Diagnostic usefulness of a T cell-based assay for latent tuberculosis infection in kidney transplant candidates before transplantation

Kim, S.-H.,Lee, S.-O.,Park, I.-A.,Park, S.J.,Choi, S.-H.,Kim, Y.S.,Woo, J.H.,Park, S.-K.,Park, J.S.,Kim, S.C.,Han, D.J. Blackwell Publishing Inc 2010 Transplant infectious disease Vol.12 No.2

<P>S.-H. Kim, S.-O. Lee, I.-A. Park, S.J. Park, S.-H. Choi, Y.S. Kim, J.H. Woo, S.-K. Park, J.S. Park, S.C. Kim, D.J. Han. Diagnostic usefulness of a T cell-based assay for latent tuberculosis infection in kidney transplant candidates before transplantation.Transpl Infect Dis 2010: <B>12:</B> 113–119. All rights reserved</P><P>Background</P><P>The presence of latent tuberculosis (TB) infection (LTBI) should be evaluated before kidney transplantation. Although a new T cell-based assay for diagnosing LTBI gave promising results, this assay has not yet been compared with the tuberculin skin test (TST) for diagnosing LTBI in renal transplant candidates before transplantation.</P><P>Patients and methods</P><P>All adult patients admitted to a single institute for renal transplantation over a 1-year period were prospectively enrolled. A clinically predictive risk of LTBI was defined as: (i) recent close contact with a person with pulmonary TB; (ii) abnormal chest radiography; (iii) a history of untreated or inadequately treated TB; or (iv) a new infection (i.e., a recent conversion of TST).</P><P>Results</P><P>Of 209 renal recipients, 47 (22%) had a positive TST≥5 mm, 21 (10%) had a positive TST≥10 mm, 65 (30%) had a positive T-SPOT.<I>TB</I> test, and 25 (12%) had an indeterminate T-SPOT.<I>TB</I> test. The induration size of TST was significantly associated with a high positivity rate on T-SPOT.<I>TB</I> (<I>P</I><0.001). Agreement between T-SPOT.<I>TB</I> test and TST≥10 mm was fair (<I>k</I>=0.24, 95% confidence interval 0.11–0.36). However, neither univariate nor multivariate analysis showed any association between the clinical risk for LTBI and positivity on T-SPOT.<I>TB</I> or TST.</P><P>Conclusion</P><P>T-SPOT.<I>TB</I> test was more frequently positive than TST in renal transplant candidates. However, further longitudinal studies are awaited to determine whether the ability of T-SPOT.<I>TB</I> assay to detect LTBI in renal transplant recipients can better predict the development of TB than can TST after transplantation.</P>

Clinical and histopathological study of Charcot-Marie-Tooth neuropathy with a novel S90W mutation in BSCL2.

Choi, B-O,Park, M-H,Chung, K W,Woo, H-M,Koo, H,Chung, H-K,Choi, K-G,Park, K D,Lee, H J,Hyun, Y S,Koo, S K Oxford University Press 2013 Neurogenetics Vol.14 No.1

<P>The objective of the study was to investigate the disease-causing mutation in an autosomal dominant Charcot-Marie-Tooth disease type 2 family and examine the clinical and histopathological evaluation. We enrolled a family of Korean origin with axonal Charcot-Marie-Tooth disease neuropathy (FC305; 13 males, six females) and applied genome-wide linkage analysis. Whole exome sequencing was performed for two patients. In addition, sural nerve biopsies were obtained from two patients. Through whole exome sequencing, we identified an average of 20,336 coding variants from two patients. We also found evidence of linkage mapped to chromosome 11p11-11q13.3 (LOD score of 3.6). Among these variants in the linkage region, we detected a novel p.S90W mutation in the Berardinelli-Seip congenital lipodystrophy 2 (BSCL2) gene, after filtering 31 Korean control exomes. Our p.S90W patients had frequent sensory disturbances, pyramidal tract signs, and predominant right thenar muscle atrophy in comparison with reported p.S90L patients. The phenotypic spectra were wide and demonstrated intrafamilial variability. Two patients with different clinical features underwent sural nerve biopsies; the myelinated fiber densities were increased slightly in both patients, which differed from two previous case reports of BSCL2 mutations (p.S90L and p.N88S). This report expands the variability of the clinical spectrum associated with the BSCL2 gene and describes the first family with the p.S90W mutation.</P>

Melatonin enhances arsenic trioxide-induced cell death via sustained upregulation of Redd1 expression in breast cancer cells

Yun, S.M.,Woo, S.H.,Oh, S.T.,Hong, S.E.,Choe, T.B.,Ye, S.K.,Kim, E.K.,Seong, M.K.,Kim, H.A.,Noh, W.C.,Lee, J.K.,Jin, H.O.,Lee, Y.H.,Park, I.C. North-Holland 2016 Molecular and cellular endocrinology Vol.422 No.-

Melatonin is implicated in various physiological functions, including anticancer activity. However, the mechanism(s) of its anticancer activity is not well understood. In the present study, we investigated the combined effects of melatonin and arsenic trioxide (ATO) on cell death in human breast cancer cells. Melatonin enhanced the ATO-induced apoptotic cell death via changes in the protein levels of Survivin, Bcl-2, and Bax, thus affecting cytochrome c release from the mitochondria to the cytosol. Interestingly, we found that the cell death induced by co-treatment with melatonin and ATO was mediated by sustained upregulation of Redd1, which was associated with increased production of reactive oxygen species (ROS). Combined treatment with melatonin and ATO induced the phosphorylation of JNK and p38 MAP kinase downstream from Redd1 expression. Rapamycin and S6K1 siRNA enhanced, while activation of mTORC1 by transfection with TSC2 siRNA suppressed the cell death induced by melatonin and ATO treatment. Taken together, our findings suggest that melatonin enhances ATO-induced apoptotic cell death via sustained upregulation of Redd1 expression and inhibition of mTORC1 upstream of the activation of the p38/JNK pathways in human breast cancer cells.

14.6 A GeV ^28Si 중이온이 원자핵건판내에서 발생시킨 핵반응에서 생성된 2차입자의 발생각 분포

김종오,김태연,남신우,신택수,우종관,이세병,임계엽,장세덕,조재희,천병구,임인택,김기영 慶尙大學校 기초과학연구소 1990 基礎科學硏究所報 Vol.6 No.-

14.6A GeV^28Si 중이온이 원자핵 건판내에서 발생시킨 N_h=1인 핵반응에서 생성된 47개의 파쇄 α 입자와 537개의 단일하전 2차입자의 발생각들을 측정하여 변수 exp(γ-η_b)의 포괄적 분포를 회귀함수 dN=exp[a+χ{exp(γ-η_b)d{exp(γ-η_b)}로 적합시켰다. 여기서 의사신속도 γ=arctanh(cosθ)=-ln tan(θ/2)이고, 입사 중이온의 신속도 η_b=3.445이다. 그 적합결과 파쇄 α입자의 경우 χ=-0.052±0.011이고, 파쇄 p입자의 경우 χ=-0.141±0.015이었다. For LS emission angles of 47 α fragments and 537 single-charged shower particles, produced by the N_h (the number of heavyprongs)=1 interactions of 14.6 A GeV^28Si nuclei in the nuclear emulsion, the distribution of the parameter exp(γ-η_b) is well expressed by dN=exp[a+χ{exp(γ-η_b)d{exp(γ-η_b)}with χ=-0.052±0.011 for αfragments and χ=-0.141±0.015 for p 'fragments', where the pseudorapidity of secondaries γ=arctanh(cosθ)=-ln tan(θ/2) and the rapidity of incident heavy ions, η_b=3.445.

Synthesis of Iron Oxide Nanowires by Heating Iron Foils

Kim, Hyoun Woo,Shim, S.H.,O, B.H.,Lee, S.G.,Park, S.G.,Lee, El Hang Trans Tech Publications, Ltd. 2007 Key Engineering Materials Vol.342-343 No.-

<P>We have fabricated the iron oxide nanowires directly from iron foils through the simple heating in N2 ambient. We have characterized the samples by means of scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray (EDX) spectroscopy, and selected area diffraction pattern. The EDX spectrum revealed that the nanowires contained elements of Fe and O. The iron oxide nanowires were crystalline with diameters in the range of 30-200 nm. We have discussed the possible growth mechanisms.</P>

Phase III Clinical Trial for Immunogenecity and Safety of a New Recombinant Hepatitis B Virus Vaccine (HG-II) in the Newborn

Cho, Y. K 大韓臨床藥理學會 1996 臨床藥理學會誌 Vol.4 No.2

水溶性色素分析에 關한 考察

우세홍,윤오섭 최신의학사 1978 最新醫學 Vol.21 No.2

Thin-layer chromatography with Silicagel and cellulose powder was applied to water-soluble dyes. Silicagel and cellulose powder chromatoplates used were prepared under different conditions of activation. Using six developing solvent, the influence of the condition for activation upon the separation of water-soluble dyes was investigated. The results are shown Table 3. Methyl ethyl ketone+water (20:1.0) mixture and methyl ethyl ketone+acetone+water (10:0.1:0.4) mixture gave clear separation for water-soluble dyes. Including fluorescein, erythrosine, eosine, Rose bengale, phloxine, naphthol yellow S and acid red those Rf. values decrease in the described ordor. Methyl ethyl ketone+water (20:1.0) was applied to two adsurbents which were purchased from different manufactures. The results of chromatograms are obtained Figure 3.

식용유의 저장조건에 따른 산패도 비교

우세홍,김선덕,윤오섭 최신의학사 1978 最新醫學 Vol.21 No.2

Soybean oil were studied under four experimental storage conditions. Samples were exposed to sunlight irradiation at 30-35℃, irradiated with fluorescent light at 20-25°C, placed in the dark at 23-27°C and stored in a refrigerator at 5℃ for 1 month. Rancidity development was estimated by determining the peroxide values and the free acid values of the samples at appopriate intervals and obtained results were as follow. 1. The direct sunlight showed by far the strongest oxidant activity whereas the darkness showed the weakest. 2. The high temperature showed by far the strongest oxidant activity whereas the low temperature showed the weakest.

자궁의 임신의 MTX 국소 및 전신적인 보존치료의 임상비교

임옥경,문혜성,김종일,신정현,장병우,우복희 대한산부인과내시경학회 1994 대한산부인과내시경학회잡지 Vol.6 No.1

漢江의 越冬鳥類

元炳旿,禹漢貞,金相旭,具太會,李斗杓,崔東信 경희대학교 한국조류연구소 1986 연구보고 Vol.1 No.-

The Han River is an important river where some twentys pecies of waterbirds yearly visit for staging or wintering-including some species of surface-water (or inland-water ducks), geese, and diving ducks which are dominant. The river, which has the capacity for more than 15,000 waterbirds and which is located in their migrating route, is considered essential for their surviving. As we know, the accepted ornithological criteria for assigning international importance to wetlands as propounded by the International Wetlands Conferences in Ramar, Iran(1972) and Heiligenhafen, West Germany(1974) are: (ⅰ) the wetland should support at least 1% of the population of one or more species of waders or waterfowl in an international flyway. In the present context this constitutes Europe and North Africa; (ⅱ) the wetland should support totals of 20,000 or more waders and/or more than 10,000 wildfowl of all species. However, the number of the waterfowl visiting the Han River every year exceeds the figures suggested above for wetland criteria in Europe or North Africa. Thus, the Han River is regarded very important both domestically and internationally. The decrease in the past twenty years in the number of waterfowl and waders migrating to this river is largely due to the water pollution caused by industrial sewage and insecticides flowing into Han River and to the development of agricultural and recreation areas within the river which have encroached and impoverished habitats. For minimizing negative ecological impacts on wildfowl from the factors mentioned above and from Han River developing projects, constant efforts should be made for effective supervision and rational administration for nature conservation through scientific surveys and researches.