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RISS 인기검색어
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- 저자 : Roger A. Korus (검색결과 2 건)
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SUH, HYUNG-WON,CRAWFORD, DONG L.,KORUS, ROGER A. 한국미생물 · 생명공학회 1991 Journal of microbiology and biotechnology Vol.1 No.2
The culture conditions and nutritional requirements for enhanced mycelial growth of the ectomycorrhizal fungus P. tinctorius SMF were determined in flask scale experiments. Optimum culture conditions for growth of P. tinctorius SMF in a further modified Melin-Norkrans broth were as follows; temperature 25∼27℃, agitation 120rpm, and pH 4.0. P. tinctorius SMF utilized various carbon sources including monosaccharides, disaccharides, and polysaccharides. D-Glucose and mannitol were respectively the first and second most suitable carbon sources for mycelial growth. With D-Glucose as the principal carbon source, supplementation of modified Melin-Norkrans (MMN) broth with Lysine (800㎎/ℓ), Glutamic Acid (500㎎/ℓ), or Proline (50㎎/ℓ) enhanced mycelial yields 63%, 34%, and 22% respectively as compared to growth in medium lacking amino acids. Thiamin-HCl+biotin+pyridoxine supplementation also enhanced growth. As compared to mycelial growth in the MMN medium, growth of P. tinctorius SMF was enhanced 120% in MMN broth when the carbon/nitrogen ratio was 25/1 in citrate buffer at pH 4.5, and growth was 50% greater in MMN broth of carbon/nitrogen ratio with a 10/1∼20/1 without using the buffer. Standard conditions established for growth of P. tinctorius SMF in MMN broth were 25∼27℃, agitation 120rpm, buffered to pH 4.0 with citrate, in MMN medium containing 10g/ℓ D-glucose supplemented with 800㎎/ℓ lysine. In this medium the carbon/nitrogen ratio was 20/1∼25/1, and the maximal mycelial yield (Y_m/s) was 0.472 (4.72㎎/㎖) after 7 days of incubation, as compared to 0.214 (2.14㎎/㎖), when the fungus was grown in standard MMN broth.
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Yeast Single-Cell Protein Production Using Potato Processing Waste Water
Park, Eung Yeal,Don L. Crawford,Roger A. Korus,Richard C. Heimsch 한국미생물 · 생명공학회 1991 Journal of microbiology and biotechnology Vol.1 No.3
Four species of yeast, Saccharomyces cerevisiae, Candida utilis, Saccharomycopsis fibuligera, and Schwanniomyces castellii were evaluated for their ability to bloconvert potato processing waste water into microbial protein and the resulting single-cell proteins were evaluated as protein sources for rainbow trout, using in vitro analyses. The studies indicated that Schwanniomyces castellii, which utilizes starch dircetly and converts it into cell mass efficiently, was suitable for the bioconversion. In the single-stage continuous bioconversion, the yield S. casteliii cell mass, which contained approximately 37% protein, was 77%, at dilution rate 0.25 h^-1. Reduction of total carbohydrate was 81%. During batch fermentations, cell mass yield was about 72% and total carbohydrate reduction was 81%. Among the yeasts tested, S. castellii possessed the most fragile cell wall and had a favorable amino acid profile for salmonid fish; protein score of 86% (Met). In an in vitro pepsin digestibility test 80% digestibility (23∼38% above control) was observed when cells were pre-heated in a steam bath for 30 min. Results presented should be regarded as being preliminary in nature because they were derived from single experiments.
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