Microsatellite 를 이용한 돼지 Yorkshire 품종의 일당중체량 연관 DNA Marker 개발

송영민,김철욱,조광근,박외선,권은정,이정규,백동훈,최진성,노정만,홍연희 한국동물자원과학회 2000 한국축산학회지 Vol.42 No.4

This study was conducted to develop DNA markers related with average daily gain (ADG) using 27 pigs. Animals were divided into two groups: low ADG group had 11 pigs with 1.5 SD and high ADG group was consisted of 16 pigs with +1.5 SD. Initially, we choose and tested 78 microsatellites which could contain potential DNA markers for ADG. Part of the microsatellites were amplified by the polymerase chain reaction (PCR) and then electrophoresed on a agarose gel followed by the allelic, genotypic and statistical analysis. According to the alleic frequency analysis, 21 DNA polymorphisms were detected from 17 microsatellites and appeared to be associated with ADG (P$lt;0.05). From the genotypic frequency analysis, 14 DNA polymorphisms were found from II microsatellites and were associated with ADG. All pigs in the low ADG group had a 135:144 hetero type for the SWR308 marker, whereas all pigs in the high ADG group had a 144:144 homo type. This and possibly other selected markers could be useful for the selecting young pigs with an excellent growth potential.

Upregulation of CXCR4 is functionally crucial for maintenance of stemness in drug-resistant non-small cell lung cancer cells

Jung, M-J,Rho, J-K,Kim, Y-M,Jung, J E,Jin, Y B,Ko, Y-G,Lee, J-S,Lee, S-J,Lee, J C,Park, M-J Macmillan Publishers Limited 2013 Oncogene Vol.32 No.2

The hypothesis of cancer stem cells has been proposed to explain the therapeutic failure in a variety of cancers including lung cancers. Previously, we demonstrated acquisition of epithelial–mesenchymal transition, a feature highly reminiscent of cancer stem-like cells, in gefitinib-resistant A549 cells (A549/GR). Here, we show that A549/GR cells contain a high proportion of CXCR4+ cells that are responsible for having high potential of self-renewal activity in vitro and tumorigenicity in vivo. A549/GR cells exhibited strong sphere-forming activity and high CXCR4 expression and SDF-1α secretion compared with parent cells. Pharmacological inhibition (AMD3100) and/or siRNA transfection targeting CXCR4 significantly suppressed sphere-forming activity in A549 and A549/GR cells, and in various non-small cell lung cancer (NSCLC) cell lines. A549/GR cells showed enhanced Akt, mTOR and STAT3 (Y705) phosphorylation. Pharmacological inhibition of phosphatidyl inositol 3-kinase or transfection with wild-type PTEN suppressed phosphorylation of Akt, mTOR and STAT3 (Y705), sphere formation, and CXCR4 expression in A549/GR cells, whereas mutant PTEN enhanced these events. Inhibition of STAT3 by WP1066 or siSTAT3 significantly suppressed the sphere formation, but not CXCR4 expression, indicating that STAT3 is a downstream effector of CXCR4-mediated signaling. FACS-sorted CXCR4+ A549/GR cells formed many large spheres, had self-renewal capacity, demonstrated radiation resistance in vitro and exhibited stronger tumorigenic potential in vivo than CXCR4− cells. Lentiviral-transduction of CXCR4 enhanced sphere formation and tumorigenicity in H460 and A549 cells, whereas introduction of siCXCR4 suppressed these activities in A549/GR cells. Our data indicate that CXCR4+ NSCLC cells are strong candidates for tumorigenic stem-like cancer cells that maintain stemness through a CXCR4-medated STAT3 pathway and provide a potential therapeutic target for eliminating these malignant cells in NSCLC.

BELLE TOF 검출기의 특성 연구

남지우,김동욱,배미영,박강순,김정현,노상률,최영일,김희종,장동욱,최소명,권영준,Hikita, S.,Yu, C.,Yoshimura, Y.,Kichimi, H. 성균관대학교 기초과학연구소 1998 論文集 Vol.49 No.-

We investigated the properties of BELLE Time Of Flight(TOF) counter using the data taken in Jan 98 beam test at Pi2 beam line at KEK 12 GeV proton synchrotron. Charge distributions from readout PMT were analysed in comparison with dE/dx distributions predicted by GEANT full simulation. Experimental setup of the beam test is described, then procedures of data analysis, Monte Carlo simulation and analysis results are also described.

葉煙草에 關한 微量要素 效果 試驗(第一報)

盧載榮,裵吉寬 충북대학교 엽연초연구소 1971 煙草硏究 Vol.1 No.-

1.This experiment has been carried out in order to study the effects of minor-elements fertilizer on the tobacco plants. 2.The soil used in this experiment was in such a way that the lacks of Boron has been frequently apeared for some vegetables, especially for Radish or Chines cabbages, but not for tabacco plants. 3.As the result of this experiment, it was noticed that the Boron fertilizer promoted significantly both the yield of tobacco leaves and the quality of them (used 2kg per 10a) 4.The further studies on the application of Boron fetilizer-Soil tests in tobacco producing districts and analyzing fresh leaves of tobacco in order to inspect the quantity of available Borex will have to be contained and completed

항공기 재료의 표면처리 현황 및 특성분석

권식철,송요승,변응선,한주철,이구현,이상로,윤우생,노병호 국립경상대학교 공과대학 부설 첨단소재연구소 1995 尖端素材 Vol.5 No.-

돼지고기를 단백질 급원으로 한 식이가 쥐의 체내 카드뮴 대사에 미치는 영향에 대한 연구

정용기,노정해,이남형,한찬규 한국축산학회 1997 한국축산학회지 Vol.39 No.5

This study was performed to investigate the effect of feeding pork in the place of casein on cadmium toxicity in rats. Thirty six male rats of Sprague-Dawley weighing 225.2?9.3g were randomly blocked into six groups according to body weights. Three levels of cadmium in drinking water(0, 25 and 50 ppm) had been fed for 5 weeks. Pork replaced dietary casein as a protein source. Weight gains in either cadmium-added or pork-fed group tended to be lower than those of the control group(Cd 0 ppm and casein). The cadmium addition and pork feedings affect significantly feed efficiency according to factor analysis results. Hematocrit was decreased with cadmium intake(p$lt;0.05). Cadmium accumulation in liver showed a tendency to diminish in the pork-fed group among the cadmium-exposed groups. Hepatic enzyme activity of glutatione peroxidase and superoxide dismutase showed no significant differences among the treatments. Urinary and fecal cadmium excretions increased with pork feeding. The results indicate that pork feeding as protein source in diet may alleviate the cadmium toxicity in rats.

흡입 분만 후 태아 뇌출혈 1예

박찬용,박창서,박인서,송경록,김영석,노은배 한국의학사 1987 診斷과治療 Vol.7 No.7

산란계의 연령과 면역주기에 따른 난황 중의 Streptococcus mutans 특이 항체 함량

성기승,김영붕,노정해,이남형,한찬규,손동화 한국축산학회 1999 한국축산학회지 Vol.41 No.5

Streptococcus mutans-specific IgY content change, laying rate, egg weight and weight change were measured for 17-week and 30-week old hens. Vaccinations with Streptococcus mutans were made two times(eight week interval), three times(four week interval) and five times(two week interval), respectively. It was observed that the laying rate of vaccinated hens was likely lower than that of non-vaccinated group. No effect on body weight by vaccination was found out. Egg weight did not show a certain tendency by vaccination. Anti-S. mutans IgY started to be detected two weeks after the lst vaccination for 30-week old hens. It was not detected for non-vaccinated group. The antibody activity was consistently detected after 8 weeks from the last vaccination. The measurement of total IgY and S. mutans-specific IgY in the egges from vaccinated hens revealed that IgY tended to increase with the number of vaccination. S. mutans-specific IgY content of five-time vaccinated l7-week hens was much higher than that of 30-week old hens. To obtain steady amount of specific IgY, multiple vaccination with two week interval was recommended.

지방산 결합단백질에 관한 연구 동향

권영아,노숙령,김혜경,함영태 中央大學校 食糧資源硏究所 1996 食糧資源硏究所 論文集 Vol.8 No.1

ABSTRACT Long chain free fatty acid (FFA) are the main energy source for many mammalian cell, and FFA metabolism is dependent upon the flux of FFA to sites of intra-cellular processing. Fatty Acid Binding Proteins (FABPs) are a family of abundant cytosolic proteins of low M.W.14-15 kDa. Although their precise physilogical role remains hypothetical, it has been proposed as a FFA transfer protein and as a binding protein responsible for controlling intracellular FFA concentration. Furthermore, significant correlations have been observed between the tissue content of FABP and the rate of FFA uptake or utilization in several different manipulations such as diet and hypolipidemic drugs. However, to date there has been no report which examines the role of FABP in physiological manipulations such as fatty liver, diabetes, obesity, hypercholesterolemia. Since lipid metabolism is abnormal and the liver is greatly affected in these disease, it is possible that FABP may be abnormal in these livers. Therefore, the realtionships of liver FABP and the disease with lipid abnormalities, especially fatty liver induced by alcohol and high cholesterol diet are examined in our lab. Where FABP is believed to be involved in FFA binding and transfer, cellular FABP amount as well as its binding activity are affected. If we further find the way to increase the expression of FABP in liver, it could be one means of settle for these kinds of diseases from lipid abnormality.

방사성동위원소심혈관조영술상 관찰된 주폐동맥유와 동맥관개존증이 병발된 1예

조보연,이명철,고창순,한만청,손인,윤용수,홍창의,노준량,연경모 대한핵의학회 1981 핵의학 분자영상 Vol.15 No.2

A Case of main pulmonary artery aneurysm in a 9-year-old boy with patent ductus arteriosus is presented. In this case presented with a huge mass density on the chest X-ray, radionuclide cardiac angiography showed a vascular lesion, which was confirmed as an aneurysm of the main pulmonary artery at roentgenologic angiogram. The aneurysm appeared following an episode of bacterial endocarditis and pulmonary hypertension. A successfu1 aneurysmectomy with multiple ligation of ductus arteriosus was performed.