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Ro-Ui Kim(김로의),Soon-Cheol Ahn(안순철),Sun-Nyoung Yu(유선녕),Kwang-Youn Kim(김광연),Jong-Hwan Seong(성종환),Young-Guen Lee(이영근),Han-Soo Kim(김한수),Dong-Seob Kim(김동섭) 한국생명과학회 2011 생명과학회지 Vol.21 No.2
본 연구의 목적은 두유 curd를 형성하는 미생물을 분리하는 것이다. 두유 curd를 형성하는 미생물은 채소를 젖산균으로 발효시킨 전통적인 한국의 음식, 김치로부터 분리하였다. 분리 균주 196개 중 10개의 균주(strain No. 2-2-2, 2-15-2, 2-18-1, 2-19-2, 3-4-1, 3-4-2, 3-8-1, 3-8-3, 3-17-1, 4-39-5)가 단단한 두유 curd를 형성하였고 분자생물학적?생화학적 분석법에 의해 동정되었다. 분리균주로부터 추출한 genomic DNA는 16S rDNA 지역의 PCR 증폭을 위한 주형으로 사용하였다. GenBank 데이터로 16S rDNA 염기서열을 비교한 결과, 분리 균주들은 Leuconostoc mesenteroides group과 Lactobacillus sakei group으로 동정되었다. 두유 curd를 형성하는 균주들의 계통 발생학적 위치와 분류군은 neighbor-joining 방법을 이용하여 확인하였다. 또한, L. mesenteroides group은 생화학적 특성에 의해 L. mesenteroides subsp. dextranicum으로 동정되었다. 하지만 L. sakei group은 생화학적 특성 비교시 다양성을 보여 Lactobacillus sp.로 명명하였다. The purpose of this study was to isolate soy curd forming bacterial strains. Soy curd forming bacteria were isolated from Kimchi, a traditional Korean vegetable food that is fermented using lactic acid bacteria. Among 196 bacterial strains, ten isolates (strain No. 2-2-2, 2-15-2, 2-18-1, 2-19-2, 3-4-1, 3-4-2, 3-8-1, 3-8-3, 3-17-1, 4-39-5) formed firm soy curd. The isolated bacterial strains were identified by molecular biological and biochemical analyses. The genomic DNAs extracted from the isolated bacterial strains were used as a template for PCR amplification of 16S rDNA region. By comparing the results of the 16s rDNA sequences with GenBank data, the isolated strains were identified as Leuconostoc mesenteroides group and Lactobacillus sakei group. The phylogenetic position of soy curd forming strains and their related taxa were investigated using neighbor-joining method. L. mesenteroides group was further identified as L. mesenteroides subsp. dextranicum based on biochemical properties. L. sakei group was named Lactobacillus sp., because it showed a variety of biochemical properties.
Lee, Su Ui,Kim, Mun-Ock,Kang, Myung-Ji,Oh, Eun Sol,Ro, Hyunju,Lee, Ro Woon,Song, Yu Na,Jung, Sunin,Lee, Jae-Won,Lee, Soo Yun,Bae, Taeyeol,Hong, Sung-Tae,Kim, Tae-Don Korean Society for Molecular and Cellular Biology 2021 Molecules and cells Vol.44 No.1
Airway mucus secretion is an essential innate immune response for host protection. However, overproduction and hypersecretion of mucus, mainly composed of the gel-forming MUC5AC protein, are significant risk factors for patients with asthma and chronic obstructive pulmonary disease (COPD). The transforming growth factor β (TGFβ) signaling pathway negatively regulates MUC5AC expression; however, the underlying molecular mechanism is not fully understood. Here, we showed that TGFβ significantly reduces the expression of MUC5AC mRNA and its protein in NCI-H292 cells, a human mucoepidermoid carcinoma cell line. This reduced MUC5AC expression was restored by a TGFβ receptor inhibitor (SB431542), but not by the inhibition of NF-κB (BAY11-7082 or Triptolide) or PI3K (LY294002) activities. TGFβ-activated Smad3 dose-dependently bound to MUC5AC promoter. Notably, TGFβ-activated Smad3 recruited HDAC2 and facilitated nuclear translocation of HDAC2, thereby inducing the deacetylation of NF-κB at K310, which is essential for a reduction in NF-κB transcriptional activity. Both TGFβ-induced nuclear translocation of Smad3/HDAC2 and deacetylation of NF-κB at K310 were suppressed by a Smad3 inhibitor (SIS3). These results suggest that the TGFβ-activated Smad3/HDAC2 complex is an essential negative regulator for MUC5AC expression and an epigenetic regulator for NF-κB acetylation. Therefore, these results collectively suggest that modulation of the TGFβ1/Smad3/HDAC2/NF-κB pathway axis can be a promising way to improve lung function as a treatment strategy for asthma and COPD.
Ji-Eun Sung,Moon-Hwa Kwak,Ji-Eun Kim,Young-Ju Lee,Ro-Ui Kim,Eun-Ah Kim,Ga-Young Lee,Dong-Seob Kim,Dae-Youn Hwang 한국실험동물학회 2013 Laboratory Animal Research Vol.29 No.2
Atopic dermatitis (AD), which is known as the most common pruritic skin disease, is caused by epidermal barrier dysfunction, allergies, microwave radiation, histamine intolerance, and genetic defects. To investigate the therapeutic effects of fermented soycrud (FSC) on AD pathology, alteration of AD phenotypes induced by phthalic anhydride (PA) treatment was assessed by ear thickness analysis, measurement of immunerelated organ weights, ELISA, and histological and pathological analyses of ICR mice after FSC treatment for 2 weeks. Except for water content, the concentrations of most major components were lower in FSC compared to common tofu (CMT). Thymus and lymph node weights were significantly reduced in ICR mice treated with PA+CMT or PA+FSC, whereas spleen and body weights were maintained. Elevation of ear thickness induced by PA treatment was rapidly diminished in the CMT- and FSC-treated groups, although there was no significant difference between the two groups. Furthermore, significant reduction of epidermal thickness was detected in both the PA CMT- and PA+FSC-treated groups. However, IgE concentration and dermal thickness were reduced only by PA+FSC treatment, whereas PA+CMT treatment maintained levels comparable to PA+vehicle treatment. The number of infiltrated mast cells was higher in the PA+vehicle-treated group compared to the untreated control. Following CMT or FSC treatment, mast cell infiltration was slightly reduced, although the CMT-treated group showed greater cell numbers. These results indicate that FSC may significantly relieve the phenotypes of AD induced by PA treatment and should be considered as a potential candidate for AD therapy.
Hong, Gwan Ui,Cho, Jin Whan,Kim, Soo Youl,Shin, Joo Ho,Ro, Jai Youl Elsevier 2018 Toxicology and applied pharmacology Vol.358 No.-
<P><B>Abstract</B></P> <P>This study aimed to investigate the role of transglutaminase 2 (TG2) expressed in mast cells in substantia nigra (SN) in Parkinson's disease (PD) model or human PD patients. C57BL/6 mice received 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) by ip injection to induce PD. Bone marrow-derived mast cells (BMMCs) were adoptively transferred to TG2 knockout (KO or TG2<SUP>−/−</SUP>) mice by iv injection 1 day before MPTP injection or stimulated by 1 methyl-4-phenylpyridinium (MMP<SUP>+</SUP>). KO-MPTP mice showed reduced expression of tyrosine hydroxylase (TH) and dopamine (DA) transporter (DAT) and loss of TH<SUP>+</SUP> DA neurons, and expression of markers (c-kit, tryptase, FcεRI), mediators' release (histamine, leukotrienes, cytokines), and TG2 related to mast cells, and co-localization of DA neuronal cells and mast cells in SN tissues or release of mediators and TG2 activity in SN tissues and sera versus those in WT (wild type)-MPTP or BM + KO-MPTP mice. KO-MPTP mice reversed the alterations of behavior. KO-BMMCs-transferred KO-MPTP (BM + KO-MPTP) mice had restoration of all the responses versus the KO-MPTP mice. MPP<SUP>+</SUP>-stimulated BMMCs had increased mediators' release, which were inhibited by TG2 inhibitor (R2 peptide). All the mediators and TG2 activity were also increased in the sera of human PD patients. The data suggest that TG2 expressed in mast cells recruited into SN tissues might contribute to neuroinflammation, which is known as one of the important features in pathogenesis of PD, via up-regulating the release of various mediators.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Mast cells recruited into substantia nigra (SN) of PD, are activated by MPP<SUP>+</SUP>. </LI> <LI> The activated mast cells activate TG2 in the SN tissues of PD model in mice. </LI> <LI> Mast cells-activated TG2 releases mediators such as histamine, LTs and cytokines. </LI> <LI> Mediators may induce neuroinflammation caused DA neuron death in mouse and human PD. </LI> <LI> TG2 inhibitor may be developed as a therapeutic agent for human PD patients. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Lee, Su Ui,Lee, Seoghyun,Ro, Hyunju,Choi, Ji-Hee,Ryu, Hyung Won,Kim, Mun-Ock,Yuk, Heung Joo,Lee, Jinhyuk,Hong, Sung-Tae,Oh, Sei-Ryang Elsevier 2018 Phytomedicine Vol.40 No.-
<P>Conclusion: We propose that piscroside C is a promising therapeutic constituent of YPL-001 through its inhibition of PKC delta activity in the TNF-RSC/IKK/NF-kappa B/MUC5AC signaling cascade.</P>
Lee, Seoghyun,Ro, Hyunju,In, Hyun Ju,Choi, Ji-Hee,Kim, Mun-Ock,Lee, Jinhyuk,Hong, Sung-Tae,Lee, Su Ui Elsevier 2018 Cytokine Vol.108 No.-
<P><B>Abstract</B></P> <P>Fisetin (3,7,3′,4′-tetrahydroxyflavone), a natural flavonoid, is a therapeutic agent for respiratory inflammatory diseases such as chronic obstructive pulmonary disease (COPD). However, detailed molecular mechanisms regarding the target protein of fisetin remain unknown.</P> <P>Fisetin significantly reduces tumour necrosis factor alpha (TNF-α)-induced interleukin (IL)-8 levels by inhibiting both nuclear factor kappa B (NF-κB) transcriptional activity and the phosphorylation of its upstream effectors. We show that fisetin prevents interactions between protein kinase C (PKC)δ and TNF receptor-associated factor 2 (TRAF2), thereby inhibiting the inhibitor of kappa B kinase (IKK)/NF-κB downstream signalling cascade. Furthermore, we found that fisetin directly binds to PKCδ <I>in vitro</I>. Our findings provide evidence that fisetin inhibits the TNF-α-activated IKK/NF-κB cascade by targeting PKCδ, thereby mediating inflammatory diseases such as COPD.</P> <P>These data suggest that fisetin is a good therapeutic drug for the treatment of inflammatory lung diseases, such as COPD, by inhibiting the TNF-α/NF-κB signalling pathway.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Fisetin inhibits TNF-α-increased <I>IL-8</I> gene expression by blocking NF-κB activity. </LI> <LI> Fisetin inhibits TNF-α-mediated interactions between PKCδ and TRAF2. </LI> <LI> Fisetin inhibits PKCδ activity. </LI> </UL> </P>
B. subtilis MC 31를 이용한 청국장의 품질특성
맹소연(So-Yon Mann),김은아(Eun-Ah Kim),이가영(Ga-Young Lee),김로의(Ro-Ui Kim),황대연(Dae-Youn Hwang),손홍주(Hong-Joo Son),이병원(Byong-Won Lee),이충렬(Chung-Yeol Lee),김동섭(Dong-Seob Kim) 한국생명과학회 2013 생명과학회지 Vol.23 No.4
GABA 함량이 높은 청국장을 발효하는 Bacillus subtilis MC 31의 청국장 품질특성을 조사하였다. B. subtilis MC 31에 의해 발효된 청국장으로부터 24개의 아미노산이 검출되었고 그 중에서도 leucine이 가장 높은 함량을 나타냈다. B. subtilis MC31에 의해 발효가 된 청국장의 미생물의 생육 변화를 조사한 결과 총 균수가 정지기에 들어서면서 log 9.52±0.5~log 9.049±0.5 CFU/g까지 증가하였다. 청국장의 일반성분은 수분 61.7±0.01%, 회분 1.52±0.01%, 조단백 17.66±0.04%, 조지방 8.96±0.03%, 조섬유가 2.61%를 함유하였다. 암모니아태, 아미노태, 환원당은 모두 삶은 콩보다 청국장의 함량이 높게 조사되었지만 산도와 총 당은 청국장이 삶은 콩보다 함량이 낮았다. 청국장의 pH는 시간이 지날수록 알칼리화되고 점질물은 4.7±0.05%, protease activity는 0.519±7.36 g/l로 나타났으며 Fibrin plate와 Robbin실험을 통하여 fibrinolytic activity도 높음을 확인하였다. 이상의 결과로 미루어 보아 B. subtilis MC 31은 우수한 청국장 품질을 띄어 제조용 균주로 사용이 가능한 것으로 사료된다. Chungkookjang was fermented by B. subtilis MC31, a γ-amino butyric acid (GABA) producing microorganism. The characteristics of Chungkookjang were investigated while fermenting. Twenty four amino acids were detected in Chungkookjang, leucine was the highest of them all. Total cell populations of B. subtilis MC31 phase were between log 9.52±0.5 ~ log 9.049±0.5 CFU/g at stationary phase. Contents of moisture, crude ash, crude protein, crude lipid and crude fiber are 61.07±0.01%, 1.52±0.01%, 17.66±0.04%, 8.96±0.03% and 2.61%, respectively. Contents of ammonia type nitrogen, amino type nitrogen and reducing sugar were increased during fermentation at 40℃ for 72 hr, however those of titratable acidity and total sugar were decreased. pH was slowly alkalized during fermentation. Viscous substance and protease contents in Chungkookjang were 4.7±0.05% and 0.519±7.36 g/l, apiece. When the fibrin plate and Robbin method for fibrinolytic activity were applied, B. subtilis MC31 showed high activity. These results suggested that B. subtilis MC31 is suitable to be used as a starter to enhance the quality of Chungkookjang.