approximating structural strain beyond yield. More importantly, both low cycle and high cycle fatigue behaviors can now be treated in a unified manner. The earlier mesh-insensitive structural stress based master S-N curve method can now be viewed as an application of the structural strain method in high cycle regime, in which structural strains are linearly related to traction-based structural stresses according to Hooke's law. In low-cycle regime, the structural strain method characterizes fatigue damage directly in terms of structural strains that satisfy linear through-thickness deformation gradient assumption, material nonlinear behavior, and equilibrium conditions. The use of a pseudo-elastic structural stress definition is not fundamental, but merely a means to put low-cycle and high-cycle fatigue test data in a conventional stress-based S-N data representation which is typically preferred in engineering practice, than a strain-based representation.

유리系 內의 Ferrite의 Mossbauer Spectra

安振盛,金俊植,李敬行,趙壽烈,董徹,洪致裕 동국대학교 자연과학연구소 1984 자연과학연구 논문집 Vol.4 No.-

本 硏究는 5NiO-5CdO-10Fe₂O₃-30Na₂CO₃-10SiO₂(mole %)유리계에 대한 Mossbauer parameter의 溫度依存性을 조사하였다. Mossbauer spectrum은 한쌍의 Quadrupole doublet의 중첩으로 fitting하였으며 이중 하나는 tetrahedral site,다른 하나는 octahedral site이다. 상대적인 共鳴吸收面積比로부터 octahedral site와 tetrahedral site에 대한 Debye temperature를 계산하여 본 결과 각각 418K, 449K 임을 알았다. 또한 θ?값들을 이용해 least square method로 isomer shiftdral site의 壓力依存系數 α와 2.97×10?mm/sec K, I.S(0)일때의 를 평가해본 결과 octahedral site의 α는 2.97×10?mm/sec K,I.S(0)는 tetrahedral site이고 α의 3.2305×10?mm/sec K, I.S(0)는 0.276mm/sec임을 알았다. 그리고 quadrupole splitting은 측정온도가 증가함에 따라 서서히 감소함을 알았다. The temperature dependence of the Mossbauer parameter for 5NiO-5CdO-10Fe₂O₃-30Na₂CO₃-10SiO₂(mole %) quenched glass system was investigated. The spectra were fitted as superposition of a pair of quadrupole double ; One is for the tetrahedral site and the other is for the octahedral site. From the relative absorption area ratios of the spectra, the Debye erature of two sites were calculated to be 418K and 449K for the octahedral site and the ?edrai site, respectively. From these Debye temperatures, We can see that the isomer shifts and the coefficients αof the pressure dependence of the isomer shift are 0.456mm/sec, 2.97×10?mm/sec K for octahedral site and 0.276mm/sec K, 3.2305×10?mm/sec K for ?edral site. Also, we can see that the quadrupole splittings of two sites are gradually decresed the increase of the measuring temperature.

Production of Hydrogen-Rich Syngas from Dimethyl Ether by Using a Microwave Steam Plasma

Han S. Uhm,Chang H. Choi,Joo Y. Lee,Dong J. Kim,Sang G. Woo,Minwoo Woo,Dong C. Lee 한국물리학회 2019 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.75 No.6

Dimethyl ether (DME) is disintegrated by a microwave steam-plasma raising the gas temperature in a reaction chamber of 100 liters in a swirl-type gasifier. With additional heating of DME by partial oxidation, the gasifier temperature reaches 1250 °C. The initial breakdown species of DME molecules are hydrogen, carbon monoxide and methane, which eventually disintegrate to CO and H2 by steam modification at temperatures higher than 1200 °C. The relative concentrations of hydrogen, carbon monoxide and carbon dioxide produced solely from DME modification by steam were 67.5 percent, 30.3 percent and 2.2 percent, respectively, achieving a conversion rate of 88 percent at a temperature of 1058 °C. The total energy efficiency of the present experiment was 81 percent.

Practices and predictors of 2009 H1N1 vaccination in cancer patients: a nationwide survey in Korea.

Shin, Dong W,Kim, Yeol,Park, Jong H,Cho, Juhee,Jho, Hyun J,Yang, Hyung-Kook,Kim, Hyun S,Kim, So Y Blackwell Publishing Ltd 2012 Influenza and other respiratory viruses Vol.6 No.6

<P>Because patients with cancer are considered to be at high-risk for influenza infection and related complications, annual vaccination is recommended. The emergence of the novel H1N1 influenza virus in 2009 complicated the medical care of patients with cancer. The present study examined H1N1 vaccination practices among patients with cancer during the pandemic season and investigated factors related to the vaccination.</P>

간호전공과 비 간호전공 여대생의 모유수유 지식과 태도, 실천 지속 의지에 대한 연구

김수정,김희진,동민영,박소민,송예슬,이고은,이승만,장지은,정유진,최예슬 이화여자대학교 간호과학대학 2013 이화간호학회지 Vol.- No.47

Purpose: This study was conducted as benchmark to improve breast-feeding educational programs by comparing knowledge, attitude, and practical volition between female nursing college students and non-nursing college students. Method: The survey was conducted on 236 female students from ‘E’ and ‘Y’ Universities which were located in Seoul. The sample consisted of 114 nursing and 122 non-nursing college students. The data were collected using self-administered questionnaires from October 25 to December 17, 2012. The collected data were analyze dusing SPSS20.0. Result: The nursing college students’ level of knowledge, attitude and practical volition toward breast feeding was higher than non-nursing college students. There was positive correlation between knowledge and practical volition and attitude and practical volition about breast-feeding in nursing college students. But non-nursing college students had positive correlation between knowledge and attitude, and knowledge and practical volition about breast-feeding. Conclusion: Based on the results of the research, there was a correlation between knowledge, attitude, and practical volition toward breast feeding. It was suggested that technical knowledge of breast feeding be offered to female college students, as well as nursing college students. It could help them have the right information on breast feeding. It was also suggested that educational programs to reinforce emotional⋅practical attitude based on the knowledge of breast feeding be developed. Also, this study suggested that female students get their practical education in breast feeding under any circumstances with the practical volition to breast feed. Improving the knowledge, attitude, and the practical volition toward breast feeding, will increase the rate of breast feeding.

Deletions of 9p21 and TP53 in Bladder Cancer

Park, W.-S Korean Academy of Medical Science 1996 Journal of Korean medical science Vol.11 No.3

게임 도메인 개체명인식과 딥러닝 의도분류를 통한 주제대화 성능향상 기술

윤재민(Jae-Min Yun),지민성(Min-Seong Jee),신동춘(Dong-Chun Shin),고연정(Yeon-Jeong Ko) 한국컴퓨터정보학회 2021 한국컴퓨터정보학회 학술발표논문집 Vol.29 No.1