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Kim, Sun Ae,Park, Si Hong,Lee, Sang In,Ricke, Steven C. ACADEMIC PRESS LTD 2017 FOOD MICROBIOLOGY Vol.65 No.-
<P><B>Abstract</B></P> <P>A novel method was developed for the specific quantification of <I>S</I>. Typhimurium using a most-probable-number (MPN) combined with qPCR and a shortened incubation time (MPN-qPCR-SIT). For <I>S</I>. Typhimurium enumeration, dilutions of samples were transferred into three wells on a microtiter plate and the plate was incubated for 4 h. The <I>S</I>. Typhimurium presence in the wells was identified using a qPCR and populations were determined based on an MPN calculation. The R<SUP>2</SUP> between the MPN-qPCR-SIT and conventional MPN exhibited a high level of correlation (0.9335–0.9752), suggesting that the MPN-qPCR-SIT offers a reliable alternative method for <I>S</I>. Typhimurium quantification. Although plating and qPCR were limited in their ability to detect low levels of <I>S</I>. Typhimurium (e.g. 0.18 log MPN/ml), these levels could be successfully detected with the MPN-qPCR-SIT. Chicken breast samples inoculated with <I>S</I>. Typhimurium were incubated at 0, 4, and 24 h and incubated samples were subjected to microbiome analysis. Levels of <I>Salmonella</I> and Enterobacteriaceae increased significantly with incubation time. The obvious benefits of the MPN-qPCR-SIT are: 1) a further confirmation step is not required, 2) the detection limit is as low as conventional MPN, but 3) is more rapid, requiring approximately 7 h to simultaneously complete quantification.</P> <P><B>Highlights</B></P> <P> <UL> <LI> It is essential to develop more rapid and reliable quantification methods for <I>Salmonella.</I> </LI> <LI> We have developed a new quantification method for <I>S.</I> Typhimurium. </LI> <LI> The developed method demonstrated high accuracy and selectivity with a lower detection limit than the previous methods. </LI> <LI> This current method can be utilized to quantify <I>S.</I> Typhimurium by significantly reducing both time and labor. </LI> <LI> It may be particularly useful for the food industry and related applications where quantification is important. </LI> </UL> </P>
( Hye Lim Kwak ),( Sun Kyung Han ),( Sung Hoon Park ),( Si Hong Park ),( Jae Yong Shim ),( Mi Hwa Oh ),( Steven C Ricke ),( Hae Yeong Kim ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.9
Previous detection methods for Citrobacter are considered time consuming and laborious. In this study, we have developed a rapid and accurate detection method for Citrobacter species in pork products, using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). A total of 35 Citrobacter strains were isolated from 30 pork products and identified by both MALDI-TOF MS and 16S rRNA gene sequencing approaches. All isolates were identified to the species level by the MALDI-TOF MS, while 16S rRNA gene sequencing results could not discriminate them clearly. These results confirmed that MALDITOF MS is a more accurate and rapid detection method for the identification of Citrobacter species.