http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Expression of a Bovine β-Casein/Human Lysozyme Fusion Gene in the Mammary Gland of Transgenic Mice
Ryoo, Zae-Young,Lee, Won-Kyu 가톨릭 의과학연구원 1997 가톨릭 의과학연구원 국제학술대회 Vol.1 No.-
Transgenic mice containing a bovnie β-casein/human lysozyme fusion gene (pBZ) were generated in order to produce human lysozyme in mice milk. The expression vector was a quadripartite fusion consisting of a 2 kb upstream DNA of the bovine β-casein gene, human lysozyme gene, intron II of rabbit β-globin gene, and the polyadenylation/termination signals of SV4O DNA. Fertilized mouse zygotes were injected with pBZ, then transferred into the oviduct of foster mothers. Out of 20 mice born, 11 survived until post-weaning and three were identified as positive transgenic by Southern blot analysis (one male and two females), The founder mice were mated to BCF1 mice to produce the transgenic progeny. It was confirmed by RT-PCR and Northern blot analyses that the transgene was specifically expressed in the mammary gland of founder mice. Furthermore, the artificial introns within the transgenic RNA was proven to be correctly spliced out as judged by RT-PCR analysis. These results indicated that the mammary gland of transgenic mice generated in this study properly expressed the human lysozyme RNA in their mammary gland.
Embryonic Stem Cell-Preconditioned Microenvironment Effects on Epidermoid Carcinoma
Ryoo, Zae Young,Kim, Myoung Ok The Korean Society of Animal Reproduction 2012 Reproductive & developmental biology Vol.36 No.4
Embryonic stem cell-preconditioned microenvironment is important for cancer cells properitities by change cell morphology and proliferation. This microenvironment induces cancer cell reprogramming and results in a change in cancer cell properties such as differentiation and migration. The cancer microenvironment affects cancer cell proliferation and growth. However, the mechanism has not been clarified yet. Using the ES-preconditioned 3-D microenvironment model, we provide evidence showing that the ES microenvironment inhibits proliferation and reduces oncogenic gene expression. But ES microenvironment has no effect on telomerase activity, cell viability, cellular senescence, and methylation on Oct4 promoter region. Furthermore, methylation of Nanog was increase on ES-preconditioned microenvironment and supports results that no difference on RNA expression levels. Taken together, these results demonstrated that in the ES-preconditioned 3-D microenvironment is a crucial role for cancer cell proliferation not senescence.
Vasopressin-SV40T Antigen Expression in Transgenic Mice Induces Brain Tumor and Lymphoma
Ryoo, Zae-Young 가톨릭중앙의료원 가톨릭암센터 2002 암심포지움 Vol.- No.2
Little is known about the regulatory mechanisms of vasopressin(VP) gene expression. To understand the importance of various cis-acting elements in the regulation of VP gene expression, we have produced the transgenic mice regulated by VP constructs containing 3.8 kb of the 5'flanking region and all the exons and introns in the mouse VP gene, which was fused at the end of exon 3 to a SV40 T antigen(Tag). The two VP-transgene constructs differed by the lengths of their VP gene 3'flanking regions(2.1 versus 3.6 kb). In pVPSV.IGR3.6 construct, all six of founder transgenic mice were died at the age of 2∼6 weeks. Among three transgenic mice in pVPSV.IGR2.1 construct, one transgenic line expressing high levels of SV40 Tag was propagated. The founder and all transgene positive adult mice have appeared with shorten mortality or apparent phenotypic abnormalities and 21% mice showed brain tumor at 5 week and 100% mice showed brain tumor after 24 week. Histological analysis of transgenic mice showed that tumor developed in brain is similar to primitive neuroectodermal tumors (PNET) and tumor in spleen and lymph node is similar to lymphoma in human. The phenotype differences the two transgenic mice, therefore, suggest that the tissue-specific expression might be regulated by cis-acting elements in 1.5 kb 3' flanking region. Which is not contained in pVPSV.IGR2.1. Therefore, the pVPSV.IGR2.1 mice will provide a valuable mouse model system for studying PNETs tumorigenesis in brain and for studying tumorigenesis in lymph node and spleen.
GH Increases the Progesterone at Peri-estrus Stage in Mice Co-injected with PMSG for Superovulation
Kim, Young-Gee,Ryoo, Zae-Young,Park, Young-Sik The Korean Society of Animal Reproduction 2011 Reproductive & developmental biology Vol.35 No.4
Growth hormone (GH) is obligatory for growth and development. But, there is controversy on the GH effect about reproductive processes of sexual differentiation, pubertal maturation, gonadal steroidogenesis, gametogenesis and ovulation. This study was conducted to investigate the effect of GH on estrus, ovulation and embryo implantation. The results obtained were as follows. GH stimulated to increase estrus rate (p<0.05), pregnancy rate (p<0.05), and total fetus number in mice treated for superovulation. Also, the correlation between GH and steroids, E2 and P4, at peri-estrus stage/ peri-ovulation stage/ peri-implantation stage of the superovulation-induced mice was examined. Consequently, GH co-injected with PMSG especially increased P4 level (p<0.05) at peri-estrus stage of superovulationinduced mice. In conclusion, GH co-treatment in superovulation system boosted the rate of estrus, pregnancy and total fetus by increasing progesterone level at peri-estrus stage of superovulation-induced mice.